Regulate porin genes. Medical search. Frequent questions

Our work deals primarily with the outer membrane proteins, lambda receptor, the product of the lamB gene, and the major outer membrane (porin) proteins 1a and 1b, products of the ompF and ompC genes. (unibas.ch)
For example, for antibiotics to be effective against a bacterium, it must often pass through an outer membrane Porin. (miketyka.com)

These genes encode proteins involved in photosynthesis, carbon metabolism and nitrogen assimilation as well as transporter and porin proteins, protein kinases and transcription factors etc. (am-online.org)
Genes upregulated in the treated B. burgdorferi included a number of Erp genes and rplU , a 50S ribosomal protein. (frontiersin.org)
The results reveal down regulation of gene transcripts involved in general defence mechanism (e.g. antimicrobial peptide) as well as those involved in cell wall modification (e.g. glycine rich protein, xyloglucan endo transglycosylase). (biomedcentral.com)
The Parkinson's disease genes pink1 and parkin , which encode a mitochondrially targeted protein kinase, and an E3 ubiquitin ligase, respectively, participate in a key mitochondrial quality-control pathway that eliminates damaged mitochondria. (sdbonline.org)
Protein channels span these molecular castle walls and regulate the diffusional traffic of molecules trying to enter or leave the cell. (miketyka.com)
For instance, a protein that completely disappeared after dopamine treatment was VDAC2, a porin of the outer mitochondrial membrane. (uninsubria.it)
HomB protein contributes to the colonization and persistence of H. pylori , and the presence of homB genes affects the number of bacteria adhering to the host cells. (nature.com)
Mitochondrial porin , also known as the voltage-dependent anion channel (VDAC), is a multi-functional channel protein that shuttles metabolites between the mitochondria and the cytosol and implicated in cellular life and death decisions. (sdbonline.org)
An optimal loading control should detect a globally expressed protein that is not differentially regulated in normal and disease states. (novusbio.com)

This study describes the proteomic responses of two carbapenem-resistant P. aeruginosa strains of high-risk clones ST235 and ST395 to subminimal inhibitory concentrations (sub-MICs) of meropenem by identifying differentially regulated proteins and pathways. (unit.no)
Exposure of strains to sub-MICs of meropenem resulted in hundreds of differentially regulated proteins, including β-lactamases, proteins associated with transport, peptidoglycan metabolism, cell wall organization, and regulatory proteins. (unit.no)
EnvZ is a histidine kinase that senses changes in medium osmolarity and signals its response regulator OmpR to bind to OmpF and OmpC porin promoters to differentially modulate their transcription. (mechanobio.info)

2004). A novel outer-membrane anion channel (porin) as part of a putatively two-component transport system for 4-toluenesulphonate in Comamonas testosteroni T-2. (tcdb.org)
Small molecules can pass through Porins, or voltage-dependent anion selective channels or VDAC. (notesbard.com)

EnvZ responds to cytoplasmic signals that arise from changes in the extracellular milieu, and OmpR acts canonically (requiring phosphorylation) to regulate the porin genes and noncanonically (without phosphorylation) to activate the acid stress response. (utmb.edu)
Both PhoQ/PhoP and EnvZ/OmpR regulate SsrA-SsrB. (mechanobio.info)

This study demonstrates that debcl and the pro-fission gene drp1 are necessary downstream of buffy to trigger a mitochondrial fragmentation during rbf1 -induced apoptosis. (sdbonline.org)

The structural genes for the porin proteins, 1a and 1b, are regulated at the transcriptional level by the ompB locus. (unibas.ch)
A study of Escherichia coli physiology during growth on glycerol was performed combining transcriptional-proteomic analysis as well as kinetic and stoichiometric evaluations in the strain JM101 and certain derivatives with important inactivated genes. (biomedcentral.com)
Characterization of DNA binding, transcriptional activation, and regulated nuclear association of recombinant human NFATp. (colorado.edu)

Our results show that different cyanobacterial species/ecotypes may encode diverse sets of genes responsible for the utilization of various sources of phosphorus, ranging from inorganic phosphate, phosphodiester, to phosphonates. (biomedcentral.com)
cMonkey integrates powerful de novo motif detection to identify conditionally co-regulated sets of genes. (systemsbiology.net)

Because the genes are typically encoded on large plasmids that contain additional antimicrobial resistance genes, therapeutic options are limited ( 3 ). (cdc.gov)
Our goal was to examine gene transcription by B. burgdorferi following doxycycline treatment in an effort to identify both persister-associated genes and possible targets for antimicrobial intervention. (frontiersin.org)
It causes high mortality and morbidity especially in low- and middle-income countries where access to diagnostics is limited and antimicrobial prescription and intake remain inadequately regulated. (who.int)
Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)

There include the genes ecoding AC and PDE cAMP acceptors (CRP), which are transcription factors activated by this nucleotide and genes encoding proteins playing an important role in different physiological processes whose promoters have the CRP binding sequence. (am-online.org)
A subset of cell wall modification and stress related genes was further assessed by quantitative reverse transcription PCR at late stages of mycorrhizal development coinciding with Hartig net formation. (biomedcentral.com)
More interestingly, in three cyanobacterial genomes where no sphR gene is encoded, our results show that there is virtually no functional SphR binding site, suggesting that transcription regulators probably play an important role in retaining their binding sites. (biomedcentral.com)
All the genes whose transcription is regulated by PhoB in a bacterium are collectively called a Pho regulon . (biomedcentral.com)
PTS not only transports specific sugars but also in the absence of its substrates, stimulates, through adenylate cyclase (Cya), the production of c AMP which in turn activates the transcription of many c AMP-CRP dependent catabolic genes, including those involved in glycerol utilization [ 1 ]. (biomedcentral.com)
Genome regulation encompasses all facets of gene expression, from the biochemical modifications of DNA, to the physical arrangement of chromosomes and the activity of the transcription machinery. (mechanobio.info)
To validate experimentally the hypothesis of the NF-κB involvement, we performed a luciferase gene reporter assay that revealed a downregulation of the transcription factor activation by α-synuclein increased expression and its complete quenching by dopamine exposure. (uninsubria.it)

E. coli possess a chromosomal gene that encodes for an AmpC β-lactamase. (cdc.gov)
Unlike in E. coli , some cyanobacterial genes that are directly involved in phosphorus assimilation seem to not be under the regulation of the regulator SphR (orthologue of PhoB in E coli . (biomedcentral.com)
The expression of metabolic genes, in particular the overexpression of pykA pckA, gltA fumABC sdh mdh and acnA genes and the downregulation of the ackA gene, has been reported for E. coli growing on glycerol [ 7 ]. (biomedcentral.com)
In biofilms, UDP-glucose dehydrogenase with a major role in exopolysaccharide production and the non-fimbrial adhesin YapH involved BEZ235 in adherence were over-expressed, while a polynucleotide phosphorylase that was demonstrated to negatively control biofilm formation in E. coli was down-regulated. (hdac-inhibitors.com)

Strain CCUG 51971 showed upregulation of intrinsic β-lactamases and VIM-4 carbapenemase, while CCUG 70744 exhibited a combination of upregulated intrinsic β-lactamases, efflux pumps, penicillin-binding proteins and downregulation of porins. (unit.no)

Bacteria can develop resistance to the antibiotic by mutating the gene that encodes the Porin , the antibiotic is then excluded from passing through the outer membrane. (miketyka.com)
The S. mutans mntE gene encodes a manganese efflux transporter. (middlebury.edu)

The homologous sRNA in S. enterica was shown to regulate synthesis of major porin OmpD. (wikipedia.org)
In these organisms the presence of cAMP synthesis and degradation enzymes (AC and PDE, respectively) was demonstrated and genes encoding proteins involved in signal transduction pathway of cAMP were identified. (am-online.org)
Over all, this study indicates that aceE gene plays a significant role in the mycolic acid synthesis and affects the colony morphology, biofilm formation of M. smegmatis and bacteria invasion of macrophage. (biomedcentral.com)

A large number of genes coding for enzymes predicted to synthesize and degrade 3′-5′-cyclic diguanylic acid (c-di-GMP) is found in most bacterial genomes and this dinucleotide emerged as an intracellular signal-controlling bacterial behaviour. (inrs.ca)
En combinant une approche in silico et la validation in vitro de l'activité des protéines, nous avons récemment mis en évidence des propriétés structurales et la sélectivité de substrat de ces enzymes. (ipbs.fr)

Bacteria respond to changing environments by altering gene expression. (mechanobio.info)
Two-component regulatory systems (TCRS) are important mediators of signal transduction that enable bacteria to detect physical and/or chemical changes and then relay this signal through the cytoplasm to the bacterial nucleoid, where modulation of gene expression occurs. (mechanobio.info)

OmpW of Salmonella enterica serovar Typhimurium has been described as a minor porin involved in osmoregulation, and is also affected by environmental conditions. (uandes.cl)
The two component TCRS SsrA-SsrB in Salmonella contributes to virulence by de-repressing the H-NS mediated silencing of SPI-2 genes. (mechanobio.info)

An association between high levels of c-di-GMP and antibiotic resistance may be expected because c-di-GMP regulates biofilm formation and this mode of growth leads to enhanced antibiotic resistance. (inrs.ca)

In addition, the Mla system was recently shown to regulate outer membrane vesicle formation 16 . (nature.com)

A limited study had indicated that in Sinorhizobium meliloti the YbeY ortholog regulates the accumulation of sRNAs as well as the target mRNAs, raising the possibility that YbeY may play a previously unrecognized role in bacterial sRNA regulation. (biomedcentral.com)

In addition, altered expression of outer membrane proteins constituting porins can also contribute to cephamycin resistance ( 6 ). (cdc.gov)
strain CCUG 70744 carries no known acquired carbapenem-resistance genes and exhibits 'non-classical' carbapenem-resistance. (unit.no)
Loss of ybeY function, which results in a marked resistance to HU, had global affects on sRNA-mediated gene expression. (biomedcentral.com)
In addition, long-read sequencing of one representative XDR ST235 isolate identified an integron carrying multiple resistance genes (including bla VIM-2), with differences in gene composition and synteny from the P. aeruginosa class 1 integrons described previously. (who.int)
This report presents the status of AMR in Africa by analysing the main types of resistance and the underlying genes where possible. (who.int)

Main efflux pumps or β-lactamase genes did not show altered mRNA levels in P. aeruginosa strains with modified different c-di-GMP concentrations. (inrs.ca)
The most extensively characterized class of bacterial sRNAs are the trans -encoded sRNAs that are encoded distant from the genes for their mRNA targets and that typically have only limited complementarity (10-30 nt) [ 2 ]. (biomedcentral.com)

2015). Comparative genomics reveals new candidate genes involved in selenium metabolism in prokaryotes. (tcdb.org)
The overexpression of several carbon scavenging, acetate metabolism genes and the absence of acetate accumulation occurred in JM101 cultures growing on glycerol. (biomedcentral.com)
We hypothesized that changes in morphology may indicate changes in cell wall metabolism and identified an aceE gene mutant ( aceE -mut) which presented a deficient colony morphology on 7H10 agar by screening transposon mutagenesis in Mycolicibacterium smegmatis , basonym Mycobacterium smegmatis ( M. smegmatis ). (biomedcentral.com)

P. aeruginosa post-transcriptionally regulates the amounts of five porins in response to c-di-GMP, including OprD, responsible for imipenem uptake. (inrs.ca)

2011). PigS and PigP regulate prodigiosin biosynthesis in Serratia via differential control of divergent operons, which include predicted transporters of sulfur-containing molecules. (tcdb.org)

The observed overexpression of poxB , pta , acs genes, glyoxylate shunt genes ( aceA, aceB , glcB and glcC ) and actP , suggested a possible carbon flux deviation into the PoxB, Acs and glyoxylate shunt. (biomedcentral.com)

2011). Evidence-based annotation of gene function in Shewanella oneidensis MR-1 using genome-wide fitness profiling across 121 conditions. (tcdb.org)

Attributed to the failure of neurons to clear dysfunctional mitochondria , loss of gene expression leads to loss of nigrostriatal neurons. (sdbonline.org)
One class of these molecular gatekeepers are the Porins, beta-barrel proteins that are situated in the outer membranes of cells or organelles such as human mitochondria. (miketyka.com)

Most common loading control targets are highly expressed constitutive genes required for basic cellular processes and cellular vitality (housekeeping genes). (novusbio.com)

Porins can be chemically selective, they can transport only one group of molecules, or may be specific to one molecule. (miketyka.com)
S-layer, adhesin, and flagellin gene expression), and selective microbial competition (e.g., bacteriocin gene expression) between the slow and fast oxygenation treatments during microbial Fe(III) reduction. (biomedcentral.com)

Proteomic and enzymatic assay studies, in which cells were grown on a complete medium (Luria broth) plus glycerol, reported overexpression of the fbp gene and at lower levels aceBA operon [ 8 ]. (biomedcentral.com)
Other bacterial sRNAs, referred to as cis -encoded sRNAs, are present in close proximity to their targets, such as upstream, opposite of the 5′ UTR of the target, or between two genes in an operon [ 2 ]. (biomedcentral.com)

The phosphoryl group is transferred onto an aspartate of the response regulator, which activates the regulator and alters its output, usually resulting in a change in gene expression. (utmb.edu)

This study aimed to identify the functional role of aceE gene in cell wall biosynthesis in M. smegmatis . (biomedcentral.com)
Mutagenesis of cysQ (Rv2131c) was initially unsuccessful, but was possible when a porin-like gene of Mycobacterium smegmatis was expressed, and also by gene switching in the merodiploid strain. (biomedcentral.com)
Experiments to obtain a mutant in the presence of a second copy of impC containing an active-site mutation, in the presence of porin-like gene of M. smegmatis , or in the absence of inositol 1-phosphate synthase activity, were also unsuccessful. (biomedcentral.com)

The number of genes and the amplitude of their expression vary in time but seem to be more important at the very early stages of development, soon after contact is made. (biomedcentral.com)
Small regulatory RNAs (sRNAs) play key roles in modulating gene expression in both prokaryotes and eukaryotes. (biomedcentral.com)
The directed expression of alpha-synuclein , a gene contributing to inherited forms of Parkinson disease (PD), in the dopaminergic neurons (DA) of flies provides a robust model of PD complete with the loss of neurons and accompanying motor defects. (sdbonline.org)
Metatranscriptomic analysis and 57 Fe Mössbauer spectroscopy were used to investigate changes in Fe(III)-reducer gene expression and Fe(III) crystallinity, respectively. (biomedcentral.com)
Transcripts binning to the genomes of Fe(III)-reducers Anaeromyxobacter , Geobacter , and Pelosinus indicated significant differences in extracellular electron transport (e.g., multiheme cytochrome c , multicopper oxidase, and type-IV pilin gene expression), adhesion/contact (e.g. (biomedcentral.com)
Additionally, the metatranscriptomic data indicate that Methanobacterium was significantly more active in the reduction of CO 2 to CH 4 and in the expression of class(III) signal peptide/type-IV pilin genes following repeated fast oxygenation compared to slow oxygenation. (biomedcentral.com)

Currently the sequences of 29 complete genomes of different species of cyanobacteria (and their strains) are known in which different genes connected with cAMP pathway have been identified. (am-online.org)
Current studies of cAMP signaling pathway in cyanobacteria may indicate that it is not a universal pathway regulating cell functioning in the genomes of some cyanobacteria AC, PDE, CRP genes and genes whose activity is regulated by CRP were not detected. (am-online.org)

Moreover, the bioinformatic approach found out the enriched Gene Ontology (GO) categories, allowing us to draw attention to the NF-κB pathway. (uninsubria.it)

In agreement, no acetate was detected when growing on glycerol, this strain was also capable of glycerol and acetate coutilization when growing in mineral media and derivatives carrying inactivated poxB or pckA genes, accumulated acetate. (biomedcentral.com)
The analysis of mycolic acid (MA) using LC-MS indicated deficiency of alpha-MA and epoxy-MA in aceE -mut strain whereas complementation of the aceE -mut with a wild-type aceE gene restored the composition of MA. (biomedcentral.com)
In the present study, we found a mutant strain in which the aceE gene, encoding the E1 component of PDH, was inactivated by Himar1 transposon insertion ( aceE -mut). (biomedcentral.com)

Antagonistic roles of ubiquitin ligase HEI10 and SUMO ligase RNF212 regulate meiotic recombination. (middlebury.edu)
Loss of porin function was achieved through exploitation of RNAi while derivative lines were generated by homologous recombination and tested by PCR. (sdbonline.org)

The main module page also shows the location of these motifs within the upstream sequences of the module member genes. (systemsbiology.net)
cMonkey tries to identify two motifs per modules in the upstream sequences of the module member genes. (systemsbiology.net)

Using techniques of gene fusion, we have demonstrated that information specifying the cellular location of the lambda receptor is contained within the lamB gene. (unibas.ch)

Interestingly, some proteins related to energy production, such as ATP-synthase were down-regulated selleck kinase inhibitor in biofilms. (hdac-inhibitors.com)

The Hartig net is the site of metabolite exchange between fungal and root cells[ 1 ] The ontogenesis of a functional ECM symbiosis requires finely regulated cross talk in time and space between plant and fungal genetic programs involved in sensing the environment, and in cell-to-cell communication. (biomedcentral.com)

Usually, low amounts of β-lactamases are produced because the AmpC gene is regulated by a weak promoter and a strong attenuator. (cdc.gov)

The SoxS site is in the forward orientation and its location suggests that the ompW gene has a class I SoxS-dependent promoter. (uandes.cl)

The porin proteins allow free passage for various molecules into intermembrane space. (notesbard.com)

Again using techniques of gene fusion, we have shown that the signal sequence alone is not sufficient to cause export of beta-galactosidase from the cytoplasm. (unibas.ch)

Glycolipids, porins and lipoarabinomannan as well as its variants, which are anchored to the cell membrane by diacylglycerol, are also essential components [ 2 ]. (biomedcentral.com)
The free calcium concentrations in cell can regulate many reactions and important for signal transduction. (notesbard.com)
Only two Bcl-2 family genes have been found in Drosophila melanogaster including the pro-cell survival, human Bok-related orthologue, Buffy. (sdbonline.org)
Taken all together this demonstrates a role for Buffy, a Bcl-2 pro-cell survival gene, in neuroprotection. (sdbonline.org)

showed that prokaryotic members of the family outnumber the eukaryotic members substantially, and in prokaryotes, but not eukaryotes, extensive lateral gene transfer occurred during family evolution. (tcdb.org)

On the other hand, SphR binding sites are found for genes known to play important roles in other biological processes. (biomedcentral.com)

Organisms7

Chemicals and Drugs11

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes4

Technology, Industry, Agriculture1

Information Science2

Outer membrane porin2

Protein9

Differentially3

Anion2

OmpR2

Mitochondrial1

Transcriptional3

Sets of genes2

Antimicrobial4

Transcription7

Coli4

Downregulation1

Encodes2

Synthesis3

Enzymes2

Bacteria2

Salmonella2

Biofilm formation1

Shown to regulate1

Ortholog1

Resistance5

MRNA2

Metabolism3

OprD1

Operons1

Glyoxylate1

Shewanella1

Mitochondria2

Constitutive1

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Regulator1

Smegmatis3

Expression6

Genomes2

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Motifs2

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