It was found in the 1960s as Pichia pastoris, with its feature of using methanol as a source of carbon and energy. (wikipedia.org)
The previous studies successfully expressed recombinant human insulin precursors (HIP) in Pichia pastoris truncated and full-length α-factor recombinant clones. (springeropen.com)
ImageJ analysis of the HIP's SDS-PAGE shows that the average HIP expression level of the recombinant P. pastoris truncated α-factor clone (CL4) was significantly higher compared to the full-length (HF7) when expressed in both media. (springeropen.com)
Our goal was to develop a general and convenient strategy to improve heterologous protein synthesis in Pichia pastoris without mutating the expressed genes. (biomedcentral.com)
Here, a large-scale deletion library of ribosomal protein (RP) genes was constructed for heterologous protein expression in Pichia pastoris , and 59% (16/27) RP deletants have significantly increased heterologous protein yield. (biomedcentral.com)
Pichia pastoris is a widely used platform for heterologous protein expression, which is a "generally regarded as safe" (GRAS) microorganism. (biomedcentral.com)
Unlike the bacterial expression systems, which lack the modification enzymes, Pichia pastoris is able to produce heterologous proteins with post-translational modifications, especially glycosylation, which is crucial for optimal properties of many pharmaceutical proteins. (biomedcentral.com)
Over 5000 proteins were manufactured in P. pastoris (data from RCT Pichia technology), most of which are industrial enzymes and biopharmaceutical proteins [ 1 , 2 ]. (biomedcentral.com)
However, due to the remarkable codon preference and tRNA content against the other species, heterologous proteins expressed in Pichia pastoris often encounter folding problem, which severely limited their production efficiency [ 3 ]. (biomedcentral.com)
In this study, we used the yeast carotenogenic producer Pichia pastoris Pp-EBIL strain, which has been metabolically engineered, by heterologously expressing β-carotene-pathway enzymes to produce β-carotene, as a. (springeropen.com)
Yeast2
in less formal use, the yeast may confusingly be referred to as pichia. (wikipedia.org)
To date, yeast is the host cell of choice used for the production of recombinant human insulin because of its advantages over other cells [ 4 ]. (springeropen.com)
Proteins1
These properties became critical in the production of recombinant proteins that secrete the protein of interest into the external culture environment. (springeropen.com)
Biopharmaceutical1
Human insulin was the first FDA-approved biopharmaceutical drug produced through recombinant DNA technology. (springeropen.com)