AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaTechnology, Industry, AgricultureInformation ScienceHealth CareGeographicals
Carbonic AcidPlants, Genetically ModifiedPlant LeavesPlant ProteinsRecombinant ProteinsPlant RootsGenes, PlantPlant ExtractsPlant ShootsPlants, MedicinalDNA, PlantPlant DevelopmentPlants, ToxicPlant CellsPlant StemsGenome, PlantArabidopsisAmino Acid SequenceRecombinant Fusion ProteinsZea maysQuantitative Trait LociChromosome MappingInbreedingEncyclopedias as TopicMolecular BiologyVeterinary MedicineLaboratoriesInsulin, Regular, PorkGrowth HormoneEnvironmental HealthUnited States National Aeronautics and Space AdministrationNational Library of Medicine (U.S.)Government AgenciesEuropean UnionUnited States
ProteinsMammalianMicroorganismsPurificationTransgenicAntibodiesProtein expressionPlasmidPeptidesImmunizationAntigenMicrobialCellsViralNucleicCerevisiaeProductionVaccineGenomeTissue cultureBacterialPathogensGeneticGenesVaccinesAntibodyGeneticallyCropsExpressionBiotechnology JournalFungiGeneticsAnalyses of plantFrontiersVectorBiologyHumanSpeciesHigh throughputProcessingResistanceMedicinal plantsPathologyVirusesSignificantly
Proteins26
  • Over the last three decades, the expression of recombinant proteins in plants and plant cells has been promoted as an alternative cost-effective production platform. (frontiersin.org)
  • Although plant systems are now gaining widespread acceptance as a platform for the larger-scale production of recombinant proteins, there is still resistance to commercial uptake. (frontiersin.org)
  • Nevertheless, some plant-derived proteins for research or cosmetic/pharmaceutical applications have reached the market, showing that plants can excel as a competitive production platform in some niche areas. (frontiersin.org)
  • Here, we discuss the strengths of plant expression systems for specific applications, but mainly address the bottlenecks that must be overcome before plants can compete with conventional systems, enabling the future commercial utilization of plants for the production of valuable proteins. (frontiersin.org)
  • The demand for recombinant proteins is therefore rising steadily, with a market valued at US$1.654 billion in 2017 predicted to reach US$2.850 billion by 2022 ( Markets and Markets, 2017 ). (frontiersin.org)
  • Most recombinant proteins are currently produced in prokaryotic cells (mainly the bacterium Escherichia coli ) and a small number of well-characterized mammalian cell lines, such as Chinese hamster ovary (CHO) cells. (frontiersin.org)
  • Even so, plants as an alternative expression platform offer unique advantages, particularly when target proteins are difficult to produce in conventional systems, require specific qualitative properties such as particular glycan profiles, and/or must be produced on a larger scale in response to urgent demand. (frontiersin.org)
  • In 2014, a ebook Recombinant Proteins written by network Joe Sutton was Orwell in America said specially found by the Northern Stage astronomy lack in White River Junction, Vermont. (autumndewilde.com)
  • A technology platform for the fast production of monoclonal recombinant antibodies against plant proteins and peptides. (stanford.edu)
  • The scFvs selected from the library against a diverse set of plant proteins showed moderate to high antigen-binding affinity together with high specificity. (stanford.edu)
  • The following is a list of notable proteins that are produced from recombinant DNA, using biomolecular engineering. (wikipedia.org)
  • In many cases, recombinant human proteins have replaced the original animal-derived version used in medicine. (wikipedia.org)
  • A much larger number of recombinant proteins is used in the research laboratory. (wikipedia.org)
  • As a result, recombinant lysosomal proteins are usually produced in mammalian cells. (wikipedia.org)
  • Recent studies have shown that it may be possible to produce recombinant lysosomal proteins with microorganisms such as Escherichia coli and Saccharomyces cerevisiae. (wikipedia.org)
  • Recombinant lysosomal proteins are used for both research and medical applications, such as enzyme replacement therapy. (wikipedia.org)
  • One of those, recombinant protein producer Bio-Teche, says demand is so high it needs to significantly expand capacity to produce materials like E. coli-derived recombinant proteins. (okchem.com)
  • Thus, striking improvements in recombinant antigen were achieved by alternative polyadenylation signals and fusion proteins containing targeting signals designed to enhance integration or retention of HBsAg in the endoplasmic reticulum (ER) of plant cells [6]. (scielo.br)
  • Proteins of interest (POI) are tagged with a GFP and transiently expressed in plant cells to serve as donor fluorophore. (nature.com)
  • To illustrate our approach, we provide experimental procedures to detect the interaction between plant DNA and two proteins (the AeCRN13 effector from the oomycete Aphanomyces euteiches and the AtWRKY22 defensive transcription factor from Arabidopsis ). (nature.com)
  • Figure 8: GFP lifetime distribution of GFP:AeCRN13 and GFP:AeCRN13 AAA fusion proteins in plant nuclei. (nature.com)
  • Here, we demonstrate that non-infectious SARS-CoV-2 VLPs can be successfully assembled by co-expressing three important viral proteins membrane (M), envelop (E) and nucleocapsid (N) in plants. (nature.com)
  • These advancements will enable continuous production of biotherapeutics such as insulin, monoclonal antibodies, and other recombinant proteins. (who.int)
  • The HMW agents that were considered most relevant were flour dusts, enzymes (both plant and animal derived), gums, foods and tobacco, rubber-derived proteins, animal- and insect-derived allergens, and fish/ seafood-derived allergens ( Table 1 ). (cdc.gov)
  • The main aim of our research is to understand the molecular details and physiological roles of the multitude of water channel-like proteins present in plants and animals. (lu.se)
  • Annotation of Selaginella moellendorffii major intrinsic proteins and the evolution of the protein family in terrestrial plants. (lu.se)
Mammalian4
  • In this study, we demonstrated proofs-of-concept for a technology that uses microfluidics and molecular genomics to capture diverse mammalian antibody repertoires as multivalent recombinant drugs. (biorxiv.org)
  • Further, VLPs have the advantage that, contrary to inactivated or attenuated viruses that must be prepared in mammalian cell lines, they can be produced in heterologous systems, such as bacteria, yeasts, insect cells, or plants. (nature.com)
  • A recombinant-derived analog of beta-glucocerebrosidase produced in mammalian cell culture and chemically modified by mannose termination of glycosylated amino acids. (medscape.com)
  • E. coli, P. pastoris , Mammalian cells, Plant cells and in vitro by cell free synthesis. (lu.se)
Microorganisms1
  • Evolving regulatory policies regarding food enzymes produced by recombinant microorganisms. (msu.edu)
Purification3
  • Market growth has been supported by advances in recombinant protein production technologies, including the engineering of expression hosts, the optimization of upstream cultivation (e.g., bioreactor design, nutritional, and physical parameters), and the development of more efficient protein extraction and purification methods. (frontiersin.org)
  • However, one of the factors that make it difficult and raise the price of production in every system consists in the purification process of the recombinant antigen. (scielo.br)
  • Purification and Proteomic Analysis of Plant Plasma Membranes. (lu.se)
Transgenic7
  • This includes Biosafety Level 1 materials and transgenic animals or plants. (uwm.edu)
  • Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-B fusion protein with a microsomal retention sequence. (elsevierpure.com)
  • Transgenic tobacco plants carrying XDVs absorb various AhR ligands, including 3-methylcholanthrene, β-naphthoflavone and indigo from solid medium and vermiculite, and show dose- and time-dependent expression of the β-glucuronidase reporter gene. (elsevierpure.com)
  • The result showed that, out of 15 putative transformed plants (T0) were analysed, five putative transgenic plant lines were determined to carry r-msAdh1 cDNA in their genome. (unimas.my)
  • The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. (scielo.br)
  • Transgenic lettuce seedlings were obtained through the application of a regulated balance of plant growth regulators. (scielo.br)
  • HBsAg was the first viral antigen chosen to be produced in transgenic plants, firstly in tobacco [3] and subsequently in lupin callus and lettuce adapted to colder climates [4]. (scielo.br)
Antibodies4
  • The application of recombinant antibodies in plant biology research is limited because plant researchers have minimal access to high-quality phage display libraries. (stanford.edu)
  • We are convinced that this antibody platform will further stimulate applications of recombinant antibodies such as the diagnostic detection or immunomodulation of specific antigens in plants. (stanford.edu)
  • These "recombinant hyperimmune" drugs comprised thousands to tens of thousands of antibodies and were derived from convalescent human donors, or vaccinated human donors or immunized mice. (biorxiv.org)
  • During this SARS-CoV-2 pandemic, many studies on the development of plant-derived vaccines and antibodies are underway. (nature.com)
Protein expression1
  • Lund Protein Production Platform (LP3) has recently published its standard operating procedures for recombinant protein expression using the Baculovirus Expression Vector System (BEVS) in insect cells. (lu.se)
Plasmid1
  • Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. (scielo.br)
Peptides2
  • The plants expressed the foreign peptides, both of which formed oligomers that bound the natural ligand. (elsevierpure.com)
  • Phosphatidylinositol synthase activity from plant membrane fractions and from E. coli-expressed recombinant peptides. (illumina.com)
Immunization1
  • Since 1987 two recombinant vaccines produced into Saccharomices cerevisiae cells by Sharp & Dhome and Smith Kline Beechmam have been utilized for HBV immunization. (scielo.br)
Antigen1
  • Here we report a strategy for genetic transformation of lettuce plants (Lactuca sativa L.) using the surface antigen HBsAg of hepatitis B virus. (scielo.br)
Microbial2
  • [16] They can also be classified by their source, such as fungal biotoxins , microbial toxins , plant biotoxins , or animal biotoxins. (wikipedia.org)
  • Microbial Cell Factories invites you to read our collection featuring Microbial synthesis of natural products originating from medicinal plants and fungi . (biomedcentral.com)
Cells4
  • There are also several platforms based on plants and plant cells, but these have not been included in the latest market studies, indicating they have not yet commanded a significant share of commercial protein production capacity. (frontiersin.org)
  • The company estimates that with the number of clinical trials for treatments based on CAR-T cells, hematopoietic and other stem cells, the current global supply of recombinant protein for cell therapy production and large-scale production of treatments will not keep up with demand. (okchem.com)
  • Enzyme replacement therapy (ERT) with glucocerebrosidase purified from human placenta was FDA approved in 1991, followed by approval in 1994 of a recombinant form of the enzyme produced in cultured Chinese hamster ovary (CHO) cells, marketed as imiglucerase (Cerezyme). (medscape.com)
  • This form of recombinant enzyme is produced in cultured human cells and is marketed as velaglucerase alfa (VPRIV). (medscape.com)
Viral1
  • With helpful illustrations, photos, figures, models that explain viral mechanisms, and easy-to-understand reference tables, Plant Viruses As Molecular Pathogens will stimulate your thinking on this fascinating area of plant science! (routledge.com)
Nucleic2
  • ALL recombinant or synthetic nucleic acid molecules or materials that contain these molecules must be decontaminated before disposal. (uwm.edu)
  • Here we describe how to detect nucleic acid-protein interactions in plant leaves by using a fluorescence resonance energy transfer (FRET) approach coupled to fluorescence lifetime imaging microscopy (FLIM). (nature.com)
Cerevisiae2
  • One of the most prominent examples is the engineering of baker's yeast Saccharomyces cerevisiae for the utilization of D-xylose, a five-carbon sugar found in high abundance in lignocellulosic biomass and a key substrate to achieve good process economy in chemical production from renewable and non-edible plant feedstocks. (lu.se)
  • Despite many excellent engineering strategies that have allowed recombinant S. cerevisiae to ferment D-xylose to ethanol at high yields, the consumption rate of D-xylose is still significantly lower than that of. (lu.se)
Production7
  • Furthermore, there are only a few cases in which plants have demonstrated economic advantages compared to established and approved commercial processes, so industry is reluctant to switch to plant-based production. (frontiersin.org)
  • Recombinant human/mouse/rat BMP-2 (355-BM) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line. (novusbio.com)
  • The aim of this project was the development and targeted optimization of recombinant protein production with Bacillus megaterium. (cuvillier.de)
  • Subsequently, recombinant protein production was also assessed on the cellular level - using system wide transcriptome and proteome analysis. (cuvillier.de)
  • The Minneapolis, Minnesota-based company says it will invest between $40 million and $50 million for a new plant specifically for large-scale production. (okchem.com)
  • Plants are being considered as an alternative vaccine/mAb production system, especially in unexpected or emergency situations that cannot be solved by existing systems. (nature.com)
  • LP3 aims to provide a high-quality recombinant protein production service, at as close to cost price as possible. (lu.se)
Vaccine4
  • China's vaccine producer CanSino Biologics announced on Saturday that its recombinant COVID-19 vaccine has been issued a Good Manufacturing Practice (GMP) certificate by Hungarian authorities. (globaltimes.cn)
  • Convidecia, a recombinant vaccine, similar in technology as those two vaccines received no report of blood clots. (globaltimes.cn)
  • CanSinoBIO recombinant vaccine has been approved for conditional marketing in China, and has also obtained emergency use authorization in Mexico, Pakistan, Hungary and Chile. (globaltimes.cn)
  • The vaccine consisted of live recombinant human rabies foci in skunks ( 8,9 ). (cdc.gov)
Genome4
  • Addgene: Expanding the scope of plant genome engineering with Cas12a orthologs and highly multiplexable editing systems. (addgene.org)
  • Motion, G.B., Howden, A.J.M., Huitema, E. & Jones, S. DNA-binding protein prediction using plant specific support vector machines: validation and application of a new genome annotation tool. (nature.com)
  • Genome-wide association study for morphological traits and resistance to Peryonella pinodes in the USDA pea single-plant plus collection. (usda.gov)
  • Genome of 'Charleston Gray', the principal American watermelon cultivar, and genetic characterization of 1,365 accessions in the U.S. National Plant Germplasm System watermelon collection. (msu.edu)
Tissue culture1
  • Use of mutagenesis, tissue culture, plant regeneration. (greenfacts.org)
Bacterial1
  • The recombinant AhRs, referred to as XDVs, consist of the DNA-binding domain of the bacterial repressor protein LexA, a 90-kDa heat shock protein- and ligand-binding regulatory domain from mouse AhR, and the transactivation domain of herpes simplex virus regulatory protein VP16. (elsevierpure.com)
Pathogens1
  • Plant Viruses As Molecular Pathogens is the only book to bring you all of this information--22 chapters--in a single volume, compiled by specialists around the globe! (routledge.com)
Genetic5
  • Plant architecture attributes, such as plant height, ear height, and internode number, have played an important role in the historical increases in grain yield, lodging resistance, and biomass in maize ( Zea mays L . ). Analyzing the genetic basis of variation in plant architecture using high density QTL mapping will be of benefit for the breeding of maize for many traits. (biomedcentral.com)
  • The creation of ultra-high density genetic maps using large populations of advanced recombinant inbred lines (RILs) is an efficient way to identify QTL for complex agronomic traits. (biomedcentral.com)
  • This genetic pattern of 146/Kunming/04/China implied a novel, naturally occurring recombinant NoV with GII/7 capsid and GII/6 polymerase. (cdc.gov)
  • The last decade has seen remarkable advances in plant virological research, owing mainly to the rapid progress made in molecular biology and genetic engineering in recent years. (routledge.com)
  • Genetic characterization of melon accessions in the U.S. National Plant Germplasm System and construction of a melon core collection. (msu.edu)
Genes3
  • Two MYB transcription factor genes, GRMZM2G325907 and GRMZM2G108892, which might regulate plant cell wall metabolism are the candidate genes for qPH10 . (biomedcentral.com)
  • This map will facilitate identification of genes and exploration of QTL for plant architecture in maize. (biomedcentral.com)
  • Discover related pathways, diseases and genes to TRIM50C Recombinant Protein (H00378108-P01). (novusbio.com)
Vaccines1
  • Giving a chance to CanSino's recombinant vaccines, namely Convidecia, to enter more markets globally, according to a notice released by CanSino Biologics. (globaltimes.cn)
Antibody2
  • We also validated a recombinant hyperimmune for Zika virus disease that abrogates antibody-dependent enhancement (ADE) through Fc engineering. (biorxiv.org)
  • Since a plant-derived antibody cocktail to Ebola virus in 2014 had been shown to be effective 5 , more studies and cases have been reported. (nature.com)
Genetically1
  • First field trials of genetically engineered plant varieties in 1990 followed by the first commercial release in 1992. (greenfacts.org)
Crops1
  • Plant architecture directly affects biomass in higher plants, and particularly influences grain yields in agricultural crops. (biomedcentral.com)
Expression3
  • In this study, we have developed novel, dioxin-inducible, reporter gene expression systems regulated by recombinant aryl hydrocarbon receptors (AhRs). (elsevierpure.com)
  • However, expression of either VP1 or both VP1 and VP2 with recombinant baculoviruses formed viruslike particles that are morphologically and antigenically similar to the native virion ( 2 ). (cdc.gov)
  • Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris. (lu.se)
Biotechnology Journal1
Fungi1
  • The medicinal and nutritional value of medicinal plants and fungi are generally represented by the accumulation of bioactive molecules that are in large part constituted by secondary metabolites with anti-cancer, hypoglycemic and antioxidant activities. (biomedcentral.com)
Genetics1
  • Presentation and discussion of a research based topic in the field of plant cellular biochemistry and genetics. (uni-regensburg.de)
Analyses of plant1
  • We are specialised in inorganic analyses of plant, soil, animal and water samples. (lu.se)
Frontiers3
  • Frontiers in Plant Science. (usda.gov)
  • Frontiers Plant Sci. (msu.edu)
  • Frontiers in Plant Science 2012, 3 . (lu.se)
Vector1
  • Department of Health, Wildlife Services, and Ohio local consisting of a live recombinant vaccinia vector, expressing health jurisdictions used print media, television, radio, and rabies virus glycoprotein (V-RG) (Raboral V-RG) ( 2,3 ), was the Internet to raise awareness and provide guidance to the distributed in other areas of Ohio. (cdc.gov)
Biology1
  • BMC Plant Biology 2008, 8 (1):45. (lu.se)
Human2
  • The prefix "rh" for "recombinant human" appears less and less in the literature. (wikipedia.org)
  • Exbumin ® is a recombinant human serum albumin that is manufactured using good manufacturing practices at an ISO9001:2015 certified facility in Kansas, USA. (invitria.com)
Species1
  • Moreover, HPTs in plants must be carried out with both ponderable doses and high dilutions in order for differences in sensitiveness among species be better identified. (researchgate.net)
High throughput2
  • Hybrid spatial-temporal Mueller matrix imaging spectropolarimeter for high throughput plant phenotyping. (usda.gov)
  • Quantitative high-throughput, real-time bioassay for plant pathogen growth in vivo. (msu.edu)
Processing1
  • This partly reflects the relatively low yields achieved in plants, as well as inconsistent product quality and difficulties with larger-scale downstream processing. (frontiersin.org)
Resistance1
  • These three components reflect the spatial conformation of the maize plant, which is closely correlated with biomass, lodging resistance, and tolerance of stress associated with high plant density. (biomedcentral.com)
Medicinal plants1
  • The pdf The Constituents of medicinal plants : an introduction to the chemistry and therapeutics of herbal medicine 2006 return may be in a conscientious Knowledge the imperialism developer Identifying in mbt. (autumndewilde.com)
Pathology1
Viruses2
  • In the presence of DNAβ01, these viruses induced mild leaf curl symptoms in tomato plants. (apsnet.org)
  • While recombinant DNA technology has significantly contributed to our understanding of plant viruses, new findings are being accumulated every day as reported in various publications. (routledge.com)
Significantly1
  • Nexus intends to utilize different plant-based technologies to significantly reduce clinical trial costs, process complexity, quality control and assurance risks. (who.int)