SterileReconstitutionPurityPowderBufferColiBiotinylated Recombinant humanHuman Recombinant ProteinStabilityAntibodyConcentrationELISAGelabelt mit His tagStablePeptideHEK293Biological activitySerially dilutedFormPurificationCarrier proteinVialHEK293TYeastTumorMammalian cellHomodimericLigandsProductCellsAmino acidsBoneSpecificityProductsChimeraExtracellularGrowth-factorMolecular WeightCoomassieReceptor
Sterile7
- It is recommended to reconstitute the lyophilized RBP3 in sterile distilled H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. (prospecbio.com)
- It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). (ptglab.com)
- Lyophilized from sterile PBS, pH 7.4, 5%-8% trehalose and mannitol. (creativebiomart.net)
- Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. (gentaur.com)
- Formulation Sterile filtered, lyophilized from a 1mg/ml solution in 10mM sodium phosphate buffer, pH 8.0 and 50mM NaCl. (qedbio.com)
- Dilution Instructions Reconstitute lyophilized Recombinant Human Prolactin in sterile H2O to not less than 100ug/ml. (qedbio.com)
- BT-ON013 trade name] is a sterile, off-white to pale yellow lyophilized powder for concentrate for solution for infusion. (who.int)
Reconstitution7
- Reconstitution and Dilution: Store lyophilized material at 4°C. Reconstitute in 1 mL of distilled or deionized water. (thermofisher.com)
- For injection: 10 mg of velmanase alfa-tycv as a lyophilized powder in a single-dose vial for reconstitution. (nih.gov)
- Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. (prospecbio.com)
- Lyophilized RBP3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution. (prospecbio.com)
- Store the lyophilized standard at 4°C prior to reconstitution. (bdbiosciences.com)
- Reconstitution of the protein in water to 1 mg/ml leaves the protein in the following buffer: 20 mM Tris pH 7.5, 50 mM NaCl, 0.5% sucrose and 0.1% dextran. (cytoskeleton.com)
- Lyophilized Protein G although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Protein G should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. (abeomics.com)
Purity2
- Purity of recombinant human OSM was determined by SDS- polyacrylamide gel electrophoresis. (ptglab.com)
- Purity is determined by scanning densitometry of protein run on SDS-PAGE gels. (cytoskeleton.com)
Powder5
- Filtered White lyophilized (freeze-dried) powder. (prospecbio.com)
- The recombinant protein is 24 kDa in size and is supplied as a lyophilized powder. (cytoskeleton.com)
- Human ADAP2 Protein Lysate 20ug from Innovative Research is provided as a Lyophilized powder. (innov-research.com)
- This product is provided as lyophilized powder which is shipped with ice packs. (assaygenie.com)
- Aldesleukin is available as lyophilized powder in vials of 22 million IU under the brand name Proleukin. (nih.gov)
Buffer5
- Dilute in a buffer-containing carrier protein, aliquot, and store at or below -70°C, avoiding multiple freeze-thaw cycles. (thermofisher.com)
- CD5L filtered (0.4 µm) and lyophilized from 0.5mg/ml in 50mM Tris buffer, pH 8.6. (prospecbio.com)
- The Human IP-10 Standard should be reconstituted using Assay Diluent, which is provided with the BD™ CBA Human Soluble Protein Master Buffer Kit (Cat. (bdbiosciences.com)
- The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (cusabio.com)
- This protein lysate can be reconsituted using SDS Sample Buffer. (innov-research.com)
Coli9
- CD5L Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 20-347) containing 337 amino acids including a 9 a.a N-terminal His tag. (prospecbio.com)
- RBP3 Human Recombinant (321-630a.a) produced in E.Coli is a single, non-glycosylated Polypeptide chain. (prospecbio.com)
- C3 transferase has been produc ed by expression in E. coli as a His-tagged protein. (cytoskeleton.com)
- Recombinant EGF can be produced using various biological systems such as Escherichia coli , tobacco, rice, yeast, and others. (coherentmarketinsights.com)
- For instance, according to an article published by the American Institute of Physics in June 2020, high levels of recombinant human EGF (rhEGF) were obtained by co-expression of human EGF (hEGF) in Escherichia coli with Bacillus cereus phospholipase C. This rhEGF can then be used for the treatment of diabetic foot ulcers along with other wound treatments. (coherentmarketinsights.com)
- Recombinant Cre recombinase (TAT-Cre) was purified from an E. coli strain carrying an engineered plasmid encoding enhanced form of Cre Recombinase from bacteriophage P1. (excellgen.com)
- Description Human Recombinant Prolactin produced in E.coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 23007 Dalton. (qedbio.com)
- Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antibodies-online.cn)
- Cys-Protein G Recombinant produced in E.Coli, is a single non-glycosylated polypeptide chain containing 201 amino acids and having a cys on N-terminal. (abeomics.com)
Biotinylated Recombinant human2
- Measured by its ability to bind biotinylated Recombinant human/mouse Wnt-5a in a functional ELISA. (rndsystems.com)
- Measured by its ability to compete with Biotinylated Recombinant Human Ephrin‑A5 Fc Chimera (Catalog # BT374 ) for binding to immobilized recombinant mouse Eph-A3 Fc Chimera in a functional ELISA assay. (novusbio.com)
Human Recombinant Protein2
- Full length human recombinant protein of human LTA4H (NP_000886) produced in HEK293T cell. (origene.com)
- Full length human recombinant protein of human LGALS3(NP_002297) produced in HEK293T cell. (origene.com)
Stability2
- Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
- The quality of lyophilized product was measured by thermal stability of the virus, PCR and gene sequencing. (bvsalud.org)
Antibody1
- With years of research and development effort, Fzata has innovated the BioPYM platform, which can produce DNA payloads encoding any kind of protein from small peptides to complicated antibody proteins in the gut. (nature.com)
Concentration5
- It is recommended to add deionized water to a working concentration of 0.5mg/ml and let the lyophilized pellet dissolve completely. (prospecbio.com)
- The concentration of Recombinant Mouse FGF R5 Fc Chimera that produces 50% of the optimal binding is 30-120 ng/mL. (rndsystems.com)
- Protein concentration is determined by the Precision Red Advanced Protein Assay Reagent Cat. (cytoskeleton.com)
- The lyophilized VEGF120 should be reconstituted in ddH 2 O to a concentration not lower than 50 g/ml. (active-bioscience.de)
- Cell survival relative to the control was measured by the XTT method, and plotted against Recombinant human proNGF (cleavage resistant) protein concentration. (alomone.com)
ELISA3
- In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
- Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. (gentaur.com)
- This protein can be used as a positive control for applications such as ELISA, IFA, RIA, Western Blot, etc. (homeodomains.com)
Gelabelt mit His tag1
- Dieses KYNU Protein ist gelabelt mit His tag. (antikoerper-online.de)
Stable3
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. (antikoerper-online.de)
- The lyophilized protein is stable for a few weeks at room temperature. (abcam.com)
- PURPOSE: To construct recombinant lentiviral vectors of bone morphogenetic protein 2(BMP-2) gene and prepare a stable lyophilized state. (bvsalud.org)
Peptide3
- FITC conjugated recombinant human ULBP2 (Q9BZM5) (Met 1-Ser 217),without the pro peptide, w was fused with the Fc region of human IgG1 at the C-terminus. (creativebiomart.net)
- Recombinant Human EGF (Epidermal Growth Factor) is a peptide that binds to the membrane receptor called EGF receptor in humans to promote cell proliferation and cell differentiation. (coherentmarketinsights.com)
- Phospholipase C, immune inhibitor A, chitin-binding protein and a single peptide match to chain A crystal structure of selenomethionine were observed in the secretions of L. infantum promastigotes. (who.int)
HEK2931
- Human IL2RA (P01589, Glu 22 - Cys 213) partial recombinant protein expressed in HEK293 cells. (creativebiomart.net)
Biological activity1
- Activity: The biological activity of recombinant human TNFa was measured by its cytolytic effect on mouse L-929 cells in the presence of Actinomycin D. (thermofisher.com)
Serially diluted2
- 200 ng/mL of biotinylated Recombinant Mouse FGF1 is mixed with serially diluted Recombinant Mouse FGF R5 Fc Chimera. (rndsystems.com)
- The Human IP-10 Standard is lyophilized and should be reconstituted and serially diluted before mixing with CBA capture beads and PE detection reagents. (bdbiosciences.com)
Form6
- Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. (cusabio.com)
- Fig 2: Comparison of recombination acitivities of Tat-Cre recombinase in liquid and lyophilized form. (excellgen.com)
- Form Lyophilized from a 0.2 µm filtered solution. (alomone.com)
- Aldesleukin is a recombinant form of human interleukin-2, a cytokine that stimulates the proliferation and maturation of T cells, which is used in immune therapy of renal cell cancer and malignant melanoma. (nih.gov)
- Aldesleukin (al" des loo' kin) is recombinant form of interleukin-2 (IL-2), a human cytokine produced by macrophages and lymphocytes that induces the proliferation and maturation of T cells. (nih.gov)
- A recombinant form of interleukin-2 has been developed and shown to be an immune modulator and to have antitumor activity against several malignancies, but most convincingly renal cell cancer and malignant melanoma. (nih.gov)
Purification1
- The S. boulardii micro-factories cut out the expensive manufacturing and purification processes typically required for producing protein biologics. (nature.com)
Carrier protein2
- CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)
- C87124 Central Nervous System Signs and Symptoms C96388 Pediatric Immunization Terminology C1455 Conjugate Vaccine Conjugate Vaccine A category of vaccines created by covalently attaching an antigen from an organism to a carrier protein from the same organism to aid in the delivery of the immunogen. (nih.gov)
Vial1
- 1. Open one vial of the lyophilized Human IP-10 Standard. (bdbiosciences.com)
HEK293T2
- HEK293T-cells were transfected with reporter plasmid DNA: LoxP-RFP-Stop-LoxP-GFP, then add 1 μM purified Tat-Cre protein. (excellgen.com)
- This is a Recombinant Protein Lysate produced in HEK293T cells. (innov-research.com)
Yeast8
- The platform leverages yeast as micro-factory to produce disease-targeting protein drugs, enabling the development of more potent biotherapeutics over natural microbiota approaches. (nature.com)
- BioPYMs are packed as dry yeast into capsules that, once swallowed, release the live yeast cells into the intestine, where they secrete the therapeutic proteins encoded by the DNA insert (Fig. 1). (nature.com)
- First, as eukaryotes, yeast cells are closer to human cells and are more likely to produce functional human therapeutic proteins than prokaryotic bacteria. (nature.com)
- A , Orally administered, enteric-coated BioPYM capsule dissolves in the small intestine and releases live, lyophilized recombinant yeast (rYeast) cells. (nature.com)
- The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antibodies-online.cn)
- The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antibodies-online.cn)
- A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. (antibodies-online.cn)
- Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antibodies-online.cn)
Tumor3
- It is known that natural killer (NK) cells take part in innate immunity to remove pathogens and cancer cells, and secrete interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α), macrophage inflammatory protein-1β (MIP-1β) and other molecules to mediate the adaptive immunity. (justia.com)
- The NK cell-secreting cytokines may, in certain embodiments, comprise interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α) or macrophage inflammatory protein-1β (MIP-1β). (justia.com)
- Also known as T cell growth factor, interleukin-2 is a critical cytokine in the adaptive immune process and interacts with specific T cell receptors to activate intracytoplasmic pathways that lead to proliferation and differentiation of immature T lymphoblasts into mature and reactive T cells that play an important role in immune responses to foreign proteins, viruses and bacteria and tumor cells. (nih.gov)
Mammalian cell1
- A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. (antibodies-online.cn)
Homodimeric3
- Recombinant Rat M-CSF is a 36.2 kDa homodimeric protein consisting of two 155 amino acid polypeptide subunits. (abcam.com)
- Recombinant human BMP-7 is a 28.8 kD homodimeric glycoprotein. (biolegend.com)
- The recombinant human ULBP2/Fc is a disulfide-linked homodimeric protein. (creativebiomart.net)
Ligands3
- Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. (novusbio.com)
- This protein can self-associate through the N-terminal domain allowing it to bind to multivalent saccharide ligands. (origene.com)
- Finally, we used immobilized recombinant ER-αto separate ligands for ER and AhR that are present in river sediments. (nih.gov)
Product4
- This product is an active protein and may elicit a biological response in vivo, handle with caution. (abcam.com)
- The following product was used in this experiment: Human TNF-alpha Recombinant Protein from Thermo Fisher Scientific, catalog # RTNFAI. (thermofisher.com)
- This product contains lyophilized recombinant human interferon-γ-induced protein-10 (IP-10). (bdbiosciences.com)
- For long term storage, reconstituted product is best stored at -18°C with the addition of a stabilizing protein such as 0.1% HSA or BSA. (qedbio.com)
Cells9
- B , rYeast cells carry a chromosomal inserted gene encoding therapeutic protein. (nature.com)
- Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. (novusbio.com)
- TF-1 cells were treated with increasing concentrations of GMP recombinant OSM for 96 hours. (ptglab.com)
- Recombinant lyophilized protein for the attachment of human pluripotent stem cells. (bioind.com)
- In further non-limiting embodiments of the method, macrophage inflammatory protein-1β (MIP-1β) can be used as control group for comparing activation of NK cells with that of a normal person. (justia.com)
- Alomone Labs Recombinant human proNGF (cleavage resistant) protein does not promote survival of PC12 cells. (alomone.com)
- Cells were grown in the absence of serum and collagen, and in the presence of varying concentrations of Recombinant human proNGF (cleavage resistant) protein (#N-285). (alomone.com)
- Alomone Labs Recombinant human proNGF (cleavage resistant) protein promotes neurite outgrowth in PC12 cells. (alomone.com)
- Proteomic techniques can show the proteome profile of cells and biological fluids and can also provide more information on protein functions and post-translational modifications of proteins (5). (who.int)
Amino acids2
- For protein verification, the expressed protein was N-terminal sequenced and begins with the following 10 amino acids: VRSSSRTPSD. (thermofisher.com)
- A non-glycosylated protein containing 98 amino acids. (enzolifesciences.com)
Bone1
- Bone morphogenetic proteins (BMPs) belong to the transforming growth factor beta (TGF-β) superfamily. (biolegend.com)
Specificity3
- Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. (cusabio.com)
- RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. (cusabio.com)
- Another immunoblot test, which uses a purified recombinant EpC1 glutathione S-transferase antigen (rEpC1-GST), has a sensitivity of 92.2% and a specificity of 95.6% ( 17 ). (cdc.gov)
Products1
- For instance, in 2018, FUJIFILM, a Japanese multinational conglomerate, acquired Irvine Scientific Sales Company, Inc., a cell media specialist, to expand its range of cell culture media products including recombinant human EGF. (coherentmarketinsights.com)
Chimera1
- Recombinant Mouse FGF R5 Fc Chimera (Catalog # 1899-FR) binds biotinylated Recombinant Mouse FGF1. (rndsystems.com)
Extracellular4
- Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. (creativebiomart.net)
- Cartilage oligomeric matrix protein (COMP), also designated thrombospondin 5 (TSP 5), is a non-collagenous glycoprotein and is a member of the thrombospondin family of extracellular proteins. (biovendor.com)
- This protein localizes to the extracellular matrix, the cytoplasm and the nucleus. (origene.com)
- Fn14 expression decreases cellular adhesion to extracellular matrix proteins fibronectin and vitronectin and reduces cell growth and migration. (enzolifesciences.com)
Growth-factor4
- Connective Tissue Growth Factor (CTGF) is a member of cysteine rich regulatory proteins that are both mitogenic and chemotactive. (enzolifesciences.com)
- The fibroblast growth factor-inducible-14 (Fn14) is a 129 aa type Ia transmembrane protein. (enzolifesciences.com)
- Fn14 is a growth-factor regulated, immediate-early response protein. (enzolifesciences.com)
- A related protein of VEGF is placenta growth factor (PlGF) with about 53% homology and VEGF-B with similar biological activities. (active-bioscience.de)
Molecular Weight1
- Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
Coomassie2
- The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue. (ptglab.com)
- A 20 µg sample of CT03 was separated by SDS-PAGE and protein was stained with coomassie blue. (cytoskeleton.com)
Receptor2
- This protein can also interact with receptor TNFRSF6/FAS and adaptor protein FADD/MORT1, and is involved in the Fas-induced cell death pathway. (avivasysbio.com)
- ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. (assaygenie.com)