Recombinant gene expression. Medical search. Frequent questions

Expression vectors are the basic tools in biotechnology for the production of proteins. (wikipedia.org)
Other fusion proteins such as green fluorescent protein may act as a reporter gene for the identification of successful cloned genes, or they may be used to study protein expression in cellular imaging. (wikipedia.org)
The expression host of choice for the expression of many proteins is Escherichia coli as the production of heterologous protein in E. coli is relatively simple and convenient, as well as being rapid and cheap. (wikipedia.org)
The insect cell-baculovirus expression vector system (IC-BEVS) has emerged as a mainstream platform for the scalable production of recombinant proteins with clinically approved products for human use. (mcgill.ca)
Gene expression in living cells is strictly self-regulated to ensure that the correct amounts of proteins are made at the most appropriate timing and location for maintaining cellular homeostasis. (nature.com)
The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)
The latter are composed of key genes from the 1918 virus, updated by subsequently incorporated avian influenza genes that code for novel surface proteins, making the 1918 virus indeed the 'mother' of all pandemics. (cdc.gov)
Recombinant proteins were expressed, and antisera against these proteins were produced in rabbits. (avma.org)
The Thermo Scientific 1-Step Human High-Yield In Vitro Translation (IVT) Kits enable the expression of functional proteins with 10 to 100 times greater yield per mL than other mammalian IVT systems. (thermofisher.com)
In this system, protein expression is performed in a proprietary dialysis device that allows a continuous supply of nucleotides, amino acids and energy generating substrates into the reaction while removing inhibitors of proteins synthesis. (thermofisher.com)
The advantages of using the 1-Step Human High-Yield IVT Kits over traditional in vivo expression systems include the ability to express toxic or insoluble proteins, easily perform protein labeling with modified amino-acids and reduce the time and cost of expressing human proteins in tissue culture cells. (thermofisher.com)
Of the four proteins invested in some detail (green fluorescent protein (GFP), red fluorescent protein (RFP), Neu5Ac synthetase NeuB, Neu5Ac synthetase SiaC), 3 showed identical specific activities when expressed from the re-coded gene as when expressed from an ACA-free gene, and one (RFP) showed a slightly reduced specific activity. (europa.eu)
Thus, striking improvements in recombinant antigen were achieved by alternative polyadenylation signals and fusion proteins containing targeting signals designed to enhance integration or retention of HBsAg in the endoplasmic reticulum (ER) of plant cells [6]. (scielo.br)
The eukaryotic model organism Saccharomyces cerevisiae is potentially a good expression host as it tolerates several stress conditions and is known to better express insoluble proteins compared to bacterial systems. (lu.se)
They have found wide use in studies of genetics, toxicity screening, nutrition and gene expression, particularly to express recombinant proteins. (labroots.com)
Biologics, including monoclonal antibodies, recombinant proteins, and vaccines, are in high demand owing to their remarkable specificity and effectiveness. (reportsanddata.com)
The production of biologics relies significantly on expression vectors, which serve as vehicles to introduce foreign genes into host cells for the expression of recombinant proteins. (reportsanddata.com)
Both the production of recombinant proteins and the development of in vitro diagnostic assays for drug research heavily rely on the utilization of expression vectors. (reportsanddata.com)
Plasmid vectors are commonly used in gene therapy, vaccine development, and the production of recombinant proteins. (reportsanddata.com)
Several features of lentiviral vectors have made them advantageous for the expression of therapeutic proteins in vivo. (jci.org)
The drug substance anakinra is a recombinant protein (Interleukin-1 receptor antagonist) produced in E. coli bacteria containing an expression plasmid in which a synthetic gene coding for human IL-1Ra has been inserted.According to the Guideline on the Environmental Risk Assessment of Medicinal Products for Human Use (EMEA 2006), proteins are exempted from environmental risk assessment because they are unlikely to result in significant risk to the environment. (janusinfo.se)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)

This review summarizes the characteristics of various transcriptional and posttranscriptional regulatory elements, and highlights their influence on the expression performance of rAAV vectors in the mammalian brain. (nih.gov)
There are differences in the machinery for protein synthesis between prokaryotes and eukaryotes, therefore the expression vectors must have the elements for expression that are appropriate for the chosen host. (wikipedia.org)
For example, prokaryotes expression vectors would have a Shine-Dalgarno sequence at its translation initiation site for the binding of ribosomes, while eukaryotes expression vectors would contain the Kozak consensus sequence. (wikipedia.org)
Gene expression however may also be constitutive (i.e. protein is constantly expressed) in some expression vectors. (wikipedia.org)
Low level of constitutive protein synthesis may occur even in expression vectors with tightly controlled promoters. (wikipedia.org)
A second approach to achieve neuronal type-specific expression is to use a cell type-specific promoter, and we have used the tyrosine hydroxylase (TH) promoter to restrict expression to catecholaminergic neurons or a modified neurofilament heavy gene promoter to restrict expression to neurons, and both of these promoters support long-term expression from HSV-1 vectors. (harvard.edu)
To both improve nigrostriatal-neuron specific expression, and to establish that targeted gene transfer can be followed by long-term expression, we performed targeted gene transfer with vectors that support long-term, neuronal-specific expression. (harvard.edu)
Results: Helper virus-free HSV-1 vector packaging was performed using either gC - GDNF or gC - BDNF and vectors that contain either the TH promoter or the modified neurofilament heavy gene promoter. (harvard.edu)
The need for effective and scalable expression systems for protein synthesis in research and development is driving demand for expression vectors. (reportsanddata.com)
New York, 27 Oct, 2023 - The global expression vectors market size was USD 2.26 Billion in 2022 and is expected to register a revenue CAGR of 6.3% over the forecast period, according to the latest report by Reports and Data. (reportsanddata.com)
Furthermore, advances in gene therapy and genetic engineering technologies are driving demand for expression vectors. (reportsanddata.com)
To transmit therapeutic genes for disease therapy or prevention, gene expression vectors must be efficient and secure. (reportsanddata.com)
Similarly, when applying genetic engineering techniques such as CRISPR/Cas9 gene editing, accurate and trustworthy expression vectors are required for targeted gene change. (reportsanddata.com)
This involves the development of customized expression vectors for targeted gene expression in certain patient groups. (reportsanddata.com)
Furthermore, the growth of Research and Development (R&D) endeavors in the pharmaceutical and biotechnology domains fuels the demand for expression vectors. (reportsanddata.com)
The Food and Drug Administration (FDA) in the United States is in charge of regulating gene therapy products, including expression vectors used in clinical trials. (reportsanddata.com)
The European Commission has implemented regulations for gene therapy goods, including expression vectors, as well as for the safety of participants in gene therapy clinical studies. (reportsanddata.com)
In 2022, plasmid vectors held a substantial revenue share of the market for expression vectors. (reportsanddata.com)
The increased research activity in a range of disciplines, including as drug discovery, proteomics, genomics, and synthetic biology, is increasing the need for expression vectors in research applications. (reportsanddata.com)
Furthermore, rising funding for research projects by governmental and commercial organizations is expected to boost demand for expression vectors in the research application sector. (reportsanddata.com)
In 2022, North America had the highest revenue share in the Expression Vectors Market, followed by Europe and Asia Pacific. (reportsanddata.com)
In addition, the area has well-established regulatory frameworks and supportive policies that assist the development and marketing of sophisticated biotechnology goods such as expression vectors. (reportsanddata.com)
Furthermore, the expanding trend of precision medicine and gene treatments is predicted to fuel demand for improved expression vectors, adding to regional market revenue growth. (reportsanddata.com)
Some of the prominent players profiled in the global expression vectors market include Thermo Fisher Scientific Inc, Promega Corporation, Takara Bio Inc, Agilent Technologies Inc, Merck KGaA, QIAGEN N.V, Lonza Group AG, GenScript Biotech Corporation, New England Biolabs Inc, and Clontech Laboratories Inc. (reportsanddata.com)
Verheust C, Goossens M, Pauwels K, Breyer D. Biosafety aspects of modified vaccinia virus Ankara (MVA)-based vectors used for gene therapy or vaccination. (medscape.com)
Carroll MW, Moss B. Poxviruses as expression vectors. (medscape.com)

Efficiency and stability of recombinant adeno-associated virus (rAAV)-mediated gene expression within the mammalian brain are determined by several factors. (nih.gov)
We have evaluated the antitumor effect of HSVtk/ganciclovir gene therapy under Tet-On regulation by means of adeno-associated virus-2 (AAV-2)-mediated HSVtk gene transfer with direct intratumoral injections in mice bearing breast cancer tumors. (biomedcentral.com)
Recombinant adeno-associated virus-2 (rAAV) was constructed and transduced into MCF-7 cell line. (biomedcentral.com)

In the first phase of the project cell lines of specific leukocyte subsets (THP-1/monocyte, IM-9/T lymphocyte, H9/B lymphocyte), PBMCs (peripheral blood mononuclear cells) from healthy donors, respectively will be treated in vitro with hgh to identify candidate genes influenced by hghapplication. (wada-ama.org)
To find additional hgh-candidate genes, suppression subtractive hybridization (SSH) technology will be applied on hgh-treated PBMCs and on those cultured leukocyte subsets which showed a response to hgh before during in vitro studies. (wada-ama.org)
To this purpose cell lines of specific leukocyte subsets (THP-1/ monocyte, IM-9/ B- lymphocyte, H9/ T-lymphocyte) and PBMCs (peripheral blood mononuclear cells) from healthy donors, respectively were treated in vitro with hgh to identify candidate hgh-sensitive genes. (wada-ama.org)
Thus, using a rat contusion spinal cord injury model we examined the efficacy of the construct on tissue sparing, glial scar severity, the extent of axonal regeneration, recovery of motor function, and analyzed the expression of the recombinant genes VEGF and GNDF in vitro and in vivo" comments one of the authors Yana Mukhamedshina. (sciencedaily.com)
1-Step Human IVT Kits are optimized for use with the pT7CFE1 expression vector that utilizes the T7 viral promoter and an EMCV Internal Ribosome Entry Site (IRES) to facilitate high levels of in vitro protein expression. (thermofisher.com)

An expression vector, otherwise known as an expression construct, is usually a plasmid or virus designed for gene expression in cells. (wikipedia.org)
Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. (scielo.br)

Better understanding of new mutations and the wide range of possible phenotypes led to the development of a new nomenclature proposal, based on the gene and inheritance pattern. (medscape.com)
[ 6 ] However, the gene mutations responsible for the different forms of CMT1 are clearly myelin genes. (medscape.com)
To investigate the biological role of these RSV G gene mutations, recombinant RSV strains harboring either a wild type A2 strain G gene (one stop codon preceding a wild type gene end signal), an A4G gene end signal preceded by one stop codon, or the 2stop+A4G virulence-associated combination were generated and characterized. (cdc.gov)
Zettner MA, Flor S, Ludewig G, Wagner J, Robertson LW, Lehmann L. Quinoid metabolites of 4-monochlorobiphenyl induce gene mutations in cultured Chinese hamster v79 cells. (who.int)

Rimmelzwaan GF, Sutter G. Candidate influenza vaccines based on recombinant modified vaccinia virus Ankara. (medscape.com)

An expression vector has features that any vector may have, such as an origin of replication, a selectable marker, and a suitable site for the insertion of a gene like the multiple cloning site. (wikipedia.org)
The cloned gene may be transferred from a specialized cloning vector to an expression vector, although it is possible to clone directly into an expression vector. (wikipedia.org)
The objective of work presented by Sanjay M. Reddy of Texas A&M University was to evaluate potential cloning sites in the HVT genome for stable expression of foreign genes. (thepoultrysite.com)
Cloning and characterization of a serine/threonine protein phosphatase2A-encoding gene IbPP2A1 from Ipomoea batatas (L.) Lam. (tubitak.gov.tr)

Reverse genetics: NS1 genes will be altered through site mutagenesis and mutated viruses will be generated through co-transfection of macropahges with a reverse genetics system to determine which part of the NS1 genes is responsible for the observed differences in cytopathogenic effect (apoptosis) and the modulation of the immnue genes detected by microarray analysis. (usda.gov)

The present project aims to introduce microarray technology as a new analytical tool into the field of doping control analysis to gain insight in specific effects of recombinant human growth hormone (hgh) on blood cells (leukocytes). (wada-ama.org)
Microarray technology will be used to search for changes in leukocyte gene expression which are directly related to the application of human growth hormone (hgh). (wada-ama.org)
After confirming SSH-genes to be differentially expressed in a series of microarray experiments, oligonucleotide probes will be designed for both SSH-genes and the candidate genes identified along gene expression profiling using whole genome/leukocyte relevant arrays. (wada-ama.org)
To establish a selective "hgh-chip" the newly designed oligonucleotide probes for the hghcandidate genes will be printed on a microarray. (wada-ama.org)
Cultures will be grown in triplicate and realtime RT-PCR will be used on individual cultures to confirm the microarray findings on selected genes, which will initially include: MHC class I, MHC class II, MHC B-G, IL-1b, IL-4, and CCL5. (usda.gov)
The Affymetrix software Microarray Suite 4.0 and 5.0 (MAS 4 and MAS 5) estimate expression from the average difference of PM and MM fluorescence. (biomedcentral.com)
The microarray analysis included 715 genes and was conducted in triplicate. (cdc.gov)

The vector is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. (wikipedia.org)
The promoter initiates the transcription and is therefore the point of control for the expression of the cloned gene. (wikipedia.org)
Cell-free gene expression systems have been valuable tools for understanding how transcription/translation can be regulated in living cells. (nature.com)
Gene regulation can occur at any point in gene expression, from the start of the transcription phase to the translation phase. (nature.com)
To elucidate the complex mechanism of gene regulation in living cellular systems, it is necessary to closely investigate each phase of gene expression and also to shed light on the cooperative effects between transcription and translation. (nature.com)
The ribosome assembly gene network is controlled by the feedback regulation of transcription elongation. (us.es)
β-catenin then translocates to the nucleus where it forms a complex with TCF/LEF transcription factors and activates Wnt target gene expression 28 , 29 . (nature.com)
The complete maxi-scale kit includes all the components required for transcription and translation of a recombinant gene, including an optimized expression vector. (thermofisher.com)
The 1-Step Human High-Yield IVT Kits are cell-free protein expression systems that provide all of the essential components required for transcription and translation. (thermofisher.com)
The expression of HSVtk gene in MCF-7 cells and BALB/C nude mice tumors was up-regulated by Tet-On under Dox induction with reverse transcription-PCR (RT-PCR) analysis. (biomedcentral.com)
The A4G component is a prevalent point mutation in the 4(th) position of the gene end transcription termination signal of the G gene of currently circulating RSV strains. (cdc.gov)
The 2stop component is two tandem stop codons at the G gene terminus, preceding the gene end transcription termination signal. (cdc.gov)

Human IgG antibody Laboratories manufactures the recombinant antibodies rabbit reagents distributed by Genprice. (genprofiling.com)
Data on expression of VP2, as measured by antibody levels and protection against challenge, in commercial chickens was presented. (thepoultrysite.com)
Expression of INF-γ, INF-β, IL-1β, IL-2, IL-6, IL-18 in the bursa of Fabricius using a multiplex assay, percentage of bursal weight to body weight (BW: BW), bursal histopathology, and antibody titres against IBDV were compared following oral administration of 'variant' IBDV in groups of birds at day-3 or day-6 post-hatch. (thepoultrysite.com)
The STAR platform contains genetic elements, called STAR elements, that enable stable and high-yield gene expression for recombinant antibody and protein production in mammalian cells, Crucell explains. (genengnews.com)

We also found that interferon (IFN) and IFN-inducible genes were regulated differentially in A/ph/CA/2373/98 (H9N2) virus infected Macrophages. (usda.gov)
Clustering analysis based on expression profiles and gene function revealed sets of genes differentially regulated between proliferating and differentiating C2CI2 cells. (cdc.gov)

Identification of this gene and expression of recombinant cytotoxin could facilitate the development of improved vaccines against infectious bovine keratoconjunctivitis. (avma.org)
Since 1987 two recombinant vaccines produced into Saccharomices cerevisiae cells by Sharp & Dhome and Smith Kline Beechmam have been utilized for HBV immunization. (scielo.br)

The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. (scielo.br)

A capture IgM EIA (non-quantitative) that incorporates a recombinant measles virus nucleocapsid protein as the antigen is used to detect measles IgM. (cdc.gov)
However, one of the factors that make it difficult and raise the price of production in every system consists in the purification process of the recombinant antigen. (scielo.br)

Whole genome cDNA microarrays as well as oligonucleotide microarrays including leukocyte-relevant genes will be used to characterize potential hgh-specific genes by comparing gene expression profiles of treated and untreated cells. (wada-ama.org)
Results obtained from gene expression profiling utilising whole genome arrays were in accordance with published data. (wada-ama.org)
Further analysis also showed that r-msAdh1 cDNA have passed into all T1 transgenic lines and expression analysis at transcript level confirmed the presence of r-msAdh1 in transgenic tomato genome. (unimas.my)
For this purpose, the VP2 gene of infectious bursal disease virus (IBDV) was cloned into CVRM genome by recombination. (thepoultrysite.com)
Influenza A virus (IAV), a member of the Orthomyxoviridae family, possesses a negative strand RNA genome made up of eight gene segments. (biomedcentral.com)
The gradual conversion from endosymbiont to organelle during the course of evolution has clearly been accompanied by a dramatic reduction in genome size as the chloroplasts lost most of their genes to the nucleus. (frontiersin.org)

Genes for non-apoptotic cell death and regulation of osmotic pressure were down-regulated. (wada-ama.org)
Due to the complexity of gene regulation, unveiling the complete mechanism of gene regulation has been a long-standing quest. (nature.com)
The aforementioned complex factors have made it difficult to investigate the impact of the DNA length on gene regulation, which has been an unexplored topic. (nature.com)
However, only a few workers have reported precise regulation of the HSVtk gene. (biomedcentral.com)

We have constructed a recombinant adenovirus, Ad-hUGT1A1-CTLA4Ig that coexpresses human bilirubin-uridinediphosphoglucuronate glucuronosyltransferase (hUGT1A1) and soluble murine CTLA4Ig, both driven by CMV immediate-early promoters. (nih.gov)
In this manuscript, we have developed a recombinant human adenovirus as an expression vector that contains the gene for porcine interferon alpha. (usda.gov)
In contrast, control animals inoculated with an adenovirus lacking the interferon gene developed typical signs of FMD. (usda.gov)

The objective of the work presented in Chicago was to evaluate CVMR-BAC as an expression vector of foreign genes. (thepoultrysite.com)

While recombinant DNA technology has significantly contributed to our understanding of plant viruses, new findings are being accumulated every day as reported in various publications. (routledge.com)
The recombinant HVT-VP2 viruses were stable and showed continuous expression of VP2 even after several passages in cell culture. (thepoultrysite.com)
These four viruses have been selected because they have NS1 genes that belong to the A and B alleles, respectively. (usda.gov)
We anticipate that NS1 mediates the effects of AIV on macrophages and would like to test viruses with different NS1 alleles to determine if it mediates the downregulation of immune function genes as we described in the Prelimiary Results. (usda.gov)

The E. coli rhamnose-inducible promoter (Prha) is virtually silent in the absence of rhamnose, and served well as the regulatory structure for the MazF gene. (europa.eu)
To reduce potentially toxic effect on the host cell, the growth and production phases were separated by using the inducible promoter GAL1p to control recombinant gene expression. (lu.se)

The promoters used in expression vector are normally inducible, meaning that protein synthesis is only initiated when required by the introduction of an inducer such as IPTG. (wikipedia.org)
Expression of IFN-inducible protein IP-30 was suppressed up to 2.4 fold but that of interferon-inducible 58 kDa with tetratricopeptide repeats 5 (IFIT-5) was increased up to 20.1 fold in the early stage of infection (6 hrs post infection). (usda.gov)

A Matter of Packaging: In uence of Nucleosome Positioning on Heterologous Gene Expression. (us.es)

En: Methods in Molecular Biology. (us.es)
Consequently, there is a pressing need for efficient and reliable methods of expression to facilitate the large-scale production of these biologics. (reportsanddata.com)
We have tested heritability-weighted averages to define expression of a transcript and found that these allow detection of more QTLs than previously described methods. (biomedcentral.com)
Several alternative methods have been proposed to combine multiple probe-specific values into a single expression estimate. (biomedcentral.com)
The course presents methods to express genes, which have products that are known, unknown, or not well characterised, and to analyse the gene products. (lu.se)

Oligonucleotide primers, designed on the basis of amino acid sequences of 2 tryptic peptides derived from 1 such protein and conserved regions of the C and B genes from members of the repeats in the structural toxin (RTX) family of bacterial toxins, were used to amplify cytotoxin-specific genes from M bovis genomic DNA. (avma.org)

Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis. (us.es)

Furthermore, we co-administered intraperitoneally recombinant murine interleukin-2 (rIL-2) with the rL H5 to enhance HSI. (biomedcentral.com)
The MHC gene region of murine hosts influences the differential tissue tropism of infecting Trypanosoma cruzi strains. (fiocruz.br)

The combination of targeted gene transfer with neuronal-specific promoters improved nigrostriatal neuron-specific expression (83 to 93%) compared to either approach alone, and supported long-term (1 month) expression at levels similar to those observed using untargeted gene transfer. (harvard.edu)
Conclusion: Targeted gene transfer can be used in combination with neuronal-specific promoters to achieve a high level of nigrostriatal neuron-specific expression. (harvard.edu)
The progress was made in increasing the product of therapeutic genes combined with suicide gene therapy using either tumor cell-specific or tissue-specific promoters. (biomedcentral.com)
The PEP enzyme recognizes the -10 and -35 cis -elements, similar to those found in bacterial promoters whereas the NEP enzyme recognizes the YRTA-motif, which can also be found upstream of several genes with PEP promoters indicating that these genes can be transcribed by both polymerases ( Pfannschmidt and Liere, 2005 ). (frontiersin.org)

GDNF reduces apoptosis and tissue degeneration, supports expression of neurofilament protein, calcitonin gene-related peptide (CGRP) and growth associated protein 43. (sciencedaily.com)
TNF-alpha induced increased expression of genes coding mediators of apoptosis, cell-matrix interactions, proteolysis and C-C chemokines. (cdc.gov)

Injected lentiviral vector preferentially transduced splenic dendritic cells and resulted in long-term expression. (jci.org)

The recombinant protein named FoxTPI was purified (97% purity) showing a molecular mass of 27 kDa, with optimal activity at pH 8.0 and and temperature of 37 °C. The values obtained for Km and Vmax using the substrate GAP were 0.47 ± 0.1 mM, and 5331 µmol min-1 mg-1, respectively. (bvsalud.org)

Host immune responses limit the duration of expression of transgenes introduced by recombinant adenoviruses, preclude gene transfer upon vector readministration and cause liver injury. (nih.gov)

1. Extensive Regulations of immune genes in chicken macrophages and lungs infected with influenza virus H9N2. (usda.gov)

Next, 12 ACA- free genes, originating from 4 different microorganisms, were assembled into three different operons, encoding the entire 12-step pathway. (europa.eu)

From Texas A&M University, Blanca Lupiani reported that she and her colleagues recently showed that insertion of LTR (long term repeat) sequences into the CVI988 resulted in the generation of CVRM, a virus with similar protective efficacy.The CVRM virus was cloned as a bacterial artificial chromosome, by insertion of mini-F sequences into the US2 gene by homologous recombination. (thepoultrysite.com)

Background: Direct gene transfer into neurons has potential for developing gene therapy treatments for specific neurological conditions, and for elucidating neuronal physiology. (harvard.edu)
HSV-1 vector particles containing either gC - GDNF or gC - BDNF target gene transfer to nigrostriatal neurons, which contain specific receptors for GDNF or BDNF. (harvard.edu)
Immunofluorescent costaining was performed to detect both recombinant gene products and nigrostriatal neurons. (harvard.edu)

To make this purification process easier, a purification tag may be added to the cloned gene. (wikipedia.org)

Baculovirus has emerged recently as a novel and attractive gene delivery vehicle for mammalian cells. (unboundmedicine.com)
The resultant recombinant baculovirus (BV-G-HA) mediated gene delivery and HA expression efficiently in mammalian cells. (unboundmedicine.com)

The prin2.2 and csp41b-2 single mutants displayed pale phenotypes, abnormal chloroplasts with reduced transcript levels of photosynthesis genes and defects in embryo development. (frontiersin.org)

Results of cytotoxin neutralization assays support the hypothesis that M bovis cytotoxin is encoded by this gene and belongs in the RTX family of bacterial exoproteins. (avma.org)

Genes of groups similar to those detected as repressed in H9 cells were also down-regulated in the RA-1 cell line along with genes responsible for muscle development. (wada-ama.org)
Since the first report of baculovirus-induced production of rAAV vector in insect cells in 2002, this platform has undergone significant improvements, including enhanced stability of Bac-vector expression and a reduced number of baculovirus-coinfections. (mcgill.ca)
Here, we aimed at identifying a β-catenin-independent Wnt target gene signature in order to understand the functional significance of non-canonical signaling in colon cancer cells. (nature.com)
Independent experiments confirmed several target genes, including PLOD2 , HADH , LCOR and REEP1 as non-canonical target genes in various colon cancer cells. (nature.com)
For this study, researchers of Kazan Federal University and Kazan State Medical University chose human umbilical cord blood mononuclear cells (UCB-MCs), easy to produce and safe, with low immunogenicity and the potential to increase neuroregeneration, transduced with these two genes VEGF and GDNF. (sciencedaily.com)
CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics. (labroots.com)
The cells were cultured in media to allow for proliferation for 24 hrs followed by differentiation media for 2, 24 and 48 hrs in the presence or absence of recombinant mouse TNF-alpha. (cdc.gov)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)

In silico analysis of available array data showed that a large number of genes traditionally classified as PEP dependent genes are transcribed during early embryo development from the pre-globular stage to the mature-green-stage. (frontiersin.org)

Gene expression profiling was performed after silencing of key components of Wnt signaling pathway and an iterative signature algorithm was applied to predict pathway-dependent gene signatures. (nature.com)

Considering the action of VEGF and GDNF through different receptors and pathways, we hypothesized that the simultaneous delivery of these two therapeutic genes would promote synergistic neuroprotective effects. (sciencedaily.com)

The antitumor effect of rAAV-mediated HSVtk/GCV gene therapy under the Dox induction with direct intratumoral injections may be a useful treatment for breast cancer and other solid tumors. (biomedcentral.com)

A respiratory syncytial virus attachment (G) gene variant associated with more severe disease in infants decreases fusion (F) protein expression which may facilitate immune evasion. (cdc.gov)

In this study, mice were vaccinated with recombinant virus vaccine (rL H5), in which the hemagglutinin (HA) gene of influenza A/H5N1 virus was inserted into the LaSota Newcastle disease virus (NDV) vaccine strain. (biomedcentral.com)
Female mice lacking this gene do not form a stable zona matrix and are sterile. (nih.gov)

The expression of IdeS was followed by constructing a fused protein with GFP and measuring the fluorescence with flow cytometry. (lu.se)

The expression level from luc 25.7k is 1000-times higher than that with luc 1.7k, indicating that the protein yield per target gene is increased by 1000-times of magnitude for a longer template, luc 25.7k (Fig. 1 d). (nature.com)
One approach is to target gene transfer to a specific type of neuron. (harvard.edu)

It is expected that the enhancement of gene expression efficiency with a shrunken DNA conformation would also be a rather general mechanism in living cellular environments. (nature.com)
Due to the complex cellular composition of specific brain areas, neuronal type-specific recombinant gene expression is required for many potential applications of neuronal gene transfer. (harvard.edu)

The "hgh-chip" will then be thoroughly evaluated by performing numerous gene expression experiments on hgh-treated and untreated leukocyte subsets, cultured PBMCs as well as PBMCs obtained from hgh-patients. (wada-ama.org)

The expression of selected genes was validated by real time RT -PCR, for both the direction and magnitude of the gene expression observed by the cDNA array. (cdc.gov)

Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
the gene expression profile during B lymphoid differentiation. (lu.se)

It is our expectation that this recombinant vector can provide protection against IBD and highly virulent MD challenge, concluded Dr Lupiani. (thepoultrysite.com)
Swine given one inoculation of this recombinant virus and then challenged with virulent FMDV one day postinoculation were completely protected from clinical signs of disease and viremia. (usda.gov)

As such, in order to achieve complete therapeutic action, the therapeutic gene must be delivered not only to the epicenter of traumatic injury but also to the surrounding areas distant from the epicenter of injury. (sciencedaily.com)

Berry intake changes hepatic gene expression and DNA methylation patterns associated with high-fat diet. (lu.se)
tures and gene expression patterns. (lu.se)

The goal of a well-designed expression vector is the efficient production of protein, and this may be achieved by the production of significant amount of stable messenger RNA, which can then be translated into protein. (wikipedia.org)

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Facilitate1

Mice2

Fluorescence1

Target gene2

Cellular2

Experiments1

CDNA1

Differentiation2

Virulent2

Therapeutic gene1

Patterns2

Stable1