• Protein production Gene expression Protein purification Host cell protein Young CL, Britton ZT, Robinson AS (May 2012). (wikipedia.org)
  • In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization). (antibodies-online.com)
  • In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. (antibodies-online.com)
  • Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. (antibodies-online.com)
  • Purified (unconjugated) Thermo Scientific Pierce Recombinant Protein G is useful as the basis for preparing various kinds of probes or affinity media for detection or purification of rabbit and human antibodies, especially IgG isotypes, in immunoassays and antibody purification protocols. (thermofisher.com)
  • Because Protein G has greater affinity than Protein A for most mammalian IgGs, it may be used for the purification of mammalian IgGs that do not bind well to Protein A. Protein G binds with significantly greater capacity than Protein A to several IgG subclasses such as human IgG3, mouse IgG1 and rat IgG2a. (thermofisher.com)
  • Therapeutic proteins (e.g., antibodies, vaccines, enzymes, cytokines, and growth factors) account for almost half of this market, followed by industrial proteins (e.g., technical enzymes) and research reagents (e.g., antibodies for protein detection and purification) ( Markets and Markets, 2017 ). (frontiersin.org)
  • Market growth has been supported by advances in recombinant protein production technologies, including the engineering of expression hosts, the optimization of upstream cultivation (e.g., bioreactor design, nutritional, and physical parameters), and the development of more efficient protein extraction and purification methods. (frontiersin.org)
  • In the current study, three of the cell wall-associated antigens (arbitrarily designated as antigen A through C) were characterized for their immunogenicity potential via recombinant expression and purification using E. coli pET30a expression system. (cdc.gov)
  • Randox Biosciences is a world-leading primary manufacturer of high quality monoclonal and polyclonal antibodies, antibody fragments and human recombinant proteins. (randox.com)
  • Randox Biosciences offers an extensive portfolio of over 900 products for a multitude of R&D applications including more than 500 monoclonal and polyclonal antibodies and over 140 human recombinant proteins as well as antibody fragments, buffers & diluents and plasma. (randox.com)
  • Our custom development service, which encompasses antibodies and human recombinant proteins has achieved tremendous success within the biopharmaceutical and diagnostic industries. (randox.com)
  • Key components of our custom service are our range of monoclonal antibodies as well as our recombinant antibody fragments (sdAbs, scFvs, fAbs). (randox.com)
  • Here, we describe a new method (immuno-SILAC) for the absolute quantification of proteins in complex samples based on polyclonal antibodies and stable isotope-labeled recombinant protein fragments to allow affinity enrichment prior to mass spectrometry analysis and accurate quantification. (nih.gov)
  • Epitope mapping revealed that a majority of the polyclonal antibodies recognized multiple linear epitopes, and based on these results, a semi-automated method was developed for peptide enrichment using polyclonal antibodies immobilized on protein A-coated magnetic beads. (nih.gov)
  • A protocol based on the simultaneous multiplex capture of more than 40 protein targets showed that approximately half of the antibodies enriched at least one functional peptide detected in the subsequent mass spectrometry analysis. (nih.gov)
  • Here, we show that we were able to use small amounts of antibodies (50 ng per target) in this manner for efficient multiplex analysis of quantitative levels of proteins in a human HeLa cell lysate. (nih.gov)
  • The results suggest that polyclonal antibodies generated via immunization of recombinant protein fragments could be used for the enrichment of target peptides to allow for rapid mass spectrometry analysis taking advantage of a substantial reduction in sample complexity. (nih.gov)
  • The company also offers a variety of customized services, mainly focusing on recombinant production of antigens and antibodies. (the-scientist.com)
  • Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antikoerper-online.de)
  • Recombinant therapeutic antibodies and proteins market is expected to witness significant growth over the forecast period, attributed to rising advancements in the development of recombinant therapeutic drugs. (medgadget.com)
  • Increasing focus on recombinant therapeutics for drug development is expected to drive growth of the recombinant therapeutic antibodies and proteins market over the forecast period. (medgadget.com)
  • The increasing demand for recombinant therapeutic antibodies and proteins to improve the existing expression systems or developing novel approaches, in order to cater to growing demand for new therapeutics is expected to drive the market growth over the forecast period. (medgadget.com)
  • Furthermore, recombinant therapeutic antibodies and proteins market is expected to exhibit high growth, attributed to increasing adoption of protein-engineering technologies and government support for its development. (medgadget.com)
  • Within the past 2 years, investigational peptide- and recombinant protein-based serologic assays that can more easily differentiate the antibodies to HTLV-I and HTLV-II have been developed (8,9). (cdc.gov)
  • Our customisation service has proven incredibly successful in the research and development industries allowing access our expertise to obtain the antibody and protein most relevant to your line of work. (randox.com)
  • We took advantage of the antibody resources publicly available from the Human Protein Atlas project covering more than 80% of all human protein-coding genes. (nih.gov)
  • Sino Biological, Inc. (SZSE: 301047) has recently deposited reagents for the Omicron variant, including recombinant proteins to Spike and Nucleocapsid, and antibody products to BEI Resources, a central repository for reagents to support infectious disease research. (the-scientist.com)
  • The company specializes in recombinant antigen production and antibody development. (the-scientist.com)
  • CusabioRibosome-inactivating protein bryodin I Antibody is Available at Gentaur Genprice with the fastest delivery.Online. (joplink.net)
  • The purity of Mouse IL-11 Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant mIL-11 and staining with Coomassie Blue. (cellsignal.com)
  • The purity of the protein is >90% by SDS-PAGE. (activemotif.com)
  • Protein purity is determined by scanning densitometry of Coomassie Blue stained protein on a 4-20% gradient polyacrylamide gel. (cytoskeleton.com)
  • Gelsolin protein purity determination. (cytoskeleton.com)
  • Equad, envelope protein with 4 amino acid changes. (cdc.gov)
  • Equad proteins are the envelope proteins from DENV with 4 amino acid changes (T76R, Q77E, W101R, and L107R). (cdc.gov)
  • BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. (novusbio.com)
  • The tertiary structures of these proteins are very similar although their amino acid compositions are significantly different. (thermofisher.com)
  • Furthermore, this research presented a framework for identifying, analysing and understanding the effect of the uptake of individual amino acid towards recombinant protein yield. (bl.uk)
  • Each of these class I proteins contains a series of conserved 125-150 amino acid repeat motifs. (cytoskeleton.com)
  • SARS-CoV-2 B.1.1.7 Spike Protein S1, amino acid Val16-Arg685 (Accession # QHD43416.1), with (HV69-70del, Y144del, N501Y, A570D, P681H) and a C-terminal 8-His tag was expressed in CHO cells. (biolegend.com)
  • The 678 amino acid recombinant protein has a predicted molecular mass of approximately 76 kD. (biolegend.com)
  • A gene was identified (1229 bp) with a putative open reading frame (204 bp) encoding a 67-amino-acid protein exhibiting several characteristic features of MT proteins, including 16 cysteine residues (24%) and only one aromatic residue. (portlandpress.com)
  • Although the protein sequence showed high identity with plant and invertebrate MTs, it contained a unique 'linker ' region (14 amino acid residues) between the two putative metal-binding domains which contained no cysteine residues. (portlandpress.com)
  • The demand for recombinant proteins is therefore rising steadily, with a market valued at US$1.654 billion in 2017 predicted to reach US$2.850 billion by 2022 ( Markets and Markets, 2017 ). (frontiersin.org)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
  • The tyrosinase‐induced polymerization of peptides offers facile access to artificial mussel foot protein analogues. (wikipedia.org)
  • The use of amino acids, proteins and peptides is not considered to have any environmental impact. (janusinfo.se)
  • According to the European Medicines Agency guideline on environmental risk assessments for pharmaceuticals (EMA/CHMP/SWP/4447/00), vitamins, electrolytes, amino acids, peptides, proteins, carbohydrates, lipids proteins, vaccines and herbal medicinal products are exempted because they are unlikely to result in significant risk to the environment. (janusinfo.se)
  • Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
  • In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. (antikoerper-online.de)
  • The molecular weight of the recombinant histone is 15,306 Daltons. (activemotif.com)
  • The mature protein has an approximate molecular weight of 24 kDa. (invivogen.com)
  • Recombinant BPTF / FALZ (2791-2911) protein that includes amino acids 2791-2911 of human FALZ protein (accession number NP_872579.2) was expressed in E. coli and contains an N-terminal His-Tag and C-terminal FLAG-Tag with a molecular weight of 20.3 kDa. (activemotif.com)
  • 100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined. (novusbio.com)
  • The function of a protein is determined by the number and sequence of amino acids, which controls the three-dimensional structure of the resulting folded polypeptide. (frontiersin.org)
  • Increased mRNA expression of a laminin-binding protein in human colon carcinoma: complete sequence of a full-length cDNA encoding the protein. (joplink.net)
  • The concentration of our recombinant proteins is measured using the absorbance at 280nm. (antibodies-online.com)
  • Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. (activemotif.com)
  • Envelope protein of the hepatitis B virus marketed as Engerix-B by SmithKline Beecham HPV Vaccine proteins Polyphenol oxidases (PPOs): These include both catechol oxidases and tyrosinases. (wikipedia.org)
  • Split-Dose Administration Enhances Immune Responses Elicited by a mRNA/Lipid Nanoparticle Vaccine Expressing Respiratory Syncytial Virus F Protein. (invivogen.com)
  • Huvet Bio Inc. is developing a COVID-19 vaccine being using a sub-unit method with a high safety profile that uses proteins instead of viruses or bacteria. (who.int)
  • Fluzone High-Dose Quadrivalent inactivated risk of developing serious complications from flu vaccine, FluBlok Quadrivalent recombinant flu, compared with young, healthy adults. (cdc.gov)
  • Cell surface HABP1 binds a wide range of extracellular molecules, including hyaluronan, vitronectin, complement component C1q, HMW kininogen, and bacterial and viral proteins (2, 6 - 9). (rndsystems.com)
  • Thus this paper describes, for the first time, an MT gene identified from macroalgae which is induced by copper exposure and whose encoded protein product binds cadmium and copper. (portlandpress.com)
  • There will be a focus on the comparative physiology of microbial and higher eukaryotic platforms for protein production. (fems-microbiology.org)
  • The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)
  • The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
  • The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. (lu.se)
  • Recombinant Lucia Luciferase Protein is a positive control for luciferase reporter assays. (invivogen.com)
  • A dilution series of the recombinant SEAP protein can also be used to determine the linear range of the assays. (invivogen.com)
  • Recombinant BPTF / FALZ (2791-2911) is suitable for use in binding assays, inhibitor screening, and selectivity profiling. (activemotif.com)
  • This recombinant protein can be used for biological assays. (prosci-inc.com)
  • The following product was used in this experiment: Human MAP4K5 (KHS1), GST Tag Recombinant Protein from Thermo Fisher Scientific, catalog # PV3682. (thermofisher.com)
  • 21955. Immobilized Recombinant Mouse CRTAM Fc Chimera (Catalog # 3687-CR) at 2 µg/mL (100 µL/well) can bind Recombinant Mouse IGSF4A/SynCAM1 with a linear range of 0.2-10 µg/mL. (rndsystems.com)
  • Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. (cellsignal.com)
  • Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl with BSA as a carrier protein. (novusbio.com)
  • CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)
  • Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
  • A: Cusabio protein product does not contain carrier protein or other additives (such as bovine serum albumin (BSA), human serum albumin (HSA) and sucrose, and when freeze-dring the lowest salt content solution often does not form a white grid structure, but a trace amount of protein deposit within the tube, forming a thin transparent or invisible protein layer. (stratech.co.uk)
  • Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. (biolegend.com)
  • Serial dilutions of Mouse IL-11 Recombinant Protein were added to B9 cells. (cellsignal.com)
  • Recombinant Protein;Cynomolgus LAG-3 / CD223 Protein, Mouse IgG2a Fc Tag;100UG;Cynomolgus LAG-3, Mouse IgG2a Fc tag (LA3-C52A0) is expressed from human 293 cells (HEK293). (fishersci.com)
  • Human PRB1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade. (antibodies-online.com)
  • Over the last three decades, the expression of recombinant proteins in plants and plant cells has been promoted as an alternative cost-effective production platform. (frontiersin.org)
  • There are also several platforms based on plants and plant cells, but these have not been included in the latest market studies, indicating they have not yet commanded a significant share of commercial protein production capacity. (frontiersin.org)
  • Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)
  • This monomeric protein is expressed in CHO cells. (invivogen.com)
  • Several expression systems for recombinant protein production, essentially cells or whole organisms are currently in use today. (bl.uk)
  • mRNA vaccines use messenger RNA, which gives your cells instructions for how to make a protein (or piece of a protein) of the germ. (medlineplus.gov)
  • Viral vector vaccines use genetic material, which gives your cells instructions for making a protein of the germ. (medlineplus.gov)
  • Protein quantitation was performed using the Precision Red Protein Assay Reagent (Cat. (cytoskeleton.com)
  • When recombinant SARS-CoV-2 B.1.1.7 Spike Protein S1 is immobilized at 2 μg/mL, recombinant human ACE2-Fc Chimera (Cat. (biolegend.com)
  • The samples were tested in a binding assay with recombinant human ACE2-Fc Chimera. (biolegend.com)
  • Enhances transcriptional repression by coordinating the increase in H3K9me, the decrease in histone H3 'Lys-9 and 'Lys-14' acetylation (H3K9ac and H3K14ac, respectively) and the disposition of HP1 proteins to silence gene expression. (abcam.com)
  • This protein belongs to the FET family of RNA-binding proteins which have been implicated in cellular processes that include regulation of gene expression, maintenance of genomic integrity and mRNA/microRNA processing. (origene.com)
  • Here, we discuss the strengths of plant expression systems for specific applications, but mainly address the bottlenecks that must be overcome before plants can compete with conventional systems, enabling the future commercial utilization of plants for the production of valuable proteins. (frontiersin.org)
  • Even so, plants as an alternative expression platform offer unique advantages, particularly when target proteins are difficult to produce in conventional systems, require specific qualitative properties such as particular glycan profiles, and/or must be produced on a larger scale in response to urgent demand. (frontiersin.org)
  • Bromodomains recognize acetylated histone lysine residues and function as 'readers' of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. (activemotif.com)
  • Recently, research into recombinant protein production revealed a more attractive expression system based on the microalgae, C. reinhardtii, for significant savings in cost and production of correctly folded recombinant proteins. (bl.uk)
  • Using the expression of E. coli β-glucuronidase (gus) in C. reinhardtii chloroplast, the overall aim of the project was to address if the low recombinant gus yield in C. reinhardtii was due to limitations that affect growth and protein production, and if the fluxes for recombinant gus production were suboptimal (limiting). (bl.uk)
  • No detectable protein burden was observed for expression of recombinant gus in autotrophic and mixotrophic conditions, but protein burden was significant in heterotrophic condition (15 - 18% reduction in growth rate). (bl.uk)
  • Medicinal and industrial usage of recombinant protein in drug manufacturing is rising, coupled with increasing demand for various protein expression systems and other related high-tech systems, which are expected to drive the market growth. (medgadget.com)
  • Members of the board and scientific committee of PPS, Sabine Suppmann and Kim Remans, respectively are among the authors of a new review in Star Protocols about expression systems for recombinant protein production. (lu.se)
  • The overview is based on a survey with 60 leading experts in protein production about their experiences and practices in using different expression systems for recombinant protein production. (lu.se)
  • It helps researches to choose expression system based on cost-effectiveness, speed, and properties like protein folding, post-translational modifications and secretion. (lu.se)
  • In many cases, recombinant human proteins have replaced the original animal-derived version used in medicine. (wikipedia.org)
  • The prefix "rh" for "recombinant human" appears less and less in the literature. (wikipedia.org)
  • This product is manufactured by BioVision, an Abcam company and was previously called 4580 BMP-14 (GDF-5/CDMP-1), human recombinant. (abcam.com)
  • Haptoglobin Human Recombinant produced in E. coli is a single polypeptide chain containing 352 amino acids (19-347) and having a molecular mass of 39.0 kDa. (prospecbio.com)
  • However, Protein G does not bind to human IgM, IgD and IgA. (thermofisher.com)
  • CD247 Human Recombinant produced in E. coli is a single polypeptide chain containing 136 amino acids (52-164) and having a molecular mass of 15.4kDa. (prospecbio.com)
  • Recombinant SEAP (secreted embryonic alkaline phosphatase) protein is a truncated form of human placental alkaline phosphatase that comprises 520 amino acids. (invivogen.com)
  • 10 μg lyophilized recombinant human secreted embryonic alkaline phosphatase (SEAP). (invivogen.com)
  • CD40 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids (21-193a.a) and having a molecular mass of 21.6kDa. (neuromics.com)
  • Gelsolin protein has been purified from E.coli expressing recombinant 6xHIS-tagged human gelsolin (plasma isoform). (cytoskeleton.com)
  • Recombinant lysosomal proteins are used for both research and medical applications, such as enzyme replacement therapy. (wikipedia.org)
  • Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. (prosci-inc.com)
  • To establish a nested recombinant enzyme -assisted polymerase chain reaction (RAP) technique combined with recombined mannose-binding lectin protein (M1 protein )- magnetic beads enrichment for the detection of Candida albicans (C. albicans) and Candida tropicalis (C. tropicalis) in blood samples for the early diagnosis of candidemia albicans and candidiemia tropicalis. (bvsalud.org)
  • Adzynma is the first recombinant protein product for preventive or on-demand enzyme replacement therapy for people with the blood clotting condition. (medscape.com)
  • The new agent is a purified recombinant form of the ADAMTS13 enzyme that works by replacing low levels of the deficient enzyme in patients with congenital TTP. (medscape.com)
  • IgM endpoint titers of DENV-1, DENV-2, and DENV-4 PCR-positive and NS1 antigen-positive serum samples from residents of dengue endemic countries Vietnam, Sri Lanka, Venezuela, and Brazil, 2013-2018, on DENV 1-4 Equad proteins. (cdc.gov)
  • The minimum protein concentration (µg/well) that induced T-cell proliferation was 10 (Antigen B) and 25 (antigens A and C) whereas the lowest test concentration (5 µg/well) of each antigen could induce significant amounts of IFN-gamma, albeit to a varying extent (250-300 pg/ml). (cdc.gov)
  • The coronavirus spike (S) glycoprotein is a class I viral fusion protein on the outer envelope of the virion that plays a critical role in viral infection by recognizing host cell receptors and mediating fusion of the viral and cellular membranes. (biolegend.com)
  • Nevertheless, we suggest to store at -20 ℃ upon receipt of our products, to ensure 100% activity of the protein. (stratech.co.uk)
  • Why our protein products are almost invisible in pipes? (stratech.co.uk)
  • Citations will be maintained to the appropriate protein classes and the general heading 'Recombinant Proteins. (bvsalud.org)
  • Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. (origene.com)
  • Recombinant SEAP protein is purified by affinity chromatography. (invivogen.com)
  • Recombinant Histone H3S10ph (EPL) has been generated using the patented Expressed Protein Ligation (EPL) technology. (activemotif.com)
  • The ligation reaction maintains the native histone bonds, and the resulting recombinant protein more closely mimics natural histones. (activemotif.com)
  • The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). (activemotif.com)
  • Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points. (activemotif.com)
  • Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro . (activemotif.com)
  • Recombinant Histone H3 phospho Ser10 (H3S10ph) proteins are generated using expressed protein ligation (EPL) technology. (activemotif.com)
  • The purified protein is subsequently ligated to a N-terminal histone tail peptide containing phosphorylated serine 10 via a native peptide bond. (activemotif.com)
  • Recombinant Histone H3 phospho Ser10 tested by SDS-PAGE gel. (activemotif.com)
  • SDS-PAGE analysis of 2 µg Recombinant Histone H3 phospho Ser10 (lane 1) and 1 µg Recombinant Histone H3 phospho Ser10 (lane 2). (activemotif.com)
  • SDS-PAGE gel of Recombinant Histone H3 phospho Ser10. (activemotif.com)
  • 3 µM histone peptide H4K5/8/12/16(4Ac) was incubated with BPTF / FALZ (2791-2911) protein in reaction buffer including 50 mM HEPES-NaOH pH 7.0, 0.1% BSA for 1 hour at room temperature. (activemotif.com)
  • The S glycoprotein is synthesized as a precursor protein consisting of ~1,300 amino acids that is then cleaved into an amino (N)-terminal S1 subunit (~700 amino acids) and a carboxyl (C)-terminal S2 subunit (~600 amino acids). (biolegend.com)
  • Recombinant SARS-CoV-2 B.1.1.7 Spike Protein S1 was aliquoted in PBS at 0.2 mg/mL. (biolegend.com)
  • This product is an active protein and may elicit a biological response in vivo, handle with caution. (abcam.com)
  • The dissolving solution indicated in the product datasheet is the liquid which can completely dissolve the cytokine or recombinant protein. (stratech.co.uk)
  • If the pH and ionic strength of the user's solution are inconsistent with the product datasheet, in most case, this will cause that the cytokine or recombinant protein can't be dissolved completely or even can't be dissolved, then the prepared cytokine or recombinant protein will absolutely be lack or loss of activity. (stratech.co.uk)
  • 3M™ Polisher ST is a fully encapsulated single-use anion exchange (AEX) product intended to reduce host cell protein impurities, viruses, and other negatively charged contaminants in flow-through polishing chromatography. (3m.com.au)
  • MyBiosourceProduct Short Name: [68 kDa Neurofilament]Product Name Synonyme: [Neurofilament 68 kDa protein]Other Names: N/AProduct Gene Name: N/AProduct. (biocheminfo.org)
  • How to properly dissolve recombinant protein product? (stratech.co.uk)
  • These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins (1, 2). (novusbio.com)
  • Reconstitute to a concentration of 0.1-1.0 mg/ml in water containing BSA (50 µg BSA per 1 µg of protein). (abcam.com)
  • Mouse IL-11 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. (cellsignal.com)
  • The concentration of the protein is calculated using its specific absorption coefficient. (antibodies-online.com)
  • Please refer to the lot-specific TDS you will receive with your order for the lot-specific buffer contents and protein concentration. (activemotif.com)
  • Recombinant KISS1 protein was expressed in E.coli and purified by using conventional chromatography techniques. (novusbio.com)
  • Approximately 44% more IgG from rat serum bound to Protein G when Protein G Binding Buffer was used as compared with 20 mM Tris Buffer, pH 7.5. (thermofisher.com)
  • The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (biocheminfo.org)
  • After reconstitution with of nanopure water, the protein will be in the following buffer: 10 mM Tris pH 7.5, 10 mM NaCl, 0.1 mM MgCl2, 1% (w/v) sucrose, and 0.1% (w/v) dextran. (cytoskeleton.com)
  • Nevertheless, some plant-derived proteins for research or cosmetic/pharmaceutical applications have reached the market, showing that plants can excel as a competitive production platform in some niche areas. (frontiersin.org)
  • Moreover, pharmaceutical recombinant protein is increasingly adopted for the treatment of diseases such as cancer, diabetes, anemia, and hepatitis. (medgadget.com)
  • However, the advent of recombinant protein technology for the production of most pharmaceutical proteins has made this distinction obsolete. (bvsalud.org)
  • Note that defined pharmaceutical preparations of individual recombinant proteins, e.g., interferon alfa-2a, will be maintained in the supplemental concept record. (bvsalud.org)
  • BPTF / FALZ (2791-2911) protein was run on a 12% SDS-PAGE gel and stained with Coomassie Blue. (activemotif.com)
  • Recombinant BPTF / FALZ (2791-2911) protein gel, stained with Coomassie Blue. (activemotif.com)
  • There are inconsistencies in reported binding properties of IgG to Protein G. Variations in isolation and manufacturing methods for Protein G may affect IgG binding, partially because there are differing numbers of IgG-binding sites on various sources of Protein G. Binding studies have been performed using native Protein G and several different recombinant forms. (thermofisher.com)
  • Our experts in the lab will ensure that you receive a correctly folded protein. (antibodies-online.com)
  • When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. (antibodies-online.com)
  • The full-length protein is then repurified prior to lyophilization. (activemotif.com)
  • Why does protein need lyophilization? (stratech.co.uk)
  • What influences do the lyophilization have on proteins? (stratech.co.uk)
  • A: The proteins are very sensitive to heat, protein lyophilization can make most of the activity retain, improve protein stability and extend the storage time, meanwhile reduce shipping costs. (stratech.co.uk)
  • Lyophilization may cause partial loss of protein activity, aggregation and other degenerative problems. (stratech.co.uk)
  • Why should we add protective agent to the protein solution before lyophilization? (stratech.co.uk)
  • mannitol is also a universal applied lyoprotectant and fillers, which can reduce the aggregation of certain proteins after lyophilization. (stratech.co.uk)
  • Plant cell culture was used to produce FDA-approved glycosylated lysosomal protein-drug, and additional drug candidates. (wikipedia.org)
  • The bioactivity of recombinant mIL-11 was determined in a B9 cell proliferation assay. (cellsignal.com)
  • This conference series aims at offering a comprehensive overview of current challenges and innovative solutions in recombinant protein production across different cell factories. (fems-microbiology.org)
  • The protein encoded by this gene is the fourth major glycoprotein of the platelet surface and serves as a receptor for thrombospondin in platelets and various cell lines. (origene.com)
  • Since thrombospondins are widely distributed proteins involved in a variety of adhesive processes, this protein may have important functions as a cell adhesion molecule. (origene.com)
  • Protein G is a bacterial cell wall protein isolated from group G Streptococci. (thermofisher.com)
  • DNA sequencing of native Protein G identifies two IgG-binding domains and sites for albumin and cell surface binding. (thermofisher.com)
  • The albumin and cell surface binding domains have been eliminated from Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. (thermofisher.com)
  • A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antikoerper-online.de)
  • IGSF4A is a 75 kDa immunoglobulin superfamily protein that mediates calcium-independent adhesive interactions, either homophilic or heterophilic with CRTAM (class I-restricted T cell-associated molecule), Necl-1 (SynCAM3) or Nectin-3 (1 - 4). (rndsystems.com)
  • Using FBA modelling, model verifications and corrections, a strategy that significantly increased the yield of recombinant gus in each cell (~2 fold) was identified. (bl.uk)
  • Vol 8, 2319) on this newly-recognized mycobacterial pathogen led to the first identification of 33 immuno-reactive proteins, comprising of 4 secretory, 6 cell wall-associated, 11 membranous, and 12 cytosolic proteins. (cdc.gov)
  • The 9th Conference on Recombinant Protein Production (RPP9) will build on the successes of previous meetings, which have attracted steadily increasing numbers of researchers from both academia and the bioprocessing industry. (fems-microbiology.org)
  • Made in Germany - from design to production - by highly experienced protein experts. (antibodies-online.com)
  • Although plant systems are now gaining widespread acceptance as a platform for the larger-scale production of recombinant proteins, there is still resistance to commercial uptake. (frontiersin.org)
  • The focus of our protein production platform is the provision of recombinant proteins for research use, where generally only small quantities are required, in the range of tens of micrograms to a few hundred milligrams. (lu.se)