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Mycobacterium smegmatisMycobacteriumMycobacterium bovisMycobacterium tuberculosisVaccines, SyntheticBCG VaccineMycobacterium lepraeBacterial ProteinsMycobacterium aviumMycobacterium InfectionsAntigens, BacterialNontuberculous MycobacteriaTuberculosisRecombinant ProteinsMycobacteriophagesMycobacterium Infections, NontuberculousMycobacterium fortuitumMice, Inbred BALB CMycolic AcidsMycobacterium avium ComplexGenetic VectorsMolecular Sequence DataMycobacterium chelonaeAntitubercular AgentsEthambutolInterferon-gammaMycobacterium phleiMycobacterium marinum

TuberculosisBovisStrainsAntigenNonpathogenicVaccineAvium complexPlasmidBacillusColiProteinInfluxOrganismsProteinsVectorEnzymeGenesGrowthAffinityPulmonaryResistantFunctionalHumanCellCellsMainActivityIncludeExpressionModelsPatients

Tuberculosis11

• Here, we show that the Mycobacterium tuberculosis protease Rv3194c functions not only as an enzyme but as an adhesin. (nih.gov)
• Roach, T.I., Barton, C.H., Chatterjee, D. and Blackwell, J.M. (1993) Macrophage activation: Lipoarabinomannan from avirulent and virulent strains of Mycobacterium tuberculosis differentially induces the early genes c-fos, KC, JE, and tumor necrosis factor-alpha. (scirp.org)
• Immunogenicity of recombinant Mycobacterium smegmatis expressing antigen 85b epitopes as a potential tuberculosis vaccine candidate Nur Ayuni binti Kadir. (unisza.edu.my)
• Mycobacterium tuberculosis (M. tb) is an intracellular pathogen that exploits moonlighting functions of its proteins to interfere with host cell functions. (figshare.com)
• This method has allowed the distinction of Mycobacterium tuberculosis from other mycobacteria and enabled the performance of antimicrobial susceptibility testing of mycobacteria. (medscape.com)
• According to the WHO, one-third of the world's population is infected with Mycobacterium tuberculosis (Mtb) . (biomedcentral.com)
• 2001. Functional Genomics of Mycobacterium tuberculosis Using DNA Microarrays. . (cornell.edu)
• 2001. Recombinant Mycobacterium tuberculosis protein associated with mammalian cell entry. . (cornell.edu)
• 2001. Regulation of the Mycobacterium tuberculosis hypoxic response gene encoding alpha -crystallin. . (cornell.edu)
• Reprogramming of the macrophage transcriptome in response to interferon-gamma and Mycobacterium tuberculosis: signaling roles of nitric oxide synthase-2 and phagocyte oxidase. (cornell.edu)
• Objective · To investigate the bacteriostasis effect of Mycobacterium tuberculosis (MTB) polyphosphate kinase 2 (PPK2) aptamer on MTB in vitro. (shsmu.edu.cn)

Bovis2

• 5. Mycobacterial codon optimization enhances antigen expression and virus-specific immune responses in recombinant Mycobacterium bovis bacille Calmette-Guérin expressing human immunodeficiency virus type 1 Gag. (nih.gov)
• The currently available vaccine, Mycobacterium bovis bacillus Calmette-Guérin (BCG), is one of the oldest and most commonly administered vaccines worldwide [ 4 ]. (biomedcentral.com)

Strains3

• Recombinant BCG strains expressing cytokines have been produced and demonstrated an increased immunotherapeutic potential. (medscape.com)
• The efficacy and safety of recombinant Mycobacterium strains requires testing in clinical trials but may deliver the long-awaited improvement in bladder cancer immunotherapy. (medscape.com)
• Building upon this principle, we have generated quality-controlled, live-recombinant strains of BCG and M. smegmatis which express the immunodominant MU Ag85A. (duke.edu)

Antigen2

• Priming with rBCG MU-Ag85A followed by an M. smegmatis MU-Ag85A boost strongly induced murine antigen-specific CD4+ T cells and elicited functional IFNγ-producing splenocytes which recognized MU-Ag85A peptide and whole M. ulcerans better than a BCG prime-boost vaccination. (duke.edu)
• These results suggest that use of BCG as a recombinant vehicle expressing MU antigens represents an effective Buruli ulcer vaccine strategy and warrants further antigen discovery to improve vaccine efficacy. (duke.edu)

Nonpathogenic2

• 12. Generation of CD8+ T-cell responses by a recombinant nonpathogenic Mycobacterium smegmatis vaccine vector expressing human immunodeficiency virus type 1 Env. (nih.gov)
• [ 107 ] Mycobacterium smegmatis is a nonpathogenic mycobacterium with efficacy comparable with that of BCG in a mouse model. (medscape.com)

Vaccine3

• 2. Development of a Live Recombinant BCG Expressing Human Immunodeficiency Virus Type 1 (HIV-1) Gag Using a pMyong2 Vector System: Potential Use As a Novel HIV-1 Vaccine. (nih.gov)
• 6. Potential of recombinant Mycobacterium paragordonae expressing HIV-1 Gag as a prime vaccine for HIV-1 infection. (nih.gov)
• The obtained results demonstrate that repeated inoculations of BCG effectively activate the humoral immune response, justifying the use of BCG as a live recombinant vaccine vector to insert genes encoding virulence factors controlled by antibodies. (scirp.org)

Avium complex1

• Mycobacterium avium complex (MAC) and Mycobacterium scrofulaceum are associated with lymphadenitis in immunocompetent children. (medscape.com)

Plasmid2

• 3. The development of a novel Mycobacterium-Escherichia coli shuttle vector system using pMyong2, a linear plasmid from Mycobacterium yongonense DSM 45126T. (nih.gov)
• Genes from B. fragilis Bf-2 were cloned on a recombinant plasmid pMT100 which made E. coli AB1157 and uvrA, B, and. (shengsci.com)

Bacillus1

• 18, 19, 20] Affected children show a severe and apparently selective susceptibility to weakly pathogenic mycobacteria (either Bacillus Calmette-Guérin or NTM. (medscape.com)

Coli1

• Conjugation in Escherichia coli by spontaneous zygogenesis and in Mycobacterium smegmatis by distributive conjugal transfer differ from the better studied classical E. coli conjugation in that these cases involve substantial blending of the parental genomes. (wikipedia.org)

Protein1

• Significantly, resuscitation was observed when either recombinant Rpf protein or supernatant derived from a growing bacterial culture was incorporated into the resuscitation medium. (aber.ac.uk)

Influx1

• The growth rate of Mycobacterium smegmatis depends on sufficient porin-mediated influx of nutrients. (nih.gov)

Organisms1

• These studies reveal important differences between cell cycle organization in mycobacteria compared with better-studied model organisms. (nature.com)

Proteins1

• Energy transfer between fluorescent proteins using a co-expression system in Mycobacterium smegmatis. (cornell.edu)

Vector2

• 1. Recombinant Mycobacterium smegmatis with a pMyong2 vector expressing Human Immunodeficiency Virus Type I Gag can induce enhanced virus-specific immune responses. (nih.gov)
• Recombinant Mycobacterium smegmatis (M. smegmatis) containing pe6 displayed robust growth in iron chelated media compared to vector alone transformed cells, which suggests a role of PE6 in iron acquisition. (figshare.com)

Enzyme1

• The binding affinity of the PPK2 aptamer to H37Rv, BCG, Mycobacterium smegmatis, Pseudomonas aeruginosa and Acinetobacter baumannii was analyzed by enzyme-linked oligonucleotide assay (ELONA). (shsmu.edu.cn)

Genes1

• A bioinformatics search on the whole genome sequence of Mycobacterium smegmatis revealed the presence of genes encoding two putative HJRs: ruvC and ruvX. (iisc.ac.in)

Growth4

• smegmatis also showed (transient) loss of culturability following growth to stationary phase in an optimized medium, but under oxygen-limited conditions. (aber.ac.uk)
• smegmatis resume growth when transferred to a suitable resuscitation medium. (aber.ac.uk)
• Nontuberculous mycobacteria (NTM) are classified based on their growth rates. (medscape.com)
• Here we use microfluidics and time-lapse fluorescence microscopy to measure the dynamics of cell growth, division and chromosome replication in single cells of Mycobacterium smegmatis . (nature.com)

Affinity1

• The recombinant MsRuvC was purified to homogeneity using heparin affinity column and gel filtration chromatography under native conditions from the supernatant. (iisc.ac.in)

Pulmonary1

• In the pre-HIV/AIDS era, pulmonary disease and lymphadenitis due to atypical mycobacteria were found all across the United States, with most cases located in the central and southern regions. (medscape.com)

Resistant1

• Mycobacteria have low-permeability outer membranes that render them resistant to most antibiotics. (nih.gov)

Functional1

• Thus, the overall purpose of our research is aimed at a fuller, more complete understanding of the functional aspects of M. smegmatis HJRs RuvC and RuvX. (iisc.ac.in)

Human2

• 11. Generation of mucosal anti-human immunodeficiency virus type 1 T-cell responses by recombinant Mycobacterium smegmatis. (nih.gov)
• This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human MIF. (rndsystems.com)

Cell1

• It is not known how events of the chromosome replication and cell division cycles are coordinated in the distantly related and more slowly growing microorganisms of the genus Mycobacterium . (nature.com)

Cells1

• Conditions were investigated that promote the formation of 'non-culturable' (NC) cells of Mycobacterium (Myc. (aber.ac.uk)

Main2

• An x-ray analysis of the main porin from Mycobacterium smegmatis, MspA, revealed a homooctameric goblet-like conformation with a single central channel. (nih.gov)
• The University of Padova kindly supported the Workshop, offering two wonderful venues: the new main conference room of the "Orto Botanico", heritage of humanity (Botanical Garden, http://www.ortobotanicopd.it/en ), and the main hall of the "Palazzo del Bo" ( https://www.unipd.it/palazzo-bo ) in Padua. (mdpi.com)

Activity1

• The N-terminal domain of OmpATb is required for membrane translocation and pore-forming activity in mycobacteria. (nih.gov)

Include2

• Alternate approaches would include nonbiased determinations by proteomic techniques or spectroscopic approaches such as Raman spectroscopy, which has itself recently been used to identify mycobacteria. (medscape.com)
• The pigmented species are rarely associated in clinical disease and include Mycobacterium phlei, Mycobacterium aurum, Mycobacterium flavescens, Mycobacterium vaccae, Mycobacterium neoaurum, and Mycobacterium thermoresistible. (medscape.com)

Expression1

• In the first half the thesis, we describe the molecular cloning, expression, purification and biochemical characterization of a canonical HJ resolvase RuvC from M. smegmatis (MsRuvC). (iisc.ac.in)

Models1

• Recombinant M. smegmatis expressing TNF-α appeared to be superior to BCG and was nontoxic in immunocompetent, T-deficient or NK-deficient models. (medscape.com)

Patients1

• [ 108 ] Furthermore, it is hoped that M. smegmatis may have minimal adverse side effects and thus be better tolerated by patients. (medscape.com)