OrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation Science
LipasePichiaLipoprotein LipaseRecombinant ProteinsMolecular Sequence DataMonoacylglycerol LipasesAmino Acid SequenceCloning, MolecularRecombinant Fusion ProteinsSterol EsteraseBase SequenceDNA, RecombinantMethanolColipasesGenetic Vectors
PastorisProteinsMethylotrophic yeastBile-salt sCarboxyl esterColipase-dependentEnzymesPutativePancreaticSaccharomycesGeneTriglyceridesPolypeptideInsect cellsAspergillusBaculovirusProtein productionMoleculeProductionYeastCandidaBacterialAcidCatalyticEfficientlyMethodsActivityHighCommercialApplicationFound
Pastoris17
  • The potential for enzyme therapy was tested using mannose terminated human LAL expressed in Pichia pastoris (phLAL), purified, and administered by tail vein injections to lal( -/-) mice. (nih.gov)
  • 3) Although each of these lipases can be isolated from pancreatic secretions or from the gland, most recent structure-function studies have utilized recombinant proteins purified from either baculovirus or Pichia pastoris expression systems. (pancreapedia.org)
  • This chapter will describe methods for the production and purification of pancreatic lipases in those systems with emphasis on the P. pastoris system. (pancreapedia.org)
  • Yeast expression systems meet these requirements and virtually all labs that produce recombinant lipases utilize yeast, generally the methylotropic yeast Pichia pastoris . (pancreapedia.org)
  • During growth on methanol and medium with no added proteins, P. pastoris secretes low levels of endogenous proteins and the recombinant protein comprises the majority of protein in the medium facilitating subsequent purification. (pancreapedia.org)
  • 2009 ) or Pichia pastoris (Han et al. (springeropen.com)
  • His research career has been focused on Biochemical Engineering, especially in the fields of Bioprocess Engineering, Production of Recombinant Proteins in the cell factory Pichia pastoris, monitoring modelling and control of bioprocesses and Applied Biocatalysis (lipases). (ibisba.eu)
  • In the present study, a lipase-encoding gene, TllipA , was cloned from Trichoderma lentiforme ACCC30425 and expressed in Pichia pastoris GS115. (springeropen.com)
  • Expression in Pichia pastoris was explored as an alternative route for high level production of the D-PhgAT. (biomedcentral.com)
  • Abdelmoula-Souissi S., Zouari N., Miladi-Abdenadher I., Yaich-Kolsi O., Ayadi-Masmoudi I., Khabir A., Masmoudi H., Frikha M., Mokdad-Gargouri R. (2013) Secreted recombinant P53 protein from Pichia pastoris is a useful antigen for detection of serum p53: autoantibody in patients with advanced colorectal adenocarcinoma. (cbs.rnrt.tn)
  • In this context, native methylotrophs such as the yeast Komagataella phaffii (syn Pichia pastoris ) are potentially attractive cell factories to produce a wide range of products from this highly reduced substrate. (biomedcentral.com)
  • Komagataella phaffii (also known as Pichia pastoris ) is a methylotrophic yeast strain capable of assimilating methanol as a sole source of carbon and energy. (biomedcentral.com)
  • Methylotrophic yeast such as Pichia pastoris are commonly used for expression of recombinant proteins. (justia.com)
  • This disclosure describes the use of P. pastoris strains that overexpress the transcriptional activator, Mxr1, from the AOX1 promoter to increase expression of transgenes that also are expressed from the AOX1 promoter, which significantly improves the recombinant production of one or more proteins. (justia.com)
  • In some embodiments, the exogenous nucleic acid encoding a transcriptional activator comprises a Mxr1 sequence from Pichia pastoris , a Adr1 sequence from Hansenula polymorpha , a Trm1 sequence from Candida boidinii , and a Trm2 sequence from Candida boidinii . (justia.com)
  • In this context, the suitability of methylotrophic yeast Pichia pastoris for recombinant protein production has stand out over the classical yeast Saccharomyces cerevisiae, presenting an increasing relevance for the production of commercial recombinant products. (uab.cat)
  • Finally, it was performed a comparative transcriptomic analysis of the response of P. pastoris to the expression of three different recombinant proteins, to study the impact of protein complexity and the use of constitutive or inducible expression systems. (uab.cat)
Proteins5
  • PTL is the archetype of a lipase gene family that includes PTL homologues, pancreatic lipase-related proteins 1 and 2 (PLRP1 and PLRP2). (pancreapedia.org)
  • 13, 18, 19) The system has several advantages for expression of recombinant proteins. (pancreapedia.org)
  • For structure-function studies of lipases, an expression system that is easily and quickly manipulated, that processes and secretes lipases correctly and that can produce milligram or larger quantities of proteins is preferable. (pancreapedia.org)
  • Recombinant mammalian proteins readily undergo correct folding, proteolytic processing, disulfide bond formation and glycosylation. (pancreapedia.org)
  • Contribution to a better food system with the production of recombinant animal proteins via model-based optimization and bioreactor cultivation. (ibisba.eu)
Methylotrophic yeast3
  • In one aspect, a methylotrophic yeast cell is provided that includes a recombinant nucleic acid molecule. (justia.com)
  • Representative methylotrophic yeast can be of the genus Candida, Hansenula, Pichia or Toruplosis . (justia.com)
  • In some embodiments, the recombinant nucleic acid molecule is stably integrated into the genome of the methylotrophic yeast cell. (justia.com)
Bile-salt s4
  • Bile salt-stimulated lipase (or BSSL) is an equivalent enzyme found within breast milk. (wikipedia.org)
  • A prospective, randomized, double-blind crossover study comparing rhBSSL (recombinant human Bile Salt Stimulated Lipase) and placebo added to infant formula during one week of treatment in preterm infants born before 32 weeks of gestational age. (wikipedia.org)
  • Human milk bile-salt stimulated lipase. (wikipedia.org)
  • Also present is carboxyl ester lipase (CEL), alternatively known as bile-salt stimulated lipase (BSSL). (pancreapedia.org)
Carboxyl ester1
  • Bile salt-dependent lipase (or BSDL), also known as carboxyl ester lipase (or CEL) is an enzyme produced by the adult pancreas and aids in the digestion of fats. (wikipedia.org)
Colipase-dependent2
Enzymes5
  • Lipase (EC 3.1.1.3) is regarded as one of the most important commercial enzymes, and has been attracting enormous attention in the rapidly growing biotechnological area. (springeropen.com)
  • Au sein de celle-ci, les actions combinées de trois enzymes, lipase, lipoxygénase (LOX) et hydroperoxyde lyase (HPL), convertissent un substrat lipidique (acides C 18:2 et C 18:3 ) en molécules volatiles à courte chaine. (ac.be)
  • Combined actions of three enzymes: lipase, lipoxygenase (LOX) and hydroperoxide lyase (HPL) convert lipidic substrates such as C 18:2 and C 18:3 fatty acids into short chain volatiles. (ac.be)
  • Recombinant protein production is becoming the preferred option to synthesized biopharmaceutical and industrial enzymes. (uab.cat)
  • The potential of antimicrobial peptides, clay minerals, egg yolk antibodies, essential oils, eucalyptus oil-medium chain fatty acids, rare earth elements and recombinant enzymes are discussed. (biomedcentral.com)
Putative1
  • Whole-genome sequencing of the adapted strains reveals that inactivation of LPL1 (encoding a putative lipase) and IZH3 (encoding a membrane protein related to zinc metabolism) preserve cell survival by restoring phospholipid metabolism. (nature.com)
Pancreatic2
  • The first attempts to produce large quantities of pancreatic lipases for structure-function studies utilized baculovirus expression systems. (pancreapedia.org)
  • The original descriptions of pancreatic lipase production in the baculovirus system used the PVL1392 transfer vector, linearized genomic DNA from Autographa californica nuclear polyhedrosis virus (AcMNPV) and insect cells from Spodoptera frugoperda (Sf9). (pancreapedia.org)
Saccharomyces6
  • Extraction and purification of hepatitis B virus-like M particles from a recombinant Saccharomyces cerevisiae strain using alumina powder. (cbs.rnrt.tn)
  • Description: Saccharomyces Cerevisiae TIM14, recombinant protein. (ksiazkiwnauce.pl)
  • Description: Saccharomyces Cerevisiae Heat Shock Protein 104, recombinant protein. (ksiazkiwnauce.pl)
  • Description: Saccharomyces Cerevisiae Cytosine Deaminase, recombinant protein. (ksiazkiwnauce.pl)
  • Description: Saccharomyces Cerevisiae Activator of Heat Shock 90kDa Protein ATPase Homolog 1, recombinant protein. (ksiazkiwnauce.pl)
  • Description: HSV type 2 gG antigen fused with human superoxide dismutase 1 (SOD1), recombinant protein from Saccharomyces cerevisiae. (ksiazkiwnauce.pl)
Gene1
  • To further increase the recombinant protein production, several strategies have been attempted, being the increase of the heterologous gene dosage one of the most widely reported. (uab.cat)
Triglycerides2
  • Lysosomal acid lipase (LAL) is the critical enzyme for the hydrolysis of the triglycerides (TG) and cholesteryl esters (CE) delivered to lysosomes. (nih.gov)
  • In adipose tissue, hormone-sensitive lipase (HSL) decomposes triglycerides to produce glycerol and fatty acids. (assaygenie.com)
Polypeptide2
  • Rhizomucor miehei lipase (RML) is an α / β -type single-chain polypeptide composed of 269 amino acids with a Ser-Asp-His catalytic triad structure (Boel et al. (springeropen.com)
  • Through molecular biology techniques, the pentapeptide sequence, molecular weight, and architecture of the ELP can be precisely controlled for subsequent an purified as a recombinant polypeptide, resulting in monodisperse, non-immunogenic (Urry et al. (justia.com)
Insect cells1
  • 21) In combination with insect cells, baculovirus can produce high levels of recombinant lipases with appropriate post-translational modifications. (pancreapedia.org)
Aspergillus2
  • In this study, the effect of biosurfactant sophorolipids (SLs) on Rhizomucor miehei lipase (RML) fermentation by Aspergillus oryzae was investigated. (springeropen.com)
  • For example, the lipase from Aspergillus niger having a narrow and curved channel shows relatively low activity, while those from Ophiostoma piceae , Nectria haematococca and Trichoderma reesei have straightforward and wider channels and much higher activities (Barriuso et al. (springeropen.com)
Baculovirus1
  • Since the original descriptions of lipase expression in baculovirus systems, multiple improvements have been made in baculovirus expression systems. (pancreapedia.org)
Protein production2
  • Consequently, the yeast can be grown to high density in glycerol-containing medium without any recombinant protein production. (pancreapedia.org)
  • Once the appropriate cell density is reached recombinant protein production is induced by switching to methanol-containing medium. (pancreapedia.org)
Molecule2
  • The recombinant nucleic acid molecule typically includes an exogenous nucleic acid encoding a transcriptional activator operably linked to at least one methanol-inducible promoter element. (justia.com)
  • In some embodiments, the recombinant nucleic acid molecule is extrachromosomally expressed from a replication-competent plasmid. (justia.com)
Production3
  • However, the lipase production capacity of wild type R. miehei is relatively low, and the composition of the enzyme solution is unstable. (springeropen.com)
  • Par conséquent, des systèmes de production compétitifs ont été développés afin de subvenir à la haute demande en molécules aromatiques naturelles. (ac.be)
  • Bouzid M., Gargouri A., Belghith H. (2013) Application of a Chitosan-Immobilized Talaromyces thermophilus Lipase to a Batch Biodiesel Production from Waste Frying Oils. (cbs.rnrt.tn)
Yeast1
  • Most vectors contain one of several different yeast signal sequences to target the recombinant protein to the secretory pathway. (pancreapedia.org)
Candida1
  • For example, the lipases from Candida rugosa with different hydrophobic zones at the central channel showed different activities (Mancheño et al. (springeropen.com)
Bacterial1
  • Nevertheless, bacterial and fungal lipases are of special interest as they are easily produced and favorable for industrial purposes due to the high yields and great versatility and stability under harsh conditions (Schmid 2016 ). (springeropen.com)
Acid1
  • According to the protein structure similarity, lipase belongs to the family of α/β hydrolases, in which a catalytic triad (usually serine, histidine, and aspartic or glutamic acid) and an oxyanion hole (just like a catalytic pocket) are crucial for catalysis (Gupta et al. (springeropen.com)
Catalytic2
  • SlCLH1/2/3/4 contained a highly conserved GHSXG lipase motif and a Ser-Asp-His catalytic triad. (inra.fr)
  • We identified Ser159, Asp226, and His258 as the essential catalytic triad by site-directed mutagenesis in recombinant SlCLH1. (inra.fr)
Efficiently1
  • 2004 ). Since the 1960s, enzyme-based detergent has been introduced into the market, and lipase that efficiently removes acylglycerols has been one of the major additives in cleaning agent (Abo 1990 ). (springeropen.com)
Methods2
  • Finally, the most common assay methods for triglyceride lipases will be described. (pancreapedia.org)
  • 21) Transfer vectors have been improved as have methods for purification of recombinant virus. (pancreapedia.org)
Activity1
  • In addition, the lipase activity is also related to the size and orientation of the channel. (springeropen.com)
High1
  • and 3 lipase not only has high biodegradation ability but also brings harmless effect on aquatic ecosystems (Jurado et al. (springeropen.com)
Commercial1
  • In comparison to commercial lipases, Tl LipA demonstrated higher tolerance to various surfactants (SDS, Tween 20, and Triton X100) and retained more activities after incubation with Triton X100 for up to 24 h. (springeropen.com)
Application1
  • Alkaline lipases with adaptability to low temperatures and strong surfactant tolerance are favorable for application in the detergent industry. (springeropen.com)
Found1
  • The purified recombinant Tl LipA was found to have optimal activities at 50 °C and pH 9.5 and retain stable over the pH range of 6.0-10.0 and 40 °C and below. (springeropen.com)