• Product Description google Mouse anti-Ectoderm-heural cortex protein 1 (ENC-1) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA. (biosensis.com)
  • What is a Recombinant Antibody and Why is it Important? (cellsignal.jp)
  • What is a Recombinant Antibody? (cellsignal.jp)
  • Mammalian cell lines are most commonly used for recombinant antibody production, although cell lines of bacterial, yeast, or insect origin are also suitable. (cellsignal.jp)
  • Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. (cellsignal.jp)
  • Unlike traditional methods for antibody production, recombinant approaches avoid the need to use animals. (cellsignal.jp)
  • Regardless of whether an antibody is polyclonal, monoclonal or recombinant, it must always be properly validated in the intended application prior to experimental use. (cellsignal.jp)
  • In this blog, we review an antibody-based method for protein knockdown called Trim-Away 1 that can help speed your research. (cellsignal.com)
  • The key component in this process is the intracellular E3 Ubiquitin ligase TRIM21, which binds to the Fc-region of the antibody and is ubiquitinated to the point that it is degraded by the proteasome, along with the bound antibody and its specific target. (cellsignal.com)
  • This induces ubiquitination and degradation of the antibody-target-TRIM21 complex in the proteasome. (cellsignal.com)
  • This Ring1B antibody was raised against the recombinant full-length mouse protein. (fishersci.com)
  • α-Synuclein is an abundant brain protein that binds to lipid membranes and is involved in the recycling of presynaptic vesicles. (ox.ac.uk)
  • MURF-1 also binds to ubiquitin-conjugating enzyme-9 and isopeptidase T-3, enzymes involved in small ubiquitin-related modifier-mediated nuclear import, and with glucocorticoid modulatory element binding protein-1 (GMEB-1), a transcriptional regulator. (rupress.org)
  • Moreover, PRIN2 and CSP41b form a distinct protein complex in vitro that binds DNA. (frontiersin.org)
  • The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. (cancerindex.org)
  • It is a substrate-specific adapter of an E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target protein. (biosensis.com)
  • Particularly in yeast, an organism where gluconeogenesis and glycolytic activity are intermittently coordinated, the multi-subunit GID E3 ligase complex specifically targets the surplus of gluconeogenic enzymes, including the conserved Fructose-1,6-bisphosphatase 1 (Fbp1), for proteasomal degradation. (elifesciences.org)
  • We therefore suggest that Xenopus laevis Rmnd5 , as a subunit of the CTLH complex, is a ubiquitin-ligase targeting an unknown factor for polyubiquitination and subsequent proteasomal degradation for proper fore- and midbrain development. (xenbase.org)
  • Ubiquitin itself can direct its targets to a number of different fates, including proteasomal degradation and membrane protein transport. (enzolifesciences.com)
  • E3 ubiquitin ligases regulate cellular protein composition by providing target recognition and specificity to the ubiquitin-dependent proteasomal degradation pathway 12 . (nature.com)
  • Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. (thermofisher.com)
  • Jumonji (JmjC) domain proteins (see Jarid2 ) influence gene expression and chromatin organization by way of histone demethylation, which provides a means to regulate the activity of genes across the genome. (sdbonline.org)
  • For example, NEDD8 activates SCF and related ubiquitin ligases, ISG15/UCRP is induced during in the antiviral interferon response, Apg12p and Apg8p regulate the autophagy pathway, and Hub1p modifies cell polarity factors. (enzolifesciences.com)
  • By binding to the adaptor protein SKP1 and serving as substrate receptors for the Skp1, Cullin, F-box E3 ubiquitin ligase complex, F-box proteins regulate critical cellular processes including cell cycle progression and membrane trafficking. (uga.edu)
  • miRNAs regulate proteins expressions by arresting or degrading the mRNAs. (cancerindex.org)
  • Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. (cusabio.com)
  • Cell division cycle protein 23 homolog is a protein in humans that is encoded by CDC23 gene. (cusabio.com)
  • We then investigated expression levels of genes involved in BMP signaling and regulation (for example, Smad1/5/8, TGFβ receptors type I and II, noggin, sclerostin, BMP and activin receptor membrane bound inhibitor (BAMBI), v-ski sarcoma viral oncogene homolog (Ski), Ski-related novel protein N (SnoN) and Smad ubiquitination regulatory factors (Smurfs)) and confirmed the expression of regulated genes at the protein level. (biomedcentral.com)
  • In vivo , the E3 ubiquitin ligase activity of the DCX complex is dependent on neddylation of the CUL4 subunit, though neddylation may be dispensable in some in vitro reactions. (bio-techne.com)
  • For this complex, neddylation is required for ligase activity in vivo , but generally not for in vitro activity. (bio-techne.com)
  • For in vitro applications, both complexes should work similarly and Catalog # E3-651 should be able to perform all of the functions of Catalog # E3-650. (bio-techne.com)
  • ATR kinase phosphorylated recombinant FANCI protein in vitro, which was facilitated by the presence of FANCD2. (elsevierpure.com)
  • Indeed, Hbp1 accumulates in cells lacking GID/CTLH activity, and Hbp1 physically interacts and is ubiquitinated in vitro by reconstituted GID/CTLH complexes. (elifesciences.org)
  • Recombinant antibodies are monoclonal, but their production involves in vitro genetic manipulation. (cellsignal.jp)
  • In the DCX complex (DDB1-CUL4-X-box), CUL4A serves as a scaffold that organizes the DDB1-X-box substrate recognition subunits with the RBX1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme. (bio-techne.com)
  • Structural basis for the subunit assembly of the anaphase-promoting complex. (ox.ac.uk)
  • Ubiquitin ligase Nedd4 promotes alpha-synuclein degradation by the endosomal-lysosomal pathway. (ox.ac.uk)
  • Here we show that the ubiquitin ligase Nedd4, which functions in the endosomal-lysosomal pathway, robustly ubiquitinates α-synuclein, unlike ligases previously implicated in its degradation. (ox.ac.uk)
  • This event also causes phosphorylation of the Fanconi anemia (FA) protein FANCI, triggering its monoubiquitination of the key DNA repair factor FANCD2 by the FA core E3 ligase complex, thereby promoting this central pathway of DNA repair which permits replication to be restarted. (elsevierpure.com)
  • The first two are mannose-6-phosphonate derivatives that serve as the inducers of protein degradation via the cellular endosomal-lysosomal degradation pathway. (nusl.cz)
  • The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. (antikoerper-online.de)
  • The Smad7 protein is negative regulator of the TGF-β signaling pathway, which is upregulated in patients with breast cancer. (cancerindex.org)
  • Bloom syndrome protein (BLM), a member of the RecQ family of helicases, is one such enzyme, with roles in HR, as well as telomere maintenance and replication [ 8 , 9 ]. (biomedcentral.com)
  • Covalent attachment, via an isopeptide bond, to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. (thermofisher.com)
  • E2 ubiquitin-conjugating enzymes play a central role in the ubiquitination cascade, shuttling ubiquitin from an E1 ubiquitin-activating enzyme to an E3 ligase-substrate complex. (amerigoscientific.com)
  • The E2 enzyme holds ubiquitin in its activated form via formation of a thioester bond between the carboxy terminus of ubiquitin and the E2 enzyme's active site cysteine. (amerigoscientific.com)
  • All E2 enzymes share a conserved "core" ubiquitin conjugating domain of about 150 amino acid residues, and many E2 enzymes possess additional N- and/or C-terminal protein sequences that can govern intracellular localization, confer regulatory properties, or provide specificity for interactions with particular E3 ligases. (amerigoscientific.com)
  • PfMSP3.1 is one of the surface proteins of the intracellular parasite Plasmodium falciparum, which causes malaria. (nusl.cz)
  • Recombinant antibodies offer several key advantages compared to traditional antibodies. (cellsignal.jp)
  • As such, recombinant antibodies are seeing increased use for scientific research, especially as a means of addressing the ongoing reproducibility crisis. (cellsignal.jp)
  • Recombinant antibodies are highly consistent from lot to lot, thereby ensuring reproducible experimental results. (cellsignal.jp)
  • Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.jp)
  • This protein degradation method can be applied to any endogenous protein without prior modification, uses antibodies that are widely available, and can be performed on a wide range of cell types. (cellsignal.com)
  • Using a cell's existing protein degradation machinery and antiviral reaction, the Trim-Away technique leverages target-specific antibodies to knock down unmodified native proteins within minutes. (cellsignal.com)
  • The antibodies that will have the best chance for success are immunoprecipitation-validated antibodies because they recognize the native structure of the proteins, which is the most important requirement for this method. (cellsignal.com)
  • Information on how its 13 constituent proteins are assembled, and how they interact with co-activators, substrates and regulatory proteins is limited. (ox.ac.uk)
  • Our structure explains how this TPR sub-complex, together with additional scaffolding subunits (Apc1, Apc4 and Apc5), coordinate the juxtaposition of the catalytic and substrate recognition module (Apc2, Apc11 and Apc10 (also known as Doc1)), and TPR-phosphorylation sites, relative to co-activator, regulatory proteins and substrates. (ox.ac.uk)
  • In the first part of our project, we identified new substrates for this ubiquitin ligase, the nuclear proteins ZXDA and ZXDB, with insufficiently characterized functions. (nusl.cz)
  • In yeast, the glucose-induced degradation-deficient (GID) E3 ligase selectively degrades superfluous gluconeogenic enzymes. (elifesciences.org)
  • This diploma thesis revolves around the preparation of biocompatible polymeric conjugates called "iBodies" that will be used to induce targeted lysosomal degradation of two model enzymes - Fibroblast activation protein α, and Glutamate carboxypeptidase II. (nusl.cz)
  • RNF2 (E3 ubiquitin-protein ligase RING2) is a ligase that mediates monoubiquitination of Lys-119 of histone H2A (H2AK119Ub). (fishersci.com)
  • This complex class is required to maintain the transcriptionally repressive state of many genes including Hox genes throughout development. (fishersci.com)
  • Over two dozen proteins involved in protein import and membrane insertion, peroxins, encoded by PEX genes, have been characterized. (tcdb.org)
  • This complex consists of an N-terminal 10-His tagged Cullin-4A, untagged RBX1, untagged DDB1 and untagged CRBN. (bio-techne.com)
  • Cullin-4A (CUL4A) is a core component of multiple cullin-RING type Ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes. (bio-techne.com)
  • Moreover, consistent with its known function as a component of the E3 in neddylation, the pattern of DCUN1D1 expression temporally correlates with an increase in the neddylated cullin fraction and stage-specific increases in the total ubiquitinated protein pool in wild-type mice. (bvsalud.org)
  • Furthermore, the effects of DCUN1D1 on spermatogenesis likely involve its regulation of cullin-RING-ligase (CRL)-type ubiquitin E3 activity during spermiogenesis through its role in promoting Cul3 neddylation. (bvsalud.org)
  • CUL3 is a conserved protein of the Cullin family, comprising eight members, which contain a conserved cullin homology domain, named after its ability to select cellular proteins for degradation. (nature.com)
  • The protein encoded by this gene is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. (novusbio.com)
  • COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases. (novusbio.com)
  • In this context, a new molecule, B-cell activating factor (BAFF), has emerged as a positive regulator of B cell survival and differentiation functioning through various signaling pathways and potentiating the activity of various receptor complexes through pleiotropic means. (medsci.org)
  • As one of the evasion strategies of the immunity system of the human host this protein interacts with one regulator of the complement system - C1 inhibitor. (nusl.cz)
  • CUL3 ASD-associated genetic variants are most often de novo missense or loss of function (loF) mutations, dispersed throughout the entire gene and affecting distinct protein domains. (nature.com)
  • The detection and repair of DNA damages is an important aspect of this process and is facilitated by proteins that comprise a number of distinct, while over-lapping, enzymatic repair pathways [ 1 ]. (biomedcentral.com)
  • Most peroxisomal membrane proteins do not have PTS1 or PTS2, and insertion requires several peroxins distinct from those required for import of soluble matrix proteins. (tcdb.org)
  • De novo loss of function mutations in the ubiquitin ligase-encoding gene Cullin3 ( CUL3) lead to autism spectrum disorder (ASD). (nature.com)
  • RNA viruses may escape acquired humoral and cellular immune responses by mutations in protective antigenic epitopes (e.g., avian influenza viruses), while accessory nonstructural proteins or multifunctional structural proteins interfere with the interferon system (e.g. (imperial.ac.uk)
  • As with many others, its biological function has not yet been elucidated in detail, although it is the only ubiquitin ligase whose mutations lead to the onset of a distal form of muscle atrophy. (nusl.cz)
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Foundational approaches for studying protein function in living models - gene editing and RNA interference (RNAi) - can cause unintended, non-specific defects, result in compromised phenotypes, or be difficult to implement in certain cell lines. (cellsignal.com)
  • Gene expression was determined by reverse transcription polymerase chain reaction (RT-PCR) and confirmed at the protein level by western blot. (biomedcentral.com)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • Here, we identified all subunits of the mammalian GID/CTLH complex and provide a comprehensive map of its hierarchical organization and step-wise assembly. (elifesciences.org)
  • In this review we provide current evidence for BAFF-dependent signaling through the NgR multimeric complex, elucidating their association within the CNS compartment and underlying the importance of these potential pathogenic molecular regulators as possible therapeutic targets to limit relapse rates and potentially MS progression. (medsci.org)
  • Biochemical reconstitution demonstrates that the mammalian complex possesses inherent E3 ubiquitin ligase activity, using Ube2H as its cognate E2. (elifesciences.org)
  • Our new publication in Journal of Biological Chemistry: Role of the EF-hand-like Motif in the 14-3-3 Protein-mediated Activation of Yeast Neutral Trehalase Nth1. (cas.cz)
  • While F-box proteins are conserved throughout eukaryotes and are well studied in yeast, plants, and animals, studies in parasitic protozoa are lagging. (uga.edu)
  • The anaphase-promoting complex or cyclosome (APC/C) is an unusually large E3 ubiquitin ligase responsible for regulating defined cell cycle transitions. (ox.ac.uk)
  • The complex is also involved in phosphorylation of p53/TP53, JUN, I-kappa-B-alpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. (antikoerper-online.de)
  • Despite the well-understood process of CUL3-mediated protein ubiquitination and degradation 12 , its target proteins in the developing central nervous system and its role in brain development remain utterly understudied. (nature.com)
  • The Human Papillomavirus (HPV) E2 protein, which inhibits the E6 and E7 viral oncogenes, is believed to have antioncogenic properties. (docksci.com)
  • Transforming growth factor β1 inhibits bone morphogenic protein (BMP)-2 and BMP-7 signaling via upregulation of Ski-related novel protein N (SnoN): possible mechanism for the failure of BMP therapy? (biomedcentral.com)
  • Overexpression of HSPA12A in RCC cells unstabilizes CD147 through increasing its ubiquitin-proteasome degradation, thereby inhibits lactate export and glycolysis, and ultimately suppresses RCC cell migration. (thno.org)
  • Purified Nedd4 recognizes the carboxyl terminus of α-synuclein (residues 120-133) and attaches K63-linked ubiquitin chains. (ox.ac.uk)
  • This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. (thermofisher.com)
  • A marked increase in IFN-induced DR4 protein expression after tocilizumab treatment was observed by immunohistochemical staining in the tumor from the mice xenograft model. (oncotarget.com)
  • Among methods we use are recombinant protein expression, biophysical characterization, study of intermolecular interactions, protein structure and interaction surfaces. (cas.cz)
  • Levels of neddylated Cul3 were decreased in DCUN1D1-/- mice, and ubiquitinated proteins did not accumulate during the stages in which DCUN1D1 expression peaks during spermatogenesis in wild-type mice. (bvsalud.org)
  • You can either use a specific expression database such as The Human Protein Atlas and BioGPS or you can perform a quick qPCR/western blot (WB) experiment. (cellsignal.com)
  • The experimental part includes the cloning of plasmids to produce C1 inhibitor and various forms of merozoite surface protein PfMSP3.1, transfection of S2 insect cells with these plasmids, subsequent protein expression in S2 cells and their purification. (nusl.cz)
  • All these methods enable us to better understand the details how the activity and function of protein-protein complexes is regulated. (cas.cz)
  • Post-translational modification by ubiquitin and ubiquitin-like proteins (Ubls) is an essential cellular regulatory mechanism, allowing rapid and reversible control of a target protein's function by altering its half-life, sub-cellular localization, enzymatic activity, protein-protein interactions, or other properties. (enzolifesciences.com)
  • Taken together, our results suggest that PEP activity and consequently the switch from NEP to PEP activity, is essential during embryo development and that the PRIN2-CSP41b DNA binding protein complex possibly is important for full PEP activity during this process. (frontiersin.org)
  • The biological activity of recombinant human (rh)BMPs (2, 4 and 7) has been defined by using a variety of animal models. (biomedcentral.com)
  • An extract of the coralline demosponge Astrosclera willeyana inhibited the ubiquitin ligase activity of the immunomodulatory protein Cbl-b. (cancer.gov)
  • Its central postulate is based on the fact that instead of suppressing protein activity with low-molecular inhibitors, we can instead use molecular tools to hijack the host organism's own degradation pathways and force it to degrade chosen proteins by itself. (nusl.cz)
  • The ligase activity is enhanced by BMI1/PCGF4. (fishersci.com)
  • Q: What is the main difference between the Recombinant Human CUL4A/NEDD8/RBX1/DDB1/CRBN Protein, CF (Catalog # E3-651) and the Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF (Catalog # E3-650)? (bio-techne.com)
  • In human cells, Nedd4 overexpression enhances α-synuclein ubiquitination and clearance by a lysosomal process requiring components of the endosomal-sorting complex required for transport. (ox.ac.uk)
  • JmjC proteins have been associated with many human diseases including various cancers, developmental and neurological disorders, however, the shared biology and possible common contribution to organismal development and tissue homeostasis of all JmjC proteins remains unclear. (sdbonline.org)
  • Immunogen Description Partial recombinant human ENC-1 (amino acids 17-98) with a GST tag. (biosensis.com)
  • Xenopus laevis Rmnd5 protein is structurally and functionally related to human RMND5A . (xenbase.org)
  • Our results show the the structural glimpse on the 14-3-3-dependent regulation of human ubiquitin ligase Nedd4-2. (cas.cz)
  • Stranglehold on the spindle assembly checkpoint: the human papillomavirus E2 protein provokes BUBR1-dependent aneuploidy. (docksci.com)
  • COPS8 (AAH03090, 1 a.a. - 209 a.a.) full-length human protein. (novusbio.com)
  • Consequently, it was expected that topical application of recombinant human (rh)BMP-2 and rhBMP-7 would improve the healing of complex fractures. (biomedcentral.com)
  • The N-terminal End of hNICD1 Contains a Degron Required for Degradation in Extract Following we evaluated the proceeds rates of NICD healthy proteins in remove versus cultured human cellular material. (healthyconnectionsinc.com)
  • A: The main difference between these products is that Catalog # E3-651 includes NEDD8 in the complex with CUL4A, RBX1, DDB1, and CRBN. (bio-techne.com)
  • Catalog # E3-650 contains all of the same proteins in a complex (except NEDD8), but CUL4A is not neddylated. (bio-techne.com)
  • To investigate the functional significance of this interaction, we expressed green fluorescent protein fusion constructs encoding defined fragments of titin's M-line region and MURF-1 in cardiac myocytes. (rupress.org)
  • New knowledge about the structure of the complex may enable the development of specific inhibitors of the interaction between these two proteins, and subsequently in the development of new drugs aimed at the selective elimination of senescent cells. (cas.cz)
  • During interphase, RanGAP1 is bound to the cytoplasmic side of the nuclear pore complex via a sumoylationdependent interaction with the IR domain of the large nucleoporin RanBP2/Nup358. (enzolifesciences.com)
  • This phenotype is associated with interaction of E2 with the Mitotic Checkpoint Complex (MCC) proteins Cdc20, MAD2 and BUBR1. (docksci.com)
  • In this study, we demonstrate that hSSB1 and BLM helicase form a complex in cells and the interaction is altered in response to ionising radiation (IR). (biomedcentral.com)
  • An interaction of HSPA12A with HRD1 ubiquitin E3 ligase was detected in RCC cells. (thno.org)
  • We have identified eighteen putative F-box proteins in the Toxoplasma genome of which four have predicted homologs in Plasmodium . (uga.edu)
  • Our Laboratory is focused on structural biology (the relationship between the structure and function of certain groups of proteins), particularly we focus on the proteins which participate in the signal transmission in the cell. (cas.cz)
  • Our new paper in JBC: Structural Insight into the 14-3-3 Protein-Dependent Inhibition of Protein Kinase ASK1. (cas.cz)
  • The specific CRLs required for spermiogenesis and their protein targets require identification. (bvsalud.org)
  • With this in mind, long-lived proteins that were previously not targetable are now prime targets for knockdown experiments. (cellsignal.com)
  • This sheds light upon the role of the Smad7 protein in this system. (lu.se)
  • In interphase parasites, TgFBXO1 is a component of the Inner Membrane Complex (IMC), which is an organelle that underlies the plasma membrane. (uga.edu)
  • 2011) addresses two of these aspects, the translocation of soluble proteins into the peroxisomal matrix and the biogenesis of the peroxisomal membrane. (tcdb.org)
  • 2011 ) have reviewed the processes by which matrix and membrane proteins are incorporated into the peroxysome. (tcdb.org)
  • The membrane bound docking subcomplex includes several Pex proteins, Pex13p, 14p and 17p. (tcdb.org)
  • Pex4p is a membrane protein that may facilitate cycling of Pex5p back to the cytosol. (tcdb.org)
  • The COOH-terminal A168-170 region of the giant sarcomeric protein titin interacts with muscle-specific RING finger-1 (MURF-1). (rupress.org)
  • Under stress conditions, p53 interacts with another transcription factor, FOXO4 (Forkhead box O 4), and together they increase the production of p21 protein, which triggers the process of cell aging (senescence). (cas.cz)
  • TgFBXO1 interacts with Toxoplasma SKP1 confirming it as a bona fide F-box protein. (uga.edu)
  • Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. (cancerindex.org)
  • Polycomb Repressive Complex 2 (PRC2), a component of the Polycomb machinery, is responsible for the methylation of histone H3 lysine 27 (H3K27me2/3). (sdbonline.org)
  • It has been demonstrated that the target lysine of RanGAP1, as well as the C-terminus of mature SUMO-1, lie within mobile regions of the two proteins. (enzolifesciences.com)
  • SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. (thermofisher.com)
  • This project focused on the molecular and functional characterization of the SCFFBXO38 ubiquitin ligase. (nusl.cz)
  • Polycomb Group (PcG) proteins maintain transcriptional repression throughout development, mostly by regulating chromatin structure. (sdbonline.org)
  • Using genetic and biochemical methods, we have shown that ZXDA/B proteins act as positive regulators of centromeric chromatin integrity and that experimental inactivation of the SCFFBXO38 ubiquitin ligase resulted in a ZXDA/B-dependent stabilization of CENP-A and CENP-B proteins in the centromeric regions. (nusl.cz)
  • The PcG PRC1 complex acts via chromatin remodeling and modification of histones and rendering chromatin heritably changed in its expressibility. (fishersci.com)
  • Lactate export, glycolysis rate, and CD147 protein abundance were also inhibited by HSPA12A overexpression but promoted by HSPA12A knockdown. (thno.org)
  • In a work published in the Protein Science journal, scientific teams of Dr. Obsilova (IPHYS CAS), prof. Obsil (Faculty of Science, Charles University and IPHYS CAS) and their colleagues from IOCB CAS characterized interactions between p53 and FOXO4 at the molecular level. (cas.cz)
  • Mass spectrometry, immunoprecipitation, and immunoblotting were employed to examine protein-protein interactions. (thno.org)
  • The Trim-Away technique offers several key advantages over existing methods of protein knockdown, making it an optimal research tool that can be established in the lab with little time investment. (cellsignal.com)
  • Faster, easier protein knockdown is possible without the need to change your laboratory setup or invest in new training or equipment. (cellsignal.com)
  • Here we show that Rmnd5 , the Gid2 orthologue from Xenopus laevis, is an ubiquitin-ligase embedded in a high molecular weight complex. (xenbase.org)
  • MSY3 is a DNA binding protein, which acts as a transcriptional repressor. (biomedcentral.com)
  • In the existing model transcriptional termination Hs.76067 is definitely mediated simply by the E3 ubiquitin ligase complex SCFFbxw7 which helps bring about ubiquitin-mediated destruction of NICD in a INFESTATION domain-dependent method (Moretti and Brou 2013 Herein all of us identify a hNICD1-specific degron within the N-terminal region specific from its C-terminal PEST area. (healthyconnectionsinc.com)
  • In vertebrates a requisite for the formation of this gradient is the localization of sumoylated RanGAP1 in complex with RanBP2 at the mitotic spindle and with the kinetochores. (enzolifesciences.com)
  • SUMO1 is involved, for instance, in targeting RANGAP1 to the nuclear pore complex protein RANBP2. (thermofisher.com)
  • The Y-box protein MSY3/Csda represses myogenin transcription in skeletal muscle by binding a highly conserved cis-acting DNA element located just upstream of the myogenin minimal promoter (myogHCE). (biomedcentral.com)
  • MSY3 (MSY4, YB-2, CSDA, dbpA, ZONAB) is a member of the cold shock domain (CSD) family of proteins also known as Y-box proteins, which are evolutionarily conserved proteins that function as transcription factors and regulators of RNA metabolism and protein synthesis. (biomedcentral.com)
  • Liberated NICD enters the nucleus wherever it forms a complex with CSL (CBF1/RBPjk/Su(H)/Lag-1) MAML (Mastermind-like) and CoA (coactivators) (Kovall and Blacklow 2010 Development of this complicated drives transcription of Level target genetics. (healthyconnectionsinc.com)
  • MybiosourceProduct Short Name: [G-Protein Coupled Receptor 126 (GPR126)]Product Name Synonyme: [G-Protein Coupled Receptor. (biocheminfo.org)