Regulation arrest of cells cell cycle. Medical search. Frequent questions

It specifically negatively regulates the PI3K-AKT signaling pathway to induce cell cycle arrest. (medscape.com)
In the HCC cells, activation of the NF-kappa B signaling pathway and IkB phosphorylation was significantly reduced by EIF5B silencing. (techscience.com)
Sodium butyrate induces mitophagy and apoptosis of bovine skeletal muscle satellite cells through the mammalian target of rapamycin signaling pathway. (usda.gov)
VALD-3 also regulated the Wnt/β-catenin signaling pathway in breast cancer cells, inhibiting the activation of downstream molecules. (researchsquare.com)
si-Prok1 arrests cell cycle, induces apoptotic changes, and inhibits the proliferation of ovarian granulosa cells through a mechanism related to the regulation of Pi3k/Akt signaling pathway. (ajol.info)
However, THP-1 cells adaptively adjusted to LeTx and overrode cell cycle arrest by activating the PI3K/Akt signaling pathway. (uwo.ca)

It further inhibited cell-cycle progression in the G1 phase by four different mechanisms: rapid downregulation of cyclin D1, induction of Chk2 with simultaneous downregulation of Cdc25A, induction of the Cdk-inhibitor p21 Cip/Waf and inhibition of ribonucleotide reductase activity resulting in reduced dCTP and dTTP levels. (nature.com)
Many chemotherapeutic agents explore defects in the cell cycle machinery of cancer cells to halt the cycle through mitosis inhibition. (medicalxpress.com)
Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)
Due to the role of oncogenic transcriptional activators NFĸB and STAT3 in survivin protein expression, and APE1/Ref-1 redox activity regulating their transcriptional activity, we assessed selective inhibition of APE1/Ref-1's redox function as a novel method to halt prostate cancer cell growth and survival. (oncotarget.com)
Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
The inhibition of cell growth was also demonstrated by cell cycle arrest at G2/M phase. (greenmedinfo.com)
In whole cells, Dox treatment prevented p34cdc2 kinase dephosphorylation without altering its synthesis, and this effect was due to neither down-regulation of cdc25C nor inhibition of protein-tyrosine phosphatase activity. (aspetjournals.org)
In MDA-MB-231 cells, ST caused a significant dose-dependent cell growth inhibition by 31- 63% (p ≤ 0.0001) in 48 h and 40-50% (p ≤ 0.0001) in 72 h. (biomedcentral.com)
New anticancer therapy strategies refer to the inhibition of CDK-cyclin complexes as an important target to prevent uncontrolled proliferation and induce apoptosis in cancer cells ( 2 ). (spandidos-publications.com)
In human monocytic cell lines U-937 and THP-1, LeTx induced cell cycle arrest in Go-Gi phase by rapid down-regulation of cyclin D1/D2 and checkpoint kinase 1 through MEK1 inhibition. (uwo.ca)

Involved in regulation of mitotic cell cycle. (nih.gov)
However, impaired splicing affects only a subset of human transcripts, enriched for mitotic cell cycle factors, leading to mitotic arrest. (biomedcentral.com)
Mitotic cell cycle progression is accomplished through a reproducible sequence of events, DNA replication (S phase) and mitosis (M phase) separated temporally by gaps known as G1 and G2 phases. (kegg.jp)

EIF5B (Eukaryotic Translation Initiation Factor 5B) is a conserved eukaryotic translation factor that mediates association of the 40S and 60S ribosomal subunits during eukaryotic translation initiation, and modulates the cell cycle progression by regulating the translation of upstream open reading frames (uORF)-containing mRNAs such as p27 and p21 [ 3 ]. (techscience.com)
These data suggest that EIF5B functions as an oncogene that promotes cancer cell growth, survival, and progression. (techscience.com)
Finally, GADD153 depletion significantly abrogates BRCA1 influence on cell cycle progression and cell death in response to doxorubicin treatment. (conicet.gov.ar)
Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC(Cdh1). (nih.gov)
We studied the effect of doxorubicin (Dox) on cell cycle progression and its correlation with DNA damage and cytotoxicity in p53-mutant P388 cells. (aspetjournals.org)
In untreated control P388 cells, p34cdc2 kinase localizes in the nucleus and cytoplasms, particularly in the centrosomes, and p34cdc2 kinase activity is dependent on cell cycle progression, with the enzyme activity increasing steadily from G1/S to G2/M and markedly declining thereafter. (aspetjournals.org)
In this dissertation, I explore the mechanisms that coordinate and regulate cell-cycle progression in the budding yeast, Saccharomyces cerevisiae. (duke.edu)
Increased levels of polyamines in cells are considered to be involved in cancer progression. (spandidos-publications.com)
CDKs regulate the cell's progression through the phases of the cell cycle by modulating the activity of key substrates. (kegg.jp)
The CDK4-cyclinD complex normally phosphorylates the retinoblastoma protein (Rb protein), leading to release of the E2F transcription factor and cell cycle progression. (medscape.com)

This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. (cancerindex.org)
Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. (thermofisher.com)
It inhibits cytokine production and cell proliferation/differentiation in various immune cells. (uwo.ca)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)

The expression of cyclin-dependent kinase inhibitors was profoundly affected with early activation and then repression of p21 cip1/waf1 and persistent activation of both p27 kip1 and p57 kip2 , whereas genes involved in cell survival and proliferation were suppressed. (aacrjournals.org)
Tan IIA inhibited proliferation of mouse keratinocytes in a dose- and time-dependent manner and induced apoptosis, resulting in S phase arrest accompanied by down-regulation of pCdk2 and cyclin A protein expression. (hindawi.com)
Our data demonstrate that Ganoderma lucidum inhibits cell proliferation in a dose- and time-dependent manner by the down-regulation of expression of cyclin B and Cdc2 and by the up-regulation of p21 expression. (greenmedinfo.com)
Cell cycle-dependent cytotoxicity, G2/M phase arrest, and disruption of p34cdc2/cyclin B1 activity induced by doxorubicin in synchronized P388 cells. (aspetjournals.org)
Our results suggest that anthracycline-induced cytotoxicity is cell cycle dependent and is mediated, at least in part, by disturbance of the regulation of p34cdc2/cyclin B1 complex, thus leading to G2/M phase arrest. (aspetjournals.org)
ST caused G0/G1 cell cycle arrest which was accompanied by a decrease in CDK4 and cyclin D1, and an increase in p21/Cip1and p27/Kip1 protein levels. (biomedcentral.com)
Cell-cycle events have been shown to be triggered by oscillations in the activity of cyclin dependent kinases (CDKs) when bound to cyclins. (duke.edu)
Purvalanol, a novel cyclin-dependent kinase inhibitor, is referred to as a strong apoptotic inducer which causes cell cycle arrest in various cancer cells such as prostate, breast and colon cancer cell lines. (spandidos-publications.com)
Cyclin-dependent kinases (CDKs), are members of the serine-threonine protein kinase family and are responsible for taking control of cell cycle regulation in eukaryotic cells. (spandidos-publications.com)
CDKs show their action by interacting with cyclins and different CDK-cyclin complexes regulate the cell cycle in the G1, S and G2/M phases ( 1 ). (spandidos-publications.com)
Activated Akt inhibited GSK3 and prevented proteasome-mediated cyclin D1 degradation in LeTx-intoxicated THP-1 cells. (uwo.ca)
Cyclin-CDK inhibitors (CKIs), such as p16Ink4a, p15Ink4b, p27Kip1, and p21Cip1, are involved in the negative regulation of CDK activities, thus providing a pathway through which the cell cycle is negatively regulated. (kegg.jp)
Another important class of tumor suppressor genes involved in cell cycle control and in the generation of human cancers is the cyclin-dependent kinase (CDK) inhibitors. (medscape.com)
The p16INK4A protein is a cell-cycle inhibitor that acts by inhibiting activated cyclin D:CDK4/6 complexes, which play a crucial role in the control of the cell cycle by phosphorylating Rb protein. (medscape.com)
Similarly, depletion in the STRIPAK component STRIP1 affects activation of GCKIII kinases and cell cycle disruption through elevated expression of cyclin dependent kinase inhibitors p21 and p27, enhanced levels of which lead to a protective effect from therapeutic treatments and increased proliferation. (lu.se)

A) Cell viability at 72 hours and (B) 6 days post-siCKAP5 treatment as measured by methoxynitrosulfophenyl-tetrazolium carboxanilide (XTT). (medicalxpress.com)
Values represent average % cell viability compared to cells treated with an equivalent concentration of siControl LNP. (medicalxpress.com)
BRCA1 expression mediates apoptosis, cell cycle arrest and decreased viability in response to doxorubicin treatment. (conicet.gov.ar)
This deadenylation deficiency caused an early DNA damage response in terms of nuclear p53 regulation, cell-cycle arrest, and reduced cell viability upon UV treatment. (jci.org)
Fifteen micromoles of purvalanol induced a reduction in cell viability by 20 and 35% within 24 and 48 h, respectively. (spandidos-publications.com)
Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)

Tumor suppressor genes encode proteins that normally provide negative control of cell proliferation. (medscape.com)
A focused survey of BRCA1 regulated genes in prostate carcinoma reveals that multiple regulators of genome stability and cell cycle control, including: BLM, FEN1, DDB2, H3F3B, BRCA2, CCNB2, MAD2L1 and GADD153, are direct transcriptional targets of BRCA1. (conicet.gov.ar)
Individuals with biallelic PARN mutations and PARN -depleted cells exhibited reduced RNA levels for several key genes that are associated with telomere biology, specifically TERC , DKC1 , RTEL1 , and TERF1 . (jci.org)
Expression of top 10 differentially expressed genes (DEGs) of Cluster1 in Figure 1B and classic spermatogonial stem cell (SSC) and SPG markers in adult human germ cells. (elifesciences.org)
We speculate that BRAFV600E has altered VDR gene targeting by inducing VDR translocation into the cytosol and/or its association capacity with its target molecules that usually results in the abrogation of cell proliferation, including β-catenin for its cytosolic re-distribution and ubiquitin ligase FBXW7 with which VDR corporately induce the degradation of genes involves in cell cycle arrest like c-myc. (bl.uk)
Finally, we also see strong regulation of many novel hedgehog- and patched-related genes, hormone biosynthetic genes, cell cycle genes, and other regulatory genes. (biomedcentral.com)
Up and down regulation of insulin-like ligands and novel genes with similarity to the extracellular domain of insulin/IGF receptors suggests opposing roles for several members of these large gene families. (biomedcentral.com)
We see evidence of TGFβ-mediated regulation of several other classes of regulatory genes, and we discuss possible functions of these genes in dauer formation. (biomedcentral.com)
Prevent DNA Damage - Curcumin protects against DNA damage to genes regulating cell replication by toxins, radiation, and other environmental factors. (beatcancer.org)
Normalize Cell Growth - Curcumin restores normal growth controls, reverses uncontrolled reproduction of malignant cells, blocks access of cancer cells to growth factors, turns on genes that prevent cell growth and turns off genes that promote cancer growth. (beatcancer.org)
Inactivation of tumor suppressor genes, whose products normally provide negative control of cell proliferation, contributes to malignant transformation in various cell types. (medscape.com)
Like Rb protein, many of the proteins encoded by tumor suppressor genes act at specific points in the cell cycle. (medscape.com)
TP53 activates the expression of genes involved in apoptosis, cell cycle regulation (p21), and MDM2. (medscape.com)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

The mechanisms of radioresistance are still poorly understood, despite it has been suggested that miRNAs play an important role in cell signaling pathways. (frontiersin.org)
Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
When resources are scant, C. elegans larvae arrest as long-lived dauers under the control of insulin/IGF- and TGFβ-related signaling pathways. (biomedcentral.com)
Eukaryotic cells respond to DNA damage by activating signaling pathways that promote cell cycle arrest and DNA repair. (kegg.jp)
Curcumin can help normalize disrupted biological signaling pathways, improve abnormal gene regulation, support the body's response to chemical stresses and toxic load, and reduce inflammatory processes. (beatcancer.org)

Reishi inhibits proliferation and induces programmed cell death in human prostate cancer cells. (greenmedinfo.com)
Ganoderma lucidum inhibits proliferation and induces apoptosis in human prostate cancer cells PC-3. (greenmedinfo.com)

These data show that di-GA exhibits three distinct anticancer activities: induction of apoptosis, cell-cycle arrest and disruption of cancer cell-induced lymphendothelial disintegration. (nature.com)

A striking decrease in Skp2 mRNA and protein expression and reciprocal increase in p27 protein level were observed in three PA-treated prostate cancer cells. (iiarjournals.org)
Protein activation and expression were analysed by western blotting, deoxyribonucleoside triphosphate levels by HPLC, ribonucleotide reductase activity by 14 C-cytidine incorporation into nascent DNA and cell-cycle distribution by FACS. (nature.com)
PTEN encodes a protein kinase of the same name and functions as a tumor suppressor through regulation of cell proliferation. (medscape.com)
One such protein is the cytoskeleton-associated protein 5 (CKAP5), widely expressed in a variety of cells to regulate the dynamics of microtubules in human cells. (medicalxpress.com)
This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. (cancerindex.org)
The DC-associated mutations identified affect key domains within the protein, and evaluation of patient cells revealed reduced deadenylation activity. (jci.org)
These anti-cancer effects are predominantly mediated through its negative regulation of various transcription factors, growth factors, inflammatory cytokines, protein kinases, and other oncogenic molecules. (nih.gov)
Classical proteomic techniques like 2-D gel electrophoresis and differential in-gel electrophoresis (DIGE) were used to generate 2D protein maps from stem cells treated with RA or CPX as well as from non-treated stem cells. (wjgnet.com)
Additionally, approximately 66% of melanomas harbour the BRAFV600E mutation, which represents a constitutive signal of the mitogen-activated protein kinase (MAPK) that is known to phosphorylate a range of substrates, resulting in the regulation involved in hyper-proliferation and survival of transformed cells. (bl.uk)
In vitro, we show that 1,25D3 to modestly increase the expression of E-cadherin mRNA and protein in SK-MEL-28, but remained absent in A375, a cell line that expresses relatively higher levels of EMT transcription factors including SNAI1 and ZEB1. (bl.uk)
Upon investigation on the regulation of VDR expression and function in the context of BRAFV600E vs. BRAFWT melanoma, we show for the first time that VDR mRNA and protein expression, independently, acquire dependency on BRAFV600E signalling. (bl.uk)
Cell cycle ratio and apoptosis in the two groups were determined using flow cytometry, while Pi3k/Akt signal route-linked protein levels were assayed by immunoblot method. (ajol.info)
While studying the activity of BRCA2 gene promoter in breast cancer cells, we discovered that this promoter has bi-directional activity and the product of the reverse activity (a ZAR1-like protein, we named ZAR2) silences the forward promoter at the G0/G1 phase of the cell. (biomedcentral.com)
Standard techniques like cell synchronization by serum starvation, flow cytometry, N-terminal or C-terminal FLAG epitope-tagged protein expression, immunofluorescence confocal microscopy, dual luciferase assay for promoter evaluation, and chromatin immunoprecipitation assay were employed during this study. (biomedcentral.com)
For example, the TP53 gene, located on chromosome 17, encodes a 53-kd nuclear protein that functions as a cell cycle checkpoint. (medscape.com)
The p19ARF protein, which is encoded by the same locus as p16, also leads to cell cycle arrest by inhibiting the ability of MDM2 to inactivate TP53. (medscape.com)
In this compiled work, we elaborate on the effect of protein expression alterations and their resulting effect on recurrence capability of cells. (lu.se)

In the G0/G1 growth phase ZAR2 is predominantly located inside the nucleus of the breast cells, binds to the BRCA2 promoter and inhibits the expression of BRCA2. (biomedcentral.com)

One of the treatments applied in cancer is radiotherapy (RT), a therapeutic modality that uses ionizing radiation to induce damage in unwanted cells. (frontiersin.org)
Here a DSB at the MAT locus is created by a site specific HO endonuclease, which we can induce synchronously in a large population of cells. (brandeis.edu)
The hormonally active form of vitamin D, 1,25-dihydroxyvitamin D (1,25D3), has shown to induce anti-cancerous effects through its cognate vitamin D receptor (VDR), in part through inducing the cell-cell adhesion molecule E-cadherin and inhibiting Wnt/β-catenin signalling. (bl.uk)

In this study, we investigated the cytotoxic and apoptotic effects of VALD-3, a Schiff base ligand synthesized from o-vanillin derivatives, on human breast cancer cells and the possible underlying mechanisms. (researchsquare.com)
The aim of the present investigation was to elucidate the cellular mechanisms whereby Tanshinone IIA (Tan IIA) leads to cell cycle arrest and apoptosis in vitro in keratinocytes, the target cells in psoriasis. (hindawi.com)
consequently cells have evolved a variety of mechanisms to repair double-strand breaks (DSBs). (brandeis.edu)
Thus, Ganoderma lucidum exerts its effect on cancer cells by multiple mechanisms and may have potential therapeutic use for the prevention and treatment of cancer. (greenmedinfo.com)
Understanding the regulatory mechanisms that control cell-cycle events remains to be a fundamental question in cell biology. (duke.edu)
The notion could be that unique cellular mechanisms are triggered in the breast cancer cells to stimulate BRCA2 gene expression as a temporary measure to regulate the growth of the breast cancer cells. (biomedcentral.com)
While BRCA2 expression is involved in cell cycle checkpoints and DNA repair, the mechanisms of cell cycle-dependent regulation of BRCA2 gene expression remains elusive. (biomedcentral.com)
In this study, we explore mechanisms in breast cancer cells that potentially lead to recurrence. (lu.se)

In this study, we further investigated the mechanism of PA-induced p27 expression in prostate cancer cells (LNCaP, androgen-independent LNCaP [AIDL] and PC-3). (iiarjournals.org)
Our results suggest that PA attenuated Skp2 expression, thereby inhibiting ubiquitination and promoting p27 accumulation in all three prostate cancer cell lines. (iiarjournals.org)
p53 is a transcription factor whose expression is increased by DNA damage and blocks cell division at the G1 phase of the cell cycle to allow DNA repair. (medscape.com)
We show here that expression of EBF3 resulted in cell cycle arrest and apoptosis. (aacrjournals.org)
In the present study, we investigated the expression profile and prognostic potential of EIF5B in HCC tissues and cell lines. (techscience.com)
Expression of cell cycle inhibitors, P21 and P27, and activation of apoptosis executer enzyme, caspase-3, were also induced by TCEE. (hindawi.com)
The expression of pro-apoptotic proteins and anti-apoptotic proteins in MCF-7 and MDA-MB-231 cells was investigated by Western blotting. (researchsquare.com)
We have previously demonstrated that Ganoderma lucidum down-regulated the expression of NF-kappaB-regulated urokinase plasminogen activator (uPA) and uPA receptor (uPAR), which resulted in suppression of cell migration of highly invasive human breast and prostate cancer cells. (greenmedinfo.com)
Furthermore, Ganoderma lucidum induced apoptosis of PC-3 cells with a slight decrease in the expression of NF-kappaB-regulated Bcl-2 and Bcl-xl. (greenmedinfo.com)
Feature plot showing the expression patterns of FOXC2 and MKI67 in human germ cells. (elifesciences.org)
I ) The developmental trajectory of the human germ cells, colored by germ cell type, FOXC2 expression cells (red), or MKI67 expression cells (red). (elifesciences.org)
F ) Heatmap showing the expression pattern of markers for cell cycle phase in different clusters. (elifesciences.org)
C ) Feature plots showing the expression pattern of classic SSCs and SPG markers in adult human germ cells. (elifesciences.org)
Similar alterations within the VDR signalling pathway are known to occur in the context of colon cancer, in which a diminished response of 1,25D3 signal is associated with increased expression of the epithelial mesenchymal transcription factor (EMT) SNAIL1, which can directly inhibit the activities of VDR and E-cadherin, resulting in epithelial cells to lose their polarity. (bl.uk)
A knockdown of SNAI1 and, independently of ZEB1, in A375 cells is insufficient to recover E-cadherin expression. (bl.uk)
We also postulate whether FBXW7, similar to the impaired regulation of VDR expression by BRAFV600E, has become altered in which the ubiquitin ligase now targets VDR for proteasomal degradation when MAPK activity is inhibited. (bl.uk)
The second model proposes that a transcription factor (TF) network oscillator controls the timing of cell-cycle events, via proper timing of gene expression, including cyclins. (duke.edu)
By measuring global gene expression dynamics in cells with persistent CDK activity, I show that periodic transcription continues. (duke.edu)
HCT 116 colon cancer cells were exposed to purvalanol, which activated ER stress via upregulation of PERK, IRE1α gene expression, eIF-2α phosphorylation and ATF-6 cleavage at early time-points in the HCT 116 colon cancer cells. (spandidos-publications.com)
BRCA2 gene expression is tightly regulated during the cell cycle in human breast cells. (biomedcentral.com)
The expression of BRCA2 gene is silenced at the G0/G1 phase of cell growth and is de-silenced at the S/G2 phase. (biomedcentral.com)
Subcellular location of ZAR2 and its expression from the reverse promoter of the BRCA2 gene are stringently regulated in a cell cycle dependent manner. (biomedcentral.com)
ZAR2 binds to BRCA2/ZAR2 bi-directional promoter in vivo and is responsible, at least in part, for the silencing of BRCA2 gene expression in the G0/G1 phase in human breast cells. (biomedcentral.com)
BRCA2 gene expression is stringently regulated during the cell cycle. (biomedcentral.com)
Specifically, a variety of other cellular populations have been identified that possess phenotypic characteristics similar to Langerhans cells, including expression of CD207 and Birbeck granules. (medscape.com)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

At a fundamental level, cancer is caused by an accumulation of genetic changes that result in unregulated cell growth and proliferation. (medscape.com)
Natural polyamines, putrescine, spermidine and spermine, play essential roles in the regulation of cell growth and proliferation. (spandidos-publications.com)

In the glioma cells, EIF5B promotes cell survival by enhancing the translation of several IRES-containing mRNAs including those encoding anti-apoptotic proteins such as XIAP and Bcl-xL [ 4 ]. (techscience.com)
These results suggest prominent in vitro anti-proliferative and pro-apoptotic effects of ST in MDA-MB-231 cells. (biomedcentral.com)
In the present study, our aim was to determine the time-dependent, ER-mediated apoptotic and autophagy induction of purvalanol in HCT 116 colon cancer cells. (spandidos-publications.com)
Moreover, we determined that during purvalanol-mediated ER stress, autophagic machinery was also activated prior to apoptotic cell death finalization. (spandidos-publications.com)
Olomoucine, roscovitine and purvalanol are examples of CDK inhibitors (CDKIs) designed and investigated for their apoptotic potential on cancer cells ( 3 ). (spandidos-publications.com)
Τheir impact on the apoptotic cell death mechanism requires further elucidation. (spandidos-publications.com)
Although purvalanol-induced cell cycle arrest and apoptotic cell death were demonstrated in prostate ( 5 ), breast ( 6 ) and colon cancer cells ( 7 ), the exact molecular mechanism of purvanol-induced apoptosis has not been elucidated yet. (spandidos-publications.com)
In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)

In addition, it can stimulate apoptosis of cells containing damaged DNA. (medscape.com)
The TP53 gene is also capable of stimulating apoptosis of cells containing damaged DNA. (medscape.com)

The single-cell transcriptome and chromatin accessibility datasets of peripheral blood mononuclear cells in Chinese Holstein cattle. (usda.gov)
Single-cell transcriptomic and chromatin accessibility analyses of dairy cattle peripheral blood mononuclear cells and their responses to lipopolysaccharide. (usda.gov)
In the present study, I demonstrated that a brief exposure to LeTx caused a continual MEK1 cleavage and prevented TNF-ce production in non-proliferating cells such as human peripheral blood mononuclear cells or mouse primary peritoneal macrophages. (uwo.ca)

In addition cells have evolved a damage-sensing checkpoint system whereby the cells delay entry into mitosis until the break has been repaired. (brandeis.edu)
Yph1p was also found in complex with a variety of other proteins, including those involved in ribosome biogenesis, cell cycle regulation, and checkpoint control. (rupress.org)

In this work, Chatterjee and the team screened the effect of CKAP5 silencing in solid cancer cell lines and in normal non-cancer epithelial cell lines as a negative control. (medicalxpress.com)
Furthermore, EIF5B knockdown suppressed epithelial-mesenchymal transition (EMT) and the cancer stem cell (CSC) phenotype. (techscience.com)
Epithelial-mesenchymal transition (EMT) is a dynamic process involved in cancer metastasis wherein cancer cells with epithelial characteristics acquire mesenchymal characteristics such as increased motility, invasion, and survival [ 6 ]. (techscience.com)
Here, we evaluated the efficacy of ST on three different human cancer cell lines including skin epidermoid carcinoma A431 cells, lung epithelial carcinoma A549 cells and breast adenocarcinoma MDA-MB-231. (biomedcentral.com)

In this study, by comparing the activity of normal cell lines and cancer cell lines after treating with resveratrol, it was found that resveratrol has more significant cytotoxicity in cancer cell lines. (mdpi.com)
Furthermore, the combined drug effects of TCEE with conventional chemotherapy agents, cisplatin and doxorubicin, were also analyzed to clarify whether TCEE enhances or antagonizes the cytotoxicity of the selected chemotherapy agents in hepatocellular carcinoma cells. (hindawi.com)

The cell-free media from MWCNT-polarized macrophages induced the migration of neutrophilic cells (differentiated from HL-60), which was blocked by Acebilustat, a specific leukotriene A4 hydrolase inhibitor, or LY239111, an LTB4 receptor antagonist, but not NS-398, a cyclooxygenase 2 inhibitor, revealing LTB4 as a major mediator of neutrophil chemotaxis from MWCNT-polarized macrophages. (cdc.gov)

Activation of p21 or p16 therefore causes cell cycle arrest. (medscape.com)

Furthermore, we found that Pls3 cell-autonomously regulates cell migration by regulating actin cytoskeleton organization, and its levels are inversely proportional to neural migration speed. (nature.com)

This TCEE also demonstrates antitumor properties such as the induction of cell cycle arrest and activation of apoptosis on human colon, lung, melanoma, osteosarcoma, and pancreatic cancer cells [ 16 - 19 ]. (hindawi.com)
Therefore, based on clinical experience as well as cytotoxic activity against multiple human cancer cells, we hypothesized that induction of apoptosis is the underlying mechanism for the treatment of psoriasis. (hindawi.com)
Further, cell death effect of ST was associated with induction of apoptosis. (biomedcentral.com)

pRB functions as a negative regulatory transcription factor during the G1 to S phase cell cycle transition. (medscape.com)
However, several studies have shown that some cell-cycle events, such as periodic transcription, can continue in the absence of CDK activity. (duke.edu)
How are periodic transcription and other cell-cycle events coupled to each other during a wild-type cell cycle? (duke.edu)
One model hypothesizes that oscillations in CDK activity controls the timing of cell-cycle events, including periodic transcription. (duke.edu)
Further, I show that during a wild-type cell cycle, checkpoints are responsible for arresting the bulk of periodic transcription. (duke.edu)
Finally, we found that CRISPR-mediated activation of Cul3 transcription fully rescues neural cell migration defects. (nature.com)

The aims of this study are to clarify the antitumor effects of TCEE on human hepatocellular carcinoma cells and also evaluate the combination drug effects with conventional chemotherapy agents, cisplatin and doxorubicin. (hindawi.com)
In contrast, the antitumor effects and related biological mechanism of TCEE as well as the combination drug effects with conventional chemotherapy agents remain unclear particularly in human hepatocellular carcinoma cells. (hindawi.com)
The aims of this preclinical study are to evaluate the capability of TCEE to suppress human hepatocellular carcinoma cells and clarify the related antitumor effects. (hindawi.com)

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-test was used to observe the proliferation of human breast cancer MCF-7 and MDA-MB-231 cells induced on VALD-3. (researchsquare.com)
Flow cytometry analysis showed that VALD-3 triggered cell cycle arrest and induced apoptosis of breast cancer cells. (researchsquare.com)
By xenografting human breast cancer cells into nude mice, we found that VALD-3 significantly suppressed tumor cell growth while showing low toxicity against major organs. (researchsquare.com)
This study is the first to show that VALD-3 induces apoptosis and cell cycle arrest in human breast cancer cells by suppressing Wnt/β-catenin signaling, indicating that it could be a potential drug for the treatment of breast cancer. (researchsquare.com)
While exploring the molecular changes associated with strong ST efficacy in breast cancer cells, we observed that ST induced cell cycle arrest as well as cell death. (biomedcentral.com)
This study provides valuable insight into the chemopreventive efficacy and associated molecular alterations of ST in breast cancer cells whereas it had only moderate efficacy on lung cancer cells and did not show any considerable effect on skin cancer cells. (biomedcentral.com)

Apoptosis was measured by Hoechst 33258/propidium iodide double staining of nuclear chromatin and the formation of gaps into the lymphendothelial barrier in a three-dimensional co-culture model consisting of MCF-7 tumour cell spheroids and human lymphendothelial monolayers. (nature.com)
Thus, the secretion of gall wasps stimulates plant cell growth and overrules homeostasis of the affected leaf area - this is similar to tumour outgrowth. (nature.com)
Brg1 is also involved in cell growth arrest, senescence and tumour supression. (thermofisher.com)
The tumour microenvironment (TME) contains several other cell types apart from cancer cells which play a role not only in the regulation of the environment but in response to treatments. (lu.se)

Among the proteins identified was Yph1p, a homologue of zebrafish pescadillo, mutations in which lead to cell proliferation defects. (rupress.org)
The mutations of BRCA2 gene predispose the cells towards neoplastic development. (biomedcentral.com)
Hermann Joseph Muller conducted three experiments in 1926 and 1927 that demonstrated that exposure to x-rays, a form of high-energy radiation, can cause genetic mutations, changes to an organism's genome, particularly in egg and sperm cells. (asu.edu)

Cy5 is labeled as magenta, and the cell membrane is stained with rabbit anti-human epidermal growth factor receptor and goat anti rabbit488 antibody. (medicalxpress.com)
In 1868, Paul Langerhans discovered the epidermal dendritic cells that now bear his name. (medscape.com)
Although the epidermal Langerhans cell has been presumed to be the cell of origin in LCH, recent studies have called this belief into question. (medscape.com)
Therefore, in addition to epidermal Langerhans cells, other potential cellular origins for LCH include dermal langerin + dendritic cells, lymphoid tissue-resident langerin + dendritic cells, and monocytes that can be induced by local environmental stimuli to acquire a Langerhans cell phenotype. (medscape.com)
Notably, LCH cells have been found to express markers of both resting epidermal Langerhans cells (CD1a, intracellular major histocompatibility complex II [MHCII], Birbeck granules) and activated Langerhans cells (including CD54 and CD58). (medscape.com)
[ 3 ] Taken together, these findings have led some to speculate that LCH is not a specific disease of epidermal Langerhans cells, but rather one of mononuclear phagocyte dysregulation. (medscape.com)

It also abrogates proliferation of cancer cells by arresting them at different phases of the cell cycle and/or by inducing their apoptosis. (nih.gov)

Cell cycle adaptations of embryonic stem cells. (nih.gov)
Murine embryonic stem cells (ESCs) and multipotent adult germline stem cells (maGSCs) were treated with CPX, which has been shown to have an antiproliferative effect on stem cells, and compared to stem cells treated with retinoic acid (RA), which is known to have a differentiating effect on stem cells. (wjgnet.com)

Langerhans cell histiocytosis (LCH) is a group of idiopathic disorders characterized by the presence of cells with characteristics similar to bone marrow-derived Langerhans cells juxtaposed against a backdrop of hematopoietic cells, including T-cells, macrophages, and eosinophils. (medscape.com)

Furthermore, Tan IIA-induced apoptosis and mitochondrial membrane potential changes were also further demonstrated by DNA fragmentation, single-cell gel electrophoresis assay (SCGE), and flow cytometry methods. (hindawi.com)
Mitochondrial regulation of apoptosis further downstream was investigated, showing changes in the mitochondrial membrane potential, cytochrome c release into the cytoplasm, and enhanced activation of cleaved caspase-3 and Poly ADP-ribose polymerase (PARP). (hindawi.com)

This study demonstrated that modulation of PI3K/Akt/GSK3 signaling cascades can be beneficial for protecting or facilitating recovery from cellular LeTx intoxication in cells that depend on basal MEK1 activity for proliferation. (uwo.ca)
The term Langerhans cell histiocytosis is generally preferred to the older term, histiocytosis X. This newer name emphasizes the histogenesis of the condition by specifying the type of lesional cell and removes the connotation of the unknown ("X") because its cellular basis has now been clarified. (medscape.com)

however, purvalanol triggered autophagy, which functions as a cell survival mechanism at early time-points. (spandidos-publications.com)
Development is arrested after the second larval molt, and the third-stage larva that is formed is structurally and behaviorally specialized for dispersal and long-term survival. (biomedcentral.com)
Arguments supporting the reactive nature of LCH include the occurrence of spontaneous remissions, the extensive elaboration of multiple cytokines by dendritic cells and T-cells (the so-called cytokine storm) in LCH lesions, and the good survival rate in patients without organ dysfunction. (medscape.com)

These findings define a novel transcriptional pathway through which BRCA1 orchestrates cell fate decisions in response to genotoxic insults, and suggest that BRCA1 status should be considered for new chemotherapeutic treatment strategies in prostate cancer. (conicet.gov.ar)

and 4) cells with features of both kerationcytes and Langerhans' cells appeared. (researchgate.net)
The epidermis is derived primarily from surface ectoderm but is colonized by pigment-containing melanocytes of neural crest origin, antigen-processing Langerhans cells of bone marrow origin, and pressure-sensing Merkel cells of neural crest origin. (medscape.com)
The ultrastructural hallmark of the Langerhans cell, the Birbeck granule, was described a century later. (medscape.com)
As a result, the pathologic cells of LCH have been hypothesized to represent Langerhans cells in a state of arrested maturation. (medscape.com)
The pathogenesis of Langerhans cell histiocytosis (LCH) is unknown. (medscape.com)

and negative regulation of meiotic nuclear division. (nih.gov)
Its anti-cancer effects are predominantly mediated through its negative regulation of tumor growth factors, inflammatory cytokines, and other oncogenic molecules. (beatcancer.org)

Down-regulation of EIF5B significantly decreased proliferation and invasiveness of the HCC cells. (techscience.com)
CD95 counts in the 3 types of white blood cells were significantly higher in PEM infants and showed improvement after nutritional rehabilitation yet not reaching the control values. (who.int)

We have demonstrated that phenylacetate(PA)-induced cell cycle arrest in human prostate cancer is mediated by increase of p27. (iiarjournals.org)
Xenograft studies using human prostate carcinoma PC3 cells demonstrate that BRCA1 depletion results in increased tumor growth. (conicet.gov.ar)
The ethanolic extract of T. camphoratus (TCEE) which contains abundant bioactive compounds including triterpenoids and polysaccharides also has antitumor effects in various human cancer cell lines. (hindawi.com)
Moreover, treatment with TCEE is found to enhance the cytotoxic effects of amphotericin B in human colon cancer cell both in vitro and in vivo [ 17 ]. (hindawi.com)
We investigated the effects of various concentrations of Tan IIA (5-10 μ g/mL) on mouse keratinocytes and human HaCat cells in vitro to confirm this hypothesis. (hindawi.com)
In this study, we investigated the effects of Ganoderma lucidum on cell proliferation, cell cycle, and apoptosis in human prostate cancer cells PC-3. (greenmedinfo.com)
Identification of the FOXC2 + spermatogonial stem cells (SSCs) in adult mouse and human testis. (elifesciences.org)
H ) t-SNE plot of germ cells in adult human testis (GSE112013), colored by germ cell type. (elifesciences.org)

Mammalian Emi2 mediates cytostatic arrest and transduces the signal for meiotic exit via Cdc20. (nih.gov)

Stimulate Apoptosis - Curcumin encourages programmed cancer cell death. (beatcancer.org)

Whereas similar ST treatments moderately inhibited the growth of A549 cells by up to 13% (p ≤ 0.05) in 48 h and 14% (p ≤ 0.05-0.0001) in 72 h. (biomedcentral.com)

Enhance Immune Surveillance - Curcumin prevents cancer cells from evading normal immune system detection and improves the body's ability to detect and destroy malignant cells. (beatcancer.org)

In vitro ubiquitination assay showed a decreased p27 ubquitination in PA-treated prostate cancer cells. (iiarjournals.org)
The challenge therefore is to identify molecular targets associated with mitosis of cancer cells. (medicalxpress.com)
A subset of cancer cells undergoing EMT acquire characteristics of stem cells and are designated as cancer stem cells (CSCs) [ 8 ]. (techscience.com)
However, the mechanism(s) responsible for the effects of Ganoderma lucidum on cancer cells remain to be elucidated. (greenmedinfo.com)
Cell growth assay, cell cycle analysis, FACS, JC-1 staining, annexin V staining and immunoblotting were used to study the efficacy of ST on cancer cells. (biomedcentral.com)
Curcumin can help protect against cancer initiation, slow its growth when it occurs, and even sensitize cancer cells to treatment. (beatcancer.org)
Reduce Production of Adhesion Molecules - Curcumin keeps malignant tissue from sticking to vessel walls and reduces cancer cells' ability to form distant metastases. (beatcancer.org)

We have shown that this regulation involves the action of a small Recombination Enhancer (RE) sequence that enables a donor on the left chromosome arm to recombine preferentially in MATa cells. (brandeis.edu)

This single cell layer of keratinocytes is attached to the basement membrane via hemidesmosomes. (medscape.com)

Precise activation and inactivation of CDKs at specific points in the cell cycle are required for orderly cell division. (kegg.jp)

In the experiments, Gurdon conducted nuclear transplantation, or cloning, of differentiated cells, or cells that have already specialized to become one cell type or another, in tadpoles. (asu.edu)

Con el fin de contrarrestar los daños causados por Aphis gossypii en las plantaciones de Fernaldia pandurata, se realizaron pruebas in vivo en las instalaciones del Laboratorio de Control de Calidad de la Universidad Salvadoreña "Alberto Masferrer" para determinar la concentración a la que se presenta. (bvsalud.org)

Cell mitosis is an attractive target to effectively treat a variety of cancer forms, where microtubule-associated proteins (MAPs) assist cells to maintain the stability of cell dynamics. (medicalxpress.com)
Both of these altered proteins lead to the eventual ability of cancer cell recurrence. (lu.se)

However, critical questions remain regarding the regulation of this developmental event. (biomedcentral.com)
In 1962 researcher John Bertrand Gurdon at the University of Oxford in Oxford, England, conducted a series of experiments on the developmental capacity of nuclei taken from intestinal epithelium cells of feeding tadpoles. (asu.edu)
Frontiers in cell and developmental biology. (lu.se)

Purvalanol is a purine-derived CDKI that binds with a high selectivity and competitively to the ATP binding side of CDK1/2 and leads to G2/M cell cycle arrest ( 4 ). (spandidos-publications.com)

Anatomy25

Organisms13

Diseases4

Chemicals and Drugs113

Analytical, Diagnostic and Therapeutic Techniques and Equipment21

Phenomena and Processes82

Disciplines and Occupations1

Information Science3

Health Care2

Signaling pathway6

Inhibition10

Mitotic cell cycle3

Progression10

Differentiation4

Cyclin15

Viability6

Genes14

Pathways6

Proliferation and induces2

Disruption1

Protein17

Inhibits1

Induce3

Mechanisms8

Expression27

Growth and proliferation2

Apoptotic8

Apoptosis of cells contain2

Peripheral blood mononuclear cells3

Checkpoint2

Epithelial4

Cytotoxicity2

Inhibitor1

Causes cell cycle arrest1

Regulates1

Induction3

Transcription6

Hepatocellular carcinoma cells3

Breast Cancer6

Tumour4

Mutations3

Epidermal6

Phases1

Embryonic2

Macrophages1

Mitochondrial2

Cellular2

Survival3

Transcriptional1

Langerhans5

Negative regulation2

Significantly2

Human8

Mediates1

Stimulate apoptosis1

0.051

Immune1

Cancer cells7

Preferentially1

Keratinocytes1

CDKs1

Nuclear1

Vivo1

Proteins2

Developmental3

Binds1