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  • aster
  • Although variable numbers of microtubules emanated from the center, more microtubules tended to polymerize onto the GFP dots in larger sizes (Fig. 4, B-D). In Fig. 4 E, the process of aster formation was monitored by time-lapse microscopy. (nih.gov)
  • BRCA1
  • Component of the BRCA1 - RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1 -mediated ubiquitination of RBBP8 . (rcsb.org)
  • the interaction ubiquitinates RBBP8 , regulates CHEK1 activation, and involves RBBP8 in BRCA1 -dependent G2/M checkpoint control on DNA damage. (rcsb.org)
  • BRCA1 nuclear transport and ubiquitin E3 ligase enzymatic activity are tightly regulated by the BRCA1 dimeric binding partner BARD1 and further modulated by cancer mutations and diverse signaling pathways. (hindawi.com)
  • AKAP350
  • The present study aimed to characterize the participation of centrosomal AKAP350 in the acquisition of migratory polarity, and the involvement of CIP4 in the pathway. (biologists.org)
  • That was enhanced in migratory cells, but inhibited in cells with decreased centrosomal AKAP350. (biologists.org)
  • sufficient
  • The results indicate that integrin-mediated adhesion is not sufficient to promote rapid microtubule regrowth in either cell type. (biologists.org)
  • The data show that Src signaling is both required and sufficient to promote rapid microtubule regrowth in cells adhered to fibronectin. (biologists.org)
  • To determine whether integrin-mediated adhesion is sufficient to promote microtubule assembly, we examined this process in primary human foreskin fibroblasts (HFFs) adhered to fibronectin in either serum-free DMEM or the serum-free, growth-promoting medium, CCM1. (biologists.org)
  • foci
  • As time progressed, more microtubules appeared to emanate from the center, indicating that microtubules were formed by direct polymerization onto the CNN-containing foci, rather than that preformed microtubules were gathered around the center. (nih.gov)
  • interaction
  • In the present study, we reinvestigated the interaction between ZW10 and dynamitin, and showed that the N-terminal region of ZW10 is the major binding site for dynamitin and, like full-length ZW10, could potentially move along microtubules to the centrosomal area in a dynein-dynactin-dependent manner. (genes2cognition.org)
  • cells
  • We assayed microtubule regrowth following nocodazole washout in human fibroblasts and CHO-K1 cells adhered to fibronectin in either normal serum-free medium or the serum-free, growth-promoting medium, CCM1, which contains IGF1 and androgen, as well as other nutrients. (biologists.org)
  • This dynamic nature of microtubules allows cells to rapidly respond to changes in their extracellular environment. (biologists.org)
  • The cells were briefly extracted with a detergent containing microtubule-stabilizing buffer before fixation. (nih.gov)
  • dependent
  • This is consistent with previous observations that the centrosomal association of CNN is not dependent on the presence of γ-tubulin/γ-TuRC (Barbosa et al. (nih.gov)