• Chinese Hamster Ovary (CHO) cells are the leading mammalian cell platform for manufacturing therapeutic proteins due to their ability for producing human-like products. (manchester.ac.uk)
  • As a result, reducing waste product production through down-regulating GDPH, an enzyme directly related with glycerol production, as well as overexpressing TCA enzymes such as isocitrate dehydrogenase and aconitase, could potentially increase carbon flux, reduce waste products production and potentially increase productivity of recombinant proteins. (manchester.ac.uk)
  • Chinese hamster ovary and murine myeloma NS0 cells are currently favored host cell types for the production of therapeutic recombinant proteins. (kent.ac.uk)
  • By manipulation of intracellular nucleotide-sugar content, we examined the feasibility of implementing metabolic control strategies aimed at reducing the occurrence of murine-specific glycan motifs on NS0-derived recombinant proteins, such as Galalpha1,3Galbeta1,4GlcNAc. (kent.ac.uk)
  • Chinese hamster ovary (CHO) cells are one of the most useful host cell lines for the production of biopharmaceutical proteins. (elsevierpure.com)
  • Our catalog recombinant proteins are mainly related to cancer research and neurosciences. (eurogentec.com)
  • Few balancing acts are as delicate as the optimization of a cell expression system, especially if the expression system is to yield recombinant proteins destined for therapeutic use. (genengnews.com)
  • Further, there were no significant changes in metabolites or increases in biomass such as cell debris and host proteins. (genengnews.com)
  • CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. (rndsystems.com)
  • COSMOS medium is dedicated to recombinant protein expression for human like glycosylation, for example Erythropoietin, antibodies, Fc-fusion proteins. (thomassci.com)
  • Transient gene expression in mammalian cells provides reasonable amounts of recombinant proteins and antibodies which is sufficient for most applications. (invivo.de)
  • In this case, production from stable cell lines is highly advantageous since it allows easy generation of larger quantities of recombinant proteins and antibodies in measures of up to several grams per batch. (invivo.de)
  • All these combined with a highly optimized process for the selection of best-producing cell pools and screening for high-producing monoclonals, lead to cost-optimized production of target proteins in large quantities. (invivo.de)
  • This approach is beneficial especially for time-sensitive projects, as recombinant antibodies and proteins become available within just a few weeks' time. (invivo.de)
  • Animal cell technology is an area of rapid expansion and one that produces a wide range of high-value products, including vaccines, recombinant proteins, drugs for cardiovascular, respiratory and immune diseases, and monoclonal antibodies. (imperial.ac.uk)
  • Chimeric antigen receptors (CAR) recognize specific proteins on the surface of tumor cells. (msdmanuals.com)
  • In contrast to TCR T cells, CAR T cells recognize only relatively large proteins on the surface of tumor cells. (msdmanuals.com)
  • Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)
  • Purification of recombinant human growth hormone (rhGH) from Chinese hamster ovary (CHO) cell culture supernatant by Gradiflow large-scale electrophoresis is described. (edu.au)
  • To this end, ASIC1a current amplitudes and charge transport in transfected Chinese hamster ovary cells, and ASIC-mediated action potential signaling in cultured cortical neurons were measured in response to defined pH ramps of 1-40 s duration from pH 7.4 to pH 6.6 or 6.0. (frontiersin.org)
  • After years of optimization work to counteract these bottlenecks, InVivo successfully established a proprietary platform for the development of stable cell lines using CHO (Chinese hamster ovary) cells as expression system. (invivo.de)
  • This application note describes the cultivation of Chinese hamster ovary (CHO) suspension cells in the Finesse SmartGlass vessel bioreactor with a maximum working volume of 2.0 L. Using chemically defined minimal media, cell densities of up to 7.44 106 cells/mL were achieved. (bioprocessintl.com)
  • Enzyme replacement therapy (ERT) with glucocerebrosidase purified from human placenta was FDA approved in 1991, followed by approval in 1994 of a recombinant form of the enzyme produced in cultured Chinese hamster ovary (CHO) cells, marketed as imiglucerase (Cerezyme). (medscape.com)
  • In the commercial setting, hiPSCs start as single cells and are expanded into a Master Cell Bank (MCB) to create a therapeutic cell population. (aicompanies.com)
  • When the therapeutic you're developing is cell-based, constant monitoring to optimize growth and ensure consistent culture conditions throughout multiple stages is critical for success. (aicompanies.com)
  • Its recombinant form, produced through advanced biotechnological methods, offers several advantages for therapeutic and research purposes. (prospecbio.com)
  • These investigations may provide insights into the use of recombinant transferrin as a therapeutic agent in various clinical settings. (prospecbio.com)
  • Recombinant therapeutic protein expression using mammalian platforms can be achieved using a range of workflows optimized for different objectives. (lonza.com)
  • Identifying these changes can help us understand how diseases such as epilepsy affect these cells and may represent viable therapeutic targets. (dcu.ie)
  • There is growing appreciation that T-cells exhibit polyfunctionality by producing multiple cytokines simultaneously. (dcu.ie)
  • This thesis focused on characterizing the central carbon metabolism of different recombinant CHO cells to identify novel molecular mechanisms by increasing the metabolic load overexpressing human erythropoietin (EPO). (manchester.ac.uk)
  • The ED(50) was determined by the dose-dependentproliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of.0-10.0 ng/ml. (reprokine.com)
  • Recombinant full length protein within Human PD-L1. (abcam.com)
  • PeproTech's CHO cell-derived Recombinant Human sCD8α is a monomeric glycoprotein of 161 amino acid residues, which corresponds to the extracellular domain of CD8α. (peprotech.com)
  • hiPSC (human induced pluripotent stem cell) are a source of potential cellular immunotherapies. (aicompanies.com)
  • When Recombinant Human CD200 Fc Chimera (Catalog # 2724-CD ) is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human CD200R1 Fc Chimera Avi-tag (Catalog # AVI10678) that produces 50% of the optimal binding response is appoximately 2.5-15 ng/mL. (rndsystems.com)
  • In direct ELISAs, no cross-reactivity with recombinant mouse CD39, recombinant human CD39L2, 3, or 4 is observed. (rndsystems.com)
  • Recombinant Human Transferrin produced in CHO cells is a glycosylated, polypeptide chain containing having a molecular mass of 76 kDa. (prospecbio.com)
  • Human recombinant transferrin, a glycoprotein responsible for iron transport in the body, has gained increasing attention in the fields of biomedicine and health sciences. (prospecbio.com)
  • This study aims to provide a comprehensive exploration of human recombinant transferrin, shedding light on its various functions and potential applications in health and biomedicine. (prospecbio.com)
  • The second objective is to assess the clinical relevance of human recombinant transferrin in medical interventions. (prospecbio.com)
  • The third objective is to explore the broader implications of human recombinant transferrin in biomedicine and research. (prospecbio.com)
  • By delving into the diverse functions of human recombinant transferrin, this research aims to expand our understanding of its physiological roles and clinical applications. (prospecbio.com)
  • Recombinant SEAP (secreted embryonic alkaline phosphatase) protein is a truncated form of human placental alkaline phosphatase that comprises 520 amino acids. (invivogen.com)
  • 10 μg lyophilized recombinant human secreted embryonic alkaline phosphatase (SEAP). (invivogen.com)
  • Specifically, the company's technologies will be applied to human (HEK-293) and mammalian (CHO) cell lines. (ddw-online.com)
  • Antagonist activity at human recombinant adrenergic Alpha-2C receptor expressed in CHO cells assessed as inhibition of NE-induced calcium mobilizatio. (bindingdb.org)
  • Antagonist activity at human recombinant adrenergic alpha2B receptor expressed in CHO cells assessed as inhibition of NE-induced calcium mobilization. (bindingdb.org)
  • This form of recombinant enzyme is produced in cultured human cells and is marketed as velaglucerase alfa (VPRIV). (medscape.com)
  • These results raise concerns of whether subclinically infected adult swine may be reservoirs of new human NoVs or if porcine/human GII recombinants could emerge. (cdc.gov)
  • Effect of recombinant human bone morphogenetic protein-4 with carriers in rat calvarial defects. (bvsalud.org)
  • 10. Marden LJ, Hollinger JO, Chaudhari A, Turek T, Schaub RG, Ron E. Recombinant human bone morphogenetic protein-2 is superior to demineralized bone matrix in repairing craniotomy defects in rats. (bvsalud.org)
  • CAR T cell immunotherapy for human cancer. (msdmanuals.com)
  • In this study, we compared N-glycan processing in GS-NS0 and GS-CHO cells producing the same model recombinant glycoprotein, tissue inhibitor of metalloproteinases 1. (kent.ac.uk)
  • Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. (peprotech.com)
  • Catalyzes hydrolytic cleavage of glucocerebroside (a glycoprotein) to glucose and ceramide within the lysosomes of phagocytic cells in the reticuloendothelial system. (medscape.com)
  • Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
  • Microfluidic cell sorting with the WOLF, along with single cell deposition using the N1 single cell dispenser, eliminates the challenges that pluripotent stem cells face by sorting at gentle pressures without decompression shock and shear stress, and allow for efficient single cell deposition for high monoclonal outgrowth. (mscience.com.au)
  • When you use monoclonal antibodies from hybridoma cell lines that do not promise the expected yields, then this does not have to be the end of your project. (invivo.de)
  • The CHO cell line secretes recombinant factor IX into a defined cell culture medium that does not contain hormones, and the recombinant factor IX is purified by a chromatography purification process that does not require a monoclonal antibody step. (globalrph.com)
  • Mammalian cells are currently the preferred expression system for high-value bio-pharmaceutical products, in particular, monoclonal antibodies. (imperial.ac.uk)
  • Cell culture is widely used for stable expression of monoclonal antibodies with posttranslational modifications. (bioprocessintl.com)
  • In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. (rndsystems.com)
  • The 4x and 10x objectives can obtain images of cells grown on standard tissue culture well plates. (sheffield.ac.uk)
  • Research will investigate its potential roles in areas beyond iron transport, such as drug delivery, tissue engineering, and cell culture. (prospecbio.com)
  • It has become clear, however, that the susceptibility of cells to infection with TSE agents cannot be reliably predicted either from their tissue of origin or level of expression of PrP C ( 11 , 12 ). (cdc.gov)
  • Could you elaborate on the methods used to study IL17C's contribution to tissue-specific immune cell recruitment? (creativebiomart.net)
  • I have recently shown that synovial tissue poly-T-cells can predict RA-patient stratification, correlate with disease progression in RA and PsA and importantly, are present at the joints of at risk-individuals prior to development of clinical-inflammation. (dcu.ie)
  • tPA produced by such means are referred to as recombinant tissue plasminogen activator ( rtPA ). (wikidoc.org)
  • Recombinant beta-glucocerebrosidase (imiglucerase [Cerezyme]) has replaced the original tissue-derived product, alglucerase (Ceredase). (medscape.com)
  • The large proteoglycan molecules made up of protein cores, and GAG branches are secreted by cells and constitute a significant fraction of the extracellular matrix of connective tissue. (medscape.com)
  • The enzyme deficiencies lead to the accumulation of mucopolysaccharides in the lysosomes of the cells in the connective tissue and to an increase in their excretion in the urine. (medscape.com)
  • However, the progenitor cells consist of T cells isolated from resected tumor tissue. (msdmanuals.com)
  • Current understanding of mammalian cell cultures lies in the association of their metabolic behaviour to their antibody production output. (imperial.ac.uk)
  • The main objective of the project is to study the effects of hyperosmotic pressure on the antibody production process in the GS-NS0 cell line, with particular attention to the antibody production mechanism. (imperial.ac.uk)
  • These data suggest that manipulation of nucleotide-sugar metabolism can promote changes in N-glycan processing that are either conserved between NS0 and CHO cells or specific to either NS0 cells or CHO cells. (kent.ac.uk)
  • The company is currently evaluating the serum-free supplement on other aspects of bioprocessing, including single-use bioreactors, protein quality, and cell metabolism. (genengnews.com)
  • New avian suspension cell lines provide production of influenza virus and MVA in serum-free media: studies on growth, metabolism and virus propagation. (probiogen.de)
  • Two fundamentals aspects of cell behaviour any model must include are metabolism and the cell cycle. (imperial.ac.uk)
  • The population balance framework is used to capture cell cycle effects while a single-cell model takes account of some features of intracellular metabolism. (imperial.ac.uk)
  • This study demonstrates the ability of Gradiflow preparative electrophoresis technology to purify rhGH from mammalian cell culture supernatant in a one-step process with high purity and yield. (edu.au)
  • Our research programme sets out to integrate modelling, experiment design and validation, and control and optimisation into a single framework that would lead to increased productivity, regulated product quality, and reduced costs for mammalian cell culture systems. (imperial.ac.uk)
  • The integration of these three research tools represents a unique, novel, and interdisciplinary approach to addressing the complicated research and industrial problem of model-based control and optimisation of mammalian cell culture processes. (imperial.ac.uk)
  • A recombinant-derived analog of beta-glucocerebrosidase produced in mammalian cell culture and chemically modified by mannose termination of glycosylated amino acids. (medscape.com)
  • Production of rhGH in CHO cells is an alternative to production in Escherichia coli, with the advantage that rhGH is secreted into protein-free production media, facilitating a more simple purification and avoiding resolubilization of inclusion bodies and protein refolding. (edu.au)
  • Purification of modified vaccinia virus Ankara from suspension cell culture. (probiogen.de)
  • Scientists at the University of Sheffield looked to overcome this hurdle by developing an automated small-scale analytical platform workflow, combining purification and aggregation analysis of protein biopharmaceuticals expressed in 96 deep well plate cell cultures.4 Their workflow utilized a liquid handler equipped with a protein-A PhyTip column for purification and a size-exclusion chromatography (SEC) column for aggregate monitoring. (technologynetworks.com)
  • RECOTHROM thrombin is available as 5000-unit and 20,000-unit vials of sterile recombinant topical thrombin lyophilized powder for solution. (drugs.com)
  • Better than a fluidic cart, sterile microfluidic cartridges ensure low-pressure cell sorting applications for sensitive cells. (mscience.com.au)
  • Recombinase-mediated site-specific gene integration into a predetermined chromosomal locus may enable predictable protein expression, reducing the laborious process of cell screening. (elsevierpure.com)
  • Ensure stem cells are at the highest viability and product quality levels throughout the entire cell therapy workflow from culture and processing all the way to fill and finish. (aicompanies.com)
  • Although a series of production processes have been refined to improve protein productivity and cost performance, establishing producer cells is still time-consuming and labor-intensive. (elsevierpure.com)
  • When developing a stable cell line, the resulting cell pool then serves as starting point for single cell cloning and various screening processes, in which the clone with the highest productivity is identified and selected as basis for the stable cell line. (invivo.de)
  • Sigma-Aldrich Biotechnology used mixture-DOE to increase cell growth and productivity for a particular recombinant CHO clone. (statease.com)
  • A successful cell line has high productivity. (technologynetworks.com)
  • Vaccine adjuvant formulations using aluminum salts or "alum" may be particularly effective for technologies using inactivated COVID-19 virus and for recombinant protein-based or vector-based vaccines. (wikipedia.org)
  • Continuous cell lines from the Muscovy duck as potential replacement for primary cells in the production of avian vaccines. (probiogen.de)
  • Through mRNA administration, vaccines prompt your cells to produce a harmless version of the spike protein, thereby stimulating your immune system to produce antibodies to fight off the potential threat. (moleculardevices.com)
  • 2 μg/lane of Biotinylated Recombinant CD200R1 Fc Chimera Avi-tag Protein (Catalog # AVI10678) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 80-100 kDa and 160-200 kDa, respectively. (rndsystems.com)
  • Metabolic profiling of recombinant CHO cells to identify potential targets for cell line engineering. (manchester.ac.uk)
  • Metabolomics data obtained through gas chromatography mass spectrometry (GS-MS) of a tetracycline (tet) inducible CHO T-RExTM cell line capable of expressing 0.09 pg/ul/cell EPO, revealed that 0.1% v/v tet addition does not affect consumption of carbon sources such as glucose, and pyruvate, but concentration of waste products, such as glycerol, threitol, alanine and phenyllactic acid increases towards the end of each batch culture. (manchester.ac.uk)
  • L2987 cell line. (abcam.com)
  • For negative control sample , cell line COLO205 is recommended. (abcam.com)
  • Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. (sigmaaldrich.com)
  • The N1 single-cell dispenser is ideal for a multitude of applications including single cell cloning and works seamlessly with the microfluidic-based WOLF cell sorter for easy single cell isolation, single cell analysis, and cell line development. (mscience.com.au)
  • However, developing a stable cell line can be a time and resource intensive process with several bottlenecks that need to be overcome in order to ensure a good yield output. (invivo.de)
  • We chose a well-established, cGMP-banked CHO cell line and combined it with our optimized cell culture processes using a high-performance, chemically-defined, animal component-free medium. (invivo.de)
  • After detailed rounds of discussion with our scientific experts, we help you decide if a stable cell line needs to be developed or if we can produce the required protein yields from a stable cell pool. (invivo.de)
  • In many cases, sufficient material can be obtained through production from stable CHO cell pools instead of going all the way to produce a stable cell line. (invivo.de)
  • To circumvent this problem and speed up research and development, a large part of the pre-clinical studies can be carried out using InVivo's CHO cell pool concept based on a well-established cGMP-banked cell line, which can later be transferred into GMP facilities. (invivo.de)
  • RIXUBIS is produced by a genetically engineered CHO cell line. (globalrph.com)
  • No PrP TSE or infectivity has been detected in any exposed cell line under study so far. (cdc.gov)
  • The avian cell line AGE1.CR.pIX characterized by metabolic flux analysis. (probiogen.de)
  • NewBiologix uses next-generation sequencing (NGS)-based genome characterisation to develop a proprietary bioinformatics platform designed to improve cell line capabilities for the expression and production of ATMPs. (ddw-online.com)
  • 11. Flamigni F., Campana G., Carboni L., Guarnieri C., Spampinato S., Zinc is required for the expression of ornithine decarboxylase in a difluoromethylornithine-resistant cell line. (unibo.it)
  • 13. Flamigni F., Campana G., Carboni L., Rossoni C., Spampinato S., Post-trascriptional inhibition of ornithine decarboxylase induction by zinc in a difluoromethylornithine resistant cell line. (unibo.it)
  • It is a serine protease ( EC 3.4.21.68 ) found on endothelial cells , the cells that line the blood vessels . (wikidoc.org)
  • While new technologies enable the engineering of high titer cell clones, there is still a need to screen these clones quickly.1 The current paradigm for titer analysis depends on chromatographic approaches which, even with the newest ultra-high-performance liquid chromatography (UHPLC) models, will take minutes to analyze one cell line. (technologynetworks.com)
  • Despite having a stable cell line, a protein product is still in danger of improper modifications from chemical interactions with media. (bioprocessintl.com)
  • This process theoretically provides a line of T cells with greater tumor specificity than those obtained from the blood. (msdmanuals.com)
  • RIXUBIS [Coagulation Factor IX (Recombinant)] is a purified protein produced by recombinant DNA technology. (globalrph.com)
  • Each vial contains nominally 250, 500, 1000, 2000 or 3000 international units of recombinant coagulation factor IX. (globalrph.com)
  • Take a sample of the cell suspension, e.g. 100μl, to count cells. (sigmaaldrich.com)
  • Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. (sigmaaldrich.com)
  • The medium is developed for recombinant protein production in suspension cells. (thomassci.com)
  • High cell density cultivations by alternating tangential flow (ATF) perfusion for influenza A virus production using suspension cells. (probiogen.de)
  • Live attenuated influenza viruses produced in a suspension process with avian AGE1.CR.pIX cells. (probiogen.de)
  • Recombinant SEAP protein is purified by affinity chromatography. (invivogen.com)
  • Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps. (origene.com)
  • Six samples were positive for NoV. Based on sequence analysis of 3 kb on the 3´ end of 5 porcine NoVs, 3 genotypes in GII and a potential recombinant were identified. (cdc.gov)
  • NTPDase-1 was originally described as CD39, a B lymphocyte cell surface marker (2), but it is also present on the surface of natural killer cells, T cells, and some endothelial cells (3). (rndsystems.com)
  • The objective of this study was to determine the potential susceptibility to infection of 5 cell lines used or proposed for manufacture of biological products, as well as other lines. (cdc.gov)
  • The company's DNA-based platform is being developed to address manufacturing challenges associated with the production of recombinant adeno-associated virus (rAAV) vectors used to produce advanced therapy medicinal products (ATMPs). (ddw-online.com)
  • Successful cell culture on a large scale requires close control of the culture environment in terms of temperature, pH, removal of waste products, and addition of fresh nutrients. (bioprocessintl.com)
  • Here, we attempted to enhance transgene expression in recombinant CHO cells generated by AGIS using a production enhancer DNA element (PE) derived from the CHO genome. (elsevierpure.com)
  • These results indicate that AGIS using the PE-flanked expression unit is a promising approach for establishing producer cell lines for biopharmaceutical protein production. (elsevierpure.com)
  • THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO 2 production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO. (sigmaaldrich.com)
  • Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. (peprotech.com)
  • The production of MCBs must adhere to cGMP (Clinical Good Manufacturing Process) conditions, aiming to optimize cell growth while ensuring their stability and functionality for safe and effective transplantation into patients. (aicompanies.com)
  • Developed to maximize CHO-S cells recombinant protein production. (thomassci.com)
  • During stable transfection, the DNA of interest is integrated into the genome of the host cell to allow steady production of the target protein. (invivo.de)
  • By skipping the lengthy screening procedures and continuing with production directly from the cell pool, it is possible to reduce the costs and timeline of your project without compromising on the quality of the product. (invivo.de)
  • To obtain these custom modified antibodies in large quantities and quick turnaround times, production in stable CHO cells is ideal and highly recommended. (invivo.de)
  • Transient and stable pool processes are generally used for discovery through early-stage development to enable rapid production and screening of large numbers of protein candidates, while stable clonal cell lines are used for GMP clinical and commercial manufacturing. (lonza.com)
  • Studies showing that murine fibroblast cell lines are susceptible to infection with mouse-adapted scrapie agent ( 11 , 12 ) increased concern that nonneuronal cell substrates used to propagate viruses for vaccine production might become infected with a TSE agent contaminating some component of culture medium, especially bovine serum ( 13 ). (cdc.gov)
  • Continuous influenza virus production in cell culture shows a periodic accumulation of defective interfering particles. (probiogen.de)
  • Cell clones that produce high titers allow for the production of drugs in a higher quantity but in less time. (technologynetworks.com)
  • Recombinant Vascular endothelial growth factor is a homodimer protein consisting ofx165 amino acid residue subunits, and due to glycosylation migrates as an approximately kDa protein under non-reducing and askDa under reducing conditions in SDS-PAGE. (reprokine.com)
  • 5. Spampinato S., Bachetti T., Carboni L., Ratti E., Van Amsterdam F.T.M., Ferri S., Ca2+ Channel blocking activity of lacidipine and amlodipine in A7r5 vascular smooth muscle cells. (unibo.it)
  • Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. (peprotech.com)
  • A number of immunologic interventions, both passive and active, can be directed against tumor cells. (msdmanuals.com)
  • Passive cellular immunotherapy references A number of immunologic interventions, both passive and active, can be directed against tumor cells. (msdmanuals.com)
  • Tumor Antigens Many tumor cells produce antigens, which may be released in the bloodstream or remain on the cell surface. (msdmanuals.com)
  • TAAs) with high specificity to tumor cells. (msdmanuals.com)
  • Concomitant use of interferon enhances the expression of major histocompatibility complex (MHC) antigens and TAAs on tumor cells, thereby augmenting the killing of tumor cells by the infused effector cells. (msdmanuals.com)
  • T-cell engagers are bispecific antibodies that recruit cytotoxic T cells to kill tumor cells. (msdmanuals.com)
  • Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. (rndsystems.com)
  • Using these customized vectors, high protein yields and a substantially reduced timeline from transfection to stable CHO cell pools could be achieved. (invivo.de)
  • These processes include transient transfection, and the creation of stable pools and clonal cell lines. (lonza.com)
  • Recombinant IL-6 is a glycosylated protein consisting of 184 amino acid residue subunits, due to glycosylation migrates as an approximately 30kDa protein under reducing conditions in SDS-PAGE. (reprokine.com)
  • About NanoCellect's microfluidic flow cytometry platforms enable biomedical scientists to analyse and sort cells required for drug discovery, diagnostics, and basic research. (mscience.com.au)
  • How are the effects of IL17C modulation on specific immune cell populations characterized using flow cytometry and cell sorting? (creativebiomart.net)
  • Flow cytometry analysis with cell-specific markers characterizes IL17C's impact on immune cell subsets. (creativebiomart.net)
  • THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. (sigmaaldrich.com)
  • In vitro and in vivo experiments will be conducted to investigate how recombinant transferrin interacts with cellular receptors, regulates iron uptake, and influences cellular proliferation. (prospecbio.com)
  • In SARS-CoV-2, the aim is to disrupt the binding of the spike protein with ACE2 receptors, prohibiting the entry of the virus into the host cells. (moleculardevices.com)
  • To further increase product yield, high cell-density fermentation processes were established. (invivo.de)
  • These EVs can mediate signalling to cells throughout the body, affecting major cell processes. (dcu.ie)
  • Models of animal cell culture systems have a wide range of potential applications, such as analysis and prediction of experimental results, optimisation of culture conditions for prolonged viability, and perhaps most importantly, the investigation of fundamental metabolic processes and their subsequent elucidation. (imperial.ac.uk)
  • To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. (sigmaaldrich.com)
  • For example, with animal cell cultures, the trend is away from serum and toward defined media, that is, media uncomplicated by the presence of animal-origin components. (genengnews.com)
  • Cluster of Differentiation-200 receptor 1 (CD200R1), also known as OX-2 receptor, is a transmembrane protein in the immunoglobulin superfamily expressed on the surface of myeloid cells and important in the regulation of myeloid cell activity (1-3). (rndsystems.com)
  • Reduced stress via gentle microfluidic cell sorting (low throughput) process also avoids potential adverse gene expression or karyotype changes experienced with a traditional cell sorter. (mscience.com.au)
  • Propagation of viruses infecting waterfowl on continuous cell lines of Muscovy duck (Cairina moschata) origin. (probiogen.de)
  • We previously developed an accumulative site-specific gene integration system (AGIS) using Cre recombinase and mutated loxP sites for transgene integration and amplification in the CHO cell genome. (elsevierpure.com)
  • If combined with COSMOS Feeds medium supports a viable cell concentration up to 3x107/mL. (thomassci.com)
  • In order to trigger gene amplification of both genes, DG44-I and DG44-C cells were incubated and sub cultured in the presence of 250 nM MTX for 35 days, leading to EPO titers of 0.123 and 0.15 pg/ul/cell respectively without affecting maximum viable cell density (VCD). (manchester.ac.uk)
  • It is clear that the biological behaviour of cells is of such complexity that any model, to be robustly predictive, must be sufficiently structured and detailed in terms of the captured biology. (imperial.ac.uk)
  • Taliglucerase is a plant-based recombinant enzyme. (medscape.com)
  • Additionally, two DG44 cell lines containing inducible (pcDNA4/TO) or constitutive (pCDNA3.1) vectors (DG44-I and DG44-C cells respectively) with EPO and DHFR genes were generated. (manchester.ac.uk)
  • Glycerol, citrate and isotritate significantly increased in all the cell lines assessed upon EPO expression. (manchester.ac.uk)
  • In addition, elevated UDP-N-acetylhexosamine content was associated with a slight decrease in sialylation in both cell lines. (kent.ac.uk)
  • Cell lines were exposed to the infectious agents of sporadic and variant Creutzfeldt-Jakob disease and bovine spongiform encephalopathy (BSE). (cdc.gov)
  • All cell lines we studied resisted infection with 3 TSE agents, including the BSE agent. (cdc.gov)
  • Impaired antiviral response of adenovirus-transformed cell lines supports virus replication. (probiogen.de)
  • New South African company NewBiologix has launched with the aim of developing a proprietary and breakthrough platform for the advanced engineering of cell lines used to manufacture gene and cell therapies. (ddw-online.com)
  • A critical need is to speed up analytical times while scaling down cell culture systems, to help better assess cell lines for key attributes while decreasing the costs of goods manufactured. (technologynetworks.com)
  • A dilution series of the recombinant Lucia luciferase protein can also be used to determine the linear range of the assay. (invivogen.com)
  • When combined with the N1 Single-Cell Dispenser accessory, it allows higher rates in single cell detection in wells compared to cell printers or limited dilution methods. (mscience.com.au)
  • As the number of gene and cell therapies in the clinic and market continues to grow, improved manufacturing solutions for viral vectors are needed," said co-founder and CEO, Igor Fisch. (ddw-online.com)
  • NewBiologix is currently focused on recombinant adeno-associated virus (rAAV) vectors, the preferred delivery vehicle for gene therapies. (ddw-online.com)
  • as a result, the current industrial control of hybridoma cell culture is still fundamentally manual. (imperial.ac.uk)
  • Each vial of RIXUBIS has the recombinant Factor IX (rFIX) potency in international units stated on the vial. (globalrph.com)
  • Inclusion of the nucleotide-sugar precursors, glucosamine (10 mM, plus 2 mM uridine) and N-acetylmannosamine (20 mM), in culture media were shown to significantly increase the intracellular pools of UDP-N-acetylhexosamine and CMP-sialic acid, respectively, in both NS0 and CHO cells. (kent.ac.uk)
  • Add thawed cells to a conical based centrifuge tube, e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. (sigmaaldrich.com)
  • Every expression system-every scheme for optimizing nutrients and cell culture conditions-presents advantages, disadvantages, and tradeoffs with respect to costs, yields, quality metrics, contaminants, availability of system components, time constraints, and so on. (genengnews.com)
  • Use of serum to culture cells dates back nearly 70 years and continues to this day, albeit with less devotion. (genengnews.com)
  • Essential Pharma focused on filling in the hole in this cell culture void. (genengnews.com)
  • To facilitate cell culture optimization, Essential Pharma has developed Cell-Ess, a serum replacement that is free of animal-origin components. (genengnews.com)
  • Immunoassay, cell culture. (prospecbio.com)
  • During early-stage development, identifying aggregation in scale-down cell culture systems can be tedious, as the small sample volumes make aggregate detection challenging. (technologynetworks.com)
  • For testing in cell culture applications, please filter before use. (origene.com)
  • Lymphokine-activated killer (LAK) cells are produced from the patient's T cells which are extracted from the tumor and grown in a cell culture system with the lymphokine interleukin-2 (IL-2). (msdmanuals.com)
  • These cells are grown in culture in a manner similar to LAK cells. (msdmanuals.com)
  • First, we in order to generate the relevant constructs for stable expression in CHO cells. (invivo.de)