Recombinant proteins pichia. Medical search. Frequent questions

Pichia pastoris , a methylotrophic yeast, is our favourite host, which we employ for expression and secretion of recombinant soluble proteins as well as for the biosynthesis of valuable metabolites, e.g. plant terpenoids, through heterologous expression of membrane proteins. (tugraz.at)
We have identified a novel isoform of the pig liver esterase protein family, markedly improved expression of pig liver esterases in P. pastoris and E. coli, and have evolutionarily engineered pig liver esterases for biocatalytic applications. (tugraz.at)
We have devised a novel, medium-throughput, random mutagenesis/screening protocol for improved protein secretion in P. pastoris. (tugraz.at)
We have applied multiple approaches to identify key genes that determine protein secretion rates in P. pastoris. (tugraz.at)
Enhanced Production of Recombinant Secretory Proteins in Pichia pastoris by Optimizing Kex2 P1' site. (stembook.org)
DESCRIPTION (provided by applicant): With its ability to secrete high levels of recombinant proteins, the yeast Pichia pastoris is extensively used for protein manufacturing. (sbir.gov)
However, as actual yields vary widely for individual proteins, GlycoFi is focusing on the improvement of protein yields in P. pastoris as part of the company's long term mission to develop yeast-based protein production technology for manufacturing therapeutic proteins with human glycosylation structures. (sbir.gov)
To tackle this issue, we evaluated transcriptomics datasets of the yeast Pichia pastoris (syn. (boku.ac.at)
Together with the observed impact on the transcriptome and total protein content, our results indicate that the capacity of P. pastoris for protein production is not at its limit yet. (boku.ac.at)
Her recent research efforts developed out of a collaboration with Dr. William A. Dunn and focus on the role of the Vac8 protein in a cellular recycling process known as autophagy using the yeast Pichia pastoris as the model system. (bradley.edu)
The second involves engineering the expression of recombinant GFP-tagged human keratin proteins in Pichia pastoris to provide a genetically modifiable source of the biopolymer for use in the preparation of functional composites carried out by Dr. Haverhals' research group. (bradley.edu)
This method for the first time describes reliable experimental procedures for efficient inducible methanol-free recombinant protein production in Pichia pastoris (Komagataella phaffii) , employing the carbon source regulated promoters P DC and P DF in small scale experiments while cultivation parameters can be monitored online, and a constant glycerol feed is applied. (jove.com)
This method can help in the pre selection of chromosomes clones by Pichia pastoris strains expressing recombinant proteins under de-repressed conditions by a simple shake flask cultivations prior to up scaling. (jove.com)
The main advantage of this technique is that high level methanol free protein expression can be achieved using Pichia pastoris, which is supported by the exact online monitoring of important cultivation parameters. (jove.com)
Pichia pastoris (Komagataella phaffii) is widely used for industrial production of heterologous proteins due to high secretory capabilities but selection of highly productive engineered strains remains a limiting. (biomedcentral.com)
rFg14-3-3e protein was expressed in Pichia pastoris . (biomedcentral.com)
The production of recombinant proteins by Pichia pastoris under AOX1 promoter is usually performed using methanol together with either glycerol or sorbitol as co-substrate. (pucv.cl)
The yeast Pichia pastoris expression system was investigated for the production of human cathepsin C (CatC) recombinant protein. (iyte.edu.tr)
QP10666 protein expressed in Pichia Pastoris. (enquirebio.com)
Results: A scalable bioprocess for fine-tuned co-expression of hCYP2C9 and its essential complementary human cytochrome P450 reductase (hCPR) in the yeast Pichia pastoris (Komagataella phaffii) is presented. (uab.cat)
Escherichia coli and Pichia pastoris (single ou multicopy clone strategy). (xpress-biologics.com)
Further, a yeast called Pichia pastoris, expressed RBD-based vaccine against severe acute respiratory syndrome coronavirus (SARS-CoV) in mice. (news-medical.net)
Our goal was to develop a general and convenient strategy to improve heterologous protein synthesis in Pichia pastoris without mutating the expressed genes. (biomedcentral.com)
Here, a large-scale deletion library of ribosomal protein (RP) genes was constructed for heterologous protein expression in Pichia pastoris , and 59% (16/27) RP deletants have significantly increased heterologous protein yield. (biomedcentral.com)
Pichia pastoris is a widely used platform for heterologous protein expression, which is a "generally regarded as safe" (GRAS) microorganism. (biomedcentral.com)
Unlike the bacterial expression systems, which lack the modification enzymes, Pichia pastoris is able to produce heterologous proteins with post-translational modifications, especially glycosylation, which is crucial for optimal properties of many pharmaceutical proteins. (biomedcentral.com)
Over 5000 proteins were manufactured in P. pastoris (data from RCT Pichia technology), most of which are industrial enzymes and biopharmaceutical proteins [ 1 , 2 ]. (biomedcentral.com)
However, due to the remarkable codon preference and tRNA content against the other species, heterologous proteins expressed in Pichia pastoris often encounter folding problem, which severely limited their production efficiency [ 3 ]. (biomedcentral.com)
Recombinant protein produced in Pichia pastoris. (cellsystems.eu)
This database hosts up-to-date annotation information of the genome of the methylotrophic yeast Pichia pastoris ( Komagataella spp. (boku.ac.at)
Pichia pastoris was first isolated in 1920 from the exudate of a chestnut tree in France, and described by A. Guilliermond as Zygosaccharomyces pastori [1]. (boku.ac.at)
Further strains were isolated from trees in California by H. Phaff, who named the species Pichia pastoris in 1956 [2]. (boku.ac.at)
The established Pichia pastoris protein production platforms are based on strains of either K. phaffii or K. pastoris [7]. (boku.ac.at)
To avoid confusion and to include all strains employed in biotechnology we use the established name Pichia pastoris here as a synonym for all Komagataella species, giving reference to the different species for different strains, as listed in the table below. (boku.ac.at)
1985. Pichia pastoris as a host system for transformations. (boku.ac.at)
nov., a new sibling species of Komagataella (Pichia) pastoris based on multigene sequence analysis. (boku.ac.at)
2009. Biotechnological strains of Komagataella (Pichia) pastoris are Komagataella phaffii as determined from multigene sequence analysis. (boku.ac.at)
2009. Genome sequence of the recombinant protein production host Pichia pastoris. (boku.ac.at)
2009. Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris. (boku.ac.at)
2011. High-quality genome sequence of Pichia pastoris CBS7435. (boku.ac.at)
The effect of α-mating factor secretion signal mutations on recombinant protein expression in Pichia pastoris. (pacific.edu)
The methylotrophic yeast, Pichia pastoris , has been genetically engineered to produce many heterologous proteins for industrial and research purposes. (pacific.edu)
The methylotrophic yeast species Komagataella phaffii (synonym: Pichia pastoris) is widely used as a host for recombinant protein production. (ipaqt.org)
Therefore, the industry turned to alternatives such as Chinese hamster ovary cells and Pichia pastoris , which have significant limitations for multi-parameter optimisation. (phenotypeca.com)
ASAG22 was codon-optimized and co-expressed with five different molecular chaperones (PDI, CPR5, ERO1, HAC1, and Bip) in order to improve the expression level of FumDM in Pichia pastoris (also known as Komagataella phaffii) GS115. (inra.fr)
The heterologous P protein expressed by Pichia pastoris self-assembles into P particles. (redwoodbioscience.com)
Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. (lu.se)

Accumulation of aminoriboflavin and roseoflavin by K. phaffii recombinant strain in a bioreactor reached 22 and 130 mg L − 1 , respectively. (biomedcentral.com)

nov. and the transfer of Pichia pseudopastoris to the methylotrophic yeast genus Komagataella. (boku.ac.at)

E. coli strain BL21 and BL21(DE3) are two strains commonly used for protein production. (wikipedia.org)
Bon Opus has established an efficient system for yeast cell recombinant protein expression, which includes the design of signal sequences, expression vectors, and a set of yeast strains (X33, GS115, KM71, and SMD1168). (bonopusbio.com)
We offer a custom specific high-level recombinant protein coli expression service using a variety of commercially available strains. (protein-technologies.com)
For comparison, recombinant strains of the native bacterial producer S. davaonensis accumulated near one-order less of roseoflavin while no recombinant producers of aminoriboflavin was reported at all. (biomedcentral.com)
QTL technology combines Phenotypeca's proprietary breeding method with genomic based screening to optimise strains for a given protein and the requirements of its market. (phenotypeca.com)
The enzyme activities of recombinant strains over-expressing PDI and CPR5 reached the highest levels of 259.47 U/mL and 161.34 U/mL, 635% and 357% higher than the original enzyme activity, respectively. (inra.fr)
Transcriptomic analysis of the two recombinant strains in comparison with the control strain showed that the correct folding of proteins assisted by molecular chaperones played a key role in the improvement of FumDM expression and its enzyme activity. (inra.fr)

Baculovirus expression is a powerful and versatile system for producing high levels of recombinant protein expression in insect cells. (protein-technologies.com)

This includes the transcription of the recombinant DNA to messenger RNA (mRNA), the translation of mRNA into polypeptide chains, which are ultimately folded into functional proteins and may be targeted to specific subcellular or extracellular locations. (wikipedia.org)
The Sm -TSP-2 schistosomiasis vaccine is comprised of a 9 kDa recombinant protein corresponding to the extracellular domain of a unique S. mansoni tetraspanin. (bcm.edu)
Extracellular recombinant proCatC was further activated by cysteine endoprotease papain in vitro and its activity was confirmed by assays using a synthetic substrate. (iyte.edu.tr)
In order to secrete proteins for easier purification from the extracellular medium, the coding sequence of recombinant proteins is initially fused to the Saccharomyces cerevisiae α-mating factor secretion signal leader. (pacific.edu)

Most recombinant proteins are currently produced in prokaryotic cells (mainly the bacterium Escherichia coli ) and a small number of well-characterized mammalian cell lines, such as Chinese hamster ovary (CHO) cells. (frontiersin.org)

For example, a DNA sequence for a protein of interest could be cloned or subcloned into a high copy-number plasmid containing the lac (often LacUV5) promoter, which is then transformed into the bacterium E. coli. (wikipedia.org)
Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)
For example, green fluorescent protein (GFP) is a eukaryotic protein containing 11 beta-sheet structures and its folding yield was significantly increased by co-translational folding in E. coli [ 14 ]. (biomedcentral.com)
Another protein, phytase (Phy) from Citrobacter amalonaticus CGMCC 1696, has 95% homology to E. coli -derived phytase which consists of one α-domain containing five α-helices and a β-hairpin, and one α/β-domain including seven β-sheets and four α-helices [ 15 ]. (biomedcentral.com)

Therapeutic proteins (e.g., antibodies, vaccines, enzymes, cytokines, and growth factors) account for almost half of this market, followed by industrial proteins (e.g., technical enzymes) and research reagents (e.g., antibodies for protein detection and purification) ( Markets and Markets, 2017 ). (frontiersin.org)
Market growth has been supported by advances in recombinant protein production technologies, including the engineering of expression hosts, the optimization of upstream cultivation (e.g., bioreactor design, nutritional, and physical parameters), and the development of more efficient protein extraction and purification methods. (frontiersin.org)
We will take the fully optimized protein and scale-up with purification as it suits the protein of interest. (bonopusbio.com)
This yeast has the ability to produce and secrete large quantities of protein in the cultivation medium, which facilitates the recovery of the biological material and significantly decreases the contamination with host cell proteins and facilitates the purification. (xpress-biologics.com)
As well proving a range of expression and purification facilities, PTL can offer a GLP-compliant bioprocess services to produce recombinant proteins. (protein-technologies.com)
Institution of a two-step purification scheme for tag-free recombinant Taiwan native norovirus P and VP1 proteins The protruding (P) area of the necessary factor capsid protein VP1 of norovirus (NoV) is the necessary concern for immune recognition and host receptor binding. (redwoodbioscience.com)
Purification and characterization of two protein kinases acting on the aquaporin SoPIP2;1. (lu.se)

Proteins are also widely used in industry, including enzymes used to manufacture textiles and chemicals and to process food and feed. (frontiersin.org)
abstract = "Background: Currently, the numerous and versatile applications in pharmaceutical and chemical industry make the recombinant production of cytochrome P450 enzymes (CYPs) of great biotechnological interest. (uab.cat)
A potential expression platform for efficient pharmaceutical proteins and industrial enzymes production was provided without synonymous mutation. (biomedcentral.com)
Using tobacco plant expression the secretion, post-translational modifications and high expression of recombinant proteins ranging from recombinant enzymes to therapeutic targets and vaccines can be achieved using this system. (protein-technologies.com)
The HMW agents that were considered most relevant were flour dusts, enzymes (both plant and animal derived), gums, foods and tobacco, rubber-derived proteins, animal- and insect-derived allergens, and fish/ seafood-derived allergens ( Table 1 ). (cdc.gov)
Enzymes are proteins used as biocatalysts to reduce or replace the use of chemi- cals in a variety of processes.9 As catalysts, enzymes are used in a variety of indus- tries, including cleaning, food processing, animal feed, fuel alcohol, textile, paper, and pharmaceuticals. (cdc.gov)

Sm -TSP-2 was expressed as a recombinant protein secreted by the yeast Pichia Pink , and purified in a two-step process, which resulted in a protein recovery yield of 31 percent and a protein purity of 97 percent The developed processes are suitable for the production of purified protein for subsequent formulation and Phase 1 clinical studies. (bcm.edu)

Our particular focus in the yeast lipid field are sterols, their biosynthesis and homeostasis, and their interactions with membrane proteins. (tugraz.at)
The translational pausing sites, which are mediated by clustered but non-consecutive slow-translating codons, coordinate protein biosynthesis and co-translational folding [ 6 ]. (biomedcentral.com)

Protein production is the biotechnological process of generating a specific protein. (wikipedia.org)
Protein production systems (also known as expression systems) are used in the life sciences, biotechnology, and medicine. (wikipedia.org)
Commonly used protein production systems include those derived from bacteria, yeast, baculovirus/insect, mammalian cells, and more recently filamentous fungi such as Myceliophthora thermophila. (wikipedia.org)
To combine the high yield/productivity and scalable protein features of bacteria and yeast, and advanced epigenetic features of plants, insects and mammalians systems, other protein production systems are developed using unicellular eukaryotes (i.e. non-pathogenic 'Leishmania' cells). (wikipedia.org)
Over the last three decades, the expression of recombinant proteins in plants and plant cells has been promoted as an alternative cost-effective production platform. (frontiersin.org)
Although plant systems are now gaining widespread acceptance as a platform for the larger-scale production of recombinant proteins, there is still resistance to commercial uptake. (frontiersin.org)
Nevertheless, some plant-derived proteins for research or cosmetic/pharmaceutical applications have reached the market, showing that plants can excel as a competitive production platform in some niche areas. (frontiersin.org)
Here, we discuss the strengths of plant expression systems for specific applications, but mainly address the bottlenecks that must be overcome before plants can compete with conventional systems, enabling the future commercial utilization of plants for the production of valuable proteins. (frontiersin.org)
There are also several platforms based on plants and plant cells, but these have not been included in the latest market studies, indicating they have not yet commanded a significant share of commercial protein production capacity. (frontiersin.org)
Yeasts are widely used cell factories for commercial heterologous protein production, however, specific productivities are usually tightly coupled to biomass formation. (boku.ac.at)
Our yeast protein production workflow is distinct, with a hands-on approach for each application. (bonopusbio.com)
To begin our custom recombinant protein production in yeast cells, we spend two weeks synthesizing the cells, undergoing codon optimization, and preparing the DNA. (bonopusbio.com)
At the end of all yeast protein production services, we complete our in-house quality control and prepare the yields for on-time delivery. (bonopusbio.com)
Expression systems for lactic acid bacteria have been developed for metabolic engineering applications as well as for food-grade recombinant protein production. (biomedcentral.com)
Various assays were used to investigate the stimulatory effects of the purified rFg14-3-3e protein on specific functions of goat PBMCs, including cytokine secretion, proliferation, migration, nitric oxide (NO) production, phagocytosis, and apoptotic capabilities. (biomedcentral.com)
We have developed a recombinant protein production platform and have produced more than 30 different proteins for therapeutic and imaging applications. (xpress-biologics.com)
Expression optimization, flask and bioreactor production, rapid scale up to 200 L/batch, growth monitoring, protein refolding and tag removal as requested and full cGMP production. (protein-technologies.com)
This strain was then used as a basis for a development of the protein production system [3]. (boku.ac.at)

The best expression system depends on the gene involved, for example the Saccharomyces cerevisiae is often preferred for proteins that require significant posttranslational modification. (wikipedia.org)

Proposed Commercial Application: GlycoFi's Humanized Yeast(tm) enables the study of glycosylation structure-function relationships and creates the ability not only to pinpoint the specific glycoforms that optimize the bioactivity, pharmacokinetics and overall efficacy of a therapeutic protein, but also to manufacture them at low cost with a high degree of uniformity. (sbir.gov)
Because of its involvement in F. gigantica recognition by innate immune cells, rFg14-3-3e protein may have applications for development of diagnostics and therapeutic interventions. (biomedcentral.com)

Hence, multi-domain eukaryotic proteins expressed in bacteria often are non-functional. (wikipedia.org)
To address these concerns, expressions systems using multiple eukaryotic cells were developed for applications requiring the proteins be conformed as in, or closer to eukaryotic organisms: cells of plants (i.e. tobacco), of insects or mammalians (i.e. bovines) are transfected with genes and cultured in suspension and even as tissues or whole organisms, to produce fully folded proteins. (wikipedia.org)
The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)
The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
Yeast is an excellent host cell strain for recombinant eukaryotic expression demands because it's fairly easy to manipulate during the workflow. (bonopusbio.com)

Microenvironmental control of hematopoietic stem cell fate via CXCL8 and protein kinase C. Cell Rep. 2023;42(5):112528. (stembook.org)

We engineer the membranes of microbes, i.e. bacteria and yeasts, regarding lipid and membrane protein composition, either to understand the complex interactions of these hydrophobic compounds or to apply the modified microorganisms for interesting goals of industrial biotechnology. (tugraz.at)

Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antikoerper-online.de)
Western blot and immunofluorescence assay (IFA) were used to examine the reactivity of rFg14-3-3e protein to anti- F. gigantica and anti-rFg14-3-3e antibodies, respectively. (biomedcentral.com)
Western blot analysis showed that rFg14-3-3e protein is recognized by sera from goats experimentally infected with F. gigantica and immunofluorescence staining using rat anti-rFg14-3-3e antibodies demonstrated the specific binding of rFg14-3-3e protein to the surface of goat PBMCs. (biomedcentral.com)

We offer over 30 years of combined experience in the manufacturing of recombinant proteins following all expression solutions, i.e. periplasmic expression, soluble cytoplasmic expression, secretion and inclusion bodies refolding. (xpress-biologics.com)
Decelerating translation elongation speed has been shown to improve the soluble protein yield when expressing heterologous proteins in industrial expression hosts. (biomedcentral.com)
Water-soluble proteins of royal jelly were fractionated and investigated for the proliferative and migratory effects on human epidermal keratinocytes (HaCaT) in an in vitro wound healing model. (biomedcentral.com)
We fractionated the water-soluble proteins of royal jelly and identified one fraction (Fraction 2) that induced both proliferative and migratory effects on a human epidermal keratinocyte cell line. (biomedcentral.com)

Though some mutations clearly dampened protein expression, deletion of amino acids 57-70, corresponding to the predicted 3rd alpha helix of α-mating factor secretion signal, increased secretion of reporter proteins horseradish peroxidase and lipase at least 50% in small-scale cultures. (pacific.edu)

Recombinant KRAS G12D Protein Vaccines Elicit Important Anti-Tumor Ends in Mouse CT26 Tumor Fashions Drug progress concentrating on almost definitely in all probability essentially the most continuously mutation G12D of KRAS has good significance. (redwoodbioscience.com)

The demand for recombinant proteins is therefore rising steadily, with a market valued at US$1.654 billion in 2017 predicted to reach US$2.850 billion by 2022 ( Markets and Markets, 2017 ). (frontiersin.org)

It is typically achieved by the manipulation of gene expression in an organism such that it expresses large amounts of a recombinant gene. (wikipedia.org)
Nonetheless, bacterial expression has the advantage of easily producing large amounts of protein, which is required for X-ray crystallography or nuclear magnetic resonance experiments for structure determination. (wikipedia.org)
Even so, plants as an alternative expression platform offer unique advantages, particularly when target proteins are difficult to produce in conventional systems, require specific qualitative properties such as particular glycan profiles, and/or must be produced on a larger scale in response to urgent demand. (frontiersin.org)
We have uncovered several endogenous yeast proteins and mechanisms that render protein secretion or membrane protein expression more efficient and sustainable. (tugraz.at)
Bon Opus Biosciences is an expert in recombinant protein expression with yeast host cells. (bonopusbio.com)
We guarantee our yeast protein expression services function similarly to other host cells, so you can rest assured knowing your selection is accurate and handled properly. (bonopusbio.com)
We report on the cloning and expression of a recombinant F. gigantica 14-3-3 epsilon protein (rFg14-3-3e), and testing its effects on specific functions of goat peripheral blood mononuclear cells (PBMCs). (biomedcentral.com)
The expressed protein was secreted into the culture medium through the α-factor mating signal sequence of the expression vector. (iyte.edu.tr)
We screened for early-stage osteogenesis- or adipogenesis-based MSCs protein expression profiles using TMT-based quantitative proteomic analysis to identify novel participating molecules. (bvsalud.org)
The advantages of insect cell expression are that it offers posttranslational modifications similar to mammalian cells with high levels of protein expression and ease of scale-up. (protein-technologies.com)
PTL offers a high-level algal expression service from research to industrial grade recombinant proteins with high growth rates. (protein-technologies.com)
PTL can offer a novel Agrobacterium infiltration-mediated transient expression for a rapid and highly efficient method for protein expression in plants. (protein-technologies.com)
Enzymatic degradation of Fumonisin B1 (FB(1)) through decarboxylation has attracted much attention, whereas application of FB(1) carboxylesterase in detoxification requires more effective expression of the recombinant carboxylesterase. (inra.fr)

Upregulation of several target genes has improved recombinant protein titers and yields. (tugraz.at)
In Phase I of this project a strategy was developed and tested using the model protein alpha 1-antitrypsin for overcoming bottlenecks in yeast secretion that result in less than optimal yields. (sbir.gov)
Furthermore, translation activity, correlating to the obtained secreted recombinant protein yields, selected transcript levels and total protein content were higher in the engineered cells. (boku.ac.at)

Yeasts have many advantages as biotechnological producers relative to bacteria, however, no recombinant producers of bacterial antibiotics in yeasts are known. (biomedcentral.com)

Recent studies have revealed that the amino acid sequence does not guarantee correct folding of proteins. (biomedcentral.com)

When biopharmaceuticals are produced with one of these systems, process-related impurities termed host cell proteins also arrive in the final product in trace amounts. (wikipedia.org)

Yeast recombinant producers of bacterial antibiotics aminoriboflavin and roseoflavin were constructed and evaluated. (biomedcentral.com)

Translational regulation played an important role in the correct folding of heterologous proteins to form bioactive conformations during biogenesis. (biomedcentral.com)
The proteins present in bioactive fractions were characterised and quantified using Label-free protein quantification method. (biomedcentral.com)

The function of a protein is determined by the number and sequence of amino acids, which controls the three-dimensional structure of the resulting folded polypeptide. (frontiersin.org)
Based on that, the objective of this project will be the generation of recombinant proteins based in the primary sequence of CS proteins expressed by pre-erythrocytes forms of P. vivax. (fapesp.br)
Sequence analysis revealed that rFg14-3-3e protein had 100% identity to 14-3-3 protein from Fasciola hepatica . (biomedcentral.com)
A cDNA sequence encoding the sequence of Hirudin was constructred and used to recombinantly synthesize the protein. (enquirebio.com)

A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antikoerper-online.de)

Overexpression of closed-loop factors alone or in combination increased titers of different heterologous proteins by up to 3-fold in fed-batch processes. (boku.ac.at)

After that, we independently downregulated FBLN2 and NPR3 over seven days of osteogenic differentiation, and we performed quantitative proteomics analysis to determine how different proteins were regulated in knockdown vs. control cells. (bvsalud.org)

Angiopoietin-like proteins stimulate HSPC development through interaction with Notch receptor signaling. (stembook.org)
Estrogen Activation of G protein-coupled Estrogen Receptor 1 Regulates Phosphoinositide 3-kinase and mTOR Signaling to Promote Liver Growth in Zebrafish and Proliferation of Human Hepatocytes. (stembook.org)
The SARS-CoV-2 enters the cells via the human angiotensin-converting enzyme 2 (ACE2) receptor with the use of its spike protein 's receptor-binding domain (RBD). (news-medical.net)
The researchers paired Baylor's SARS-CoV-2 RBD recombinant protein formulation vaccine with IDRI's aluminum-based formulation of 3M's Toll-like receptor 7 and 8 agonist 3M-052 (3M-052/Alum) to enhance the immune response against SARS-CoV-2, increasing the vaccine's efficacy against COVID-19. (news-medical.net)

Recombinant A. terreus FaeD (AtFaeD) showed broad specificity towards synthetic methyl and ethyl esters, and released ferulic acid from plant biomass substrates, demonstrating its true FAE activity and interesting features as potential biocatalyst. (inrae.fr)

Global decrease in the translation elongation speed by RP deletion enhanced co-translational folding efficiency of nascent chains and decreased protein aggregates to improve heterologous protein yield. (biomedcentral.com)

However, the involvement of this protein in parasite pathogenesis remains elusive and its effect on the functions of innate immune cells is unknown. (biomedcentral.com)
This methodology is predicated on metabolic labeling of a clickable analog of palmitic acid by parasitic cells, adopted by CuAAC (Copper-catalyzed Alkyne-Azide Cycloaddition response) Click on Chemistry to render palmitoylated proteins fluorescent. (yeastevolution.com)
Some viruses possess an outer envelope, and the outer capsid is composed of major capsid protein (MCP), which appears to be highly conserved among the family and possesses surface binding sites interacting with the surfaces of host's cells [ 2 ]. (biomedcentral.com)
A protein fraction, mainly containing major royal jelly proteins 2 (MRJP2), MRJP3 and MRJP7, stimulated proliferative and migratory activities in HaCaT cells without visible cytotoxicity. (biomedcentral.com)

The greater dexterity of S. cerevisiae also brings critical advantages to protein folding and upstream adjustments, enabling optimisation to reduce downstream processing steps. (phenotypeca.com)

During her post-doctoral training, she examined structure-function relationships for SEC14p, a yeast phospholipid transfer protein involved in the late stages of the secretory pathway. (bradley.edu)

Addition of IPTG (a lactose analog) activates the lac promoter and causes the bacteria to express the protein of interest. (wikipedia.org)

To date, major royal jelly proteins (MRJPs) consisting of nine members (MRJP1-9) with molecular weight from 49 to 87 kDa have been identified in royal jelly and taken up 83-90% of its total protein content [ 5 ]. (biomedcentral.com)

A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antikoerper-online.de)

Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)

These showed a clear downregulation of genes related to protein translation with decreasing growth rates, thus revealing the yeast translation machinery as cellular engineering target. (boku.ac.at)
This is due to the delay of 60S subunit assembly by deleting non-essential ribosomal protein genes or 60S subunit processing factors, thus globally decreased the translation elongation speed and improved the co-translational folding, without perturbing the relative transcription level and translation initiation. (biomedcentral.com)

Recombinant Hepatitis B virus core antigen, amino acids 1-183. (bio-rad-antibodies.com)

The technology targets specific variations of critical locations in the genome - otherwise known as quantitative trait loci (QTL) - that functionally drive key aspects of protein manufacture. (phenotypeca.com)
Until recently, old patents kept S. cerevisiae off-limits for certain aspects of recombinant protein manufacture. (phenotypeca.com)

In last the 12 years, we generated recombinant proteins based of diverse immunodominant antigens of erythrocytes forms of P. vivax. (fapesp.br)

During this time, she worked with Dr. William A. Dunn, Jr. on the structure-function relationship of Vac8p, a vacuolar protein involved in the membrane altering steps of regulated autophagy in yeast. (bradley.edu)
Understanding the structure-function relationships for membrane-associated proteins involved in protein-trafficking events is Dr. Fry's primary research interest. (bradley.edu)
Specifically, her lab group has been identifying structural motifs in the Vac8 protein that are important for membrane association, protein-protein interactions, and vesicle fusion events occurring during the autophagy of peroxisomes in response to glucose adaptation. (bradley.edu)
Affinity tags can reduce merohedral twinning of membrane protein crystals. (lu.se)

Many other proteins are used as diagnostics or research reagents. (frontiersin.org)

Recombinant human ErB2 / Her2 (1129-ER) blocks anti-ErbB2 mediated inhibition of SK-BR-3 human breast cancer proliferation. (novusbio.com)
Homology modelling revealed 65% identity between rFg14-3-3e and human 14-3-3 protein YWHAE. (biomedcentral.com)
Analysis of the culture supernatant revealed that the recombinant human CatC was secreted as a 58-kDa molecule, indicating that human CatC was accumulated in the culture supernatant as proform composed of the residual propart, the activation peptide, and the heavy and light chains. (iyte.edu.tr)

However, these methods are successful only for a small fraction of proteins, and intensive trials and modifications are often necessary. (biomedcentral.com)
Conventional methods such as rational strain engineering and mutagenesis are not equipped to compete with QTL technology when multi-parameter optimisation is required to create a premium recombinant protein manufacturing process tailored for its market. (phenotypeca.com)

Palmitoylation refers back to the modification of the cysteine thiols in proteins by fatty acids, mostly palmitic acid, via 'thioester bond' formation. (yeastevolution.com)

Annotation of Selaginella moellendorffii major intrinsic proteins and the evolution of the protein family in terrestrial plants. (lu.se)

Anatomy4

Organisms13

Chemicals and Drugs31

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes29

Disciplines and Occupations1

Technology, Industry, Agriculture3

Information Science4

Health Care2

Pastoris47

Phaffii1

Komagataella1

Strains7

High levels of recombinant1

Extracellular4

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Purification7

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Production18

Saccharomyces1

Therapeutic2

Eukaryotic5

20231

Biotechnology1

Antibodies3

Soluble4

Secretion signal1

Vaccines1

20221

Expression13

Yields3

Biotechnological1

Correct folding of1

Host cell proteins1

Bacterial1

Bioactive2

Sequence4

Mammalian1

Titers1

Different proteins1

Receptor4

Specificity1

Heterologous protein1

Cells4

Advantages1

Secretory pathway1

Bacteria1

Content1

Glycosylation1

Post-translational1

Genes2

Amino1

Manufacture2

Antigens1

Membrane4

Reagents1

Human3

Methods2

Cysteine1

Annotation1