• MicroRNAs (miRNAs) are small noncoding endogenous RNAs containing 19 to 25 nucleotides that promote posttranscriptional control in regulating the expression of target gene by binding to the 3′-UTR sequences of its mRNA [ 5 ]. (hindawi.com)
  • Recently, increasing evidences elucidated that miRNAs was involved in the cancer pathogenesis, including cell proliferation, metastasis, and apoptosis [ 6 ]. (hindawi.com)
  • MicroRNAs (miRNAs) have become a hot topic in cancer research, but the underlying mechanism of its involvement in cancer remains unclear. (researchsquare.com)
  • Recent studies, including our previous findings, have demonstrated that miRNAs play an important role in regulating biological functions [6-8]. (researchsquare.com)
  • Because of their unique expression profile and special functions, miRNAs have been used as molecular markers to define the direction and processes of cell differentiation by regulating one or several target genes. (researchsquare.com)
  • MiRNAs can also control the self-renewal or proliferation of stem cells [9]. (researchsquare.com)
  • Previous studies have confirmed that miRNAs play an important role in gene regulation in CD44 + CD24 -/low breast cancer stem cells (BCSCs). (researchsquare.com)
  • MicroRNAs (miRNAs, miRs) are a class of small, non-coding, endogenous and functional RNAs. (biomedcentral.com)
  • Mature miRNAs are generated from sequential processing of primary miRNA transcripts by Drosha and Dicer, then serve as posttranscriptional regulators of gene expression in certain biological events including carcinogenesis [ 10 ]. (biomedcentral.com)
  • Another level of regulation may lie on microRNAs (miRNAs), which are endogenously expressed small non-coding RNAs of 18-24 nucleotides in length that regulate gene expression on the posttranscriptional level [ 14 ]. (biomedcentral.com)
  • microRNAs also play a crucial role in endothelial inflammation, senescence and susceptibility to atherosclerosis: endothelial inflammation is critically regulated by miRNAs such as miR-126 and miR-10a, and endothelial aging is additionally controlled by miR-217 and miR-34a [ 15 ]. (biomedcentral.com)
  • In recent years, emerging studies have highlighted the critical role of these pathways and their regulation by microRNAs (miRNAs) in cancer invasion and metastasis. (intechopen.com)
  • MiRNAs, short (18-24 nucleotides) non-coding RNAs, are derived from long transcripts pri-miRNAs and pre-miRNAs [ 26 - 30 ].By targeting 3' untranslated regions (3'UTRs) of cognate mRNAs, miRNAs post-transcriptionally regulate gene expression and induce translational repression [ 29 , 30 ]. (intechopen.com)
  • To date, 1527 human miRNAs have been identified (Sanger miRBase 18 http://www.miRbase.org/index.shtml), forming less than 1% of all human genes, potentially regulating more than 10% of all protein coding genes [ 1 ]. (intechopen.com)
  • microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
  • miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (cancerindex.org)
  • Feng, Y Abstract: BACKGROUND: Accumulating studies have indicated the involvement of microRNAs (miRNAs) in the progression and treatment of human hepatocellular carcinoma (HCC). (hku.hk)
  • miRNAs mediate drug resistance of HCC and regulating miRNAs may give rise to novel strategy of treatment of HCC. (hku.hk)
  • We aimed to evaluate the selected miRNAs expression based on bioinformatic analysis and their changes before and after an ultramar. (researchgate.net)
  • A large number of studies have demonstrated that the synergistic collaboration of a number of microRNAs (miRNAs), their growth factors and their downstream agents is required for the initiation and completion of pathogenesis in the liver. (wjgnet.com)
  • Emerging evidence indicates that Long non-coding RNAs (LncRNAs) and microRNAs (miRNAs) play crucial roles in tumor progression, including hepatocellular carcinoma (HCC). (biomedcentral.com)
  • A series of studies indicate that lncRNAs serve as competing endogenous RNAs (ceRNA) by sponging miRNAs, and modulate the targets of miRNAs [ 15 , 16 ]. (biomedcentral.com)
  • MicroRNAs (miRNAs) are small endogenous RNAs that are associated with tumourigenesis, cell proliferation and apoptosis ( 4 , 5 ). (spandidos-publications.com)
  • The aberrant expression of miRNAs is associated with the pathogenesis of sepsis. (degruyter.com)
  • MicroRNAs (miRNAs) are a kind of small noncoding RNAs that regulate the expression of protein through targeting the 3′UTR of messenger RNA (mRNA), resulting in mRNA degradation or suppression of translation [ 5 ]. (degruyter.com)
  • In our research, microRNAs (miRNAs) binding to the genome sequences of severe acute respiratory syndrome coronavirus (SARS-CoV), Middle East respiratory-related coronavirus (MERS-CoV), and SARS-CoV-2 were identified by bioinformatic tools. (molcells.org)
  • The expression patterns of those miRNAs were examined in hamster lung samples infected by SARS-CoV-2. (molcells.org)
  • Five miRNAs (hsa-miR-15b-5p, hsa-miR-195-5p, hsa-miR-221-3p, hsa-miR-140-3p, and hsa-miR-422a) showed differential expression patterns in lung tissues before and after infection. (molcells.org)
  • MicroRNAs (miRNAs) are a class of small non-coding RNA, 20-22 nucleotides in length. (molcells.org)
  • The seed region in miRNAs, approximately six nucleotides long, recognizes and binds to the complementary binding site of the target mRNA 3'untranslated region (3'UTR) to regulate its expression. (molcells.org)
  • MicroRNAs (miRNAs) are master regulators involved in multiple physiological and pathological processes. (genengnews.com)
  • It's been suggested that miRNAs regulate up to 50% of all mRNAs in the human genome. (genengnews.com)
  • Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
  • The involvement of miRNAs in cancer research emerged from studies demonstrating their aberrant expression in neoplastic tissues. (genengnews.com)
  • MicroRNAs (miRNAs) are well-recognized for their abilities to regulate gene expression post-transcriptionally in plants and animals. (frontiersin.org)
  • The expressions of candidate miRNAs and mRNAs were determined by real-time quantitative PCR(RT-qPCR) in MC3T3-E1 cells. (frontiersin.org)
  • Murine hepatic miRNAs expression and regulation of gene expression in diet-induced obese mice. (genosensor.co)
  • In the case of RNA molecules that bind target RNA segments, such as microRNAs (miRNAs), the altered base pairing can change binding specificities. (biomedcentral.com)
  • DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)
  • Simple Summary: This study identified a set of 73 microRNAs (miRNAs) that can accurately detect lung cancer tumors from normal lung tissues. (cdc.gov)
  • Based on the consistent expression patterns associated with patient survival outcomes and in tumors vs. normal lung tissues, 10 miRNAs were considered to be putatively tumor suppressive and 4 miRNAs were deemed as oncogenic in lung cancer. (cdc.gov)
  • This study identified a set of 73 microRNAs (miRNAs) that classified lung cancer tumors from normal lung tissues with an overall accuracy of 96.3% in the training patient cohort (n = 109) and 91.7% in unsupervised classification and 92.3% in supervised classification in the validation set (n = 375). (cdc.gov)
  • Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
  • This protein contains two N-terminal RNA-recognition motifs (RRMs), consistent with the observation that it binds directly to pre-mRNA. (nih.gov)
  • Luciferase reporter assay was performed to verify that miR-455 directly binds to the 3′-noncoding region (UTR) of RAB18 mRNA in Huh7 cells. (hindawi.com)
  • miR-455 inhibited cell viability and invasion by directly targeting the 3′-UTR of RAB18 mRNA of hepatocellular carcinoma. (hindawi.com)
  • Scheme of putative microRNA-18a (miR-18a) binding sites in the Dicer Ⅰ mRNA 3'-untranslated region (3'-UTR). (cams.cn)
  • miR-18a post-transcriptionally downregulates Dicer Ⅰ expression by directly targeting the Dicer Ⅰ mRNA 3′-UTR. (cams.cn)
  • The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (cancerindex.org)
  • At last, the mRNA and protein expression of NFAT2, Egr2, FasL, COX-2 and c-myc in carcinoma and adjacent tissues was investigated. (researchsquare.com)
  • These naturally occurring, noncoding, single-stranded RNAs (21-25 nucleotides long) base-pair with their target mRNA within the RISC (RNA-induced silencing complex). (genengnews.com)
  • According to Dr. Marcusson, an important aspect of these studies was the demonstration of miR-21 knockdown using genome-wide mRNA expression studies. (genengnews.com)
  • Recently, miRNA-messenger RNA (mRNA) regulatory relationships have been confirmed during biological processes, including osteogenic differentiation. (frontiersin.org)
  • This study aimed to find out more candidate miRNA-mRNA pairs involved in the osteogenic differentiation of MC3T3-E1 cells. (frontiersin.org)
  • There were 7 overlapped miRNA-mRNA pairs identified during osteogenic differentiation of MC3T3-E1 cells, including mmu-miR-204-5p-Arhgap11a, mmu-miR-211-5p-Arhgap11a, mmu-miR-24-3p-H2afx, mmu-miR-3470b-Chek2, mmu-miR-3470b-Dlgap5, mmu-miR-466b-3p-Chek1, and mmu-miR-466c-3p-Chek1. (frontiersin.org)
  • 18 DERs and DEDs were identified in the PTM vs. PM, 87 in the TM vs. PTM, and 87 in the TM vs. PM. Those DERs and DEDs participated in the regulation of gene expression at the levels of chromatin conformation, gene activation and silencing, splicing and degradation of mRNA, biogenesis of piRNA and miRNA, ribosome assemble, and translation of proteins. (peerj.com)
  • Pansensitive and panresistant genes to 21 NCCN-recommended drugs with concordant mRNA and protein expression were identified. (cdc.gov)
  • Mechanistically, PTTG3P upregulated the expression of miR-383 targets Cyclin D1 (CCND1) and poly ADP-ribose polymerase 2 (PARP2) by sponging miR-383, acting as a competing endogenous RNA (ceRNA). (biomedcentral.com)
  • However, it is still enigmatic about the exact processes in which RBPs-encoding genes and the relevant genes participate in transcriptome level during the biogenesis of secondary tumor in the activity of hepatocellular carcinoma (HCC) cells. (peerj.com)
  • The target genes were predicted by the online programs picTar, miRanda and TargetScan and verified by dual luciferase assay, and changes in protein expression were detected by western blot. (researchsquare.com)
  • EPCs from CB expressed abundant genes involved in cell cycle, hypoxia signalling and blood vessel development, correlating with the phenotypes that CB-EPCs proliferated more rapidly, migrated faster, and formed tubule structure more efficiently. (biomedcentral.com)
  • Stimulating the expression of angiogenic microRNAs or genes in EPCs of low activity (such as those from patients with cardiovascular diseases) might allow the development of novel therapeutic strategies. (biomedcentral.com)
  • Our analysis revealed a number of genes previously associated with hepatocellular carcinoma and uncovered their association network in the blood. (frontiersin.org)
  • WGCNA tries to find genes that have correlated expressions and aims to build sets of such genes which are named modules. (frontiersin.org)
  • This correlation indicates that linked genes are likely part of a shared regulatory mechanism in the cell. (frontiersin.org)
  • In comparison to the frequently used differential gene expression analysis the approach offers additional insights by incorporating information about the relationship between genes detected in a given sample. (frontiersin.org)
  • qPCR and Western blot were used to analyze LncRNA PTTG3P, miR-383 and other target genes' expression. (biomedcentral.com)
  • Identification of miRNA-regulated targeting genes is important for understanding their specific biological functions [ 11 ]. (degruyter.com)
  • This study aimed to evaluate the expression levels of miR-34a and 11 of its bioinformatically selected target genes and proteins to test their potential dysregulation in RCC. (hindawi.com)
  • Over the past few years, emerging numerous bioinformatic tools have been developed to identify candidate disease-causing genes [ 4 ], including microRNA (miRNA) genes. (hindawi.com)
  • An aberrant miRNA expression could contribute to cancer development and progression [ 6 , 7 ] and could affect their target genes that are involved in many biological processes, such as cell differentiation, proliferation, apoptosis, metabolism, and development [ 8 ]. (hindawi.com)
  • Gene expression datasets (GSE121248, GSE40873, GSE62232) were used to identify differentially expressed genes (DEGs) between tumor and adjacent or normal tissues, and then hub genes were screened by protein-protein interaction (PPI) network and Cytoscape software. (biomedcentral.com)
  • We previously identified differentially expressed microRNAs with liver-specific target genes in plasma from children with chronic hepatitis B. To further understand the biological role of these microRNAs in the pathogenesis of chronic hepatitis B, we have used the human liver cell line HepG2, with and without HBV replication, after transfection of hepatitis B virus expression vectors. (biomedcentral.com)
  • RT-qPCR is the preferred method for microRNA studies, and a careful normalisation strategy, verifying the optimal set of reference genes, is decisive for correctly evaluating microRNA expression levels. (biomedcentral.com)
  • The aim of this study was to provide valid reference genes for the human HCC-derived cell line HepG2. (biomedcentral.com)
  • A panel of 739 microRNAs was screened to identify the most stably expressed microRNAs, followed by a PubMed search identifying microRNAs previously used as reference genes. (biomedcentral.com)
  • We identified miR-24-3p, miR-151a-5p, and miR-425-5p as the most valid combination of reference genes for microRNA RT-qPCR studies in our hepatitis B virus replicating HepG2 cell model. (biomedcentral.com)
  • The Arhgap11a, H2afx, Chek2, Dlgap5, and Chek1 were hub genes downregulated in MC3T3-E1 cells after osteogenic differentiation, verified by RT-qPCR results. (frontiersin.org)
  • It regulates Sp7 protein expression and induces expression of major bone matrix protein genes, such as Col1a1, Spp1, Ibsp, Bglap2, and Fn1 ( 7 , 8 ). (frontiersin.org)
  • ADARs have been shown to contribute to disease pathologies by editing of glutamate receptors, editing of serotonin receptors, mutations in ADAR genes, and by other mechanisms, including recently identified regulatory roles in microRNA processing. (biomedcentral.com)
  • This study is to explore the biogenesis mechanism of a secondary hepatocellular carcinoma (HCC) based on the function of RNA binding proteins (RBPs)-encoding genes in the physiological microenvironment (PM). (peerj.com)
  • The genes encoding RBPs and the relevant genes are involved in the transformation from PM to PTM, then constructing the TM by regulating protein synthesis. (peerj.com)
  • miR-26a expression is down-regulated in human lung cancer tissues relative to normal tissues. (wikipedia.org)
  • The expression of miR-455 was lower in HCC tissues and cell lines than in nontumor tissues and normal cell line, and downregulation of miR-455 was connected with worse outcome of HCC patients. (hindawi.com)
  • RAB18 was upregulated in HCC tissues and cell lines, and the expression of RAB18 was regulated by miR-455. (hindawi.com)
  • All the fresh tissues were snap-frozen in liquid nitrogen and stored at −80°C until RNA extraction. (hindawi.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • Analysis of multiple microarrays in the Oncomine database revealed that NEK2 expression was higher in HCC tissues than adjacent normal liver tissues. (oncotarget.com)
  • In this study, we found that PTTG3P was up-regulated in HCC tissues and cells. (biomedcentral.com)
  • Transforming growth factor-β (TGF-β) signaling pathway is a multifunctional cytokine and plays important roles in regulating cell proliferation, cell cycle, differentiation, migration, and apoptosis in a broad spectrum of tissues [ 12 ]. (degruyter.com)
  • We found RCC tissues displayed significantly higher miR-34a expression level than their corresponding noncancerous tissues, particularly in chromophobic subtype. (hindawi.com)
  • MIR-34A has its own transcript and is expressed at higher levels than MIR-34B/C in most tissues, and this expression could be dysregulated in multiple diseases, especially in cancers [ 11 ]. (hindawi.com)
  • In this study, we found that LIM-only protein 3 (LMO3) is hypomethylated and overexpressed in glioma cells and tissues. (oncotarget.com)
  • Compared with adjacent tissues, the carcinoma tissues expressed less NFAT2, Egr2, FasL and more COX-2 and c-myc. (researchsquare.com)
  • The absence of NFAT2 and Egr2 in carcinoma tissues reminded us that NFAT2 may be a promising therapeutic target for hepatocellular carcinoma treatment. (researchsquare.com)
  • A-to-I editing, which is catalyzed by enzymes of the adenosine deaminase acting on RNA (ADAR) family, is most prevalent in the central nervous system (CNS) but occurs in many tissues [ 1 - 3 ]. (biomedcentral.com)
  • HCC tissues from patients had higher levels of PVRL1 messenger RNA and protein than nontumorous tissues. (stanford.edu)
  • The RNAs play important roles in the pathological and physiological tissues including cancer. (peerj.com)
  • Overexpression of miR-26a brings about negative regulation of both cell proliferation and of the cell cycle. (wikipedia.org)
  • Meanwhile, the overexpression of miR-26a in the A549 human lung cancer cell line dramatically inhibits cell proliferation, blocks G1/S phase transition, induces apoptosis, and inhibits cell metastasis and invasion in vitro. (wikipedia.org)
  • miR-455 suppressed cell proliferation in vitro and in vivo, and it inhibited the abilities of cell invasion and EMT in HCC. (hindawi.com)
  • Also, in gastric cancer, miR-455 inhibited human cell proliferation and invasion and promoted cell apoptosis [ 12 ]. (hindawi.com)
  • Then, agomir-20b-5p and antagomir-20b-5p were transfected into MCF-7 and T47D breast cancer cells to detect cell migration, wound healing and proliferation, and lentivirus vectors silencing or overexpressing miR-20b-5p were transfected into T47D-CSCs to detect proliferation and apoptosis. (researchsquare.com)
  • We have identified a single microRNA that controls glioma cell proliferation, migration, and responsiveness to glucose deprivation. (researchgate.net)
  • Further, high NEK2 expression promoted proliferation, colony formation, migration and invasion of HCC cell lines. (oncotarget.com)
  • CCK-8 assay was performed to examine cell proliferation. (biomedcentral.com)
  • Functional experiments demonstrated that knockdown of PTTG3P inhibited cell proliferation, migration and invasion, and promoted cell apoptosis, acting as an oncogene. (biomedcentral.com)
  • Then, the chemical count kit-8 cell viability assay, Annexin V-FITC apoptosis detection, EdU labeling proliferation detection, transwell and wound healing experiments were performed. (researchsquare.com)
  • Therefore, besides the regulation of immune cells, NFAT2 also exerts modulatory effect of proliferation, invasion, metastasis and malignancy in breast cancer, lung cancer, melanoma and leukemia [13-15]. (researchsquare.com)
  • In this study, we constructed NFAT2 overexpressed cells (HepG2/NFAT2) based on HepG2 and investigated the effect of NFAT2 overexpression on HepG2 cells' viability, apoptosis, proliferation, invasion and migration. (researchsquare.com)
  • MicroRNA-29b-2-5p inhibits cell proliferation by directly targeting Cbl-b in pancreatic ductal adenocarcinoma. (genosensor.co)
  • The miR-26 microRNA is a small non-coding RNA that is involved in regulating gene expression. (wikipedia.org)
  • Inhibition of miRNA-26a increases gene expression of SMAD-1 and SMAD-4, while overexpression inhibits SMAD-1. (wikipedia.org)
  • Ectopic expression of miR-26a influences cell cycle progression by targeting the bona fide oncogene EZH2 which is a polycomb protein and global regulator of gene expression. (wikipedia.org)
  • MiR-20b-5p had the highest degree in both the miRNA-gene network and miRNA-GO network to regulate BCSCs. (researchsquare.com)
  • EPCs from human umbilical cord and adult peripheral blood activate different mechanisms upon high-dose x-ray radiation treatment: CB-EPCs undergo p53 stabilization, Bax-dependent apoptosis and p21-dependent G 1 and G 2 /M cell cycle checkpoints, while PB-EPCs undergo only radiation-induced senescence [ 13 ], indicating unique gene expression patterns in EPCs of different sources. (biomedcentral.com)
  • This miRNA functions as a tumor suppressor and dysregulation or loss of the host gene from which this miRNA is processed is associated with cancer progression in numerous cell types. (cancerindex.org)
  • For example, 5-methylcytosine methylation in DNA has affected gene expression in many tumours. (biomedcentral.com)
  • In this study, we aimed at characterizing extracellular transcriptome gene networks of hepatocellular carcinoma patients compared to healthy controls. (frontiersin.org)
  • Our study thus demonstrates the feasibility of performing gene co-expression network analysis from cell-free RNA data and its utility in studying hepatocellular carcinoma. (frontiersin.org)
  • An underexplored way of analyzing the rich data obtained from sequencing liquid biopsy-derived cfRNA liquid biopsy is weighted gene co-expression network analysis (WGCNA). (frontiersin.org)
  • Immune cells were detected by flow cytometry and feces samples were collected for 16S rRNA gene amplicon sequencing. (hku.hk)
  • Pseudogene, a subclass of long non-coding RNAs (lncRNAs), are considered as genomic loci that resemble real gene, but lost some functionality because they are lack of protein-coding ability because of disabling mutation, lack of transcription, or their inability to encode RNA [ 3 ]. (biomedcentral.com)
  • This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in posttranscriptional silencing of the gene expression [ 5 ]. (hindawi.com)
  • MicroRNA-34a gene (MIR-34A) that is located on chromosome 1p36 belongs to one of evolutionary-conserved miRNA families (MIR-34 family) that consists of three members: MIR-34A, MIR-34B, and MIR-34C [ 10 ]. (hindawi.com)
  • The purpose of this study was to use the gene expression data in Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) database to develop a multigene model to predict the prognosis of patients with HCC. (biomedcentral.com)
  • In this study, three raw gene expression profiles (GSE121248, GSE40873, GSE62232) were downloaded from GEO database ( https://www.ncbi.nlm.nih.gov/geo ) ( 8 ). (biomedcentral.com)
  • The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. (cancer-genetics.org)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancer-genetics.org)
  • Reference gene stabilities were calculated first by standard deviations of ΔCt values and then by geNorm and NormFinder analyses, taking into account the amplification efficiency of each microRNA primer set. (biomedcentral.com)
  • Even small changes in microRNA expression may therefore have implications for gene regulation in various physiological and pathophysiological states [ 1 , 6 ]. (biomedcentral.com)
  • The small nuclear RNA U6 is frequently used as a reference gene (RG) in such studies [ 20 ]. (biomedcentral.com)
  • An MC3T3-E1-based microarray dataset (accessioned as GSE46400) downloaded from the Gene Expression Omnibus included MC3T3-E1 cells with or without 14-day osteoblast differentiation osteoblast induction. (frontiersin.org)
  • MicroRNA 429 Regulates Mucin Gene Expression and Secretion in Murine Model of Colitis. (genosensor.co)
  • Furthermore, bioinformatics approaches are employed to provide in silico evidences for the link between long noncoding RNAs and mitochondrial gene interaction in obesity. (informaticsstudies.org)
  • Abstract Oral squamous cell carcinoma (OSCC) is a malignant tumor triggered by the accumulation of multiple gene mutations in oral epithelial cells. (techscience.com)
  • Significant downregulation of beta-defensin1 gene (DEFB1) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
  • Hepatocellular carcinoma miR-26a has been found to induce cell cycle arrest at the G1 phase in human hepatocellular carcinoma cells, in part through direct downregulation of cyclin D2 and cyclin E2. (wikipedia.org)
  • Therapeutic miR-26a delivery using adeno-associated virus (AAV) is able to inhibit cancer cell formation while also inducing tumour-specific apoptosis and providing dramatic protection from disease progression without toxicity. (wikipedia.org)
  • It directly represses expression of the oncogene EZH2 (enhancer of zeste homolog 2), which in turn causes inhibition of cell growth and cell-cycle progression. (wikipedia.org)
  • It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. (thermofisher.com)
  • The transition from ductal carcinoma in situ to invasive ductal carcinoma is a key event in breast cancer progression that is still not well understood. (researchgate.net)
  • TRIM32 promotes oral squamous cell carcinoma progression by enhancing FBP2 ubiquitination and degradation. (nih.gov)
  • miR-26 expression is induced in response to hypoxia and upregulated during smooth muscle cell (SMC) differentiation and neurogenesis. (wikipedia.org)
  • Smooth muscle cell (SMC) differentiation miRNA-26a is found to be significantly upregulated during SMC differentiation and downregulated in abdominal aortic aneurysm (AAA) formation. (wikipedia.org)
  • microRNAs have emerged as master regulators of stem cell lineage differentiation and angiogenesis [ 14 ]. (biomedcentral.com)
  • and cell differentiation ( SOX2 and TGFB3 ) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. (hindawi.com)
  • It is involved in p53 pathways and is implicated in cell death/survival signaling, the cell cycle, and differentiation, thereby playing a regulatory role in carcinogenesis [ 12 ]. (hindawi.com)
  • Nuclear factor of activated T cells 2 (NFAT2, also known as NFATc and NFATc1) is an important member of NFAT family which plays a vital role in T cell activation and differentiation [4]. (researchsquare.com)
  • This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. (cancer-genetics.org)
  • The MC3T3-E1 cells were cultured and incubated in the osteogenic differentiation medium for 14 days. (frontiersin.org)
  • Our study characterizes miR-211-5p targeting Arhgap11a promotes the osteogenic differentiation of MC3T3-E1 cells, which provides novel targets to promote the osteogenesis process during bone repair. (frontiersin.org)
  • Osterix (OSX) is a transcription factor in the SP family, which is essential for osteoblast differentiation, and its expression is closely related to Runx2 ( 9 ). (frontiersin.org)
  • In this review, we focus on the relationship between RNA m 6 A methylation and gastrointestinal cancer, especially their role, mechanism and potential clinical application as biomarkers and therapeutic targets for gastrointestinal cancer. (biomedcentral.com)
  • Recent studies reported microRNAs as promising biomarkers for early cancer detection, accurate prognosis, and molecular targets for future treatment. (hindawi.com)
  • Therefore, research on hepatocellular transformations in initiation and development of HCC and identifying effective biomarkers for novel therapeutic option are still emergency challenges. (researchsquare.com)
  • Especially, hsa-miR-15b-5p and hsa-miR-195-5p showed a large difference in expression, indicating that they may potentially be diagnostic biomarkers for SARS-CoV-2 infection. (molcells.org)
  • MicroRNAs are regulatory molecules and suggested as non-invasive biomarkers for molecular diagnostics and prognostics. (biomedcentral.com)
  • MicroRNAs are suggested as non-invasive biomarkers for molecular diagnostics and prognostics, and some microRNAs show promising results as therapeutic targets in human trials [ 1 - 3 ]. (biomedcentral.com)
  • miR-1254 and miR-574-5p: serum-based microRNA biomarkers for early-stage non-small cell lung cancer. (genosensor.co)
  • Abstract Circulating tumor cells (CTCs) are essential biomarkers for liquid biopsies, which are important in the early screening, prognosis, and real-time monitoring of cancer. (techscience.com)
  • Circulating long non-coding RNAs NKILA, NEAT1, MALAT1, and MIAT expression and their association in type 2 diabetes mellitus. (informaticsstudies.org)
  • Thus, umbilical cord blood EPCs hold great therapeutic potential for cell therapy and vascular engineering. (biomedcentral.com)
  • miR-128-3p could inhibit apoptosis and inflammation by targeting TGFBR2 in LPS-induced HK2 cells, which might provide therapeutic strategy for the treatment of sepsis. (degruyter.com)
  • Researchers at Regulus Therapeutics described how one such miRNA (miR-21) may provide a novel therapeutic target for hepatocellular carcinoma. (genengnews.com)
  • m 6 A is catalysed by RNA methyltransferases METTL3, METTL14 and WTAP (writers), m 6 A is removed by the demethylases FTO and ALKBH5 (erasers) and interacts with m6A-binding proteins, such as YT521-B homology (YTH) domain-containing proteins. (biomedcentral.com)
  • Inhibition of miR‑132 activated PGC‑1α/Nrf2 signalling and inhibited oxidative stress and the expression of the pyroptosis‑related proteins NLRP3, caspase‑1 and IL‑1β, which were all reversed by inhibiting SIRT1 with EX527. (spandidos-publications.com)
  • The primary barriers for invading respiratory pathogens are the respiratory tract epithelial cells and antimicrobial proteins generated by these cells. (cdc.gov)
  • It further induces apoptosis, inhibition of colony formation and tumorigenesis of breast cancer cells in vivo. (wikipedia.org)
  • A decrease in MTDH and EZH2 expression has been shown to be accompanied by an increase in apoptosis, whilst re-expression of MTDH partially reverses miR-26a's pro-apoptotic effect. (wikipedia.org)
  • In addition, miR-455 inhibited cell viability, while it induced cell apoptosis in colorectal cancer [ 13 ]. (hindawi.com)
  • Annexin V-PE/PI and PI staining were used to analyze cell apoptosis and cell cycle distribution by flow cytometry, respectively. (biomedcentral.com)
  • Myocardial I/R injury may induce cell apoptosis and autophagy by activating oxidative stress and upregulating inflammatory mediators, ultimately resulting in irreversible fibrotic damage ( 3 ). (spandidos-publications.com)
  • Cell apoptosis was analyzed by flow cytometry. (degruyter.com)
  • Moreover, TGFBR2 was a direct target of miR-128-3p, and its overexpression reversed the inhibitory effects of miR-128-3p overexpression on inflammation and apoptosis in LPS-induced HK2 cells. (degruyter.com)
  • The NFAT2 overexpression suppressed the cell viability, invasion and migration capabilities, and promoted apoptosis of HepG2 cells. (researchsquare.com)
  • In addition to DNA and histone methylation, another level of epigenetic regulation, namely, RNA methylation, has become a hot topic in biosciences over the past decade. (biomedcentral.com)
  • MicroRNAs mediate regulation of chemoresistance of hepatocellular carcinoma cells with involvement of p53 tumour suppressor. (hku.hk)
  • Osteoblast mainly originates from mesenchymal stem cells (MSCs), and it is involved in bone formation, remodeling, and reconstruction via the formation of a bone matrix and regulation of bone resorption ( 6 ). (frontiersin.org)
  • Cell invasion was evaluated by transwell invasion assay, and cell migration was detected by transwell migration and wound-healing assays. (cams.cn)
  • Moreover, luciferase reporter assay was used to identify whether Dicer expression was regulated by miR-18a. (cams.cn)
  • In addition, a functional restoration assay was performed to investigate whether miR-18a promotes HCC cell migration and invasion by directly targeting Dicer 1. (cams.cn)
  • Transwell matrigel invasion assay of HepG2 cells (C) or HepG2.2.15 cells (D) expressing miR-18a inhibitor or inhibitor NC. (cams.cn)
  • Luciferase reporter assay and RNA pull-down assay were carried out to detect the interaction between miR-383 and LncRNA PTTG3P. (biomedcentral.com)
  • The binding sites of miR-128-3p and TGFBR2 were predicted by Targetscan online software and confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. (degruyter.com)
  • Moreover, miR-26 is consistently down-regulated in a wide range of malignant tumors, such as hepatocellular carcinoma, nasopharyngeal carcinoma, lung cancer, and breast cancer. (wikipedia.org)
  • Oncogenic miR-20b-5p was confirmed to promote the malignant behaviors of breast cancer cells and BCSCs. (researchsquare.com)
  • Hepatocellular carcinoma (HCC) is a malignant cancer with an observable heterogeneity and microRNAs are functionally associated with the tumorigenesis of HCC. (biomedcentral.com)
  • Hepatocellular carcinoma (HCC) is the fifth most common malignant cancer and the third leading cause of cancer death worldwide with the observable heterogeneity in its morphology, clinical behaviour, and molecular profiles [ 1 ]. (biomedcentral.com)
  • Malignant tumor, as a new "system" in the body, is gestated by the organic environment which is the time and space for a tumor system to survive and to proliferate, especially the microenvironment that is in close contact with cancer cells. (peerj.com)
  • As a result, ADAR-mediated editing can post-transcriptionally alter codons, introduce or remove splice sites, or affect the base pairing of the RNA molecule with itself or with other RNAs. (biomedcentral.com)
  • Chemical modifications to provide drug-like properties to RNA molecules are used in the synthesis of both siRNAs and antimicroRNAs (antimiRs). (genengnews.com)
  • Accumulating evidences have elucidated connection between the expression of GTPases members and several diseases, including colorectal cancer, lung cancer, and prostate cancer [ 16 - 18 ]. (hindawi.com)
  • Role of Dusp6 Phosphatase as a Tumor Suppressor in Non-Small Cell Lung Cancer. (cancer-genetics.org)
  • MicroRNA-328 is associated with (non-small) cell lung cancer (NSCLC) brain metastasis and mediates NSCLC migration. (genosensor.co)
  • Based on our identified miRNA-regulated network, we discovered three drugsBX-912, daunorubicin, and midostaurinthat can be repositioned to treat lung cancer, which was not known before. (cdc.gov)
  • Based on our identified miRNA-regulated molecular machinery, an inhibitor of PDK1/Akt BX-912, an anthracycline antibiotic daunorubicin, and a multi-targeted protein kinase inhibitor midostaurin were discovered as potential repositioning drugs for treating lung cancer. (cdc.gov)
  • miR-26a also directly suppresses expression of estrogen receptor alpha (Erα). (wikipedia.org)
  • miR-26a again suppresses tumorigenesis in nasopharyngeal cells in vivo, with suppressed expression of c-myc, cyclins D3 and E2, and cyclin-dependent kinases CDK4 and CDK6. (wikipedia.org)
  • Here, we show that p53 suppresses EMT by repressing expression of ZEB1 and ZEB2. (researchgate.net)
  • Furthermore, miR-101 suppresses the expression of LMO3 by decreasing USF and MZF1. (oncotarget.com)
  • ADAR3 contains an arginine-rich domain, shown in pink, which binds single-stranded RNA. (biomedcentral.com)
  • The effect of miR-20b-5p on xenograft growth was investigated in vivo by transfection of a lentivirus-overexpression vector into T47D cells. (researchsquare.com)
  • MicroRNA-181 expression could be directly induced upon an activation of Wnt/β-catenin signaling, which includes Wnt10B overexpression, inhibition of GSK3β signaling by LiCl, or forced expression of β-catenin/Tcf4. (biomedcentral.com)
  • NFAT2 overexpression induced the expression of Egr2 and FasL and suppressed the phosphorylation of AKT and ERK. (researchsquare.com)
  • The sensitivity and Ca 2+ mobilization of HepG2 cells was also inhibited by NFAT2 overexpression. (researchsquare.com)
  • Overexpression of DEFB1 in IAV-infected HBEpCs led to increased NF-B expression. (cdc.gov)
  • knockdown of Pvrl1 reduced cell-surface levels of PVR but not levels of Pvr messenger RNA. (stanford.edu)
  • By profiling 92 primary hepatocellular carcinomas (HCCs) and 9 HCC cell l. (researchgate.net)
  • Immune checkpoint inhibitors are effective in the treatment of some hepatocellular carcinomas (HCCs), but these tumors do not always respond to inhibitors of programmed cell death 1 (PDCD1, also called PD1). (stanford.edu)
  • microRNA-18a Promotes Cell Migration and Invasion Through Inhibiting Dicer l Expression in Hepatocellular Carcinoma In Vitro △ [J].Chinese Medical Sciences Journal, 2017, 32(1): 34-43. (cams.cn)
  • The in vitro I/R model was established by the hypoxia/reoxygenation method using H9C2 cells. (spandidos-publications.com)
  • Instead we use an in vitro HBV-replicating liver cell model to investigate the biological role of microRNAs with a possible influence on the pathogenesis of CHB in children. (biomedcentral.com)
  • Taken together, our results demonstrate that microRNA-181s are transcriptionally activated by the Wnt/beta-catenin signaling pathway in HCC. (biomedcentral.com)
  • Transcriptome-wide research reveals that m 6 A modification may affect more than 7000 mRNAs in individual transcriptomes of mammalian cells. (biomedcentral.com)
  • A-to-I editing is a mechanism that regulates and diversifies the transcriptome, but the full biological significance of ADARs is not understood. (biomedcentral.com)
  • Eukaryotic RNA transcripts can undergo a range of post-transcriptional modifications, which increase the diversity of the transcriptome without requiring increases in genome size. (biomedcentral.com)
  • A single microRNA can have multiple downstream targets and affect a number of different networks and pathways [ 4 , 5 ]. (biomedcentral.com)
  • In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)
  • The samples were analyzed using the technology of RNA-seq and bioinformatics. (peerj.com)
  • Endothelial progenitor cells (EPCs) play a fundamental role in post-natal vascular repair, yet EPCs from different anatomic locations possess unique biological properties. (biomedcentral.com)
  • although it is derived from cells of the renal tubular epithelium, it has several histological subtypes which differ in their clinical outcome and biological features. (hindawi.com)
  • Through hypoxia-inducible factor 1 (HIF-1) which elicits various molecular events, cells are able to overcome low O2. (stanford.edu)
  • RAB18 reversed partial roles of miR-455 on cell viability and invasion in HCC. (hindawi.com)
  • Thus, the pivotal roles of miR-455 in cell viability and metastasis in HCC still needed to be explored. (hindawi.com)
  • m 6 A is the most common among various RNA modifications [ 9 ] and has critical roles in cancer pathogenesis. (biomedcentral.com)
  • Increasing evidence has indicated that long noncoding RNAs (lncRNAs) play various important roles in the development of cancers. (biomedcentral.com)
  • Emerging evidence indicates a putative role of long noncoding RNAs in regulating mitochondrial functions and metabolism. (informaticsstudies.org)
  • This article discusses the relationship between mitochondrial respiration and long noncoding RNAs and further outlines the implications of these interactions in obesity. (informaticsstudies.org)
  • Long non‑coding RNA AK055347 is upregulated in patients with atrial fibrillation and regulates mitochondrial energy production in myocardiocytes. (informaticsstudies.org)
  • Thus, A-to-I editing in both translated and untranslated regions of RNA can be biologically significant. (biomedcentral.com)
  • Objective To investigate the effects of microRNA-18a (miR-18a) on migration and invasion of hepatocellular carcinoma (HCC) cells, and its possible mechanism associated with Dicer l. (cams.cn)
  • The aim of this study is to investigate the involvement of particular miRNA in regulating chemosensitivity of HCC cells and its possible mechanism. (hku.hk)
  • Therefore, research from the entire TM rather than specific cells may be more helpful to better our understanding of the mechanism of tumorigenesis. (peerj.com)
  • Numbers shown below represent number of publications held in OncomiRDB database for Oncogenic and Tumor-Suppressive MicroRNAs. (cancerindex.org)
  • Although RNA editing has long been considered a relatively rare processing event, more recent research suggests that the vast majority of pre-mRNAs are edited [ 6 ]. (biomedcentral.com)
  • EMT is an embryologically conserved genetic program by which epithelial cells down regulate intercellular tight junctions, loose polarity, express mesenchymal markers, and manifest a migratory phenotype [ 1 ]. (intechopen.com)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • The advanced pathological grade was associated with strong TGFB1, VEGFA, and Ki67 protein expression and absent Tp53 staining. (hindawi.com)
  • Some of the same strategies already developed for delivery of siRNA for RNA interference (RNAi) also are being applied to miRNA. (genengnews.com)
  • In a PCR array analysis of 84 transcription factors, either overexpressing DEFB1 or siRNA silencing of DEFB1 expression significantly modulated the expression of signal transducer and activator of transcription 3 (STAT3). (cdc.gov)
  • Notably, berberine activated a population with immune suppressive function, defined as granulocytic‐ myeloid‐derived suppressor cell (G‐MDSC)‐like population, in the liver of mice with alleviating alcohol‐induced hepatic injury. (hku.hk)
  • Berberine remarkably enhanced the increase of G‐MDSC‐like cells in blood and liver and decreased cytotoxic T cells correspondingly. (hku.hk)
  • We analyzed the relationship between differential expression of NEK2 and hepatocellular carcinoma (HCC) patient outcomes after liver transplants. (oncotarget.com)
  • These findings suggest miR-486-5p negatively regulates NEK2, which is a critical prognostic indicator of HCC patient survival after liver transplantation. (oncotarget.com)
  • Our findings indicate that tumor associated macrophages including Kupffer cells, have a profound impact on liver cancer and I am studying the molecular basis for these effects. (stanford.edu)
  • We investigated mechanisms of resistance of liver tumors in mice to infiltrating T cells.Mice were given hydrodynamic tail vein injections of clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR-Cas9) and transposon vectors to disrupt Trp53 and overexpress C-Myc (Trp53KO/C-MycOE mice). (stanford.edu)
  • The tumor microenvironment (TM) in close contact with cancer cells is highly related to tumor growth and cancer metastasis. (peerj.com)
  • The abnormality of those functions in the organic microenvironments promoted the metastasis of HCC and initiated the biogenesis of a secondary HCC in a PM when the PM encountered the invasion of cancer cells. (peerj.com)
  • Moreover, microRNA-181 expression was inhibited upon an inactivation of Wnt/β-catenin signaling by an induction of adenomatosis polyposis coli (APC) expression or silencing β-catenin via RNA interference. (biomedcentral.com)
  • To sustain tumor growth, cancer cells must be able to adapt to fluctuations in energy availability. (researchgate.net)
  • In Trp53KO/C-MycOE mice and mice with tumors grown from Hepa1-6 cells, injection of the combination of anti-PD1 and anti-TIGIT significantly reduced tumor growth, increased the ratio of cytotoxic to regulatory T cells in tumors, and prolonged survival.PVRL1, which is up-regulated by HCC cells, stabilizes cell surface PVR, which interacts with TIGIT, an inhibitory molecule on CD8+ effector memory T cells. (stanford.edu)
  • To date, many studies have focused on WGCNA analysis of bulk or single-cell RNA (scRNA) sequencing data from tissue samples. (frontiersin.org)
  • In addition, seven putative β-catenin/Tcf4 binding sites were identified in the promoter region of the microRNA-181a-2 and microRNA-181b-2 transcripts. (biomedcentral.com)
  • A-to-I RNA editing is a post-transcriptional modification that converts adenosines to inosines in both coding and noncoding RNA transcripts. (biomedcentral.com)
  • Reverse transcription‑quantitative PCR was performed to determine the expression of miR‑132, SIRT1 and inflammatory factors. (spandidos-publications.com)
  • Breast cancer There is downregulation of miR-26a in breast cancer specimens and cell lines, and it has been shown to functionally antagonise human breast carcinogenesis. (wikipedia.org)
  • The miRNA profile between breast cancer stem cells(BCSCs, CD44+CD24-/low) and control MCF-7 breast cancer cells was obtained in a previous study. (researchsquare.com)
  • We recently identified that EpCAM (CD326)-positive cells isolated from alpha-fetoprotein (AFP)-positive HCC samples are hepatic cancer stem cells (HepCSCs). (biomedcentral.com)
  • We recently found that isolated HCC cells using a cell surface marker EpCAM (CD326) from alpha-fetoprotein (AFP) positive HCC cases are hepatic cancer stem cells (HepCSCs), where an activation of Wnt/β-catenin signaling is a major feature [ 5 ]. (biomedcentral.com)
  • DUSP6/MKP3 is a dual-specific phosphatase that regulates extracellular regulated kinase ERK1/2 and ERK5 activity, with an increasingly recognized role as tumor suppressor. (cancer-genetics.org)
  • Sequencing cell-free RNA derived from liquid biopsies has been very promising for the diagnosis of several diseases. (frontiersin.org)
  • Liquid biopsy represents a promising alternative to invasive tissue biopsies in particular for cancer diagnosis as it enables the study of cell-free (cf) nucleic acids in the blood, which includes cfDNA and various cfRNAs (e.g., protein-coding, lncRNA, microRNA, etc. (frontiersin.org)
  • To discover the microRNAs regulating this critical transition, we used 80 biopsies from invasive ductal carcinoma, 8 from ductal carcinoma in situ, and 6 from normal breast. (researchgate.net)
  • However, miR-455 promoted cell invasion and migration in triple-negative breast cancer [ 14 ]. (hindawi.com)
  • Results miR-18a inhibitor can suppress the migration and invasion of HCC cells. (cams.cn)
  • Conclusion Our findings suggest that miR-18a promotes migration and invasion of HCC cells by inhibiting Dicer l expression. (cams.cn)
  • Reducing miR-18a expression degrades migration and invasion of HCC cells. (cams.cn)
  • Transwell migration assays of HepG2 cells (A) or HepG2.2.15 cells (B) expressing miR-18a inhibitor or inhibitor negative control (inhibitor NC). (cams.cn)
  • Knocking down miR-31 levels in CB-EPCs suppressed cell migration and microtubule formation, while overexpressing miR-31 in PB-EPCs helped to recapitulate some of CB-EPC functions. (biomedcentral.com)
  • Transwell migration and invasion assays were used to examine cell migration and invasion abilities. (biomedcentral.com)