Recombinant dna clones. Medical search. Frequent questions

To engineer organisms or produce valuable proteins, scientists create recombinant DNA constructs by combining DNA from different sources. (biotecnika.org)
Over the next 100 years, many significant discoveries lead to the conclusions that genes encode proteins and reside on chromosomes, which are composed of DNA. (addgene.org)
These findings culminated in the central dogma of molecular biology, that proteins are translated from RNA, which is transcribed from DNA. (addgene.org)
The giver DNA is separated from molecular components of the cell such as proteins, RNA, lipoids and saccharides. (free-essays.us)
In transduction, the recombinant molecule is combined with phage caput and tail proteins from one cell to another utilizing bacteriophage vector. (free-essays.us)
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. (sigmaaldrich.com)
Thus, striking improvements in recombinant antigen were achieved by alternative polyadenylation signals and fusion proteins containing targeting signals designed to enhance integration or retention of HBsAg in the endoplasmic reticulum (ER) of plant cells [6]. (scielo.br)
A nanoscale modular design strategy was employed to synthesize six engineered, recombinant proteins intended to mimic aspects of the extracellular matrix proteins fibronectin, laminin, and elastin as well as the cell-cell adhesive protein neural cell adhesion molecule. (frontiersin.org)
The proteins are synthesized using recombinant, genetic engineering techniques, allowing for the creation of biocompatible polymers with nanoscale precision that impart highly specific protein functionalities. (frontiersin.org)
We make use of a highly flexible multi-step cloning strategy in order to allow for the rapid synthesis of new recombinant proteins that can introduce many different biofunctionalities. (frontiersin.org)
understand the theoretical basis for laboratory work (cloning, recombinant expression of proteins, protein purification, etc. (tum.de)

What are Cloning Vectors and Recombinant DNA Constructs? (biotecnika.org)
Scientists harness the power of cloning vectors and recombinant DNA constructs to engineer organisms, develop life-saving drugs, and study the fundamental mechanisms of life. (biotecnika.org)
This article explores the essential building blocks of biotech: cloning vectors and recombinant DNA constructs, shedding light on how they are used to unlock the secrets of DNA. (biotecnika.org)
This article delves into two essential components of biotech: cloning vectors and recombinant DNA constructs. (biotecnika.org)
Before we delve into cloning vectors and recombinant DNA, let's refresh our knowledge of DNA. (biotecnika.org)
Cloning vectors are the workhorses of genetic engineering. (biotecnika.org)
Two common types of cloning vectors are plasmids and bacteriophages. (biotecnika.org)
These fragments are then inserted into cloning vectors , such as bacterial plasmids or bacteriophages, which transfer the recombinant DNA to suitable host cells, such as the bacterium E. coli. (encyclopedia.com)
Alternatively, complementary DNA is inserted into the vectors, or 'naked' DNA fragments can be taken up directly by a host bacterium from its medium (this is less efficient than vector transfer). (encyclopedia.com)
Our study indicates that co-expression of GP and VP40 antigens based on the SFV replicon vector generates EBOV VLPs in vitro , and vaccination with recombinant DREP vectors containing GP and VP40 antigens induces Ebola antigen-specific humoral and cellular immune responses in mice. (frontiersin.org)
Plasmids are widely used as vectors to produce recombinant DNA for gene cloning. (synbiosis.com)
Any DNA molecule that has the ability to replicate inside the host to which the desired gene is integrated for cloning- include plasmids, bacteriophages, cosmids, BAC, yeast vectors, shuttle vectors etc. (biologyexams4u.com)
Cloning vectors are used for obtaining millions of copies of the cloned DNA fragments. (biologyexams4u.com)
The cloned genes in these vectors are not expected to express themselves at the transcription or translation level. (biologyexams4u.com)
Cloning vectors (Cloning vehicles) are used for creating genomic libraries, preparing probes, genetic engineering, or other basic studies. (biologyexams4u.com)
The most important requirement for recombinant DNA technology is the cloning vector and expression vectors. (biologyexams4u.com)
Cosmids are the novel cloning vectors which possess properties of both plasmid and phage. (biologyexams4u.com)
Cosmid can be defined as the hybrid vectors derived from plasmids that contain cos site of phage which is essential for packaging of nucleic acid into protein coat plus essential features of plasmids and several unique restriction sites for the insertion of DNA to be cloned. (biologyexams4u.com)
Because of the importance of DNA in the replication of new structures and characteristics of living organisms, it has widespread importance in recapitulating via viral or non-viral vectors, both desirable and undesirable characteristics of a species to achieve characteristic change or to counteract effects caused by genetic or imposed disorders that have effects upon cellular or organismal processes. (wikidoc.org)
[1] In the classical instance, the use of recombinant DNA provides the initial cell from which the host organism is then expected to recapitulate when it undergoes further cell division, with bacteria remaining a prime example due to the use of viral vectors in medicine that contain recombinant DNA inserted into a structure known as a plasmid . (wikidoc.org)
The use of plasmids is also key within gene therapy , where their related viruses are used as cloning vectors or carriers, which are means of transporting and passing on genes in recombinant DNA through viral reproduction throughout an organism. (wikidoc.org)

Plasmids are circular pieces of DNA found in bacteria. (biotecnika.org)
Plasmids are fragments of double-stranded DNA that typically carry genes and can replicate independently from chromosomal DNA. (addgene.org)
Plasmids that are used most commonly in the field of recombinant DNA technology have been optimized for their use of studying and manipulating genes. (addgene.org)
Typically plasmids contain the minimum essential DNA sequences for this purpose, which includes a DNA replication origin, an antibiotic-resistance gene, and a region in which exogenous DNA fragments can be inserted. (addgene.org)
This led us to construct recombinant DNA plasmids containing the encoding sequence of HBsAg and the same construction fused with the SEQDEL sequence responsible for ER sorting. (scielo.br)
They published their findings in a 1974 paper entitled "Construction of Biologically Functional Bacterial Plasmids in vitro ", which described a technique to isolate and amplify genes or DNA segments and insert them into another cell with precision, creating a transgenic bacterium . (wikidoc.org)
Plasmids are extrachromosomal self-replicating circular forms of DNA present in most bacteria, such as Escherichia coli (E. Coli), containing genes related to catabolism and metabolic activity, [1] and allowing the carrier bacterium to survive and reproduce in conditions present within other species and environments. (wikidoc.org)
[1] Plasmids contain three common features -- a replicator , selectable marker and a cloning site . (wikidoc.org)

Like the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division, and each daughter cell receives at least one copy of the plasmid. (addgene.org)
By the 1970s the combined discoveries of restriction enzymes, DNA ligase, and gel electrophoresis allowed for the ability to move specific fragments of DNA from one context to another, such as from a chromosome to a plasmid. (addgene.org)
The combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule, which can be used to study DNA fragments of interest, such as genes. (addgene.org)
The plasmid Acts of the Apostless as a cloning vector, provides reproduction of cloned cistron inside the host cell. (free-essays.us)
The PureYield Plasmid Maxiprep System isolates transfection-quality plasmid DNA, providing a rapid method to purify up to 1mg of plasmid DNA from a 250ml bacterial culture. (promega.com)
Purify plasmid DNA rapidly with the vacuum protocol, greatly reducing time spent compared to silica resin or other membrane-column methods. (promega.com)
This system incorporates a unique Endotoxin Removal Wash to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. (promega.com)
The PureYield Plasmid Midiprep System isolates transfection-quality plasmid DNA, providing a rapid method to purify 100-200ug of plasmid DNA from a 50ml bacteria culture. (promega.com)
The PureYield Plasmid Miniprep System is designed to rapidly isolate highly pure plasmid DNA. (promega.com)
The system provides a rapid method for purification of up to 15ug of plasmid DNA from 600ul to 3ml of bacteria culture. (promega.com)
Plasmid DNA can be purified in as little as 10 minutes. (promega.com)
A plasmid is made up of DNA that can exist ad replicate independently of the chromosome. (synbiosis.com)
Genetic transformation process was acquired by cocultivation of cotyledons with Agrobacterium tumefaciens harboring the recombinant plasmid. (scielo.br)
First artificial cloning vector (1977) constructed by Boliver & Rodriguez from E .coli plasmid. (biologyexams4u.com)
[1] In terms of genetic modification , recombinant DNA is produced through the addition of relevant DNA into an existing organismal genome , such as the plasmid of bacteria, to code for or alter different traits for a specific purpose, such as immunity. (wikidoc.org)
In the introduction of useful genes into the plasmid, the bacteria are then used as a viral vector, which are encouraged to reproduce so as to recapitulate the altered DNA within other cells it infects, and increase the amount of cells with the recombinant DNA present within them. (wikidoc.org)
The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)

They are small, self-replicating pieces of DNA that scientists use to carry and replicate genes of interest. (biotecnika.org)
Molecular Biology introduces you to the structure and function of molecules, including DNA and RNA, which allow genes to be expressed and be maintained from one generation to the next. (manchester.ac.uk)
[2] Through the use of recombinant DNA, genes that are identified as important can be amplified and isolated for use in other species or applications, where there may be some form of genetic illness or discrepancy, and provides a different approach to complex biological problem solving. (wikidoc.org)
While the regions affected in recombinant chromosome 8 includes hundreds of genes, researchers are working to determine which genes play a role in the signs and symptoms of this condition. (medlineplus.gov)

Topics covered include cell structure and function, DNA replication and the flow of genetic information, enzyme function, metabolism and physiology of microorganisms, plants, and animals. (sfu.ca)
DNA replication: semi-conservative replication, DNA polymerases, events at the replication form, replication of the lagging strand, telomeres, consequences of defects in telomerase. (manchester.ac.uk)
Replication of genomes: origins of replication, control of DNA replication. (manchester.ac.uk)
Replication of double stranded DNA. (edu.gh)
Compatible with multiplexed RT-PCR and NGS-based assays, AccuPlex custom recombinant virus materials are constructed with a replication-defective mammalian virus, producing a safe, non-infectious material (Figure 1). (seracare.com)
1990. Cell replication and unscheduled DNA synthesis (UDS) activity of low molecular weight chlorinated paraffins in the rat liver in vivo. (cdc.gov)

The following product was used in this experiment: TERT Recombinant Rabbit Monoclonal Antibody (JF0586) from Thermo Fisher Scientific, catalog # MA5-42464, RRID AB_2911605. (thermofisher.com)
To minimize the problems due to use of pure mouse antibody, researchers have used recombinant DNA techniques to create monoclonal antibodies that are part human and part mouse. (msdmanuals.com)
Then the DNA for some or all of the variable portion of the mouse antibody is merged with DNA for human immunoglobulin. (msdmanuals.com)
The resultant DNA is placed in a mammalian cell culture, which then expresses the resultant gene, producing the desired antibody. (msdmanuals.com)

The need for multidisciplinary perspectives, along with advances in genomics, recombinant DNA, and the digital revolution, has changed how scientists communicate and collaborate -- laying bare the interdependence of biology, the physical sciences, and mathematics. (carleton.edu)

The obtainment of transgenic edible plants carrying recombinant antigens is a desired issue in search for economic alternatives viewing vaccine production. (scielo.br)

The two DNA molecule sites can crossbreed to each other through their reconciliation gluey ends to organize a recombinant molecule. (free-essays.us)
In transmutation, recombinant DNA molecule enters into bacteriums cells. (free-essays.us)
in a mouse model, a molecularly engineered FIX molecule maintained protection against bleeding approximately three times longer, compared with recombinant FIX. (medscape.com)

Techniques in chemistry enable isolation and purification of cellular components, such as DNA, but practically this isolation is only feasible for relatively short DNA molecules. (addgene.org)
The recombinant molecules do non hold covalent bond on rear sugar medieties, the DNA ligase used to seal the sugar molecules and anchor which creates phosphodiester bonds at the functions.DNA ligase widely used in lab and it is derived from the bactriophage T4. (free-essays.us)
Lec 2 - The 3 Rs of DNA: Molecules to Medicine" (September 25, 2009) Gilbert Chu, Stanford School of Medicine Professor of Medicine and Biochemistry, discusses how DNA works and recombination works, and how these great discoveries are both advantageous and problematic for medicinal treatment. (dnatube.com)

Two independently isolated recombinant phage were isolated from the library and were found to possess DNA necessary for expression of type b capsule. (jci.org)

Deoxyribonucleic acid, or DNA, contains the genetic instructions that make each living organism unique. (biotecnika.org)
They must besides hold convenient limitation sites at which the Deoxyribonucleic acid to be cloned may be inserted. (free-essays.us)
DNA Deoxyribonucleic acid. (cdc.gov)

Scholars@Duke publication: Molecular cloning of the gene sequences of a major apoprotein in avian very low density lipoproteins. (duke.edu)
Recombinant DNA is a form of artificial DNA that is engineered through the combination or insertion of one or more DNA strands, thereby combining DNA sequences that would not normally occur together. (wikidoc.org)
The Committee concluded that full-length genome sequences from additional variola major and minor strains, particularly Congo 70 and Somalia 77, should be determined and that additional clone libraries from selected strains should be prepared. (who.int)
We happen to have very high-quality DNA sequences for this extinct species. (medscape.com)

First, a full-length functional ZIKV cDNA clone was engineered as a bacterial artificial chromosome, with each reporter gene under the cap-independent translational control of a cardiovirus-derived internal ribosome entry site inserted downstream of the single open reading frame of the viral genome. (mdpi.com)
In this study, we constructed a recombinant Alphavirus Semliki Forest virus (SFV) replicon vector DREP to express EBOV GP and matrix viral protein (VP40). (frontiersin.org)

Since 1987 two recombinant vaccines produced into Saccharomices cerevisiae cells by Sharp & Dhome and Smith Kline Beechmam have been utilized for HBV immunization. (scielo.br)
In 1987 the sdhCAB operon of B. subtilis was cloned and sequenced. (lu.se)

The Recombinant DNA technology and genetic engineering. (wikipedia.org)
9) The student analyzes the importance of recombinant DNA technology and genetic engineering. (tx.us)

The American Society of Human Genetics offers a DNA Day Essay Contest, a High School Workshop at its annual meeting, and career resources. (vault.com)
Lecture: developing chemical fundaments of genetics (DNA, RNA), bacterial genetics, gene expression and regulation, pecularities of eukaryotic organisms. (tum.de)

We would be happy to know if anyone knows certain general tricks (appropriate E. coli host, growth temperature, DNA preparation, etc) that is useful when working with such DNA? (bio.net)
A vector used to clone DNA fragments of 100-300 kb insert size in E.coli cells. (biologyexams4u.com)

In order to isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence of a few hundred or few thousand basepairs from the entire human genome. (addgene.org)
Digesting the human genome with restriction enzymes would yield about two million DNA fragments, which is far too many to separate from each other for the purposes of isolating one specific DNA sequence. (addgene.org)
Employing the DNA blot analysis only one COMT-encoding gene was found in the rat genome. (lu.se)

Cloning of prokaryotic and eukaryotic genomes. (edu.gh)

What is a cloning vector? (biologyexams4u.com)
YAC (Yeast Artificial Chromosome): A vector of hundreds to kilobases long used for cloning of DNA fragment. (biologyexams4u.com)
It is a 4362 kb long widely used cloning vector. (biologyexams4u.com)

human insulin, for example, is now produced by bacteria containing the cloned insulin gene. (encyclopedia.com)
One of the biggest mileposts, readying of HUMAN INSULIN utilizing recombinant DNA engineering. (free-essays.us)

In the world of biotechnology, the ability to manipulate DNA is at the heart of countless groundbreaking discoveries and innovations. (biotecnika.org)
At its core, biotechnology relies on our understanding of DNA and the tools we use to manipulate it. (biotecnika.org)
My favorite class so far has been Biotechnology (BIT) 410 Manipulation of Recombinant DNA because it was my first taste of what it's like to create a recombinant organism. (ncsu.edu)
This core biotechnology class delves into the basics of moving a gene from one organism to another through the process of molecular cloning and transformation. (ncsu.edu)
I fell in love with BIT 410 Manipulation of Recombinant DNA because of the range of applicability of biotechnology. (ncsu.edu)

The DNA containing the target gene(s) is split into fragments using restriction enzymes . (encyclopedia.com)
This obstacle has been overcome by the field of recombinant DNA technology, which enables the preparation of more managable (i.e., smaller) DNA fragments. (addgene.org)
These tools are essential to the field of recombinant DNA, in which many identical DNA fragments can be generated. (addgene.org)
Ligase is chiefly used to fall in two DNA fragments and the procedure called as ligation. (free-essays.us)
Single bands of homology to the probe were also found in EcoRI fragments of chromosomal DNA obtained from 33 encapsulated, nontype b H. influenzae. (jci.org)

DNA barcoding technology offers the best technique for this to date, involving specific amplification of a short, standardized DNA fragment with universal primers across multiple samples [ 19 , 20 ]. (hindawi.com)
A 4.4-kb EcoRI fragment, common to both DNA clones, was used to characterize clinical isolates representing all six encapsulated serotypes as well as several capsule-deficient H. influenzae by Southern hybridization analysis. (jci.org)
The probe hybridized to an identical sized (4.4 kb) fragment of EcoRI-digested chromosomal DNA from eight independently isolated type b strains. (jci.org)

gene cloning ( DNA cloning ) The production of exact copies ( clones ) of a particular gene or DNA sequence using genetic engineering techniques. (encyclopedia.com)
Molecular Cloning is the process of producing recombinant DNA and transforming into host organisms to replicate and make more copies. (promega.com)

However, one of the factors that make it difficult and raise the price of production in every system consists in the purification process of the recombinant antigen. (scielo.br)

Guidelines for the use and safety of genetic engineering techniques or recombinant DNA technology. (who.int)

This procedure of section is called a DNA palindrome which explains the both strands have same nucleotide sequence but antiparallel placement. (free-essays.us)

Using a well-characterized rat model of H. influenzae systemic infection, we showed that type b transformants elicited by the cloned DNA were pathogenic, causing bacteremia and meningitis, whereas the untransformed capsule-deficient H. influenzae organisms were not. (jci.org)

Once you have designed the cloning scheme, gathering all of the required reagents to get you from construct to expression and analysis is not trivial. (promega.com)
Molecular cloning of DNA was used to investigate the expression of type b capsule and its association with H. influenzae virulence. (jci.org)

The use of cloning is interrelated with Recombinant DNA in classical biology, as the term "clone" refers to a cell or organism derived from a parental organism, [1] with modern biology referring to the term as a collection of cells derived from the same cell that remain identical. (wikidoc.org)

The complementary DNA is prepared from messenger RNA of retroviruses utilizing particular enzyme contrary RNA polymerase. (free-essays.us)
The identity of the clone and the accuracy of the sequence was verified by direct aa sequencing of the tryptic peptides derived from the purified rat liver enzyme. (lu.se)

J) describe unique elements of the molecular structure of a chromosome such as short tandem repeats (STR), transposons, and methylation and acetylation of DNA. (tx.us)
The deletion and duplication result in the recombinant 8 chromosome. (medlineplus.gov)
The signs and symptoms of recombinant 8 syndrome are related to the loss and addition of genetic material on these regions of chromosome 8. (medlineplus.gov)
which means one copy of the recombinant chromosome 8 in each cell is sufficient to cause the disorder. (medlineplus.gov)
Most people with recombinant 8 syndrome have one parent with a change in chromosome 8 called an inversion . (medlineplus.gov)
the resulting piece of DNA is reversed and reinserted into the chromosome. (medlineplus.gov)
People with the chromosome 8 inversion are at of risk having a child with recombinant 8 syndrome. (medlineplus.gov)
Oren MS, Camacho JE, Xie H, Lowe J, Cushing T, Clericuzio C, Maxwell JR. Postnatal diagnosis of de novo complex der(8) in a boy with prenatal diagnosis of recombinant chromosome 8 syndrome. (medlineplus.gov)

Should have unique restriction sites as polylinkers or multiple cloning site (MCS). (biologyexams4u.com)
Recombinant DNA technology was made possible by the discovery of restriction endonucleases by Werner Arber , Daniel Nathans , and Hamilton Smith , for which they received the 1978 Nobel Prize in Medicine. (wikidoc.org)

EC 2.1.1.6) was determined from rat cDNA and genomic libraries were screened with DNA probes and specific antiserum. (lu.se)

A virulent H. influenzae type b strain was used to construct a lambda library of chromosomal DNA in Charon 4. (jci.org)

Scientists have harnessed these tiny structures to carry foreign DNA into host cells, making them a crucial tool in biotech research. (biotecnika.org)
Interdisciplinary and integrated-systems research has taken on increased importance in science in the past decade as scientists tackle complex and socially important multidisciplinary problems such as disease spread and resistance, cloning technologies, and climate change. (carleton.edu)

The recombinant DNA techniques were foremost demonstrated in 1972, the parent field of molecular biological science was about entirely an academic field of research. (free-essays.us)

Cloning of chromosomal DNA from Haemophilus influenzae. (jci.org)

Anatomy8

Organisms16

Diseases2

Chemicals and Drugs32

Analytical, Diagnostic and Therapeutic Techniques and Equipment23

Phenomena and Processes46

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Humanities1

Information Science5

Health Care3

Proteins11

Vectors21

Plasmids8

Plasmid17

Genes4

Replication6

Antibody4

Genomics1

Antigens1

Molecule3

Molecules3

Phage1

Deoxyribonucleic3

Sequences4

Viral2

19872

Technology and genetic2

Genetics2

Coli2

Genome3

Genomes1

Vector3

Insulin2

Biotechnology5

Fragments5

Fragment3

Copies2

Antigen1

Genetic Engineering1

Nucleotide1

Pathogenic1

Expression2

Biology1

Enzyme2

Chromosome8

Restriction2

CDNA1

Construct1

Scientists2

Techniques1

Chromosomal1