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  • stem cell
  • Crist CG, Montarras D, Pallafacchina G, Rocancourt D, Cumano A, Conway SJ, Buckingham M (2009) Muscle stem cell behavior is modified by microRNA-27 regulation of Pax3 expression. (springer.com)
  • Engrailed acts with Nejire to control decapentaplegic expression in the Drosophila ovarian stem cell niche. (amedeo.com)
  • Emerging evidence suggests that miRNAs regulate critical pathways involved in stem cell function. (springer.com)
  • Comprehensively, the current intersection of aging and stem cell science indicates that if the age-imposed decline in the regenerative capacity of stem cells was understood, the debilitating lack of organ maintenance in the old could be ameliorated and perhaps, even reversed. (pubmedcentralcanada.ca)
  • Organ stem cells self-renew via asymmetric cell divisions, where one daughter cell differentiates while the other remains a stem cell. (pubmedcentralcanada.ca)
  • The regenerative capacity of organ stem cells is very efficient in embryonic and young organisms, but deteriorates with advancing age, and the cell-extrinsic changes, i.e. the aging of the stem cell niche, play a large role in that age-specific decline. (pubmedcentralcanada.ca)
  • These include resident endothelial cells that restore capillary bed function during sprouting angiogenesis ( 5 ), as well as other stem cell populations from the bone marrow compartment ( 57 ). (physiology.org)
  • A subpopulation of Pax7 + satellite cells that does not express Myf5 has been proposed to be responsible for repopulating the stem cell niche in the regenerated muscle and once again become quiescent ( 26 ). (physiology.org)
  • If the dental pulp is exposed, as long as the infection and inflammation can be controlled, conservative therapies can promote the formation of new tertiary dentine in a stem cell-led reparative process. (frontiersin.org)
  • Epigenetic modifications of DNA-associated proteins and the influences of non-coding RNAs have been demonstrated to control the self-renewal of stem cell populations as well as regulate mineralised tissue development and repair. (frontiersin.org)
  • They are involved in many biological processes such as developmental timing, differentiation, cell death, stem cell proliferation and differentiation, immune response, aging and cancer. (springer.com)
  • Accumulating studies in recent years suggest that miRNAs play crucial roles in stem cell division and differentiation. (springer.com)
  • In the present chapter, we present a brief overview of these studies and discuss their contributions toward our understanding of the importance of miRNAs in normal and aged stem cell function in various model systems. (springer.com)
  • Plasmids expressing Lef1 and ΔNLef1, a construct in which the β-catenin-binding domain of Lef1 was deleted, were used to evaluate the effects of Lef1 on stem cell differentiation. (medsci.org)
  • Lef1 overexpression promoted bulge stem cell differentiation toward a hair fate, which was accompanied by the subsequent migration of β-catenin into the nucleus, whereas no changes were observed in the control group. (medsci.org)
  • Taken together, our results demonstrate that Lef1 plays an important role in bulge stem cell differentiation, promoting β-catenin translocation into the nucleus, activating downstream signaling molecules, eventually causing hair follicle bulge stem cells to adopt the hair fate. (medsci.org)
  • A new look at the origin, function, and 'stem-cell' status of muscle satellite cells. (semanticscholar.org)
  • gene expression
  • microRNAs (miRNAs) are a group of highly conversed noncoding RNAs that regulate many biological processes by governing target gene expression [ 10 ]. (ijbs.com)
  • The changes in MRFs gene expression in muscle fibers of tambaqui from black water system provide relevant information about the environmental influence as that of water systems on gene expression of muscle growth related genes in the C. macropomum . (hindawi.com)
  • However, sarcomeric and metabolic gene expression was unaffected or upregulated in ERRα−/− cells. (physiology.org)
  • Increases in muscle protein synthesis occurring in response to resistance training can be attributed to pre-translational (increase in mRNA abundance) mechanisms [ 7 ], as muscle-specific gene expression is up-regulated in order to provide an ample supply of mRNA template to meet translational (increases in protein synthesis/unit of mRNA) demands. (biomedcentral.com)
  • This study aimed to investigate the effect of Jieduquyuziyin prescription (JP)-treated rat serum on the gene expression of MeCP2 in Jurkat T cells and its role in the pathogenesis of SLE. (sivb.org)
  • Molecular
  • The present study in pre-senile female rats, in conjunction with our previous study in young male rats, suggests that muscle remodeling and restructuring after injury may constitute the initial cellular and molecular mechanisms that underlie the benefits associated with acupuncture throughout the life-span. (scirp.org)
  • Skeletal muscle postnatal growth and repair depend on satellite cells and are regulated by molecular signals within the satellite cell niche. (rupress.org)
  • However, the molecular mechanisms involved in signaling integration within the satellite niche are still poorly understood. (rupress.org)
  • Although most immortalized adipocyte lines cannot recapitulate the process of "browning" of white fat in culture, primary adipocytes isolated from stromal vascular fraction in subcutaneous white adipose tissue (WAT) provide a reliable cellular system to study the molecular control of beige/brite cell development. (jove.com)
  • While DCIS is a non-obligate precursor to invasive breast cancers, the molecular mechanisms and cell populations that permit progression to invasive cancer are not fully known. (jove.com)
  • Research in our laboratory is focused towards understanding the molecular and signaling mechanisms that regulate the acquisition and maintenance of skeletal muscle mass. (louisville.edu)
  • Using animal models, we also study molecular mechanisms regulating skeletal muscle wasting during cancer cachexia. (louisville.edu)
  • preadipocytes
  • Positively regulates early adipogenesis of preadipocytes by promoting ARE-mediated mRNA decay of immediate early genes (IEGs). (uniprot.org)
  • Here we describe a protocol for effective isolation of primary preadipocytes and for inducing differentiation to beige/brite cells in culture. (jove.com)
  • endothelial cells
  • Fibronectin on extracellular vesicles from microvascular endothelial cells is involved in the vesicle uptake into oligodendrocyte precursor cells. (amedeo.com)
  • Promotes ARE-mediated mRNA decay of hypoxia-inducible factor HIF1A mRNA during the response of endothelial cells to hypoxia. (uniprot.org)
  • reside
  • Satellite cells (SCs), the adult skeletal muscle progenitors, reside in a niche that was anatomically defined by electron microscopy ( Mauro, 1961 ). (rupress.org)
  • Moreover, these solitary cells has been shown to disseminate from the primary tumor at an early stage of disease progression 8-10 and reside growth-arrested in the patients' bone marrow, blood and lymph nodes 1,4,11 . (jove.com)
  • reactive oxygen
  • In the transplanted regions, reactive oxygen species (ROS) can cause cell death, and this process depends on caspase activation and autophagosome formation. (biomedcentral.com)
  • After the incubation, each treatment group was evaluated for proliferation, cell cycle progression, and production of reactive oxygen species (ROS) using WST-1, flow cytometry, and CM-H2DCFDA, respectively. (sivb.org)
  • proliferate in response
  • However, these cells can proliferate in response to a variety of stimuli, and they are responsible for maintenance of the nuclear- to-cytoplasmic relationship in muscle. (physiology.org)
  • These cells, called bulge stem cells (BSCs) or hair follicle stem cells (HFSCs), are normally slow-cycling but can be stimulated to proliferate in response to injury or to certain growth stimuli. (medsci.org)
  • protein
  • Over-expression of miR-128 reduced Sp1 protein levels and inhibited muscle satellite cell proliferation and differentiation. (springer.com)
  • When a cell enters the G1 phase, a protein called cyclin D increases in response to mitogenic stimuli. (springer.com)
  • The lower skeletal muscle mass in the OZR appears to be partly accounted for by an altered balance between protein synthesis and degradation in this model of metabolic syndrome ( 4 , 16 ). (physiology.org)
  • In the article, the authors reveal that when the protein TAK1 is reduced, satellite stem cells do not vigorously self-renew and many eventually die. (eurekalert.org)
  • This publication complements research published just last week in the Journal of Clinical Investigation by Hindi and Ashok Kumar, Ph.D., a professor and distinguished university scholar in UofL's Department of Anatomical Sciences and Neurobiology, that describes the role of another protein, TRAF6 (TNF receptor-associated factor 6), in ensuring the vitality of the satellite stem cells. (eurekalert.org)
  • Lymphoid enhancer binding factor-1 (Lef1) is an essential regulatory protein in the Wnt signal pathway, which controls cell growth and differentiation. (medsci.org)
  • When stem cells were induced to differentiate toward a hair fate in a co-culture system, Lef1 was notably up-regulated and accumulated in the nucleus, appearing to activate the target protein c-myc and jagged1. (medsci.org)
  • Rather, NO-Shotgun ® effectively increases muscle strength and mass, myofibrillar protein content, and increases the content of markers indicative of satellite cell activation. (biomedcentral.com)
  • This process is critical since skeletal myocytes are multi-nucleated and each myonucleus controls both mRNA and protein synthesis over a finite sarcoplasmic volume (aka. (biomedcentral.com)
  • The authors previously reported that high pathophysiological concentrations of leptin, the adipocyte-secreted peptide, upregulates the expression of a potent proatherogenic matricellular protein, thrombospondin-1 (TSP-1), in vascular smooth muscle cells. (musclecellnews.com)
  • mitochondrial
  • For example, mTOR inactivation in mouse muscle results in severe myopathy, reduced mitochondrial biogenesis, impaired oxidative capacity, and increased glycogen content, leading to premature death [ 9 ]. (ijbs.com)
  • The estrogen-related receptors (ERRs) regulate energy substrate uptake, mitochondrial respiration, and biogenesis and may target structural gene programs in striated muscle. (physiology.org)
  • In cell-based analyses ERRα is required for induction of electron transport/oxidative phosphorylation genes and mitochondrial biogenesis in conjunction with its coactivator, peroxisome proliferator-activated receptor-γ (PPARγ) coactivator (PGC)-1α ( 37 , 53 , 68 ). (physiology.org)
  • Recent genomic studies employing transcript expression profiling and chromatin immunoprecipitation (ChIP) in heart showed that ERRα and ERRγ regulate genes involved at all steps of oxidative energy metabolism from substrate uptake through mitochondrial ATP synthesis and export ( 12 ). (physiology.org)
  • ERRα displays tissue-dependent transcriptional effects, activating mitochondrial enzyme genes in highly oxidative tissues that express PGC-1α and PGC-1β, while in white adipose and in glycolytic skeletal muscle ERRα is complexed with corepressors, such as RIP140, to repress oxidative metabolism ( 7 , 21 , 23 , 43 , 54 , 55 ). (physiology.org)
  • proximal
  • The ERRα-PGC-1α complex activates the Dusp1 gene, which encodes MKP-1, and ERRα occupies the proximal 5′ regulatory region during early differentiation in C2C12 myocytes. (physiology.org)
  • Multiminicore disease (MmD) is an autosomal recessive congenital myopathy characterized by predominantly axial and proximal muscle weakness, frequently in conjunction with progressive scoliosis and respiratory difficulties. (springer.com)