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OsteoblastsCell DifferentiationOsteogenesisCore Binding Factor Alpha 1 SubunitBone Morphogenetic Protein 2Cell ProliferationCalcification, PhysiologicOsteocalcinCells, CulturedAlkaline PhosphataseSignal TransductionSkullTranscription FactorsBone and BonesCell LineBone Morphogenetic ProteinsGene Expression RegulationMesenchymal Stromal CellsIntegrin-Binding SialoproteinBone DevelopmentStem CellsRNA, MessengerGene Expression Regulation, DevelopmentalOsteoclastsCell DivisionCell LineageReverse Transcriptase Polymerase Chain ReactionSmad ProteinsMice, Inbred C57BLOsteocytesMice, KnockoutMice, TransgenicBone Marrow CellsTransforming Growth Factor betaGene ExpressionBlotting, WesternHomeodomain ProteinsRANK LigandDNA-Binding ProteinsBone RemodelingPromoter Regions, GeneticImmunohistochemistryTranscription, GeneticWnt ProteinsAdipogenesisMolecular Sequence DataBone MatrixPhenotypeBase SequenceApoptosisBone Morphogenetic Protein 6TransfectionEmbryonic Stem CellsSex DifferentiationUp-Regulationbeta CateninDown-RegulationCell Line, TumorTime FactorsWnt3A ProteinStromal CellsRNA, Small InterferingOsteopontinWnt3 ProteinTrans-ActivatorsAdipocytesModels, BiologicalBone ResorptionCell CycleChondrocytesAntigens, DifferentiationIn Situ HybridizationMyoblastsCell Culture TechniquesSmad1 ProteinIntercellular Signaling Peptides and ProteinsNeoplasm ProteinsGene Expression Profiling3T3 CellsTretinoinOsteoprotegerinCell SurvivalCollagen Type IEmbryo, MammalianSkeletonTumor Cells, CulturedPhosphorylationHedgehog ProteinsProtein BindingMesodermBone Diseases, MetabolicInhibitor of Differentiation ProteinsMutationBone Morphogenetic Protein 4Flow CytometryActivating Transcription Factor 4DNA PrimersWnt Signaling PathwayNuclear ProteinsBiological Markers
OsteogenicMSCsAdipocytesMaturationChondrocytesApoptosisTranscriptionalOsteocytesDifferentiateMesenchymalGenesChondrocyte proliferationInhibitionCellOsteogenesisRegulationRole in the differentiationMineralizationPhysiologicalSignaling pathwayStimulatesMigrationCellsHuman osteoblastsMechanismsInduceInhibitVivoVitroExtracellularOsteoclastIntracellularTranscription factorGene expressionTrabecularAdipocyteOsteoclastsDeficiencyFunctionsOsteoidSelectivelySkeletalImmuneIntramembranousHomeostasisOxidationFunction
Osteogenic21
  • We subsequently discuss how β-TCP can regulate osteogenic processes to aid bone repair/healing, namely osteogenic differentiation of mesenchymal stem cells, formation of blood vessels, release of angiogenic growth factors, and blood clot formation. (frontiersin.org)
  • However, the primary mechanisms regulating osteogenic differentiation are controversial. (nature.com)
  • As primary sources of osteoblasts, MSCs show osteogenic functions that are closely associated with bone homeostasis and development. (nature.com)
  • Variations in osteogenic potential have been demonstrated between marrow and periosteal osteoblasts from the same individual [ 7 ]. (biomedcentral.com)
  • Multipotential differentiation of MSCs into adipogenic, osteogenic, and chondrogenic cell lineages. (hindawi.com)
  • The pathogenesis of myelodysplastic syndrome (MDS) may be related to the abnormal expression of microRNAs (miRNAs), which could influence the differentiation capacity of mesenchymal stem cells (MSCs) towards adipogenic and osteogenic lineages. (techscience.com)
  • Transfection of MDS-MSCs with the miR-103-3p mimic improved osteogenic differentiation and decreased adipogenic differentiation in vitro, while inhibition of miR-103-3p showed the opposite results in NOR-MSCs. (techscience.com)
  • The miR-103-3p mimics may boost MDS-MSCs osteogenic differentiation while weakening lipid differentiation, thereby providing possible target for the treatment of MDS pathogenesis. (techscience.com)
  • osteogenic differentiation. (healthyconnectionsinc.com)
  • The capacity of Cisd2-deficient miPSCs for differentiation into multiple lineages, particularly osteogenic lineages, was also investigated. (healthyconnectionsinc.com)
  • Embryoid body-mediated osteogenic differentiation For embryoid body (EB) formation, miPSCs were dissociated into a single cell suspension using Notch1 0.25% trypsin-EDTA and plated onto nonadherent bacterial culture dishes at a density of 2? (healthyconnectionsinc.com)
  • Hypoxia and the hypoxia-inducible factor (HIF) transcription factor regulate angiogenic-osteogenic coupling and osteoclast-mediated bone resorption. (ox.ac.uk)
  • p38 pathway plays a very important role in the osteogenic differentiation of mesenchymal stem cells (MSCs). (cusabio.com)
  • Inhibition of the p38 MARK pathway down-regulates the activity of protein kinase C ( PKC ), which plays an important role in osteogenic differentiation of cells. (cusabio.com)
  • Among them, Runx2 is a key target gene affecting osteogenic activity, and Cbfa1 regulates the differentiation of MSCs into osteoblasts at the transcriptional level. (cusabio.com)
  • The results showed that MSC did not alter their osteogenic differentiation capacity, surface marker profile, and the expression profiles of MSC during expansion. (biomedcentral.com)
  • Microarray analysis of MSC during osteogenic differentiation identified three candidate genes for further examination and functional analysis: ID4, CRYAB, and SORT1. (biomedcentral.com)
  • Finally we analyzed the gene expression profiles of MSC during osteogenic differentiation. (biomedcentral.com)
  • Pl/Mb-MSCs shown higher osteogenic differentiation potential than BM-MSC when their response to osteoactivin was examined. (biomedigs.org)
  • It was described that hedgehog proteins right act on osteogenic precursor cells and osteoblasts to stimulate osteogenic differentiation [7]. (acthreceptor.com)
  • These stories advise tha 16926152 t Shh has a osteogenic and osteoclastogenic activity in osteoblast cells [11], but the downstream signaling of SHH in fracture therapeutic has not been determined. (acthreceptor.com)
MSCs16
  • Mesenchymal stem cells (MSCs) show trilineage differentiation because these cells differentiate into osteocytes, chondrocytes and adipocytes. (nature.com)
  • Adipose-derived MSCs, in particular, pose an attractive option for cell-based therapy due to their relatively decreased morbidity during isolation and potential for expansion and differentiation [ 12 ]. (hindawi.com)
  • Thus, the expression of miR-103-3p decreases in MDS BM plasma and MDS-MSCs, significantly impacting MDS-MSCs differentiation. (techscience.com)
  • MSCs have a lower ability to differentiate into osteoblasts but a higher ability to differentiate into adipocytes, which could be linked to myeloid malignancies ( Woods and Guezguez, 2021 ). (techscience.com)
  • Many studies have shown that MSCs possess immunosuppression abilities, such as the capacity to inhibit proliferation and functions of many immune cell types. (weizmann.ac.il)
  • It has been established that MSCs also express TLRs and that their differentiation is modulated by TLR ligands. (weizmann.ac.il)
  • The ERK-MAPK signaling pathway plays a role in the differentiation of mesenchymal stem cells (MSCs) into adipocytes [1] [2] . (cusabio.com)
  • MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)
  • Transcriptomic evaluation and cytokine arrays confirmed wide similarity between placenta- and membrane-derived MSCs in support of discrete distinctions with BM-MSCs with enrichment of systems involved in bone tissue differentiation. (biomedigs.org)
  • We previously confirmed that OA induces equivalent osteoblastic differentiation than BMP2 in mice MSC recommending that OA could be a book osteoinductive agent [29 31 32 Within this research we optimized the isolation of placental and amniotic membrane MSC and likened their proliferative and differentiation potential to BM-MSCs. (biomedigs.org)
  • We further looked into and confirmed U 73122 U 73122 that OA sets off osteoblastic differentiation in individual MSCs which the differentiation was a lot more essential in fetal MSCs when compared with BM-MSCs. (biomedigs.org)
  • than BM-MSCs to differentiate into osteoblasts. (biomedigs.org)
  • Mesenchymal stem cells (MSCs), which are activated by secreted, active transforming growth factor beta 1 (TGFβ) to migrate to bone-resorptive sites and differentiate into osteoblasts (see below). (biomedcentral.com)
  • Pre-osteoblasts, a heterogeneous population of cells, including those transitioning from MSCs to mature osteoblasts, which express the transcription factor runt related transcription factor 2 (RUNX2) , a key player in the osteogenesis process. (biomedcentral.com)
  • Human orofacial bone-derived mesenchymal stem cells (OMSCs) showed distinct differentiation traits from mesenchymal stem cells (MSCs) derived from long bones, mouse OMSCs have not been isolated due to technical difficulties, which in turn precludes using mouse models to study orofacial diseases. (ca.gov)
  • Furthermore, we found that OMSCs are distinct from long bone MSCs with respect to regulating T-lymphocyte survival and proliferation. (ca.gov)
Adipocytes5
Maturation3
  • Pulsed electromagnetic fields promote osteoblast mineralization and maturation needing the existence of primary cilia med. (emf-portal.org)
  • Additionally, we were able to reconstruct the three developmental phases during osteoblast differentiation: proliferation, matrix maturation, and mineralization, and illustrate the activation of the SMAD signaling pathways by TGF-β2 and BMPs. (biomedcentral.com)
  • They regulate cell proliferation, differentiation, motility and survival from the embryonic phase until the adult phase and especially promote differentiation of myoblasts into osteoblasts and their maturation. (researchensemble.com)
Chondrocytes2
  • 21 young (8 weeks of age) and 15 adult (16 weeks of age) C57/BL/6 female mice were used{rodents retain a cartilagenous template post growth plate cessation however if LSJL still involves differentiation of stem cells into chondrocytes then it can still work in human adult mice it'll just be harder as mice already have a cartilagenous matrix to work with. (heightquest.com)
  • In endochondral bone formation, parathyroid hormone-related protein (PTHrP)-expressing RZSCs are the source of growth plate chondrocytes, which give rise to bone-forming osteoblasts as well as bone marrow skeletal stem cells 1 . (nature.com)
Apoptosis5
  • Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. (nih.gov)
  • Many genes encoding proteins that regulate cell proliferation, differentiation, and apoptosis are modulated in part by Vitamin D [1]. (xps.org)
  • APS regulated osteocyte autophagy and apoptosis via SP1/miR-200b-3p axis and activated Wnt/β-catenin signaling, thereby alleviating SONFH, shedding new insights for therapy of SONFH. (biomedcentral.com)
  • Recently, APS was reported to improve SONFH via regulating cell autophagy and apoptosis [ 9 ]. (biomedcentral.com)
  • Paracrine effect of bone marrow mesenchymal stem cells on proliferation, apoptosis, and alpha-actin-2 expression in hepatic stellate cells. (geneticsmr.com)
Transcriptional3
  • Equally important for developmental transcriptional control, histone gene expression is repressed when differentiation is initiated. (elsevierpure.com)
  • MicroRNAs (miRNAs or miRs) are small non-coding RNAs that participate in diverse cellular processes and negatively regulate gene expression at the post-transcriptional level by binding with 3′-untranslated regions (3′-UTRs) ( 2 - 4 ). (spandidos-publications.com)
  • PSCs are considered to exert their effects exclusively in intramembranous bone formation, since Ctsk -Cre-mediated deletion of Sp7 , an essential transcriptional factor for osteoblast differentiation, impacted intramembranous but not endochondral bone formation 3 . (nature.com)
Osteocytes8
Differentiate2
  • Human mesenchymal stem cells (MSC) with the capacity to differentiate into osteoblasts provide potential for the development of novel treatment strategies, such as improved healing of large bone defects. (biomedcentral.com)
  • For bone formation to happen, osteoblast cells need to proliferate and migrate from the bone marrow compartment to bone surfaces, the place they adhere, differentiate, and deposit the bone matrix concurrently with bone and bony callus resorption by osteoclasts [1]. (acthreceptor.com)
Mesenchymal2
  • Additionally, decreased activity and abnormal differentiation of bone marrow mesenchymal stem cells may be an important mechanism in the occurrence and development of SONFH [ 5 ]. (biomedcentral.com)
  • At the cellular level, the mesenchymal stem cell pool in the bone marrow niche shows a biased differentiation into adipogenesis at the cost of osteogenesis. (biomedcentral.com)
Genes4
  • Composed of multiple constituent enhancers, super enhancers (SEs) are powerful cis-regulatory elements that identify genes that ensure sequential differentiation. (nature.com)
  • In proliferating cells transcription of the histone genes is up-regulated at the onset of S phase. (elsevierpure.com)
  • A cell proliferation assay, alkaline phosphatase (ALP) activity assay, alizarin red staining, and expression analysis of osteoblastic genes were carried out to assess MC3T3-E1 cell proliferation and osteoblastic differentiation. (degruyter.com)
  • The contributions of nuclear structure as it regulates and is regulated by the progressive developmental expression of cell growth and bone cell related genes will be examined. (umassmed.edu)
Chondrocyte proliferation1
  • Indian hedgehog (IHH), one of its members, stimulates chondrocyte proliferation and osteoblast differentiation. (umontreal.ca)
Inhibition1
Cell21
  • Proliferation is a requirement during the initial stages of cell and tissue specialization. (elsevierpure.com)
  • The effects of exposure of rat calvarial osteoblasts to a pulsed 50 Hz magnetic field on cell proliferation and differentiation as well as and the role of primary cilia in this context should be investigated. (emf-portal.org)
  • A vast amount of in vitro work has followed since and osteoblastic cell lines have been used as models for understanding numerous characteristics such as differentiation, cytokine and hormone regulation, bone matrix synthesis and the molecular mechanism of bone disease. (biomedcentral.com)
  • Knockdown of MSI2 inhibited VSMC proliferation, cell-cycle, and migration. (bvsalud.org)
  • However, whether Cisd2 affects mitochondrial function to further modulate stem cell biology and cellular differentiation during early development remains unclear. (healthyconnectionsinc.com)
  • Cell differentiation is the process where a cell changes from one type to many different types. (cusabio.com)
  • In the process of cell differentiation, there are differences in morphological structure and physiological function. (cusabio.com)
  • Cell differentiation occurs throughout life. (cusabio.com)
  • MAPK can cause the activation of transcription factors in the nucleus, participate in the process of signal transmission from the cell surface to the nucleus, and regulate cell proliferation and differentiation. (cusabio.com)
  • The JNK signaling pathway can affect a variety of life processes such as cell growth, cell differentiation, and cell death. (cusabio.com)
  • In addition, miR‑205 overexpression reduced and miR‑205 depletion enhanced PC cell proliferation and migration in vitro. (spandidos-publications.com)
  • Notably, overexpression of RUNX2 partially reversed the inhibitory effect of miR‑205 on PC cell proliferation and migration in vitro. (spandidos-publications.com)
  • A number of studies have demonstrated that altered expression of miRs serves critical roles in human cancers by directly regulating cell behaviors ( 5 - 7 ). (spandidos-publications.com)
  • We found that I. sinicola extract (ISE) increased cell proliferation in a dose-dependent manner. (degruyter.com)
  • Despite growing experience and knowledge concerning human MSC and their use in cell-based strategies, the molecular mechanisms that govern MSC self-renewal, expansion and multilineage differentiation are not well understood and remain an active area of investigation. (biomedcentral.com)
  • MC3T3-E1 cell proliferation was enhanced by P3 via ERK1/2 activation. (allergy-link.com)
  • While acknowledging progress in the other areas mentioned, this work will focus on the current debates concerning sourcing, MSC alterations of angiogenesis, cell differentiation/stimulation, and strategies to improve MSC differentiation. (biomedcentral.com)
  • 4) Are alterations in gene expression and cell phenotypic properties in transformed osteoblasts and osteosarcoma cells reflected by modifications in nuclear matrix proteins? (umassmed.edu)
  • Osteoactivin (OA) has the capacity to regulate cell proliferation adhesion differentiation and synthesis of extracellular matrix protein in a variety of cell types [20-30]. (biomedigs.org)
  • Sonic 125256-00-0125B11 hedgehog (Shh) is a forty five-kDa potent signaling protein that regulates the proliferation, differentiation, and cellular patterning throughout a extensive selection of cell types [2,3]. (acthreceptor.com)
  • Focal adhesion kinase (FAK) is a one hundred twenty five-kD non-receptor tyrosine kinase that plays a main position in mediating sign transduction by integrins, as effectively as by growth aspect receptors, in the regulation of cell adhesion, migration, proliferation, and differentiation in a range of mobile varieties [12,thirteen,fourteen]. (acthreceptor.com)
Osteogenesis2
Regulation7
  • In most biological systems the down-regulation of proliferation is necessary for initiation of key steps in the differentiation process. (elsevierpure.com)
  • Therefore, we investigated the regulation of Dlx3 and found that ER-α is a positive regulator of Dlx3 transcription in BMP2-induced osteoblast differentiation. (molcells.org)
  • The p38 signaling pathway regulates the differentiation direction of stem cells, which may be related to the regulation of the stability of p53 protein [3] [4] . (cusabio.com)
  • In addition, the ERK5/BMK1 pathway, an atypical MAPK pathway, is involved in the regulation of adipocyte differentiation and fat metabolism. (cusabio.com)
  • The principal theme of this prospect will be the contribution of nuclear structure to the regulation of gene expression as functionally related to development and maintenance of the osteoblast phenotype during establishment of bone tissue-like organization. (umassmed.edu)
  • OA messenger ribonucleic acidity (mRNA) and proteins are portrayed by individual and rodent osteoblasts [29 30 OA down-regulation reduces osteoblast differentiation and function [31]. (biomedigs.org)
  • Depending on these effects, we propose that COX might possibly perform a function while in the Akt mediated regulation of osteoblasts proliferation. (mirnasynthesis.com)
Role in the differentiation1
  • These results demonstrate that Cdk1 plays an essential role in the differentiation from osteoblast to osteocyte and the acquisition and maintenance of bone mass. (lu.se)
Mineralization1
  • of ALP, COL-1, OCN, consequently promoting the differentiation and mineralization of MC3T3-E1 cells via activation of p38 MAPK pathway. (allergy-link.com)
Physiological3
  • We will consider signalling mechanisms that integrate the complex and interdependent responsiveness to physiological mediators of osteoblast proliferation and differentiation. (umassmed.edu)
  • Despite the fact that a preceding research noticed that both COX and COX levels are enhanced following mechanical stimuli inside the osteoblastic and osteoclastic lineages , the physiological part of constitutively expressed COX in osteoblasts hasn't been reported. (mirnasynthesis.com)
  • Dependant on these previous final results plus the finding of this in vivo review, it is hugely attainable that constitutively expressed COX plays a substantial physiological role in regulating osteoblast proliferation. (mirnasynthesis.com)
Signaling pathway4
Stimulates1
Migration3
  • Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. (nih.gov)
  • 15]. A modern report showed that the phosphorylation of FAK is crucial for bone development and osteoblast migration [16]. (acthreceptor.com)
  • Phosphorylated FAK at the Tyr397 web site is a critical issue for the adhesion and migration of osteoblast in fracture healing [19]. (acthreceptor.com)
Cells18
  • It maintains normal hematopoietic function by interacting with hematopoietic stem cells (HSCs) to control their proliferation and differentiation ( Morrison and Scadden, 2014 ). (techscience.com)
  • For example, humans are derived from fertilized eggs, and this process involves the differentiation of embryonic stem cells. (cusabio.com)
  • Abnormal differentiation of cells can lead to cancerous cells. (cusabio.com)
  • This pathway can also affect the proliferation and differentiation of red blood cells. (cusabio.com)
  • The aim of this study was to investigate the effects of Ishige sinicola , a brown alga, on osteoblast differentiation through the activation of the bone morphogenetic protein 2 (BMP-2)/runt-related transcription factor 2 (Runx2) signalling pathway in MC3T3-E1 cells. (degruyter.com)
  • We monitored these cells during their expansion ex vivo with respect to proliferation kinetics, surface marker profile and differentiation potential. (biomedcentral.com)
  • The response of target cells is further regulated by their microenvironment, involving the extracellular matrix, which may be modified by MSC-produced matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs. (biomedcentral.com)
  • There is a striking representation of nuclear matrix proteins unique to cells, tissues as well as developmental stages of differentiation, and tissue organization. (umassmed.edu)
  • Together with selective association of regulatory molecules with the nuclear matrix in a growth and differentiation-specific manner, there is a potential for application of nuclear matrix proteins in tumor diagnosis, assessment of tumor progression, and prognosis of therapies where properties of the transformed state of cells is modified. (umassmed.edu)
  • Nevertheless cells from many of these tissue must be attained through invasive techniques as well as the interindividual variability is certainly hard to regulate. (biomedigs.org)
  • Osteoblast cells exhibit increasing degrees of OA proteins throughout their differentiation. (biomedigs.org)
  • OA continues to be demonstrated seeing that needed for the working and differentiation of osteoblast cells [32]. (biomedigs.org)
  • A novel FAK Tyr397 inhibitor suppresses osteoblast proliferation and differentiation, as well as osteoclast formation, via PTHrP-induced RANKL expression in murine bone stromal ST2 cells [twenty]. (acthreceptor.com)
  • Igf 1 promotes bone formation by stimulating the differentiation and proliferation of osteoblasts, which are the cells responsible for bone formation. (jimcomunicaciones.com)
  • Igf 1 plays a crucial role in bone remodelling by stimulating the growth and development of bone cells, known as osteoblasts. (jimcomunicaciones.com)
  • Osteoblasts, polarized cuboidal cells specialized for the active secretion of ECM. (biomedcentral.com)
  • We isolated a new population of stem cells from human orofacial tissue gingiva, a tissue source easily accessible from the oral cavity, namely GMSC, which exhibited clonogenicity, self-renewal, and multipotent differentiation capacities. (ca.gov)
  • Osteoblast differentiation of rabbit adipose-derived stem cells by polyethylenimine-mediated BMP-2 gene transfection in vitro. (geneticsmr.com)
Human osteoblasts3
Mechanisms5
  • More recently, osteoblast cultures have been important in our understanding of drug mechanisms, tissue engineering and the osseointegration of bone with implants [ 2 ]. (biomedcentral.com)
  • However, the molecular mechanisms by which ER-α regulates osteoblast differentiation remain to be elucidated. (molcells.org)
  • Findings are presented which are consistent with involvement of nuclear structure in gene regulatory mechanisms which support osteoblast differentiation by addressing four principal questions: 1) Does the representation of nuclear matrix proteins reflect the developmental stage-specific requirements for modifications in transcription during osteoblast differentiation? (umassmed.edu)
  • IGF-I regulates the synthesis of bone matrix by two mechanisms: it increases the number of osteoblasts and controls the function of osteoblast differentiation 2 . (bvsalud.org)
  • However, the molecular mechanisms for APS in regulating SONFH remain unclear. (biomedcentral.com)
Induce3
  • Moreover, MSI2 regulated VSMC phenotypic switch through binding with Fbxo6 to induce Rnaset2 ubiquitination. (bvsalud.org)
  • Collectively, our results indicate that ER-α-induced Dlx3 activity may induce the osteoblast differentiation in a ligand independent manner. (molcells.org)
  • JNK can change the mRNA level of osteocalcin , so the activation of JNK can induce osteoblast differentiation. (cusabio.com)
Inhibit2
  • In the late stage of adipocyte differentiation, activated ERK1/2 phosphorylates PPARγ to inactivate it and inhibit adipocyte differentiation. (cusabio.com)
  • It is also regulated by hormones, such as growth hormone and estrogen, which can stimulate or inhibit its production. (jimcomunicaciones.com)
Vivo2
  • ANGPTL4 protein was observed by immunofluorescence in osteoclasts and osteoblasts in vivo. (ox.ac.uk)
  • We developed techniques to isolate mouse OMSCs derived from mandibles and verified their MSC characteristics by single colony formation, multi-lineage differentiation, and in vivo tissue regeneration. (ca.gov)
Vitro2
  • There is therefore, a need to consider whether the bone type that osteoblasts are isolated from could influence their in vitro characteristics. (biomedcentral.com)
  • If so, this may have implications for in vitro primary osteoblast culture studies, in addition to clinical considerations such as osseointegration of uncemented hip replacements. (biomedcentral.com)
Extracellular1
  • Currently, there are 4 known MAPK signaling pathways, including the extracellular signal-regulated kinase ( ERK ), c- Jun N-terminal kinase (JNK, also known as SAPK), p38 and ERK5 pathways. (cusabio.com)
Osteoclast2
  • Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4. (ox.ac.uk)
  • These results demonstrate that HIF is sufficient to enhance osteoclast-mediated bone resorption and that ANGPTL4 can compensate for HIF-1α deficiency with respect to stimulation of osteoclast activity and also augments osteoblast proliferation and differentiation. (ox.ac.uk)
Intracellular1
  • Taken together, our results, as decided using an miPSC-based platform, have exhibited that Cisd2 regulates mitochondrial function, proliferation, intracellular Ca2+ homeostasis, and Wnt pathway signaling. (healthyconnectionsinc.com)
Transcription factor1
  • Runt-related transcription factor 2 (RUNX2), a member of the RUNX family, functions as a critical regulator for osteoblast differentiation ( 14 ). (spandidos-publications.com)
Gene expression1
Trabecular3
  • There is no significant difference in mean doubling time, basal or mineralizing TNAP activity or mineralized area in osteoblasts derived from subchondral, cortical, or trabecular bone types from the canine femoral head. (biomedcentral.com)
  • Trabecular isolate osteoblasts have the least variation of the bone types studied, and therefore should be considered a preferred source for primary osteoblast cultures. (biomedcentral.com)
  • Within this research, we exactly recognized the place of constitutively expressed COX in standard bone, specifically in osteoblasts residing on the surface with the trabecular bone and in the periosteum and the endosteum of cortical bone within a mouse femur. (mirnasynthesis.com)
Adipocyte2
  • In the early stage of adipocyte differentiation, ERK1/2 promotes adipocyte differentiation by promoting the expression of C/EBPα and PPARγ. (cusabio.com)
  • The pathogenesis of SONFH is associated with increased intraosseous pressure caused by increased adipocyte proliferation and adipogenesis in the bone marrow, which can slow blood flow in the femoral head and eventually lead to ischemic osteonecrosis [ 4 ]. (biomedcentral.com)
Osteoclasts4
Deficiency1
  • In this system however, osteoblast differentiation of PSCs is selectively inhibited by Sp7 deficiency but PSCs are still present and may still play a role in skeletal growth. (nature.com)
Functions1
  • Studies in our laboratory have shown that some MSC functions are regulated by toll-like receptor (TLR) ligands. (weizmann.ac.il)
Osteoid2
  • Osteosarcoma is a malignant primary bone tumor that is characterized by neoplastic osteoblasts that produce osteoid. (medscape.com)
  • They are derived from terminally differentiated osteoblasts surrounded by unmineralized matrix (osteoid) during bone formation. (biomedcentral.com)
Selectively1
  • On the other hand, Mark et al showed that conditional deletion of Ptch selectively in experienced osteoblasts boosts hedgehog signaling and qualified prospects to enhanced osteoclastogenesis [9]. (acthreceptor.com)
Skeletal1
  • Dlx3 is essential for the skeletal development and plays an important role in osteoblast differentiation. (molcells.org)
Immune1
  • In addition to sleep promotion, melatonin also regulates hormone secretion, rhythms in reproductive activity, immune functionality, and circadian rhythms. (wikipedia.org)
Intramembranous1
  • Here we show that PSCs not only regulate intramembranous bone formation but also contribute significantly to endochondral bone formation. (nature.com)
Homeostasis2
  • Mitochondrial biogenesis and dynamic homeostasis are important for supplying a sufficient amount of energy for development and differentiation [8]. (healthyconnectionsinc.com)
  • The interactions between these factors are complex and tightly regulated to maintain bone homeostasis. (jimcomunicaciones.com)
Oxidation1
Function3
  • The work here provides baselines for canine osteoblast function, which has utility for future comparative studies. (biomedcentral.com)
  • The osteoblasts and osoteoclasts engage in a essential function in this approach. (acthreceptor.com)
  • The latter interact with the cytokines and growth factors, regulating their function [ 4 ]. (biomedcentral.com)