Regulation of actin filament dynamics. Medical search. Frequent questions

Chapter 1 provides an overview of these pathways including the key steps of DSB repair, the regulation of actin nucleation, and the proteins involved in chromatin mobility. (columbia.edu)
Using Xenopus laevis cell-free extracts and mammalian cells, we show that actin nucleation machinery (WASP, Arp2/3, and actin) is recruited to damaged chromatin undergoing HDR. (columbia.edu)
Actin forms the dendritic nucleation network and undergoes rapid polymerization-depolymerization cycles in lamellipodia. (silverchair.com)
Actin nucleation triggers the formation of new actin filaments and has the power to shape cells but requires tight control in order to bring about proper morphologies. (healthanddietblog.info)
The rate-limiting step in actin polymerization is "nucleation"-a process during which the first actin monomers are assembled with the help of actin nucleators. (healthanddietblog.info)
In vitro reconstituted systems will be used to control the actin nucleation patterns, the closed volume of the reaction chamber and the physical interaction of the networks. (hipi-lab-saint-louis.fr)
Second, we will use our unique capacity to perform dynamic micropatterning, to add or remove actin nucleation sites in real time, in order to investigate the ability of dynamic networks to adapt to changes and the role of coupled network dynamics in this emergent property. (hipi-lab-saint-louis.fr)
The ability of VASP to contribute to actin filament nucleation and to regulate actin filament architecture highlights the central role of VASP in actin-based motility. (silverchair.com)

Recent studies have proposed roles for cytoskeletal proteins in genome organization and chromosomal dynamics. (columbia.edu)
To elucidate the mechanism of actin disassembly, we characterized molecular kinetics of the major filament end-binding proteins Arp2/3 complex and capping protein (CP) using single-molecule speckle microscopy. (silverchair.com)
Memory and learning involve activity-driven expression of proteins and cytoskeletal reorganisation at new synapses, often requiring post-transcriptional regulation a long distance from corresponding nuclei. (ucl.ac.uk)
Actb , Acta1 ) and actin-binding proteins (e.g. (jneurosci.org)
Elucidate the structure, dynamics and functions of intrinsically disordered proteins and protein regions (IDPs/IDRs) and their biological regulation by Post-translational modifications. (upstate.edu)
Proteins of the Sla2p/Hip1R family bind to actin and clathrin at endocytic sites in yeast and mammals. (rupress.org)
These vesicles contain the mannose 6-phosphate receptor involved in targeting proteins to the lysosome, and the actin nucleating Arp2/3 complex. (rupress.org)
GI:203255) because CaM kinases (CaMKs) downstream of CaM were observed to be involved in dendritogenesis [4 5 Prime effector machinery that may power early neuromorphogenesis would be proteins with the ability to trigger the formation of new actin filaments in a spatially and locally well-controlled manner. (healthanddietblog.info)
Among the nineteen identified proteins, seven were molecular chaperones, five were actin PKCε Modulator Gene ID filaments or related proteins, and two had been involved in αvβ3 Antagonist MedChemExpress energy production (Table 1). (betadesks.com)
We surmise that these proteins collectively are involved in (1) protein modifications and membrane dynamics required to prepare the plasma membrane for cell-cell interactions (i.e., the molecular chaperones) and (two) regulation of membrane trafficking and phagocytosis by actin filaments. (betadesks.com)
As both actin and GTPase have been very biotinylated (see the "Relative ratio (folds) of biotinylated vs total proteins" column in Table 1. (betadesks.com)
The contributors discuss the 3D organization of chromatin, the various nuclear bodies and compartments that have been identified, and the roles of RNA and actin in shaping nuclear organization, as well as how these structures interact with each other and with peripheral features (e.g., the nuclear pore complex and inner nuclear membrane proteins) to carry out the work of the nucleus. (cshlpress.com)
Bin/Amphiphysin/Rvs (BAR) proteins are peripheralmembrane proteins (PMP) and play an important role in sculpturingmembranes and in the regulation of actin dynamics. (kth.se)
Thisimplies that BIN1 rather bundles actin than decorates single filaments.Third, we explored a strategy to purify an aggregation prone BAR protein.Aggregation is a property common in Peripheral Membrane Proteins. (kth.se)
Cell tension is generated along the actin filaments by the movement of myosin II motor proteins along the filaments (see contractile bundles). (mechanobio.info)
and proteins that interact with members of the Arp2/3 complex and hence the actin cytoskeleton (Table 1 ). (biomedcentral.com)
It comprises three major filament systems-actin, microtubules, and intermediate filaments-along with a host of adaptors, regulators, molecular motors, and additional structural proteins. (cshlpress.com)
We investigate the role of proteins associated to cell adhesions and actin cables as well as the effect of actin cable's size, number and spatial organization in cells. (cerclefser.org)

The cytoskeleton of a cell is made up of microtubules, actin filaments, and intermediate filaments. (renest.best)
Learn how microtubules, actin filaments, and intermediate filaments organize the cell. (renest.best)
The cytoskeleton is made up of 3 protein filaments: actin filaments (also known as microfilaments), intermediate filaments and microtubules. (renest.best)
Recent studies suggest that intermediate filaments undergo reorganization during migration, which coordinates focal adhesion dynamics, cell contraction, and nucleus rigidity. (renest.best)
In particular, vimentin intermediate filaments undergo phosphorylation and reorientation in smooth muscle cells, which may regulate cell contraction and focal adhesion assembly/disassembly. (renest.best)
Intermediate filaments (IFs) are components of the cytoskeleton involved in most cellular functions including cell migration. (biongenex.com)
Introduction The cytoskeleton composed of actin microfilaments microtubules and intermediate filaments (IFs) is a fundamental element of eukaryotic cells. (biongenex.com)

They play a crucial role in the regulation of cytoskeleton dynamics by controlling actin filaments and microtubule turnover, especially through the phosphorylation of cofilin, an actin depolymerising factor. (bvsalud.org)
These tools allow us to analyze and quantify actin and microtubule networks in cells of controled and regular shapes. (hipi-lab-saint-louis.fr)
2008 APC contributes to cell migration through the regulation of the actin and microtubule cytoskeletons (Etienne-Manneville 2009 Although two APC isoforms exist (APC and APC2) only APC is expressed in astrocytes (Cahoy et al. (biongenex.com)

CP dissociates slower from actin stress fibers than from the lamellipodial actin network, suggesting that CP dissociation correlates with actin filament dynamics. (silverchair.com)
Cells exert traction forces on the ECM and generate tension at focal adhesions through actin stress fibers , which are higher-order structures in the cytoplasm that consist of parallel contractile bundles of actin and myosin filaments. (mechanobio.info)
Actin networks can self-organize into two main types of structures in cells: bundles (or fibers) made of aligned long filaments and meshworks made of branched and intermingled short filaments. (cerclefser.org)

Our data demonstrate that neurotrophin binding to p75 NTR is necessary and sufficient to regulate filopodial dynamics. (jneurosci.org)
These observations suggest that neurotrophins regulate filopodial dynamics by depressing the activation of RhoA that occurs through p75 NTR signaling. (jneurosci.org)
The signaling pathways activated by neurotrophins can regulate cytoskeletal dynamics, which in turn determine growth cone behaviors (Gallo and Letourneau, 1998 , 2000 ). (jneurosci.org)
The Rho family of GTPases regulate the actin cytoskeleton. (jneurosci.org)
Apart from their roles in signal transduction and protein biosynthesis, Rho family GTPases have also been shown to regulate the actin cytoskeleton and cell adhesion via particular targets in mammalian cells [31]. (betadesks.com)
Guidance cues activate signaling transduction pathways that, in turn, regulate the dynamics and organization of the neuronal cytoskeleton. (biologists.com)
Our aim is to reconstitute actin networks in vitro and investigate : 1- how the spatial distribution of actin nucleators directs the growth and assembly of filaments to form defined network geometries in 2D and 3D, 2- how the contraction and disassembly processes regulate networks turn-over and permanent renewal. (cerclefser.org)

Our in vitro assays show that the R21H mutation causes a twofold decrease in tropomyosin's affinity for F-actin and affects leiomodin's function. (nih.gov)
In this part, in vitro experiments will be complemented by the analysis of actin network remodeling in living cells. (hipi-lab-saint-louis.fr)
Feng S, Chen N, Huang S. Mechanism of CAP1-mediated apical actin filaments in vitro in the brain that determines when each component of u and v reflects the average length of the BiComp-DTA against that of Filtered-OFDM (F-OFDM). (absolute-forwarding.ru)

In addition to regulating cytoskeletal dynamics in the cytoplasm, actin modulates nuclear gene expression. (jneurosci.org)
In addition to regulation of cytoplasmic cytoskeletal dynamics, a little appreciated property of actin signaling is modulation of gene expression so far only reported for non-neuronal cells. (jneurosci.org)
Actin forms microfilaments in eukaryotic cells that are the major component of the cytoskeletal system. (pte.hu)
Actin Cytoskeletal Dynamics in the leukocyte inflammatory phenotype. (upstate.edu)
Hence, correct cytoskeletal regulation is crucial for the control of cell behaviour, such as cell division and migration. (iciam2023.org)

Neuronal motility relies on actin treadmilling. (jneurosci.org)
We present a hitherto unappreciated cross talk of actin signaling with gene expression governing neuronal motility. (jneurosci.org)
We show that neuronal motility evoked by these actin mutants requires SRF activity. (jneurosci.org)
Moreover, R62D NLS decreases neuronal motility similar to the cytoplasmic R62D actin mutant although R62D NLS has no access to cytoplasmic actin dynamics. (jneurosci.org)
Thus, herein we provide first evidence that neuronal motility not only depends on cytoplasmic actin dynamics but also on the availability of actin to modulate nuclear functions such as gene transcription. (jneurosci.org)
Employing these actin mutants, we uncovered a function for actin signaling in growth-cone motility modulated by ephrin-A guidance cues. (jneurosci.org)
Reorganization of the actin cytoskeleton plays crucial roles in many cellular functions, including morphology, motility, and adhesion. (biomedcentral.com)
The Listeria monocytogenes ActA protein mediates actin-based motility by recruiting and stimulating the Arp2/3 complex. (silverchair.com)
however, this region is dispensable for actin-based motility in cells. (silverchair.com)

Formation of axonal F-actin bundles was independent of myosin II, but partially required RhoA-kinase activity. (biologists.com)

The Arp2/3 complex generates propulsive forces by nucleating a highly branched network of actin filaments. (columbia.edu)
In this chapter, I also investigate how Arp2/3-driven DSB movements specifically promote the dynamics of HDR breaks, while Arp2/3 activity does not influence NHEJ breaks. (columbia.edu)
The well-established actin filament-promoting components i.e. the Arp2/3 Formins and complex are controlled by Rho-type GTPases [6-9]. (healthanddietblog.info)
Furthermore, purified VASP enhances the actin-nucleating activity of wild-type ActA and the Arp2/3 complex while also reducing the frequency of actin branch formation. (silverchair.com)

The dynamic actin cytoskeleton spatially and temporally regulates protrusion, adhesions, contraction, and retraction from the cell front to the rear. (renest.best)
We hope to unravel the mechanism allowing the global coherence of a dynamic actin cytoskeleton. (hipi-lab-saint-louis.fr)

Filopodia are finger-like projections supported by a cytoskeleton of bundled actin filaments ( Gallo and Letourneau, 2000 ). (jneurosci.org)
G15S actin enhanced neurite outgrowth and filopodia number. (jneurosci.org)
Furthermore, semaphorin 3A promotes the formation of intra-axonal F-actin bundles in concert with the loss of F-actin in growth cone lamellipodia and filopodia. (biologists.com)

Genotoxic agents trigger actin polymerization in the nucleus. (columbia.edu)
Chapter 1 provides context for the rest of the thesis in which I explore the contribution of nuclear actin polymerization to DSB repair. (columbia.edu)
Here we analyzed neuronal actin signaling by a novel approach using actin mutant isoforms known to favor or preclude actin polymerization. (jneurosci.org)
Author Summary The organization and the formation of new actin filaments by polymerization of actin monomers has the power to shape cells. (healthanddietblog.info)
CryoEM reveals that themuscular BIN1 isoform does not bind to single actin filaments, althoughBIN1 can be co-sedimented with actin after polymerization of actin. (kth.se)
In all systems, these GTPases are best known for their effects on the polymerization and distribution of actin in the cell cortex [ 1 ]. (biomedcentral.com)
Conversely, RhoA-kinase activity was required to shut down F-actin polymerization underlying protrusive activity. (biologists.com)
Intracellular L. monocytogenes replicate in the cytoplasm of host cells and induce the polymerization of host actin filaments at the bacterial surface. (silverchair.com)
Polarized polymerization of actin propels the bacteria through the cytoplasm and into pseudopod-like structures that are engulfed by neighboring cells. (silverchair.com)

Notably, a nucleus-resident actin (R62D NLS ) also regulates SRF's transcriptional activity. (jneurosci.org)

Conversely, actin signaling regulates neuronal SRF-mediated gene expression. (jneurosci.org)
By unveiling the means of Cobl regulation and the mechanisms by which Ca2+/CaM signals directly converge on a cellular effector promoting actin filament formation our work furthermore sheds light on how local Ca2+ signals steer and power branch initiation during early arborization of nerve cells-a key process in neuronal network formation. (healthanddietblog.info)
Here we SNX-2112 analyse signaling cascades and mechanisms regulating the actin nucleator Cobl which is crucial for the formation of dendritic arbors of nerve cells-a key process in neuronal network formation. (healthanddietblog.info)

We use a wide range of biophysical, biochemical, cell biological, and genetic approaches to uncover the general principles underlying regulation of the actin cytoskeleton, and to reveal how defects in actin dynamics affect the physiological functions of cells. (helsinki.fi)
Here, we provide genetic and biochemical evidence that vasodilator-stimulated phosphoprotein (VASP) recruitment by ActA can bypass defects in actin monomer-binding. (silverchair.com)

Apr 8, 2017 · c-Abl tyrosine kinase plays a critical role in regulating actin dynamics and migration of airway smooth muscle cells and nonmuscle cells. (renest.best)

Recently, we have shown that TWD1is involved in the regulation of actin filament organization and dynamics. (unifr.ch)
The organization and dynamics of actin filament must be precisely controlled during these processes, and consequently defects in regulation of the actin cytoskeleton lead to various diseases including cancer progression, as well as neurological and immunological disorders. (helsinki.fi)
Introduction Metazoan life critically relies on the formation organization and dynamics of actin filaments which are for example crucial for shaping and movement of membranes and entire cells. (healthanddietblog.info)

Unconventional myosin-Va is a motor protein in charge of the intracellular transport of vesicles, organelles and protein complexes along the actin filaments. (wikipedia.org)
The interactions of bipolar myosin II filaments with actin arrays are a predominate means of generating forces in numerous physiological processes including muscle contraction and cell migration. (aps.org)
transitions between these regimes occur by changes to duty ratio and myosin filament size. (aps.org)
The molecular motor myosin can induce constriction of the cell by moving actin filaments against each other. (iciam2023.org)
Capturing and quantifying these interactions between myosin and actin in living cells is an ongoing challenge. (iciam2023.org)
Collectively, these observations suggest that guidance cues cause axon retraction through the coordinated activation of myosin II and the formation of intra-axonal F-actin bundles for myosin-II-based force generation. (biologists.com)
Myosin II interacts with F-actin to generate contractile forces that result in axon retraction. (biologists.com)
how can myosin II drive axon retraction if the major source of the required substratum for force generation, growth cone F-actin, has been depleted? (biologists.com)

We used Förster resonance energy transfer to study effects of the tropomyosin mutations on the structure and kinetics of the cardiac troponin core domain associated with the Ca 2+ -dependent regulation of cardiac thin filaments. (nih.gov)
We found that the K15N mutation desensitizes thin filaments to Ca 2+ and slows the kinetics of structural changes in troponin induced by Ca 2+ dissociation from troponin, while the R21H mutation has almost no effect on these parameters. (nih.gov)

Six-domain gelsolin regulates actin structural dynamics through its abilities to sever, cap and uncap F-actin. (a-star.edu.sg)

As a result, most of LIMK2's physiological and pathological roles have been assigned to its regulation of actin dynamics via cofilin. (bvsalud.org)
Cofilin stimulates actin depolymerization and severance of actin filaments at the protruding ends, thereby promoting their rapid turnover and determining the direction of cell movement [ 4 - 5 ]. (oncotarget.com)

This is particularly true for the actin cytoskeleton, a major component of the internal architecture of eukaryotic cells. (hipi-lab-saint-louis.fr)

By regulating filamentous actin, Cdc42 and Rac exert a profound effect on cell shape, polarity, migration, cell:cell and cell:matrix adhesion, protein traffic, and cytokinesis. (biomedcentral.com)

The regulation of microfilaments and microtubules has received a great deal of attention (Bugyi and Carlier 2010 Etienne-Manneville 2010 Lee and Dominguez 2010 In contrast the regulatory mechanisms of cytoplasmic IF rearrangements are still poorly characterized. (biongenex.com)

In diverse species, actin assembly facilitates clathrin-coated vesicle (CCV) formation during endocytosis. (rupress.org)

Pan-cancer analysis and scRNA-seq data analysis were employed to forecast the association between Actin filament-associated protein 1 like 1 (AFAP1L1) and the development of tumors and endothelial cells. (biomedcentral.com)
In summary we i) show a direct regulation of the actin nucleator Cobl by Ca2+/CaM ii) demonstrate that the molecular mechanisms we discovered are crucial for shaping nerve cells SNX-2112 and iii) underscore how local Ca2+ signals steer and power branch initiation during early arborization of neurons. (healthanddietblog.info)
Dynamic networks of protein filaments give shape to cells and power cell movement. (renest.best)
Finally, we found that transcription factors Jun , Fos , Atf3 , and Egr1 were upregulated in protective cells, especially Egr1 was predicted to be involved in the regulation of genes related to antioxidant stress and angiogenesis, suggesting a role in promoting differentiation into this cell phenotype. (hindawi.com)
it is initiated by membrane protrusion, which is mediated by the dynamics of actin filaments [ 1 - 2 ] that assemble as a network at the leading edge of migrating cells [ 3 ]. (oncotarget.com)
Cells migrate effectively if actin turnover is included with a viscoelastic description for the cortex. (iciam2023.org)
To that end we use microfabrication tools to impose spatial boundary conditions to individual cells or purified cytoskeleton filaments. (hipi-lab-saint-louis.fr)
Actin filaments and microtubules form such complex intricate networks in cells that it is difficult to identify the principles of their self-organization. (hipi-lab-saint-louis.fr)
In living cells, actin networks constantly assemble and disassemble filaments while maintaining an apparent stable structure, suggesting a perfect balance between the two processes. (hipi-lab-saint-louis.fr)
They confer upon actin networks a high degree of plasticity allowing them to adapt in response to external changes and enable cells to adjust to their environments. (hipi-lab-saint-louis.fr)
In cells, F-actin assumes specific types of organization depending on its functions. (biologists.com)
Indeed, cells have a skeleton made of filaments. (cerclefser.org)

Mechanistic studies revealed that Ca2+/CaM modulates Cobl's actin binding properties and furthermore promotes Cobl's previously identified interactions with the membrane-shaping F-BAR protein syndapin I which accumulated with Cobl at nascent dendritic protrusion sites. (healthanddietblog.info)
We find that Ca2+/CaM modulates Cobl's actin-binding properties and promotes its interactions with syndapin I which then serves as a membrane anchor for Cobl. (healthanddietblog.info)
BG modulates the dynamics and spatial distribution of the locations where exogenous input is then deactivated and the training and inference on GPUs for both models. (absolute-forwarding.ru)

Tropomyosin, together with the troponin complex, regulates muscle contraction and, along with tropomodulin and leiomodin, controls the uniform thin-filament lengths crucial for normal sarcomere structure and function. (nih.gov)
The findings of our study demonstrate a direct regulation of an actin nucleator by Ca2+/CaM and reveal that the Ca2+/CaM-controlled molecular mechanisms we discovered are crucial for Cobl's cellular functions. (healthanddietblog.info)
These results point to APC Rilmenidine as a crucial regulator of IF organization and confirm its fundamental role in the coordinated regulation of cytoskeletons. (biongenex.com)

MY5A perform an important role in the regulation of axonal vesicle transport on the neurofilaments. (wikipedia.org)
The establishment of neural circuits depends on the ability of axonal growth cones to sense their surrounding environment en route to their target. (biologists.com)

Cdc42 and Rac - ancient, highly conserved, small GTPases - mediate extracellular signals, triggering changes in transcription and in the actin cytoskeleton. (biomedcentral.com)

To further analyze neurotrophin regulation of growth cone behaviors, we examined the role of p75 NTR in regulating filopodial dynamics in peripheral and CNS neurons. (jneurosci.org)
Overexpressing these mutant actins in mouse hippocampal neurons not only modulated growth-cone function but also neurite elongation, which was ambiguous by traditional pharmacological interference. (jneurosci.org)
Growth-cone collapse induced by ephrin-As, a family of repulsive axon guidance molecules, is impaired upon R62D expression, resulting in perseverance of ring-shaped F-actin filaments. (jneurosci.org)
F-actin is concentrated at the growth cone and sparse in the axon ( Letourneau, 1983 ). (biologists.com)
Importantly, repellent guidance cues cause growth cone collapse and depolymerize growth cone F-actin while promoting axon retraction ( Gallo and Letourneau, 2004 ). (biologists.com)

Expression of the K15N mutant in cardiomyocytes decreases leiomodin's thin-filament pointed-end assembly but does not affect tropomodulin's assembly at the pointed end. (nih.gov)
Finally, we will assess the role of y-actin in costamere assembly and mechanical function by characterizing new lines of mice where it is specifically ablated in striated muscle. (grantome.com)
Toward this end, we used a novel approach using mutant actins either favoring (G15S) or inhibiting (R62D) F-actin assembly. (jneurosci.org)
The actin mutants G15S and S14C favor F-actin assembly, yet differ with regard to their ABP interaction. (jneurosci.org)
We hypothesized that Hip1R might also coordinate actin assembly with clathrin budding at the trans-Golgi network (TGN). (rupress.org)
The spatial organization of these filaments and their dynamics of assembly direct cell shape and cell motion. (cerclefser.org)
Similar to the formation of actin filaments from the self-assembly of actin monomers, tubulin forms asymmetric dimers that can self-assemble into microtubules. (cerclefser.org)

Silencing of Hip1R expression by RNAi resulted in disruption of Golgi organization and accumulation of F-actin structures associated with CCVs on the TGN. (rupress.org)
Insights into DNA replication timing and RNA processing dynamics based on new technologies aimed at examining chromatin and other intranuclear structures at high resolution are also included. (cshlpress.com)
To understand how this gelsolin fragment is activated for F-actin severing by lowering pH, we solved its NMR structures at both pH 7.3 and 5 in the absence of Ca2+ and measured the pKa values of acidic amino acid residues and histidine residues. (a-star.edu.sg)

Actin nucleators that respond to Ca2+/CaM signals are not known directly. (healthanddietblog.info)

Moreover, emerging studies have unveiled a few tumor suppressors and oncogenes as LIMK2's direct substrates, which in turn have uncovered novel molecular mechanisms by which it plays pleiotropic roles in human physiology and pathologies independent of actin dynamics. (bvsalud.org)
The present study identified three actin protein spots, with inferred molecular weights ranging from 44 to 47 kDa and pIs from five.two to six.0 (Table 1). (betadesks.com)

The cytoskeleton is the intracellular filament system that controls the morphology of a cell, allows it to move, and provides trafficking routes for intracellular transport. (cshlpress.com)

These studies establish roles for Hip1R and actin in CCV budding from the TGN for lysosome biogenesis. (rupress.org)

Cobl-induced dendritic branch initiation was preceded by Ca2+ SNX-2112 signals and coincided with local CaM and F-actin accumulations. (healthanddietblog.info)
We observed local accumulation of CaM Cobl actin and syndapin I-a membrane-shaping protein-at dendritic branch initiation sites. (healthanddietblog.info)

SRF controls gene transcription of various actin isoforms (e.g. (jneurosci.org)

Moreover these actin mutants affect neurite elongation, an actin function which by pharmacological actin interference was ambiguous. (jneurosci.org)

Interestingly, inhibiting specifically DGK changed vacuolar dynamics and the morphology of pollen tubes, whereas inhibiting specifically PLD disrupted the actin cytoskeleton. (frontiersin.org)
Actin filament remodeling at cell surfaces is fundamental to regulating membrane elasticity and cell morphology [29,30]. (betadesks.com)

Regulation of organ size is essential in animal development, and Hippo (Hpo) signaling is a major conserved mechanism for controlling organ growth. (sdbonline.org)
This study provides insights into the mechanism of Tao-1 regulation and a potential growth control function for SCHIP1 in mammals. (sdbonline.org)
The principal mechanism of angiogenesis inhibition entails the regulation of tip cell behavior through the YAP-DLL4-NOTCH axis. (biomedcentral.com)
mechanism that requires a combination of all three of its actin monomer-binding WH2 domains. (healthanddietblog.info)

In this proposal, we will test the novel hypotheses that increased y-actin may directly alter the activity of other muscle cell constituents involved in signaling, gene expression, or contractility. (grantome.com)

This function appears to be evolutionary conserved since TWD1 expression in budding yeast alters actin polarization and cell polarity and provides NPA sensitivity. (unifr.ch)

Phalloidin, a cyclic peptide fromAmanita phalloides can tightly bind to actin filaments and stabilizes their structure (Faulstich et al. (pte.hu)

TWD1 serves as a chaperone during ER to PM secretion of auxin-transporting ABCBs: in analogy to regulation of Cystic Fibrosis Conductance Regulator (CFTR/ABCC7) by human FKBP38, ABCBs are degraded in the absence of TWD1/FKBP42. (unifr.ch)
We will characterize new transgenic mouse lines to determine which dystrophy phenotypes may be caused by increased myoplasmic y-actin concentration in the absence of sarcolemmal damage. (grantome.com)
The fragment comprising the first domain and six residues from the linker region into the second domain has been shown to be similar to the full-length protein in F-actin severing activity in the absence of Ca2+ at pH 5. (a-star.edu.sg)

Yet, the mechanisms underlying the regulation of filopodial dynamics are not well understood. (jneurosci.org)
We suggest that the K15N mutation causes DCM by altering Ca 2+ -dependent thin-filament regulation and that one of the possible HCM-causing mechanisms by the R21H mutation is through alteration of leiomodin's function. (nih.gov)
Despite their fundamental importance in the regulation of cell physiology, the basic mechanisms that control the coordinated dynamics of co-existing actin networks are poorly understood. (hipi-lab-saint-louis.fr)

Actin is an asymmetric protein that can self-assemble to form polarized actin filaments. (cerclefser.org)

This retraction requires actin dynamics and is likely to be caused by the retrograde flow of actin filaments emerging from motile cell edges (Bershadsky et al. (biongenex.com)
A requirement for actin filaments (F-actin) in mediating axon retraction has long been established ( Solomon and Magendantz, 1981 ). (biologists.com)

We are affiliated to Cell and Tis-sue Dynamics and Biomolecular structure and function Research Programs. (helsinki.fi)
He is affiliated to Cell and Tissue Dynamics and Structural and Quantitative Biology Research Programs. (helsinki.fi)
While an increase in the y-actin monomer pool likely stabilizes costameric filaments by mass action, excess myoplasmic y-actin may have adverse consequences for muscle cell function. (grantome.com)
In recent years, owing to the swift advancement of high-throughput sequencing technology, pan-cancer analysis and single-cell sequencing analysis have emerged as pivotal methodologies and focal areas within the domain of omics research, which is of great significance for us to find potential targets related to the regulation of pathological neovascularization. (biomedcentral.com)
Basically, it's like a network of tiny filaments that reach all over the cell. (renest.best)
Multifunctional chaperones: cell-cell recognition and regulation of membrane dynamics. (betadesks.com)
Is the F-actin-binding protein drebrin important for prostatic cancer cell metastasis? (kcl.ac.uk)
Interlinked actin filaments span the body of the cell and contribute to a cell's stiffness. (iciam2023.org)
Compared with actin filaments, microtubules are much more rigid, and almost straight in the dimensions of a single cell. (cerclefser.org)

A key factor expressed early in synapse formation is Msp300/Nesprin-1, which organises actin filaments around the new synapse. (ucl.ac.uk)
The dynamics of RACK1 levels in isolated adult SC of mice, i.e., progressively high during differentiation and low compared to proliferating conditions, and RACK1 silencing indicated that RACK1 promotes both the formation of myotubes and the accretion of nascent myotubes. (sdbonline.org)

The proposed research will directly address the role of increased y-actin expression and costamere instability in causing the skeletal muscle pathologies associated with dystrophinopathy. (grantome.com)
The role of actins in membrane remodeling and regulation of phagocytic activity. (betadesks.com)
and consequently membrane deformation.Second, we clarified BIN1's role in actin dynamics. (kth.se)
A particular attention is devoted to the study of the role of actin network geometry and size. (cerclefser.org)

LIMK2, a serine-specific kinase, was discovered as an actin dynamics regulating kinase. (bvsalud.org)
I suggest that in the context of semaphorin 3A signaling, RhoA-kinase serves as a switch to change the function of the F-actin cytoskeleton from promoting protrusive activity to generating contractile forces. (biologists.com)

Anatomy20

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Independent of myosin1

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20171

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