• MicroRNA (miRNA) is a short and endogenous RNA molecule that regulates posttranscriptional gene expression. (edu.hk)
  • MicroRNAs (miRNAs) are endogenous noncoding RNAs that act as post-transcriptional regulators of gene expression by binding to the 3′-untranslated region (UTR) of target mRNAs. (nature.com)
  • In most cases, circRNAs usually act as a competitive endogenous RNA (ceRNA) that can absorb miRNAs to regulate the expression of targeted genes [ 9 ]. (hindawi.com)
  • MicroRNAs (miRNAs), a family of endogenous, small non-coding RNAs (20-25 nucleotides in length), are important regulators in a variety of biological processes, including cell development, infection, immunity, and carcinogenesis, through post-transcriptional regulation of mRNA expression. (revoluciondelosgladiolos.org)
  • To further establish the role of MTp53 in regulating microRNAs expression, we will knockdown endogenous MTp53 in lung, breast and colorectal cancer cell lines and perform deep sequencing. (thegomap.org)
  • Direct neuronal conversion of resident glial cells is advantageous since they are ubiquitously distributed brain cells able to self-renew and replenish their number, making them ideal candidates for endogenous repair. (lu.se)
  • The expressions of candidate miRNAs and mRNAs were determined by real-time quantitative PCR(RT-qPCR) in MC3T3-E1 cells. (frontiersin.org)
  • microRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression, predominantly through imperfect base pairing with the 3′-untranslated region of target mRNAs ( 5 ). (spandidos-publications.com)
  • MiRNAs are small non-coding RNAs which regulate gene expression by binding to complementary sequences in mRNAs. (duke.edu)
  • microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
  • The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (cancerindex.org)
  • miRNAs exert their effects by suppressing the translation and often inducing the degradation of their target mRNAs. (nature.com)
  • Exosome contain proteins, miRNAs, and mRNAs, and the exosomal lipid bilayer protects this genetic information from degradation. (biomedcentral.com)
  • Since the mechanism of transcription of microRNA genes is similar to mRNAs, we hypothesize that in cancer cells, MTp53 alters the abundance of a subset of cellular microRNAs by regulating the activity of the above-mentioned transcription factors. (thegomap.org)
  • Candidate K-box target mRNAs were phenotypically validated using the GAL4-UAS system and live confocal microscopy to analyze dendritic morphology. (gmu.edu)
  • MicroRNAs (miRNAs) are small noncoding RNAs (~22 nucleotides in length) that regulate gene expression post-transcriptionally by imperfect binding to the 3′ untranslated region of target mRNAs in a wide variety of cell types. (biomedcentral.com)
  • MicroRNAs (miRNAs) are small, noncoding RNAs that bind to the 3' UTR of target mRNAs, and silence gene expression by inducing degradation of target mRNAs or inhibition of protein translation [ 10 ]. (cdc.gov)
  • MicroRNAs (miRNAs) are a class of small noncoding RNA molecules that negatively regulate gene expression by inducing target mRNA cleavage or by inhibiting protein translation. (friendsofstalphonsus.org)
  • Multiple miRNA-mRNA prediction databases were searched by differentially expressed genes (DEGs) to obtain pairs of a miRNA-DEG regulatory network. (frontiersin.org)
  • It regulates Sp7 protein expression and induces expression of major bone matrix protein genes, such as Col1a1, Spp1, Ibsp, Bglap2, and Fn1 ( 7 , 8 ). (frontiersin.org)
  • MicroRNAs function to regulate the expression levels of other genes by several mechanisms. (wikipedia.org)
  • Therefore, it is important to describe their mechanisms of actions, expression patterns and possible target genes and cellular pathways with which they interact. (mdpi.com)
  • This Special Issue of "Genes" seeks reviews and original papers covering a wide range of topics related to microRNA biology, such as regulation of expression in various disorders (cancer, metabolism, autoimmunity to mention but a few), genetics of microRNAs and their target sites, functional analysis of microRNA function and studies of interactions between microRNAs and target genes. (mdpi.com)
  • Investigation of hub genes for the development of potential therapeutic targets and candidate biomarkers is warranted. (nature.com)
  • The immune-related DEOSGs and hub genes were identified by weighted gene co-expression network analysis (WGCNA) and protein-protein interaction (PPI) analysis, respectively. (nature.com)
  • The mRNA expression of diagnostic genes was determined by qRT-PCR analysis. (nature.com)
  • Finally, we constructed the drug, transcription factors (TFs), and microRNA network of the diagnostic genes. (nature.com)
  • Background: The study purpose was to characterize the mycobiome and its associations with the expression of pathogenic genes in esophageal squamous cell carcinoma (ESCC). (bvsalud.org)
  • This study aimed to evaluate the expression levels of miR-34a and 11 of its bioinformatically selected target genes and proteins to test their potential dysregulation in RCC. (hindawi.com)
  • Over the past few years, emerging numerous bioinformatic tools have been developed to identify candidate disease-causing genes [ 4 ], including microRNA (miRNA) genes. (hindawi.com)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • There is evidence that clearly demonstrates that, apart from genetic and epigenetic abnormalities, the dysregulation of miRNAs may also contribute to the aberrant activation of oncogenes and the inactivation of tumor suppressor genes in human carcinogenesis ( 9 , 10 ). (spandidos-publications.com)
  • To identify pro-apoptotic genes targeted by the five anti-apoptotic miRNAs, I validated one previously published target and identified nine novel targets by performing photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) in the EBV+ NPC cell line C666. (duke.edu)
  • Next, I thoroughly demonstrated that the 10 candidate target genes were substantially suppressed by expression of the relevant miR-BARTs, as measured by 3'UTR-containing firefly luciferase (FLuc) expression, mRNA and protein levels, and knockdown of seven of the 10 candidate genes could suppress apoptosis, mimicking the effects of relevant miR-BARTs. (duke.edu)
  • Our objective is to identify the related miRNAs and their associations with genes frequently involved in CRC microsatellite instability (MSI) and chromosomal instability (CIN) signaling pathways. (edu.hk)
  • A regression model was adopted to identify the significantly associated miRNAs targeting a set of candidate genes frequently involved in colorectal cancer MSI and CIN pathways. (edu.hk)
  • We also highlight some recent studies on several of the miR-128 targets which should be investigated further as potential candidate genes for therapeutic interventions. (um.edu.my)
  • Each microRNA can regulate the expression of hundreds of genes by blocking mRNA translation and/or inducing mRNA decay. (thegomap.org)
  • MicroRNA genes reside in regions of the genome as distinct transcriptional units as well as in clusters of polycistronic units. (thegomap.org)
  • RNA polymerase II transcribes microRNA genes, generating long primary transcripts. (thegomap.org)
  • Among the most pressing questions regarding this unusual class of regulatory microRNA-encoding genes is how their expression is regulated. (thegomap.org)
  • Having identified the common set of microRNAs regulated by MTp53, we will next investigate (1) whether candidate microRNAs are also regulated by other aggressive mutants of p53 (such as R175H) (2) changes in transcription and regulation of candidate microRNA genes by MTp53 and MTp53-interacting transcription factors such as E2F1, ETS1 and p63/p73. (thegomap.org)
  • We have previously demonstrated that the K box miRNAs, including miR-2b/miR-13b regulate the expression of genes required to restrict dendritic branching. (gmu.edu)
  • To further quantitatively assess miR-based regulation of putative target genes that produced significant increases in dendritic branching morphology, we conducted target mRNA expression validation studies and confirmed that these K box miRNAs regulate the expression of noc, Tab2, spas and CG4911 in modulating class-specific dendritic homeostasis. (gmu.edu)
  • In contrast to the K box miRNAs, which regulate the expression of genes required to restrict dendritic branching, we identified miR-279 as one miRNA that functions by regulating the expression of genes required to promote dendritic growth and branching via gain-of-function analyses. (gmu.edu)
  • There were 29 MADS-box genes, as well as a large number of floral-related regulators and hormone-responsive genes, considered as candidates regulating floral patterning of C. goeringii . (biomedcentral.com)
  • Small RNA sequencing revealed 132 conserved miRNA families expressed in flowers of C. goeringii , and 11 miRNAs corresponding to 455 putative target genes were considered to be responsible for multi-tepal development. (biomedcentral.com)
  • These novel findings were confirmed by real-time polymerase chain reaction analysis: higher expression of HAND genes correlated with lower expression of miR-363, miR-367, miR-181a and miR-181c. (biomedcentral.com)
  • From the list of genes that were targeted by the 73 diagnostic miRNAs, DGKE and WDR47 had significant associations with responses to both systemic therapies and radiotherapy in lung cancer. (cdc.gov)
  • Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
  • Pansensitive and panresistant genes to 21 NCCN-recommended drugs with concordant mRNA and protein expression were identified. (cdc.gov)
  • Here we examined 145 SNPs in 6 miRNA processing genes and in 78 miRNAs which target genes known to be important in breast cancer among 906 African American (AA) and 653 European American (EA) cases and controls enrolled in the Women's Circle of Health Study (WCHS). (cdc.gov)
  • We found a number of SNPs in miRNAs and processing genes in association with breast cancer overall or stratified by estrogen receptor (ER) status. (cdc.gov)
  • Because miRNAs may regulate approximately 60% of human genes [ 11 ], the relationship between miRNAs and human diseases has been extensively explored in the last decade. (cdc.gov)
  • MicroRNAs (miRNAs), single-stranded RNA molecules with 19~25 nucleotides in length, are recognized as key modulators governing multiple gene expression via binding to the 3' untranslated region (3'-UTR) of target mRNA at the posttranscriptional level [ 7 ]. (oncotarget.com)
  • Circular RNAs (circRNAs) are a form of noncoding and closed loop RNA molecules and play vital roles in the progression of various types of cancer in humans. (hindawi.com)
  • MiRNAs are short, non coding RNA molecules that regulate gene expression post transcriptionally. (um.edu.my)
  • They belong to a class of small (19-24 nt) non coding RNA molecules. (sunway.edu.my)
  • MicroRNAs constitute a pervasive post-transcriptional filter on protein or mRNA expression levels that are likely to control developmental timing, cellular differentiation, stress responses, metabolism and proliferation. (mdpi.com)
  • Further, high NEK2 expression promoted proliferation, colony formation, migration and invasion of HCC cell lines. (oncotarget.com)
  • miRNAs affect a broad range of biological functions including development, apoptosis, proliferation and differentiation ( 6 - 8 ). (spandidos-publications.com)
  • The effect of regulated expression of the miRNA on cell migration, invasion, proliferation and apoptosis was examined by wound healing, transwell assays, CCK-8 assays, colony formation and flow cytometry assays, respectively. (oncotarget.com)
  • TRIM13 inhibited cell proliferation and induced cell apoptosis by regulating NF-κB pathway in non-small-cell lung carcinoma cells. (cancerindex.org)
  • In our preliminary experiments to examine the effect of those 4 miRNAs on proliferation function of ESCC cell lines, we found that miR-101 or miR-143 could inhibit the proliferation of ESCC cell lines, but miR-26a or miR-144 alone did not. (revoluciondelosgladiolos.org)
  • In addition, we have reported that miR-101 inhibits ESCC proliferation and metastasis by regulating COX2 [41]. (revoluciondelosgladiolos.org)
  • Recent studies have shown that microRNAs regulate several cancer-related biological processes, including enhanced proliferation, tumor angiogenesis and metastasis. (thegomap.org)
  • and the miR319/TCP4-miR396/GRF regulatory cascade probably regulating cell proliferation of the multi-tepal development. (biomedcentral.com)
  • Further, we examined the putative functional implication of the most differentially regulated miRNA (miR-324-3p) in differentiation, proliferation and migration of cultured keratinocytes by qRT-PCR, immunofluorescence, and scratch assay. (edu.au)
  • MicroRNAs (miRNAs) are a class of short, non-coding RNAs (~22 nucleotides) that function as critical post-transcriptional regulators of gene expression. (gmu.edu)
  • With respect to the reported regulation of this miRNA in Scrapie-infected mice, we propose that upregulation of hsa-miR-342-3p may be a general phenomenon in late stage prion disease and might be used as a novel marker for animal and human TSEs. (biomedcentral.com)
  • Thus we hypothesized that miRNA regulation may also play a role in human prion diseases. (biomedcentral.com)
  • Regulation of COX-2 expression by miRNAs has been extensively studied in a variety of human tumors, but this kind of regulation in ESCC remains unclear [30C40]. (revoluciondelosgladiolos.org)
  • The day-to-day fluctuations that most investigators focus upon are not found in this latest group, suggesting that short-term and long-term miR regulation are differentially regulated. (ca.gov)
  • MicroRNAs are an abundant class of small non-coding RNAs that play key roles in gene regulation. (thegomap.org)
  • Our findings suggest that the increased invasiveness of MTp53 cells could be partly mediated by increased levels of oncogenic microRNAs and down-regulation of tumor suppressor microRNAs. (thegomap.org)
  • miRNA-mediated gene regulation has been implicated across numerous species and in a wide variety of tissues with functional role in diverse biological processes including embryonic development, stem cell division, germline specification, neuronal morphogenesis and cancer. (gmu.edu)
  • To elucidate the molecular bases of K box miRNA-mediated regulation of dendritic development, we employed bioinformatics strategies to identify putative mRNA targets of miR-2b/miR-13b based on inverse correlation expression analyses. (gmu.edu)
  • miRNAs are approximately 22-nucleotide non-coding RNAs that participate in post transcriptional gene expression regulation through mRNA degradation, translational inhibition or chromatin-based silencing mechanisms. (sarcomahelp.org)
  • consequently, no major conclusions regarding the expression and regulation of miRNA in LPS could be made. (sarcomahelp.org)
  • There was consistent up-regulation of miR-1246 gene expression that targeted the DLG3 gene which contributes to neurological pathogenesis. (sunway.edu.my)
  • Conclusion: Together, we offer a platform for understanding miRNA dynamic regulation in follicular stem and progenitor cells in baldness and highlight miR-324-3p as a promising target for its treatment. (edu.au)
  • Given the notion that a single miRNA may have multiple cellular targets and given the existence of vast numbers of miRNAs (~1,500 in humans), we can expect to witness the discovery of novel miRNA-dependent regulation in the modulation of versatile biological functions [ 4 ]. (biomedcentral.com)
  • In addition, there are specific miRNAs that differ between PsA and PsO, so therefore, a shift of miRNA signature is observed in patients with PsA, and this was further in line and associated with inflammatory lesions detected by MRI. (medpagetoday.com)
  • miRNA deregulation has been identified in a variety of epithelial origin cancers, where changes in specific miRNAs expression possibly contribute to tumor growth, progression, metastasis, and chemoresistance. (sarcomahelp.org)
  • The findings may help to provide promising candidate biomarkers and therapeutic targets for AD. (nature.com)
  • Recent studies reported microRNAs as promising biomarkers for early cancer detection, accurate prognosis, and molecular targets for future treatment. (hindawi.com)
  • The aim of this study was to investigate the potential of circulating cell-free miRNAs as biomarkers of CRC, and their efficiency at delineating patients with polyps and benign adenomas from normal and cancer patient groups. (biomedcentral.com)
  • Changes of miRNAs in exosome have been reported in different disease diagnosis and provided as potential biomarkers. (biomedcentral.com)
  • Exosomal miRNA expression profiles may provide supplemental biomarkers for diagnosing and subtyping HFMD infections. (biomedcentral.com)
  • Changes in exosomal miRNAs have been reported in patients diagnosed with Alzheimer's disease (AD), and miRNAs have been shown to provide diagnostic biomarkers [ 26 ]. (biomedcentral.com)
  • How Reliable Are Gene Expression-Based and Immunohistochemical Biomarkers Assessed on a Core-Needle Biopsy? (lu.se)
  • Further validation indicated that the 4 significant differentially expressed candidate miRNAs (miR-671-5p, miR-16-5p, miR-150-3p, and miR-4281) in exosome showed the same changes as in the microarray analysis, and the expression level of three miRNAs (miR-671-5p, miR-16-5p, and miR-150-3p) were significantly different between MHFMD or ESHFMD and the healthy controls. (biomedcentral.com)
  • This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in posttranscriptional silencing of the gene expression [ 5 ]. (hindawi.com)
  • Dysregulation of miRNAs is also implicated in various diseases, including cancer. (spandidos-publications.com)
  • This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
  • Dysregulation of miRNA could possibly be an important contributing factor to LPS sarcomagenesis, especially to the process of dedifferentiation. (sarcomahelp.org)
  • Recently, miRNA-messenger RNA (mRNA) regulatory relationships have been confirmed during biological processes, including osteogenic differentiation. (frontiersin.org)
  • Upregulation of miR-488 and miR-920 could suppress MSU-induced IL-1β protein expression in THP-1 cells, but no significant difference in IL-1β messenger RNA levels was observed. (biomedcentral.com)
  • Finally, we identified 76 therapeutic agents, 152 miRNAs targets, and 91 TFs regulatory networks. (nature.com)
  • Regulatory microRNA sequences may stimulate self-renewal of these muscle cells. (natureasia.com)
  • In addition, due to their rapid and reversible regulatory capacity, miRNAs are prime candidate facilitators of responses to proteotoxic stress. (elifesciences.org)
  • miRNAs are noncoding, regulatory RNAs expressed dynamically during differentiation of hESC. (ca.gov)
  • As miRNAs play a major role in the host regulatory system, there is a huge opportunity for interplay between host miRNAs and EV-A71 expressions. (sunway.edu.my)
  • Recent evidence has shown a role for non-coding regulatory RNAs, such as microRNAs (miRNAs), in skin and hair disease. (edu.au)
  • Our results have laid down a solid foundation in exploration of novel CRC mechanisms, and identification of miRNA roles as oncomirs or tumor suppressor mirs in CRC. (edu.hk)
  • Aim 1 - Generate miR expression profiles using deep sequencing for defined stages of development from pluripotent to endocrine cells and select candidate miRs for manipulations involving silencing and overexpression. (ca.gov)
  • Aim 2 - Identify miRs targets through deep sequencing of RNA induced silencing complexes (RISC) in defined cell populations and assessment of their roles in differentiation in vitro and after experimental transplantation. (ca.gov)
  • This was valuable as it helped us determine which miRs had similar expression patterns. (ca.gov)
  • We have found that master regulators of miRs exist and/or are being regulated by another regulator but that regulator is biasing them over a large time scale (weeks). (ca.gov)
  • Methods: Normal follicular stem and progenitor cells, as well as follicular patient's stem cells, were sorted from hair follicles, and a miRNA q-PCR profiling to compare the expression of 748 miRNA (miRs) in sorted cells were performed. (edu.au)
  • This may include oncomir (oncogenic miRNA) inhibition, or tumor suppressor-miRNA replacement therapies [ 6 , 9 ]. (hindawi.com)
  • We also found that the tumor suppressor miRNA, let-7, was significantly down-regulated by mutant p53 in all the cell lines that tested. (thegomap.org)
  • Cell apoptosis and DDP-chemosensitivity was remarkably increased by knockdown of Src proto-oncogene (Src) expression, which was subsequently reversed by rescue of Src expression in miR-31-expressing cells. (oncotarget.com)
  • The impact of host miRNAs on immune activation, shutdown of host protein synthesis, apoptosis, signal transduction and viral replication are discussed. (sunway.edu.my)
  • Utilizing a Wnt signaling FLuc reporter TOPflash which measures the Wnt signaling activation, I confirmed that expression of many miR-BARTs that target Wnt signaling inhibitors can indeed upregulate the Wnt signaling pathway. (duke.edu)
  • In conclusion, our work reveals the impacts of circIFT80 as ceRNA in the progression of CRC, by which sponging miR-142, miR-568, and miR-634 enhanced the expression levels of β -catenin and activation Wnt/ β -catenin pathway. (hindawi.com)
  • Here, we report that the microRNA miR-1 regulates the autophagy pathway through conserved targeting of the orthologous T re-2/ B ub2/ C DC16 (TBC) Rab GTPase-activating proteins TBC-7 and TBC1D15 in Caenorhabditis elegans and mammalian cells, respectively. (elifesciences.org)
  • Moreover, BmCPV-miR-1 could suppress the expression of the target gene, B. mori inhibitor of nuclear factor kappa-B kinase subunit beta (BmIKKβ), via binding to the target mRNA 3′-untranslated region, which fine-tuned the host NF-κB signaling pathway and consequently enhanced viral replication. (engreen.com.cn)
  • In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)
  • This study aimed to investigate cisplatin (DDP)-susceptibility regulated by expression of the miRNAs and underlying pathways in GBC. (oncotarget.com)
  • Over the past decade, misregulated miRNA pathways have been associated with various diseases such as cancer, neurodegenerative diseases, and neurodevelopmental disorders. (um.edu.my)
  • The identification of microRNAs regulated by multiple p53 mutants in a panel of cell lines will be indicative of key pathways relevant to mutant p53-mediated tumorigenesis. (thegomap.org)
  • To investigate whether prion-induced neurodegeneration is linked to deregulation of miRNA in the brain of affected individuals, we analyzed differential miRNA expression in brains of BSE-infected non-human primates ( Macaca fascicularis ) as a model for Creutzfeldt-Jakob disease in humans. (biomedcentral.com)
  • Not much is known about the expression and deregulation of miRNA in STS generally and in LPS specifically. (sarcomahelp.org)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • The advanced pathological grade was associated with strong TGFB1, VEGFA, and Ki67 protein expression and absent Tp53 staining. (hindawi.com)
  • Previously, we reported that miRNA-101 (miR-101) is downregulated by the HBV X protein ( 20 ). (spandidos-publications.com)
  • In EBV latently infected epithelial cells, such as nasopharyngeal carcinoma (NPC) and gastric carcinoma (GaCa) cells, viral protein expression is low. (duke.edu)
  • miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (cancerindex.org)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • The RNA-binding protein FMR1 has a key role in the neurodevelopmental disorder fragile X syndrome, but the RNAs targeted by the protein were mostly unknown. (natureasia.com)
  • The finding that the SIRT6 protein suppresses tumour formation by regulating metabolism adds weight to this view. (natureasia.com)
  • We found that TRIM13 mRNA and protein expression was reduced in NSCLC tissues and cell lines in comparison to paired non-cancerous tissues and a human normal bronchial epithelial cell line, respectively. (cancerindex.org)
  • Overexpression of miR-488 and miR-920 could significantly inhibit the gene and protein expression of IL-8 and TNF-α in MSU-induced THP-1 cells. (biomedcentral.com)
  • Based on our identified miRNA-regulated molecular machinery, an inhibitor of PDK1/Akt BX-912, an anthracycline antibiotic daunorubicin, and a multi-targeted protein kinase inhibitor midostaurin were discovered as potential repositioning drugs for treating lung cancer. (cdc.gov)
  • MiRNAs are derived from primary transcripts (pri-miRNA) that are sequentially processed into their mature form by the RNase III type nucleases DROSHA and DICER [ 5 ]. (biomedcentral.com)
  • MiRNAs are short (20-22nt) non-coding RNAs that negatively regulate gene expression through either mRNA degradation or translational repression [ 5 ]. (biomedcentral.com)
  • This study indicated that the exosomal miRNA from patients with different condition of HFMD express unique miRNA profiles. (biomedcentral.com)
  • Our studies, supported by the Liddy Shriver Sarcoma Initiative, aimed to identify unique miRNA expression profiles of WDLPS and DDLPS with the hope that such signatures could provide insights into our understanding of the molecular determinants driving these two entities. (sarcomahelp.org)
  • The miRNA-mRNA interactions were verified by dual-luciferase reporter gene assays and experiments using mimics miRNA or their inhibitors. (frontiersin.org)
  • The central hypothesis driving the research is that miRNAs are essential regulators of endocrine cell development. (ca.gov)
  • To test the hypothesis that mutations in p53 can regulate transcription of microRNAs in cancer cells, we decided to use stable cell lines expressing MTp53 in p53-null H1299 lung cancer cells. (thegomap.org)
  • Our results provide new evidence supporting the hypothesis that RNA viruses could generate miRNAs to modulate antiviral host defense. (engreen.com.cn)
  • Altogether, these results suggested that systemic miR-143/miR-145 expression could support tumor growth and launched the hypothesis that these miRNAs affected the stroma rather than epithelial cells [ 4 ]. (biomedcentral.com)
  • MicroRNAs (miRNAs) are small non-coding RNAs that function as novel gene expression regulators at the post-transcriptional level. (engreen.com.cn)
  • The expression of miR‑101 in HCCs and serum was evaluated by real‑time polymerase chain reaction. (spandidos-publications.com)
  • Notably, serum miR‑101 levels were found to have an inverse correlation with tissue miR‑101 expression levels. (spandidos-publications.com)
  • The expression of serum miR‑101 in patients with HBV‑related HCC was significantly higher than that in the healthy controls, and this increase correlated with hepatitis B surface antigen positivity, HBV DNA levels and tumor size. (spandidos-publications.com)
  • Serum blood samples were analyzed for circulating miRNA. (medpagetoday.com)
  • detected miRNAs in the serum and plasma blood components of humans and other animals. (biomedcentral.com)
  • Here, we employed microarray methods to compare the miRNAs of exosome from serum samples collected from normal children and patients with mild HFMD (MHFMD) and extremely severe HFMD (ESHFMD). (biomedcentral.com)
  • Among the miRNAs up-regulated in H1299-MTp53 cells and these cell lines expressing p53shRNAs, was the oncogenic miRNA miR-155, a recently discovered target of MTp53. (thegomap.org)
  • MiRNA expression has been shown to be altered in cancerous tissue compared to normal tissue and different miRNAs have been attributed oncogenic and tumour suppressor qualities [ 6 ]. (biomedcentral.com)
  • There were 11 different miRNAs in exosomes of MHFMD and ESHFMD compared to healthy children, of which 4 were up-regulated and 7 were down-regulated. (biomedcentral.com)
  • This study demonstrates the roles of miR-488 and miR-920 in regulating the production of proinflammatory cytokines in the pathogenesis of GA. These findings suggest that miR-488 and miR-920 could serve as potential therapeutic targets in the treatment of GA. (biomedcentral.com)
  • Objectives: To investigate the roles of miRNAs affecting AGA in normal and patient's epithelial hair cells. (edu.au)
  • MicroRNAs regulate target gene expression post-transcriptionally in a myriad of cell types and play critical roles in diverse physiological and pathological processes, including cardiomyocyte development, differentiation, and regeneration. (biomedcentral.com)
  • Incorporation of miRNAs in RNA-induced silencing complexes (RISCs) then leads to decay or to translational repression of complementary mRNA targets. (biomedcentral.com)
  • miRNAs predominantly interact with mRNA targets through imperfect binding to motifs in target mRNA 3′-untranslated regions (3′UTRs) ( Bartel, 2009 ). (elifesciences.org)
  • The nature of imperfect binding specificity means that a single miRNA can regulate a large number of mRNA targets involved in complex cellular processes, thereby tightly controlling genetic networks during development and in response to stress ( Pocock, 2011 ). (elifesciences.org)
  • We searched the databases TargetScan, PicTar, miRwalk, DIANAmT, microRNA, Microcosm Targets and MicroRanda for miRNAs that might bind to the 3 -UTR of COX-2. (revoluciondelosgladiolos.org)
  • Four candidates including miR-101, miR143, miR-26a and miR-144 were found via computational prediction of microRNA targets. (revoluciondelosgladiolos.org)
  • We are working under the postulate that miRNAs are logical targets for in vitro experimentation because of their role in mediating pancreatic cell development. (ca.gov)
  • 8 The results demonstrated that miRNA expression signatures were clearly distinct among the tumor types studied, suggesting their possible role in sarcomagenesis, and their potential as diagnostic markers or even therapeutic targets. (sarcomahelp.org)
  • In contrast, miR-30a that targets calcium channels for membrane transportation was down-regulated. (sunway.edu.my)
  • miRNAs could thus be the best targets for understanding cardiac specialization during differentiation of human embryonic stem cells (hESCs). (biomedcentral.com)
  • These miRNAs which targeted β -catenin mRNA were confirmed by dual-luciferase reporter system and RNA-pulldown. (hindawi.com)
  • Then, generation and function analyses were conducted on one of the candidate miRNAs, BmCPV-miR-1, in the BmN cells and the silkworm larvae by RNA interference, quantitative PCR, dual-luciferase assay. (engreen.com.cn)
  • however, the significance of circulating miRNAs in hepatitis B virus (HBV)‑related hepatocellular carcinoma (HCC) remains largely unknown. (spandidos-publications.com)
  • Based on our prior observations that miRNA‑101 (miR‑101) is downregulated by HBV and induces epigenetic modification, we sought to test whether circulating miR‑101 may serve as a potential biomarker for HCC. (spandidos-publications.com)
  • These characteristics make circulating miRNAs good candidates for noninvasive testing for cancer. (spandidos-publications.com)
  • Circulating miRNAs have been suggested as diagnostic markers for various types of cancer ( 14 - 19 ). (spandidos-publications.com)
  • Early PsA Diagnosis: Can Circulating MicroRNA Patterns Help? (medpagetoday.com)
  • Realizing the importance of earlier diagnosis of psoriatic arthritis (PsA) in the quest for preventing joint damage leading to decreased function and permanent structural abnormalities, Dr. Haschka and colleagues looked for specific circulating miRNA signatures that are associated with PsO and PsA to be used as molecular markers. (medpagetoday.com)
  • Their study, "Identification of circulating microRNA patterns in patients in psoriasis and psoriatic arthritis," was published online in Rheumatology (Oxford) in February 2023. (medpagetoday.com)
  • Circulating miRNAs are attractive biomarker candidates as they can be easily collected, are stable under different storage conditions and can be measured using specific assays. (friendsofstalphonsus.org)
  • We identified two circulating miRNAs capable of distinguishing patient groups with different diseases of the colon from each other, and patients with advanced cancer from benign disease groups. (biomedcentral.com)
  • An MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. (frontiersin.org)
  • The microRNA-31 (miR-31) was selected by microarray due to the biggest fold change between DDP-resistant and parental cells. (oncotarget.com)
  • The microarray was used to select the candidate miRNA in two DDP-resistant GBC cell lines. (oncotarget.com)
  • A miRNA microarray was used to analyze the miRNA expression profiles in peripheral white blood cells (WBCs) of patients with GA. THP-1 cells were transfected with miRNA mimics, stimulated by MSU crystals, and then subjected to quantitative real-time polymerase chain reaction or Western blot analysis. (biomedcentral.com)
  • Different expression of miRNAs in exosomes across all the three groups were screened using miRNA microarray method. (biomedcentral.com)
  • In a recent study using a microarray approach, 27 STS of seven different histological subtypes were profiled for miRNA expression. (sarcomahelp.org)
  • The study also showed binding of miR-181d to ANGPTL3 in an in vitro model of liver cells that results in the inhibition of ANGPTL3 expression and consequently increase LPL activity (Figure 3). (dasmaninstitute.org)
  • MicroRNA-34a gene (MIR-34A) that is located on chromosome 1p36 belongs to one of evolutionary-conserved miRNA families (MIR-34 family) that consists of three members: MIR-34A, MIR-34B, and MIR-34C [ 10 ]. (hindawi.com)
  • However, the role of microRNA in mammalian tissue development and differentiation is still quite uncharacterized. (mdpi.com)
  • Aberrant expression of miRNAs has been widely reported in human cancers with both up- and downregulation detected in HCC tumor tissue relative to the corresponding normal tissue ( 11 - 13 ). (spandidos-publications.com)
  • miRNAs are notably stable in blood, and their expression patterns appear to be tissue-specific. (spandidos-publications.com)
  • We identified three significantly associated mRNA-miRNA pairs: BCL2 was positively associated with miR-16 and SMAD4 was positively associated with miR-567 in the CRC tissue, while MSH6 was positively associated with miR-142-5p in the normal tissue. (edu.hk)
  • This has been shown by miRNA expression analyses in tumor and normal tissue samples and in gain-of-function and loss-of-function studies, both in vitro and in vivo [ 3 ]. (biomedcentral.com)
  • These miRNAs are also not degraded in these fluids as they are joined to proteins or extracellular vesicles. (medpagetoday.com)
  • Tet proteins regulate gene expression by removing methyl groups from DNA bases. (natureasia.com)
  • Recently, the potential therapeutic use of miRNAs has been evaluated due to their dynamic and reversible properties. (hindawi.com)
  • Our results demonstrated that miR-31reduced significantly in GBC cells rendering resistance to cisplatin, and upregulated expression of miR-31 augmented chemosensitivity, presenting a therapeutic potential to overcome drug resistance in GBC. (oncotarget.com)
  • This study aimed to find out more candidate miRNA-mRNA pairs involved in the osteogenic differentiation of MC3T3-E1 cells. (frontiersin.org)
  • There were 7 overlapped miRNA-mRNA pairs identified during osteogenic differentiation of MC3T3-E1 cells, including mmu-miR-204-5p-Arhgap11a, mmu-miR-211-5p-Arhgap11a, mmu-miR-24-3p-H2afx, mmu-miR-3470b-Chek2, mmu-miR-3470b-Dlgap5, mmu-miR-466b-3p-Chek1, and mmu-miR-466c-3p-Chek1. (frontiersin.org)
  • Osterix (OSX) is a transcription factor in the SP family, which is essential for osteoblast differentiation, and its expression is closely related to Runx2 ( 9 ). (frontiersin.org)
  • The long term goal of our research is to understand the biochemical processes that regulate differentiation of human embryonic stem cells (hESCs) into pancreatic progenitor cells, and ultimately, glucose-responsive, insulin producing (beta) β cells. (ca.gov)
  • Of the several genetic factors that contribute to stem cells differentiation, miRNAs (microRNAs) are emerging as important determinants. (ca.gov)
  • Mapping developmental expression of miRNAs during transition from pluripotency to pancreatic progenitors will help clarify the mechanisms underlying lineage specification and ultimately enhance differentiation protocols. (ca.gov)
  • We have made a cell line that reports the expression of PDX1, a marker for both pancreatic precursors and for mature beta cells, in order to select and purify the target cells in late differentiation. (ca.gov)
  • B) Deep sequence purified hESC populations from selected time points during hESC differentiation and develop algorithms to analyze change in miRNA expression in complex systems. (ca.gov)
  • To this end, the authors performed miRNA expression profiling in undifferentiated hESCs and CMs at day 8 and day 14 after differentiation. (biomedcentral.com)
  • MicroRNAs (miRNAs) are small noncoding RNAs regulating gene expression by mRNA targeting. (biomedcentral.com)
  • The present study explored the expression and potential role of TRIM13 in non-small-cell lung carcinoma (NSCLC). (cancerindex.org)
  • 22 candidate BmCPV-encoded miRNAs were identified in this study through small RNA sequencing, stem-loop RT-PCR and qRT-PCR. (engreen.com.cn)
  • MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at a post-transcriptional level, inhibiting mRNA translation or degrading mRNA. (biomedcentral.com)
  • Because of this local degeneration of a relatively small population of dopaminergic neurons in the midbrain, PD has been considered an especially interesting candidate for cell-replacement therapy. (lu.se)
  • In a PCR array analysis of 84 transcription factors, either overexpressing DEFB1 or siRNA silencing of DEFB1 expression significantly modulated the expression of signal transducer and activator of transcription 3 (STAT3). (cdc.gov)
  • The role of epigenetic factors such as microRNAs in regulating many biological processes associated with obesity and diabetes including lipid metabolism has been recently highlighted. (dasmaninstitute.org)
  • Additionally, miRNAs are released into the blood and other biological fluids from cells, which then allows them to be detected with minimally or non-invasive techniques. (medpagetoday.com)
  • Recently, much attention has focused on the impact of microRNAs (miRNAs) on tumorigenesis and cancer progression. (sarcomahelp.org)
  • There is no reported information about the role of miRNAs in hair epithelial cells of AGA. (edu.au)
  • Recent findings suggest these microRNAs (miRNAs) have a critical role that affects the stroma rather than epithelial cells. (biomedcentral.com)
  • Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
  • Here we generate a UAS-miRNA library of Drosophila stocks and perform a genetic screen to identify miRNAs whose overexpression in the IPCs inhibits body growth in Drosophila . (nature.com)
  • Here we report the results of a Drosophila UAS-miRNA library screen that identifies the conserved miR-9a as a regulator of insulin signalling and body growth. (nature.com)
  • Since the insulin and growth phenotypes are rescued by expression of sNPFR1, we propose that the conserved miR-9a/miR-9 regulates body growth by modulating insulin signalling through its conserved sNPFR1/NPY2R target in both Drosophila and mammals. (nature.com)
  • Drosophila melanogaster dendritic arborization (da) neurons have emerged as an exceptional model for miRNA studies. (gmu.edu)
  • m6A mRNA methylation regulates human β-cell biology in physiological states and in type 2 diabetes. (dasmaninstitute.org)
  • MicroRNAs (miRNAs) regulate many physiological processes including body growth. (nature.com)
  • We analyzed the relationship between differential expression of NEK2 and hepatocellular carcinoma (HCC) patient outcomes after liver transplants. (oncotarget.com)
  • Studies are also showing differential expression levels of miRNAs and ANGPTLs in obese versus non-obese individuals. (dasmaninstitute.org)
  • The aim of our study was to analyze the differential expression of miRNAs in the brains of BSE-infected cynomolgus macaques as a model for Creutzfeldt-Jakob disease (CJD). (biomedcentral.com)
  • These findings suggest miR-486-5p negatively regulates NEK2, which is a critical prognostic indicator of HCC patient survival after liver transplantation. (oncotarget.com)
  • We expect that most microRNAs regulated by MTp53 in H1299-MTp53 cells will also be altered after knockdown of MTp53 in the above-mentioned cell lines. (thegomap.org)
  • By employing microRNA expression profiling and functional knockdown studies on human embryonic stem cell-derived cardiomyocytes, the authors identified miR-363 as an upstream negative regulator of left ventricular specification transcription factor HAND1 . (biomedcentral.com)
  • For the identification of deregulated miRNAs we applied miRNA microarrays which have been widely used to analyze miRNA expression patterns. (biomedcentral.com)
  • These data show, for the first time, that miRNA-related genetic variations may underlie the etiology of breast cancer in both populations of African and European ancestries. (cdc.gov)
  • Noncoding RNAs, including circRNAs, microRNAs, and lncRNAs, have become a significant focus of research attention due to their crucial role in regulating the progression of multiple malignancies [ 5 ], including bladder cancer, gastric cancer, nasopharyngeal carcinoma, and pancreatic cancer. (hindawi.com)
  • However, compared to the other forms of noncoding RNA, we know very little about the precise role of circRNA in cancer. (hindawi.com)
  • Specifically, the objectives of this CIRM grant are to elucidate the role miRNAs play in the development of hESC into cells of endocrine lineage and to provide crucial details on the molecular architecture of endocrine precursor populations, lineage specification, and β-cell maturation. (ca.gov)
  • Stemming from these initial reports, ongoing investigations are evaluating the potential usefulness of miRNA-based therapy in cancer, and point to a potential role of sensitize cancer cells following chemo- and radiotherapy. (sarcomahelp.org)
  • Further exploring the role of miRNAs in cardiac cells during development and disease may therefore hold great promise for cardiac therapy applications. (biomedcentral.com)
  • In conclusion, the reduction in IAV copy number in DEFB1 overexpressing cells suggests that beta-defensin-1 plays a key role in regulating IAV survival through STAT3 and is a potential target for antiviral drug development. (cdc.gov)