Recombinant protein protein healthy marrow. Medical search. Frequent questions

IL-11 is a member of a family of human growth factors and is being produced in the bone marrow of healthy adults. (wikipedia.org)

Significant reductions in the parasite load in liver, spleen, bone marrow and draining lymph nodes were found in these animals. (cambridge.org)
However, for VL diagnosis, aspirates of lymph nodes, bone marrow or spleen need to be obtained, being both risky and painful for patients. (biomedcentral.com)

Recommendations on immunization following bone marrow transplantation will be published in a separate ACIP statement. (cdc.gov)

Red cell production in the bone marrow is regulated by erythropoietin, a growth hormone produced in the kidney. (hospitalpharmacyeurope.com)
Anemia in pregnancy and the puerperium should be treated according to a staged regimen, administering either iron alone or in combination with an off-label use of human recombinant erythropoietin in selected patients. (karger.com)
Normally kidneys produce a hormone called erythropoietin (EPO), which stimulates red blood cell production in the bone marrow. (onetrackhealth.com)
1. Erythropoiesis‑stimulating agents (ESAs) - manufactured recombinant proteins that work by stimulating the bone marrow to produce red blood cells, resulting in the same biological effect as endogenous erythropoietin (EPO). (onetrackhealth.com)
Amgen revolutionized anemia treatment with the development of Epoetin alfa, a recombinant erythropoietin (a protein that stimulates the production of oxygen-carrying red blood cells). (amgen.com)

The component iron is recycled by carrier molecules (such as transferrin) to the body's insoluble iron store in the liver, or back to the bone marrow as soluble iron to be used once again in making new haemoglobin. (hospitalpharmacyeurope.com)
The transmembrane form can be cleaved from the membrane, generating a soluble protein fragment. (wikidoc.org)

Oprelvekin is recombinant interleukin eleven (IL-11), a thrombopoietic growth factor that directly stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production. (wikipedia.org)
Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. (novusbio.com)
Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). (cusabio.com)

In this study, a Leishmania hypothetical protein, LiHyS, was evaluated regarding its antigenicity, immunogenicity and protective efficacy against visceral leishmaniasis (VL). (cambridge.org)
Thus, we considered of interest to analyze whether Leishmania PUF proteins result antigenic during visceral leishmaniasis (VL). (biomedcentral.com)
Additionally, the 10 genes of the L. infantum PUF family were cloned and used to express the Leishmania PUFs in bacteria as recombinant proteins. (biomedcentral.com)

The reactivity of hamster sera against recombinant LiPUF1 and LiPUF2 was particularly prominent, and these proteins were subsequently assayed against sera from human patients. (biomedcentral.com)

Oprelvekin is produced in Escherichia coli (E. coli) by recombinant DNA technology. (wikipedia.org)
Synthesized in E. coli, this full-length mature protein spans the 24-91aa expression region and is tag-free for maximum compatibility with your experimental setup. (cusabio.com)

Recombinant Human IL-7 GMP Protein Bioactivity GMP-grade Recombinant Human IL-7 (Catalog # 207-GMP) stimulates proliferation of PHA-activated human peripheral blood lymphocytes. (bio-techne.com)

When evaluating the immunogenicity of LiHyS, which is possibly located in the parasite's flagellar pocket, proliferative assays using peripheral blood mononuclear cells from healthy subjects or VL patients showed a high proliferative index in both individuals, when compared to the results obtained using rA2 or unstimulated cultures. (cambridge.org)
Yet, unlike single-cell genomics and transcriptomics, improvements in single-cell protein assays are lagging. (irjs.info)
Strikingly, most single-cell protein assays (for unmodified endogenous focuses on) are single-stage immunoassays, including enzyme-linked immunosorbent assays (with direct or sandwich readout) and immunocytochemistry, as well as newer immunoassay types designed to improve multiplexing using spatial barcoding15,16 or mass cytometry17. (irjs.info)

To test the effect of decreasing p53 to particular levels via RNA interference, the scientists reconstituted the blood cells of mice by first irradiating the animals to destroy their endogenous, bone marrow supply of hematopoietic stem cells, and then injected the mice with a fresh supply of hematopoietic stem cells that had been engineered through RNAi to produce low, medium, or high levels of p53. (sciencedaily.com)
Folate receptor beta (FRβ) is only detectable in placenta and limited to some hematopoietic cells of myeloid lineage in healthy people. (biomedcentral.com)

Regarding antigenicity, immunoblottings and an enzyme-linked immunosorbent assay using human and canine sera showed high sensitivity and specificity values for the recombinant protein (rLiHyS) in the diagnosis of VL. (cambridge.org)
Finally, the antigenicity of these PUF proteins was evaluated by determining levels of specific antibodies in sera from experimentally infected hamsters. (biomedcentral.com)

Our commitment to quality ensures that the Recombinant Human CCL5 protein boasts a purity of >98% as determined by SDS-PAGE and HPLC analysis. (cusabio.com)

Neutrophils isolated from the cancer-induced inflammatory microenvironment contain β-endorphin protein and are identified by the Ly6G + immune marker. (frontiersin.org)
Oral cancer cells secrete granulocyte colony stimulating factor (G-CSF), a growth factor that recruits neutrophils from bone marrow to the cancer microenvironment. (frontiersin.org)
Saline-treated male mice with HSC-3 tumors exhibited less oral cancer-induced nociceptive behavior and had more β-endorphin protein in the cancer microenvironment than saline-treated female mice with HSC-3 tumors. (frontiersin.org)
The role of the marrow microenvironment in the pathophysiology of myelodysplastic syndromes (MDSs) remains controversial. (researchtoactionforum.org)

The BLyS protein in the engineered bacteria can be as much as 50% to the bacteria's total protein content and still retains activity after a purification procedure. (wikidoc.org)

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (bio-techne.com)
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (bio-techne.com)
Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein. (novusbio.com)

The characterization of proteins involved in the regulation of gene expression in S. mansoni is of great importance for the understanding of molecular events that control morphological and physiological changes in this parasite. (cambridge.org)
B-cell activating factor ( BAFF ) also known as tumor necrosis factor ligand superfamily member 13B is a protein that in humans is encoded by the TNFSF13B gene . (wikidoc.org)
Here, we report on the clinical impact of a novel NiV-derived nonspreading replicon particle lacking the fusion (F) protein gene (NiVF) as a vaccine in three small animal models of disease. (cdc.gov)

This includes the regulation of intestinal epithelium growth (enhanced healing of gastrointestinal lesions), the inhibition of adipogenesis, the induction of acute phase protein synthesis (e.g., fibrinogen), and inhibition of macrophageal released pro-inflammatory cytokines. (wikipedia.org)
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. (rndsystems.com)

The alpha granules contain hemostatic proteins such as fibrinogen, vWf, and growth factors (eg, platelet-derived growth factor and transforming growth factors). (medscape.com)

Although mass spectrometry can measure most protein isoforms, the analytical level of sensitivity is insufficient for detection of important signalling proteins with single-cell resolution26. (irjs.info)
Among the four isoforms of FRs identified (α, β, γ, and δ), α and β isoforms of FR are glycosylphosphatidylinositol (GPI)-anchored proteins with two N-glycosylation sites, and both have high affinity (K D of approximately 1 nM) for folate [ 2 ]. (biomedcentral.com)

RNA-binding proteins of the PUF family share a conserved domain consisting of tandemly repeated 36-40 amino acid motifs (typically eight) known as Puf repeats. (biomedcentral.com)

Recombinant Human IL-7 GMP Protein SDS-PAGE 1 μg/lane of GMP-grade Recombinant Human IL-7 (Catalog # 207-GMP) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa. (bio-techne.com)
Recombinant Human IL-7 GMP Protein Mass Spectrometry MALDI-TOF analysis of GMP-grade Recombinant Human IL-7 (Catalog # 207-GMP). (bio-techne.com)

Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2) . (rndsystems.com)

Still, the analytical level of sensitivity of slab-gel western blotting requires pooling of cells to accomplish detectable protein concentrations, which obscures important CTC-to-CTC protein manifestation level variance. (irjs.info)

Among the therapies tested in MPS IIIA, one is the delivery of the therapeutic agent directly to the CNS by periodic administrations of recombinant protein to the cerebrospinal fluid (CSF) through a permanently implanted intrathecal delivery device ("type":"clinical-trial","attrs":"text":"NCT01155778″,"term_id":"NCT01155778″NCT01155778 and "type":"clinical-trial","attrs":"text":"NCT01299727″,"term_id":"NCT01299727″NCT01299727, clinicaltrials.gov). (scapca.org)
DESCRIPTION (provided by applicant): With over 300 therapeutic proteins currently in various stages of clinical trials, the road to a healthier future will require new methods for producing safer and less expensive recombinant proteins. (sbir.gov)

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (cusabio.com)

The protein has a molecular mass of approximately 19,000 g/mol, and is non-glycosylated. (wikipedia.org)
Separating proteins by molecular mass (or mobility) before the immunoassay can determine off-target, non-specific antibody binding27. (irjs.info)

Discover the versatility of our Recombinant Human CCL5 protein, an essential tool for advancing immunological research. (cusabio.com)
As such, our Recombinant Human CCL5 protein serves as a valuable tool in elucidating the molecular mechanisms underlying immune function and developing novel therapeutics for immune-related disorders [2] . (cusabio.com)
Erythropoietins - commonly called "epoietins" or "EPOs" - are recombinant human proteins that are licensed for the treatment of cancer-�chemotherapy-induced anaemia. (hospitalpharmacyeurope.com)
Human RBCs are formed in the bone marrow and have an average lifespan of up to 120 days. (hospitalpharmacyeurope.com)
It's no secret to readers here that DrJohn and DrGeorge have day jobs as stem cell researchers, both in the lab and the clinic, and have developed skincare products whose hero ingredients (human growth factors, cytokines, mRNAs,miRNAs, etc) derive from stem cells on permanent loan from bone marrow of young adult humans. (barefacedtruth.com)
Functional recombinant FRβ protein was produced in insect cells and used as antigen to isolate a mAb, m909, from a human naïve Fab phage display library. (biomedcentral.com)

In the current study, phage-exposed mimotopes as targets against tegumentary leishmaniasis (TL) were selected by means of bio-panning cycles employing sera of TL patients and healthy subjects, besides the immune stimulation of peripheral blood mononuclear cells (PBMCs) collected from untreated and treated TL patients and healthy subjects. (cambridge.org)

Fig. 1: Radiation exposure in healthy lung tissue enhances metastasis. (nature.com)

Protein-protein interactions are important events in all biological processes, since most proteins execute their functions through large supramolecular structures. (cambridge.org)

Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. (novusbio.com)

Here we demonstrate that SMYB1 is located in the cytoplasm of cells from different life-cycle stages of S. mansoni , suggesting that this protein is probably acting in mRNA metabolism in the cytoplasm and corroborating previous findings from our group that showed its ability to bind RNA. (cambridge.org)
Their cytoplasm is filled with an iron-containing protein called haemoglobin (Hb), the substance that gives the blood its red colour. (hospitalpharmacyeurope.com)
The platelets arise from the fragmentation of the cytoplasm of megakaryocytes in the bone marrow and circulate in blood as disc-shaped anucleate particles for 7-10 days. (medscape.com)

Hemoglobin is the oxygen carrying protein found in RBCs that carries oxygen from the lungs to the tissues of the body including heart, brain and muscles which help you function normally. (onetrackhealth.com)

Red blood cells (RBCs) are created in the bone marrow and contain the oxygen carrying protein hemoglobin (Hgb), which provides oxygen to your cells and is important for normal function of your heart and brain as well as other organs. (onetrackhealth.com)

IL-7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). (bio-techne.com)
It is expressed as a membrane-bound type II transmembrane protein [2] on various cell types including monocytes , dendritic cells and bone marrow stromal cells. (wikidoc.org)

High antibody responses against rLiPUF1 and rLiPUF2 were found in sera from VL patients, but these proteins resulted also recognized by sera from Chagas' disease patients. (biomedcentral.com)
Yet, medical immunoassays (for example, immunohistochemistry) are limited to 5 proteins due to spectral imaging limitations with conventional filter units22,23 and difficulty in de-staining' cells (eliminating antibody probes). (irjs.info)
Spatially resolving proteins by size 1st allows a single antibody probe to detect multiple, unique protein forms28. (irjs.info)

Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). (novusbio.com)
Most intracellular phosphate is either complexed or bound to proteins and lipids. (medscape.com)
Several sodium-coupled transport proteins have been identified that enable intracellular uptake of phosphate by taking advantage of the steep extracellular-to-intracellular sodium gradient. (medscape.com)
Although important, the capability to multiplex and assay a wide range of protein focuses on (including intracellular signalling pathways) has been limited20. (irjs.info)
First, single-stage immunoassays have difficulty with multiplexed measurements of surface and intracellular proteins for each solitary cell21. (irjs.info)
Circulation cytometry also suffers from multiplexing shortcomings, especially with intracellular protein focuses on. (irjs.info)

Myelodysplastic syndromes (MDS) are rare bone marrow disorders that impair how well your body creates new blood cells. (healthline.com)

Progressive infiltration of Gaucher cells in the bone marrow may lead to thinning of the cortex, pathologic fractures, bone pain, bony infarcts, and osteopenia. (medscape.com)
MDS prevent your bone marrow from making the necessary level of red blood cells, which help transport oxygen throughout the body. (healthline.com)
During a transfusion, you're given blood cells from a healthy donor through an IV. (healthline.com)
Previously, we identified inducible nitric oxide synthase (iNOS) as an essential enzyme for development and reversal of smoke-induced PH and emphysema, and showed that iNOS expression in bone-marrow-derived cells drives pulmonary vascular remodelling, but not parenchymal destruction. (ersjournals.com)
Major Compact disc34+ cells from MDS marrow when cocultured with HS5 and TNF-α also underwent apoptosis. (researchtoactionforum.org)
On TCS 21311 the other hand no apoptosis was seen in Compact disc34+ cells through the marrow of healthful donors. (researchtoactionforum.org)
These studies identified PYCARD a cytoplasmatic adaptor protein consisting of a pyrin domain name (PYD) and a caspase recruitment domain name (CARD)12-16 as a central factor in myeloid cells that decided apoptosis in response to signals such as TNF-α and TRAIL. (researchtoactionforum.org)
Osteoclasts orginate from the fusion of macrophage-like cells in the bone marrow. (assaygenie.com)
Do you know that fat stem cells and bone marrow stem cells are really, really different? (barefacedtruth.com)
To intelligently employ bio-signaling molecules in one's medical practice (or one's home skincare regimen), there should be familiarity with the significant differences among the bio-signals patterns produced by adipose (fat) stem cells and bone marrow mesenchymal stem cells, and for completeness, fibroblasts. (barefacedtruth.com)
Consequently, considerable research has Geldanamycin focused on the isolation of CTCs by exploiting unique characteristics of these malignancy cells (for example, surface protein manifestation, size and deformability)2,3,4,5. (irjs.info)
In normal cells, folates are taken in through the reduced folate carrier (RFC) or proton-coupled folate transporter (PCFT), which are membrane-spanning proteins that facilitate bidirectional transportation of reduced folate across the plasma membrane and endosome membranes [ 1 ]. (biomedcentral.com)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)

Captured proteins are visualized using chemiluminescent detection reagents (Step 3) . (rndsystems.com)
After detection, the array data were quantitated to generate a protein profile (histogram). (rndsystems.com)

We describe a case in which a nongroupable meningococcal strain, myeloid and erythroid maturation without evidence of dys- which rarely causes disease in healthy persons, caused fa- plasia, aplasia, or an aberrant cell population. (cdc.gov)

In conclusion, we presented a strategy to identify new mimotopes able to induce Th1 response in PBMCs from TL patients and healthy subjects, and that were successfully used to protect against L. amazonensis infection. (cambridge.org)
3,11 Moreover, RSV infection is life-threatening to bone-marrow transplant patients. (rtmagazine.com)
Radiotherapy is one of the most effective approaches to achieve tumor control in cancer patients, although healthy tissue injury due to off-target radiation exposure can occur. (nature.com)
However, studies by Ballmaier and colleagues and Sekine and associates showed comparable or increased levels of thrombopoietin in patients with TAR compared with healthy control subjects. (medscape.com)

[5] TACI binds worst since its affinity is higher for a protein similar to BAFF, called a proliferation-inducing ligand (APRIL). (wikidoc.org)

Here, we generate a chimeric protein (SpiN) that comprises the receptor binding domain (RBD) from Spike (S) and the nucleocapsid (N) antigens from SARS-CoV-2. (bvsalud.org)
Direct measurement of multiple proteins in single-CTCs comprises a critical match to single-CTC transcriptomic and genomic studies, as well as enumeration. (irjs.info)

j Analysis of MERTK protein in osteoblast cultures from Mertk flox/flox mice treated with recombinant CRE recombinase. (medicalxpress.com)
We found that recombinant G-CSF (rG-CSF, 5 μg/mouse, intraperitoneal) significantly increased circulating Ly6G + neutrophils in the blood of male and female mice within 24 h of administration. (frontiersin.org)
Chronic rG-CSF treatment (2.5 μg/mouse, every 72 h) increased the HSC-3 recruited Ly6G + neutrophils, increased β-endorphin protein content in the tongue and attenuated nociceptive behavior in female mice with HSC-3 tumors. (frontiersin.org)

Healthy volunteers displayed an increase in von-Willebrand-factor (vWf) activity. (wikipedia.org)

According to studies, 14 ribavirin appears to interfere with messenger RNA by inhibiting viral protein synthesis. (rtmagazine.com)

Although current treatment modalities offer high levels of success for many conditions, an ideal form of therapy might consist of regenerative approaches in which diseased or necrotic pulp tissues are removed and replaced with healthy pulp tissue to revitalize teeth. (bvsalud.org)

The findings of residual amounts of viral DNA fragments referred to in the question are an expected finding and data in the literature supports that the residuals are rapidly eliminated and do not result in any expression of protein. (sanevax.org)

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (bio-techne.com)
We exposed healthy mouse lung tissue to radiation before the induction of metastasis and observed a strong enhancement of cancer cell growth. (nature.com)

The world has never seen a virus with a spike protein that is so toxic to the system, nor has the world's population seen a vaccine with so many firsts-such as being the first to use mRNA technology against an infectious virus. (jewelryon.com)

May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. (cusabio.com)

Mechanistically, SEs recruited bromodomain containing 4 (BRD4) at the site of ZBTB16 , which then bound to RNA polymerase II-associated protein 2 (RPAP2) that transported RNA polymerase II (POL II) into the nucleus. (nature.com)

The insulin receptor (IR) and JAK1 tyrosine kinases and STAT3 can serve as direct substrates for the T-cell protein tyrosine phosphatase (TCPTP). (diabetesjournals.org)

Here we generated a novel, two-sided fusion protein incorporating the extracellular domains of SIRPα and CD40L, adjoined by a central Fc domain, termed SIRPα-Fc-CD40L. (aacrjournals.org)

This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag. (cusabio.com)

Ferritin is a protein involved with iron regulation in the body. (healthline.com)
The addition and deletion of phosphate groups to enzymes and proteins are common mechanisms for the regulation of their activity. (medscape.com)

To surmount this Geldanamycin space, we recently launched a single-cell resolution western blot29 optimized for study of protein manifestation in each of thousands of single, cultured neural stem cells30 and glioblastoma cells31. (irjs.info)

Anatomy24

Organisms29

Diseases13

Chemicals and Drugs69

Analytical, Diagnostic and Therapeutic Techniques and Equipment58

Phenomena and Processes70

Disciplines and Occupations2

Technology, Industry, Agriculture3

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Produced in the bone ma1

Spleen2

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Induces3

Leishmania3

Reactivity1

Coli2

Stimulates1

Assays3

Hematopoietic2

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Purity1

Microenvironment4

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Carrier protein3

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Human6

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Enhances1

Interactions1

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Cytoplasm3

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