A cocktail of five recombinant proteins optimized for conserved GI nematode targets was used immunize pigs and test for active antibody responses in both the serum and intestinal ileal fluid of immunized pigs. (frontiersin.org)
Proteins that activated immunity are potential antigens for immunization and the multi-omics phylum-spanning prioritization database that was created is a valuable resource for identifying target proteins in a wide array of different parasitic nematodes. (frontiersin.org)
Advances in understanding underlying immune mechanisms are feasibale using a variety of candidate antigens, including attenuated live parasites, crude antigens, pure or recombinant Leishmania proteins, Leishmania genes encoding protective proteins, as well as immune system activators from the saliva of parasite vectors. (ac.ir)
however, rabbit antibodies raised against the recombinant proteins encoded by the gene of mouse bullous pemphigoid antigen 2 (BP180) are capable of inducing blisters in newborn mice in a complement-dependent manner. (medscape.com)
Bullous pemphigoid antigen1
Nevertheless, autoantibodies can be induced by immunized healthy BALB/c mice with synthetic peptides of the mouse bullous pemphigoid antigen 2 NC16A domain. (medscape.com)
Capable of inducing1
In addition, rabbit antibodies raised against type VII collagen (epidermolysis bullosa acquisita antigen) are also capable of inducing blisters in mice. (medscape.com)
Foreign antigens1
Classification of Hypersensitivity Reactions Allergic (including atopic) and other hypersensitivity disorders are inappropriate or exaggerated immune reactions to foreign antigens. (msdmanuals.com)
Chimeric3
Chimeric antigen receptors (CAR)-engineered T cells have demonstrated remarkable efficacy in patients with B-cell malignancies. (unimedizin-mainz.de)
If the mouse gene for the whole variable region is spliced next to the human constant region, the product is termed "chimeric. (msdmanuals.com)
Taking advantage of the increased metastatic potential of RhoC-expressing human (A375) melanoma cells, we evaluate four immunodeficient mouse strains: severe combined immunodeficiency (scid), nonobese diabetic (NOD)-scid, NOD-scid beta2m(null), and NOD-scid IL2Rgamma(null) as xenograft tumor recipients. (nih.gov)
Melanoma metastases growth is delayed and variable in scid and NOD-scid mice. (nih.gov)
In contrast, NOD-scid beta2m(null) and NOD-scid IL2Rgamma(null) mice show rapid tumor engraftment, although tumor growth is variable in NOD-scid beta2m(null) mice. (nih.gov)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
In this combined immunodeficiency (SCID) cancer is low in these species (as it chapter, some aspects of this issue mice, in which the human target is in humans), which renders cancer are discussed. (who.int)
Monoclonal antib1
324 In 1973, Jerrold Schwaber described the production of monoclonal antibodies using human-mouse hybrid cells. (wikipedia.org)
Antibody6
Monoclonal antibodies can have monovalent affinity, binding only to the same epitope (the part of an antigen that is recognized by the antibody). (wikipedia.org)
In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect Recombinant Human NAP-2 when used with PeproTech's Biotinylated Antigen Affinity Purified Anti- Human NAP-2 (CXCL7) (500-P03BT) as the detection antibody at a concentration of approximately 1.0-2.0 μg/ml. (peprotech.com)
Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
To minimize the problems due to use of pure mouse antibody, researchers have used recombinant DNA techniques to create monoclonal antibodies that are part human and part mouse. (msdmanuals.com)
In both cases, the process usually begins as above with production of mouse hybridoma cells that make antibody to the desired antigen. (msdmanuals.com)
Then the DNA for some or all of the variable portion of the mouse antibody is merged with DNA for human immunoglobulin. (msdmanuals.com)
Transgenic mouse models2
Here, we evaluated the safety issues raised by the risk of TAA-TCR gene transfer-associated on/off-target toxicities and the therapeutic potential in relevant transgenic mouse models of adoptive transfer. (unimedizin-mainz.de)
We successfully applied this strategy to the human TAAs p53, gp100 and MDM2-specific TCRs as promising antigens-driven immunotherapy for both melanoma and hematologic malignancies. (unimedizin-mainz.de)
Recombinant targeting modules will be based on antibodies and soluble single-chain fragments of TCR specific for melanoma antigens. (unimedizin-mainz.de)
Myeloma cells1
Much of the work behind production of monoclonal antibodies is rooted in the production of hybridomas, which involves identifying antigen-specific plasma/plasmablast cells that produce antibodies specific to an antigen of interest and fusing these cells with myeloma cells. (wikipedia.org)
Protein2
The 148 amino acid recombinant protein (with ADL-His9-SSGIEGR at N-terminus) has a predicted molecular mass of approximately 17 kD. (biolegend.com)
7. Hu C, Gong R, Guo A, Chen H. Protective effect of ligand-binding domain of fibronectin-binding protein on mastitis induced by Staphylococcus aureus in mice. (southernbiotech.com)
Genes4
In adult mice, IL-7/IL-7R signaling up regulates expression of early B cell factor (EBF), and EBF in turn regulates expression of B cell-specific genes required for the transition from lymphoid progenitor to pro-B cells. (biolegend.com)
Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)
Gene2
Using HLA-A2.1 transgenic mice, we have demonstrated the feasibility of T-cell receptor (TCR) gene transfer into T cells to circumvent self-tolerance to the widely expressed human p53 and MDM2 TAAs. (unimedizin-mainz.de)
If the mouse gene for only the antigen-binding hypervariable regions of the variable region is used, the product is termed "humanized. (msdmanuals.com)
Adoptive1
In vivo, DIDS administration delayed B16OVA tumor growth in immunocompetent mice as monotherapy or when combined with adoptive T cell transfer of OVA-specificT cells. (unav.edu)
Immune2
The vaccine-induced immune response is influenced by: (i.) vaccine factors i.e., type and composition of the antigen(s), (ii. (smw.ch)
In this work we intend to investigate this connection through the use of an immunostimulatory odorant like menthol, analyzing its impact on the immune system and the cognitive capacity in healthy and Alzheimer's Disease Mouse Models. (unav.edu)
Rabbit1
Immunoglobulins were detected with serially diluted Mouse Anti-Rabbit IgG-AF555 (SB Cat. (southernbiotech.com)
Contrast1
Counterstains are often applied haphazardly at the end of an IHC procedure with little thought given to optimizing conditions for providing the best contrast with the specific antigen staining. (2bscientific.com)
Viral1
This research project focuses on characterizing the anti-double stranded DNA (dsDNA) response in mice that express the viral Epstein Barr Nuclear Antigen-1 (EBNA-1). (cuny.edu)
Genetic1
To date, the cystoviruses are the only segmented dsRNA viruses that can be manipulated by recombinant genetic techniques, making them an excellent model for the study of the molecular biology of viruses of this type. (cuny.edu)
Immunodeficient3
Immunodeficient mice serve as critical hosts for transplantation of xenogeneic cells for in vivo analysis of various biological processes. (nih.gov)
Because investigators typically select one or two immunodeficient mouse strains as recipients, no comprehensive study has been published documenting differences in human tumor engraftment. (nih.gov)
Menthol inhalation also improved the cognitive capacity of immunocompetent mice but not in immunodeficient NSG mice, which exhibited very poor fear-conditioning. (unav.edu)
Vivo2
The establishment of this in vivo model enabled us to demonstrate that the inoculated mice produced antibodies to autoantigens associated with SLE. (cuny.edu)
For instance, mice are able to reconstitute most lymphomas in monkeys and humans woodchuck hepatitis virus induces major components of the human provides strong support for a direct hepatocellular carcinoma (HCC) haematolymphoid system including oncogenic role of EBV in vivo. (who.int)
Specific6
These abnormal antibodies or paraproteins were used to study the structure of antibodies, but it was not yet possible to produce identical antibodies specific to a given antigen. (wikipedia.org)
In 1975, Georges Köhler and César Milstein succeeded in making fusions of myeloma cell lines with B cells to create hybridomas that could produce antibodies, specific to known antigens and that were immortalized. (wikipedia.org)
However, high-avidity T cells specific for tumor-associated antigens (TAAs) are usually absent in patients because of self-tolerance. (unimedizin-mainz.de)
Hvcn1 overexpression also improved the antitumor activity of CAR T cells specific for Glypican 3 (GPC3) in mice bearing PM299L-GPC3tumors. (unav.edu)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
The stage-specific embryonic antigen 4 (SSEA4) is brain. (lu.se)
Species1
It is a zoonosis, with the primary reservoir species identified as the Natal multimammate mouse, Mastomys natalensis. (folkhalsomyndigheten.se)
Healthy mice2
Blockade of the IL-1 receptor with anakinra resulted in a significant increase in cognitive capacity in healthy mice as well as in the APP/PS1 model of Alzheimer ' s disease. (unav.edu)
So far, no truly active experimental animal models (in which healthy mice are induced to autoimmune disease de novo) are known to facilitate the studies on the induction phase of autoimmune blistering diseases. (medscape.com)
Human5
Produced in mice immunized with highly pure Recombinant Human NAP-2 (CXCL7). (peprotech.com)
When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human NAP-2 is 0.5-1.0 ng/lane, under reducing or non-reducing conditions. (peprotech.com)
In the mouse model, recombinant human SCGB3A2 exhibited anti-fibrotic and growth factor activities comparable to those of recombinant mouse SCGB3A2. (biovendor.com)
Immunohisto- have been used for positive selection of NSCs from em- chemistry on human embryonic central nervous system bryonic mice (Nagato et al. (lu.se)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)
Model3
Exposure to menthol for 6 months (1 week per month) prevented the cognitive impairment observed in the APP/PS1 mouse model of Alzheimer. (unav.edu)
Treg depletion also improved the cognitive capacity of the APP(NL-G-F/NL-G-F) Alzheimer ' s mouse model. (unav.edu)
Model mice for allergic airway inflammation were intranasally administered recombinant adenovirus that express SCGB3A2, and the effect of overexpressed SCGB3A2 on antigen-induced airway inflammation was investigated. (biovendor.com)
Induce1
Passive transfer experiments have demonstrated that purified autoantibodies from patients with the pemphigus group of diseases can induce blister formation when delivered to newborn mice. (medscape.com)
Expression2
The expression of this antigen in animals has been achieved through the use of DNA-based vaccination of recombinant plasmids. (cuny.edu)
Expression of the Epstein-Barr Virus Nuclear Antigen-1 (EBNA-1) in the mouse can elicit the production of anti-dsDNA and anti-Sm antibodies. (cuny.edu)
Cell3
Mouse IL-7 was initially described as a pre B-cell grow factor expressed in bone marrow stromal cells. (biolegend.com)
Natural killer (NK) cell function was examined in each mouse strain using standard (51)Chromium release assays. (nih.gov)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)
Class1
Neefjes J, Jongsma ML, Paul P, Bakke O. Towards a systems understanding of MHC class I and MHC class II antigen presentation. (smw.ch)
Application1
Poor application or selection of a counterstain frequently causes a multitude of issues including color incompatibility, loss of substrate precipitate and obstruction of target antigen staining. (2bscientific.com)