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  • transcriptional
  • Using a mouse mammary epithelial cell system, we have shown previously that conversion of epithelial cells to a fibroblastoid phenotype (epithelial-mesenchymal transition) involves downregulation of E-cadherin and upregulation of β-catenin transcriptional activity. (rupress.org)
  • Furthermore, coexpression of β-catenin or lymphoid enhancer binding factor-1 or T cell factor 3 with E-cadherin rescued β-catenin transcriptional activity and counteracted E-cadherin-mediated cell cycle arrest. (rupress.org)
  • binds
  • Binds kininogen, the proteinaceous kinin precursor, and contributes to trigger the kinin-forming cascade on the cell surface. (rcsb.org)
  • Glis1 also up-regulates expression of the protein LIN28 which binds the let-7 microRNA precursor, preventing production of active let-7. (wikipedia.org)
  • Dock180, the archetypal member of this family, is seen as an atypical GEF in that efficient GEF activity requires the presence of the DOCK-binding protein ELMO (engulfment and cell motility) which binds RhoG at its N-terminus. (wikipedia.org)
  • proteins
  • Depletion of αE-catenin also caused deep cells to have protracted plasma membrane blebbing, and a defect in plasma membrane recruitment of ERM proteins that are involved in controlling membrane-to-cortex attachment and membrane blebbing. (biologists.org)
  • Being a nodular signaling molecule, PAK1 operates to converging station of a large number of signals triggered by proteins on the cell surface as well as upstream activators, and translates into specific phenotypes. (wikipedia.org)
  • TP53 gene encodes proteins that bind to DNA and regulate gene expression to prevent mutations of the genome. (wikipedia.org)
  • BRCA1 and BRCA2 are unrelated proteins, but both are normally expressed in the cells of breast and other tissue, where they help repair damaged DNA, or destroy cells if DNA cannot be repaired. (wikipedia.org)
  • Ubiquitin is a small regulatory protein found in all tissues that direct proteins to compartments within the cell. (wikipedia.org)
  • Interestingly, the KMT2C and KMT2D proteins, rather than the KMT2C and KMT2D-mediated H3K4me1, control p300 recruitment to enhancers, enhancer activation, and transcription from promoters in embryonic stem cells. (wikipedia.org)
  • growth
  • GPI -anchored hyphal cell wall protein required for hyphal growth and virulence. (rcsb.org)
  • However, the regulatory mechanism of USP12 in human cervical carcinoma HeLa cell growth is unknown. (sigmaaldrich.com)
  • Stable expression of E-cadherin in fibroblastoid cells decreased β-catenin activity and reduced cell growth. (rupress.org)
  • PAK1 knockdown in prostate cancer cells is associated with reduced motility, reduced MMP9 secretion and increased TGFβ expression, which in these cases, is growth inhibitory. (wikipedia.org)
  • In this view, the opposing effects of ω-6 PUFA-derived and ω-3 PUFA-derived eicosanoids on key target cells underlie the detrimental and beneficial effects of ω-6 and ω-3 PUFA-rich diets on inflammation and allergy reactions, atherosclerosis, hypertension, cancer growth, and a host of other processes. (wikipedia.org)
  • In many different tumour cell lines, such as gastric cancer, medulloblastoma and endometrial cancer, lung andeocarcinoma and colorectal carcinoma, there are lower levels of miR-129 indicating that it may play a role in the suppression of cell growth. (wikipedia.org)
  • knockdown
  • In this study, we showed that knockdown of USP12 effectively induced cell cycle arrest in HeLa cells and decreased BMI-1, c-Myc and cyclin D2 transcription levels. (sigmaaldrich.com)
  • In this sense, knockdown of MEKK3 caused ovarian cancer cells to be more sensitive to drugs. (wikipedia.org)
  • mutations
  • By combining RNA interference and genetic mutations, we found that the role of CGGBP1 in cell cycle involves multiple mechanisms, as single deficiencies of CDKN1A, GAS1 as well as TP53, INK4A or ARF failed to rescue the G0/G1 arrest caused by CGGBP1 depletion. (diva-portal.org)
  • Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma. (wikipedia.org)
  • Apoptosis
  • The effect of USP12 on HeLa cell apoptosis was not observed in our study. (sigmaaldrich.com)
  • We have reported that tyrosine residues in FAK are dephosphorylated and FAK is degraded during mannitol-induced apoptosis in human neuroblastoma cells. (ebscohost.com)
  • effector
  • These results provide new insights into the mechanism of YAP mechanosensing activity and qualify this Hippo effector as the key determinant of cell mechanics in response to ECM cues. (muni.cz)
  • Here, we identified a truncated NLR protein that activates cell death in response to a specific pathogen effector. (pnas.org)
  • Arabidopsis
  • This coordination is essential in patterning the highly elongated giant cells, which are interspersed between small cells, in the outer epidermis of the Arabidopsis thaliana sepal. (biologists.org)
  • molecular
  • He observed that integrins contain a previously unappreciated molecular code which governs cell motility. (eurekalert.org)
  • Provided are embodiments of a molecular method based on guanidinium-rich molecular transporters (GR-MoTrs) for bringing molecular cargos into algal cells. (osti.gov)
  • contacting an algal cell with a guanidinium-rich delivery vehicle comprising a guanidinium-rich molecular transporter (GR-MoTr) linked to a cargo compound desired to be delivered to the algal cell, whereby the guanidinium-rich molecular transporter can traverse the algal cell wall, thereby delivering the cargo compound to the algal cell. (osti.gov)
  • roles
  • In performing these roles, eicosanoids most often act as autocrine signaling agents to impact their cells of origin or as paracrine signaling agents to impact cells in the proximity of their cells of origin. (wikipedia.org)
  • morphology
  • Dynamic Change in Morphology and Traction Forces at Focal Adhesions in Cultured Vascular Smooth Muscle Cells During Contraction. (ebscohost.com)
  • In order to study how cells change their traction forces at focal adhesions (FAs) following cell contraction, we observed the dynamic changes in traction forces at FAs, the morphology of FAs, and actin stress fibers (SFs) anchoring FAs in cultured porcine aortic smooth muscle cells (SMCs) during. (ebscohost.com)
  • PAK1 regulates cell motility and morphology. (wikipedia.org)
  • epithelial cell
  • Aim: The hypothesis that epithelial cell-specific deletion of ILK would impact on murine tumourigenesis was tested using a colitis-associated cancer model. (ebscohost.com)
  • Methods: To create intestinal epithelial cell ILK knockout animals, Fabp/Cre mice )Cre recombinase expressed under the control of a modified Fabp promoter) were used, and they were mated with mice carrying a IoxP-flanked (floxed) ILK gene )lLKflox/flox). (ebscohost.com)
  • gene transcription
  • Glis1 uses a Zinc finger domain comprising five tandem Cys2His2 zinc finger motifs (meaning the zinc atom is coordinated by two cysteine and two histidine residues) to interact with target DNA sequences to regulate gene transcription. (wikipedia.org)
  • biological
  • Sialic acid occupies the terminal end on oligosaccharide chains in these glycoproteins and influences the biological behavior of cells [ 16 ]. (medsci.org)
  • Together these results suggest that miR-155-5p is expressed in a number of tissues and cell types and, therefore, may play a critical role in a wide variety of biological processes, including hematopoiesis Although very few studies have investigated the expression levels of miR-155-3p, Landgraf et al. (wikipedia.org)
  • subsequently
  • It was observed that when cancer cells were subjected to transfection with the precursor of miR-129, GALNT1 was subsequently down regulated by the direct targeting of miR-129. (wikipedia.org)
  • cancer
  • Results: In this study we explored the role of CGGBP1 in the cell cycle in various cancer cell lines. (diva-portal.org)
  • Metastatic cancer cells usually express high density of sialic acid-rich glycoproteins on cell surfaces and help cancer cells enter the circulatory system [ 1 ]. (medsci.org)
  • No risk of cancer: Although c-myc enhances the efficiency of reprogramming, its major disadvantage is that it is a proto-oncogene meaning the iPS cells produced using c-myc are much more likely to become cancerous. (wikipedia.org)
  • This is due to the effects of the down regulation of miR-129 in cancer cells resulting in their proliferation. (wikipedia.org)
  • SOX4, SRY-related HMG box 4 gene - an oncogene that is known to be deregulated in a number of cancers, is another target of miR-129 in which the aberrant regulation of miR-129 contributes to the proliferation of cancer cell lines. (wikipedia.org)
  • In experiments where miR-129 levels were returned to normal in cancer cells, SOX4 expression was down regulated. (wikipedia.org)
  • MEKK3 is highly expressed in 4 ovarian cancer cell lines (OVCA429, Hey, DOV13, and SKOv3). (wikipedia.org)
  • This expression level is significantly higher in those cancer cells when compared to normal cells. (wikipedia.org)
  • It was observed that in a breast cancer cell line BRCA1-deficient (HCC1937), paclitaxel was unable to activate MEKK3. (wikipedia.org)
  • fibroblasts
  • Recently we had found CGGBP1 to be a cell cycle regulatory midbody protein required for normal cytokinetic abscission in normal human fibroblasts, which have all the cell cycle regulatory mechanisms intact. (diva-portal.org)
  • activity
  • KMT2D contains an enzymatically active C-terminal SET domain that is responsible for its methyltransferase activity and maintaining protein stability in cells. (wikipedia.org)
  • In addition, mutation of Y5477 in mouse KMT2D, which corresponds to Y5426 in human KMT2D, resulted in the inactivation of KMT2D's enzymatic activity in embryonic stem cells. (wikipedia.org)
  • expression
  • Over-expression of miR-33 induced a significant G1 cell cycle arrest and most importantly, inhibition of miR-33 expression using 2'F/MOE-modified phosphorothioate backbone antisense oligonucleotides improved liver regeneration after partial hepatectomy (PH) in mice, suggesting an important role for miR-33 in regulating hepatocyte proliferation during liver regeneration. (hu-berlin.de)
  • Furthermore, we found that increasing Rac V12 expression results in a biphasic proliferative curve, with proliferation initially increasing as Rac V12 expression increases and then returning to levels less than that of control cells at higher expression. (pubmedcentralcanada.ca)
  • Finally, we show in vivo that Rac-induced TSP2 expression is observed in the aorta of transgenic mice selectively expressing Rac V12 in smooth muscle cells. (pubmedcentralcanada.ca)
  • CHO cell line was derived from the Chinese hamster ovary and can provide a high expression of recombinant glycoproteins which are equipped with a glycosylation mechanism very similar to that found in humans [ 15 ]. (medsci.org)
  • BTG1 expression is maximal in the G0/G1 phases of the cell cycle and downregulated when cells progressed through G1. (wikipedia.org)
  • It was demonstrated that miR-155 over-expression supports the development and aggresivness of B cell malignancies. (wikipedia.org)
  • Sequence analysis of small RNA clone libraries comparing miRNA expression to all other organ systems examined established that miR-155-5p was one of five miRNAs (i.e. miR-142, miR-144, miR-150, miR-155, and miR-223) that was specific for hematopoietic cells including B-cells, T-cells, monocytes and granulocytes. (wikipedia.org)
  • established that expression levels of this miRNA was very low in hematopoietic cells. (wikipedia.org)
  • This is because of its integral role it plays in the proliferation of cells and its many recognisable, statistically significant and independent interactions with its targets as observed by the expression profiles in the study carried out by Ogawa et al. (wikipedia.org)
  • inhibition
  • Inhibition in live cells supports a critical role for PAK in PDGF-stimulated ERK activation. (wikipedia.org)
  • PFK is able to regulate glycolysis through allosteric inhibition, and in this way, the cell can increase or decrease the rate of glycolysis in response to the cell's energy requirements. (wikipedia.org)
  • mechanism
  • These patterning processes take place while cells are growing and proliferating, so coordination of cell type with cell cycle control is essential, but we are only beginning to understand its mechanism. (biologists.org)
  • Our new observation demonstrate for the first time the mechanism which enables cells to specifically turnover their adhesions to migrate in a specific tissue. (eurekalert.org)
  • immune
  • Involved in innate immune cell evasion through confering resistance to neutrophil killing. (rcsb.org)
  • significantly
  • Another 2011 study found that the p53 homozygous (Pro/Pro) genotype was associated with a significantly increased risk for renal cell carcinoma. (wikipedia.org)
  • knockout
  • Targeted knockout of Kmt2d in precursors cells of brown adipocytes and myocytes results in decreases in brown adipose tissue and muscle mass in mice, indicating that KMT2D is required for adipose and muscle tissue development. (wikipedia.org)