Recombinant cas9 protein. Medical search. Frequent questions

Guide-it recombinant Cas9 is a recombinant wild-type Streptococcus pyogenes Cas9 nuclease modified with a C-terminal nuclear localization signal (NLS) for use in CRISPR/Cas9-mediated gene-editing experiments. (takarabio.com)
Polyclonal antibody raised in rabbit against the Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein. (diagenode.com)
Monoclonal antibody raised in mouse against the N-terminus of the Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein. (diagenode.com)
Alternative name: Csn1 Monoclonal antibody raised in mouse against the N-terminus of the Cas9 nuclease (CRISPR-associated protein 9) using a recom. (diagenode.com)
Polyclonal antibody raised in rabbit against the C-terminus of the S. aureus Cas9 nuclease (CRISPR-associated protein 9) using a recombinant protein. (diagenode.com)
Order your Recombinant CRISPR Cas9 Protein and AsCas12a (CRISPR-associated nuclease Acidaminococcus sp. (lifetein.com)
The best known and most widely used Cas nuclease is Cas9. (idtdna.com)
This version of Cas9, known as Alt-R Cas9 Nuclease V3, is an excellent choice for most genome editing experiments. (idtdna.com)
If you need a Cas9 with reduced off-target activity that still has the potency (efficiency) of wild-type Cas9, we additionally offer Alt-R HiFi Cas9 Nuclease, which works well when delivered as an RNP , has very high efficiency, and causes almost no off-target effects . (idtdna.com)
Cas9 nuclease, single guide RNA, and donor DNA were transfected into mouse cells. (eco-vector.com)
Suitable for challenging experimental conditions (e.g., high nuclease environments or with Cas9 mRNA). (idtdna.com)
The new Alt-R HiFi Cas9 Nuclease has similar on-target potency to wild-type S.p. (idtdna.com)
HiFi Cas9 nuclease. (idtdna.com)

From monoclonal antibodies and recombinant proteins to the most advanced cell and gene therapies, our comprehensive set of products and services will accelerate your workflows, reduce risk, and get you to the finish line faster. (sigmaaldrich.com)
Explore our vast selection of primary, secondary, and recombinant monoclonal antibodies for ELISA, Western blot, and immunohistochemistry (IHC). (sigmaaldrich.com)
Monoclonal Antibodies to Coronavirus SARS-CoV-2 RBD Domain Description :2019 Coronavirus SARS-CoV-2 Spike S1 RBD Protein, human IgG1 Fc tag. (lifetein.com)
B lymphocytes - or B cells - are the components of the immune system responsible for making antibodies: soluble proteins that seek out and eliminate foreign entities in the body, like viruses. (garvan.org.au)
This work is led by Dr David Zahra in the Genomic Engineering Group and is extending the use of CRISPR/Cas9 for gene therapy and production of therapeutic monoclonal antibodies. (garvan.org.au)

Early attempts to apply CRISPR-Cas9 for genome editing in CD34 + HSPCs used plasmids for Cas9 and guide RNA (gRNA) expression, which resulted in low targeting efficiency and high toxicity. (stemcell.com)
You can easily produce a recombinant lentivirus particle in HEK293T or HEK293FT cell line in high titer using GeneMedi's lentivirus Vector System, including the lentivirus expression plasmids (overexpression, shRNA or Crispr-cas9-gRNA), the envelope protein VSV-G expressing plasmid pMD2G and the packaging plasmid pSPAX2. (genemedi.net)

In this study, mouse conditional knockout of VSMC GGTase-I (Pggt1b Δ / Δ mice) was generated using the CRISPR/Cas9 system. (hindawi.com)
Recombinant LDLR variants were constructed using CRISPR/Cas9 system and were used to transfect HepG2 cells. (bvsalud.org)
DNA cleavage by the CRISPR-Cas9 system. (idtdna.com)
The Alt-R™ CRISPR-Cas9 System includes all the reagents needed for successful genome editing in your research applications based on the natural S. pyogenes CRISPR-Cas9 system. (idtdna.com)

CRISPR-Cas9, an RNA-guided genome editing technology, is revolutionizing cell biology due to the ease and efficiency by which it enables genetic manipulation of mammalian cells. (stemcell.com)
This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
Here we carried out CRISPR-Cas9-mediated genome-wide screens and identified the members of the Wnt receptor frizzled family (FZDs) as TcdB receptors. (nih.gov)
In CRISPR genome editing, scientists have repurposed Cas9 RNP to cut targeted sites in any genome. (idtdna.com)
Alt-R Cas9 enzymes from IDT provide high-potency precise genome editing. (idtdna.com)
This endonuclease activity works the same way in different experimental organisms and cell lines in the lab, so Cas9 can be used for genome editing. (idtdna.com)
This is one frequent way in which scientists use Cas9 in genome editing. (idtdna.com)
IDT offers a couple major options for your Cas9 genome editing work. (idtdna.com)
As a result of the study, a strategy for CRISPR/Cas9-assisted homologous recombination in the genome of mouse embryonic stem cells has been developed to create a fully humanized Snca gene encoding α-synuclein, and the clone genome of mouse embryonic stem cells has been edited using a CRISPR technology. (eco-vector.com)
New genome editing approaches development using artificial nucleases (TALEN and CRISPR/Cas9). (viaf.org)
Selective gene dosage by CRISPR-Cas9 genome editing in hexaploid Camelina sativa. (viaf.org)
Cas9, but with significantly reduced off-target effects, allowing for precise genome editing, even under challenging conditions. (idtdna.com)
Genome replication and subgenomic RNA transcription after entry carry on with the participation of many nonstructural proteins such as Mpro (main protease or 3CLpro), PLpro (papain-like protease) and RdRp (RNA-dependent RNA polymerase). (medchemexpress.com)
Co-circulation of multiple subtypes of enterovirus A71 (EV- A71) genotype C, including novel recombinants characterised by use of whole genome sequencing (WGS), Denmark 2016. (cdc.gov)

Polyclonal antibody raised in rabbit against Lachnospiraceae bacterium (Lb) Cpf1 (CRISPR from Prevotella and Francisella 1) using a recombinant protein. (diagenode.com)
Polyclonal antibody raised in rabbit against CEBP Alpha (CCAAT/enhancer binding protein (C/EBP), alpha), using a KLH-conjugated synthetic peptide. (diagenode.com)
Polyclonal antibody raised in rabbit against human CHD1 (Chromodomain Helicase DNA Binding Protein 1), using a synthetic peptide containing a sequence from t. (diagenode.com)

Cas9 is guided to the target site in any DNA (viral DNA, mammalian genomic DNA, etc.) by short, complementary RNA sequences called CRISPR RNAs (crRNAs). (idtdna.com)
Herpesvirus of turkeys (HVT), used originally as a vaccine against Marek's disease (MD), has recently been shown to be a highly effective viral vector for generation of recombinant vaccines that deliver protective antigens of other avian pathogens. (mdpi.com)
In recent work, the lab demonstrated that the application of recombinant viral vectors to various models of storage disease reversed CNS deficits and improved life span. (chop.edu)

Geranylgeranyl transferase-I (GGTase-I) is one of the key enzymes mediating protein isoprenylation during the posttranslational modification, especially the geranylgeranylation of small GTPase, Rac1 [ 6 ]. (hindawi.com)

This self-amplifying mRNA encodes four nonstructural proteins (nsP1 to nsP4) derived from a positive-stranded alphavirus venezuelan equine encephalitis virus (VEEV), and NLS-eCas9-T2A-EGFP. (excellgen.com)
They can affect mRNA metabolism and protein synthesis by direct mRNA modifications or via tailing of regulatory noncoding RNAs (ncRNAs). (ceitec.eu)
The initial mRNA transcript, which is called a pre-mRNA molecule, includes both introns and exons, and the process of RNA splicing removes the introns to leave a mature mRNA transcript (composed only of exons) to be translated into protein. (scienceboard.net)
A single gene can give rise to multiple proteins though alternative splicing, where different introns are removed from the same pre-mRNA to create differently spliced mature mRNAs. (scienceboard.net)
The orally bioavailable, small-molecule drug modifies the splicing pattern of SMN2 mRNA to include exon 7, thereby increasing the expression of the full-length SMN protein. (scienceboard.net)

The single-guide RNA (sgRNA) approach uses a fused crRNA and tracrRNA, but otherwise the entire Cas9 system works like the two-part guide RNA system shown in panel A. (idtdna.com)
As mentioned above, the bacterial Cas9 system uses crRNA and tracrRNA together to form the guide RNA. (idtdna.com)

The researchers showed that both tissues had more chondroitin sulfate moieties of higher molecular weight than other tissues, concurrent with chondroitin polymerization proteins being upregulated in most cancer types. (asbmb.org)
In line with this, CHPF and CHPF2, which encode proteins required for chondroitin polymerization, are significantly upregulated in most cancer types. (lu.se)

NLS-eCas9 is a mutant of Streptococcus pyogenes SF370 Cas9 protein with nuclear localization signal (NLS) at N terminus. (excellgen.com)

TDP43 was first identified as a novel cellular protein that binds to HIV-1 virus TAR DNA sequence motifs and acts a transcriptional repressor to the HIV-1 LTR. (thermofisher.com)
Although the protein sequence showed high identity with plant and invertebrate MTs, it contained a unique 'linker ' region (14 amino acid residues) between the two putative metal-binding domains which contained no cysteine residues. (portlandpress.com)
If you want wild-type Cas9, we offer it as a recombinant protein fused with a proprietary nuclear localization sequence (NLS). (idtdna.com)
Guide RNAs (gRNAs) contain the target-specific sequence for guiding Cas9 protein to a genomic location. (idtdna.com)

Electroporation of mammalian cells with Cas9-sgRNA ribonucleoprotein complexes allows you to minimize off-target effects while facilitating a fast turnaround for highly efficient gene-editing experiments . (takarabio.com)
While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)
FZD1/2/7 triple-knockout cells are highly resistant to TcdB, and recombinant FZD2-CRD prevented TcdB binding to the colonic epithelium. (nih.gov)
Human PRB1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade. (antibodies-online.com)
This study explored the predictive impact of LDLR missense variants on protein structure and investigated their functional effects on LDLR expression in HepG2 cells transfected with CRISPR/Cas9 constructs. (bvsalud.org)
2019) Cartilage Oligomeric Matrix Protein initiates cancer stem cells through activation of Jagged1-Notch3 signaling. (lu.se)
An S protein mediates the CoV entry into host cells by attaching to a cellular receptor (ACE2 for SARS-CoV and SARS-CoV-2, DPP4 for MERS-CoV), followed by fusion between virus and host cell membranes. (medchemexpress.com)
SARS-CoV-2 S1 Protein (HEK293) is the recombinant S1 protein produced in HEK293 cells. (medchemexpress.com)
SARS-CoV-2 S Protein RBD (HEK293, His) produced in HEK293 cells is a recombinant SARS-CoV S protein receptor-binding domain (RBD) with His tag. (medchemexpress.com)
SARS-CoV-2 S1 Protein (HEK293, mFc) is the recombinant 2019-nCoV S1 protein with C-mFc tag produced in HEK293 cells. (medchemexpress.com)
Several receptors have been demonstrated for the interaction of spike (S) protein of SARS-CoV-2 with host cells, including angiotensin-converting enzyme (ACE2), ephrin ligands and Eph receptors, neuropilin 1 (NRP-1), P2X7, and CD147. (bvsalud.org)
Recombinant subfragments of VAR2CSA (rVAR2) have also been shown to bind specifically and with high affinity to cancer cells and tissues, suggesting the presence of a shared type of oncofetal chondroitin sulfate (ofCS) in the placenta and in tumors. (lu.se)
CRISPR/Cas9 targeting of CHPF and CHPF2 in tumor cells reduced the average molecular weight of cell-surface chondroitin sulfate and resulted in a marked reduction of rVAR2 binding. (lu.se)

Placental malaria is mediated by the protein VAR2CSA interacting with the placental glycosaminoglycan chondroitin sulfate, or CS. (asbmb.org)
Placental malaria infection is mediated by the binding of the malarial VAR2CSA protein to the placental glycosaminoglycan, chondroitin sulfate. (lu.se)

The arrangement and chemical composition of the glycans at the 22 N-glycosylation sites of SARS-CoV-2 spike protein and those at the sialoglycoprotein coating of RBCs allow exploration of specifics as to how virally induced RBC clumping may form. (bvsalud.org)
The in vitro and clinical testing of these possibilities can be sharpened by the incorporation of an existing anti-COVID-19 therapeutic that has been found in silico to competitively bind to multiple glycans on SARS-CoV-2 spike protein. (bvsalud.org)

Using a recombinant endonuclease-deficient Cas9-based gene promoter pulldown approach coupled with mass spectrometry, we found that MCUb is upregulated in the T2D heart due to loss of glucose homeostasis regulator nuclear receptor corepressor 2 repression, and chromatin immunoprecipitation assays identified peroxisome proliferator-activated receptor α as a mediator of MCUb gene expression in T2D cardiomyocytes. (diabetesjournals.org)
Our active Cas9 protein carries nuclear localization signal (NLS) design. (lifetein.com)

We guarantee that predesigned Alt-R CRISPR-Cas9 guide RNAs will provide successful editing at the target site, when delivered as a ribonucleoprotein complex as described in the Alt-R Protocols, using Alt-R CRISPR-Cas9 guide RNAs (crRNA:tracrRNA duplex or sgRNA) and either Alt-R S.p. (idtdna.com)
By evaluating splicing changes they have discovered novel players in disease pathogenesis that include noncoding RNAs and RNA binding proteins. (chop.edu)

The student will master diverse methodologies, such as human cell culture manipulations (cultivation, RNAi, CRISPR/Cas9, etc.), recombinant DNA preparation, protein expression and purification, high-throughput analyses and enzymatic assays. (ceitec.eu)

It is caused by mutations in the SMN1 gene, which encodes a protein called survival of motor neuron (SMN). (scienceboard.net)

The CRISPR/Cas9-mediated homologous recombination system with donor DNA oligonucleotides of the human sites of the corresponding gene sites was used to humanize the fourth and fifth exons. (eco-vector.com)

The combination of Cas9 plus guide RNA forms a ribonucleoprotein (RNP). (idtdna.com)
For CRISPR-Cas9 endonuclease to have activity, the crRNA must be combined with a "trans-activating crRNA" (tracrRNA) to activate the endonuclease and create a functional editing ribonucleoprotein (RNP) complex (Figure 1A). (idtdna.com)

These products are available: Human EGFRVIII Protein, Human EGFR/HER1 Protein, Cynomolgus FAP Protein, Cynomolgus FOLR2 Protein, Human FOLR4/Juno Protein, Human GUCY2C, Human Her2/ErbB2 Protein and more. (lifetein.com)

This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. (origene.com)
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization). (antibodies-online.com)
We found very high levels of expression of the central complement protein, C3, and complement inhibitor CD59 in human pancreatic islets. (lu.se)
we found that the expression of cartilage protein COMP is associated with metastases and a poor prognosis for patients with various types of solid cancers. (lu.se)
August 31, 2021 -- A new alternative splicing system called Xon can modulate levels of protein expression in gene therapy, much like a dimmer switch. (scienceboard.net)
Although Novartis has since abandoned development of LMI070 in favor of newer therapeutics for the treatment of SMA, clinical trial data from the SMA study indicated that the drug also decreased expression of huntingtin protein, an important therapeutic target for Huntington's disease. (scienceboard.net)

The newly developed oocyte shuttle protein contains a streptavidin moiety that tightly binds biotinylated DNA. (biologists.com)
Injected intravenously into adult Xenopus females, the protein-DNA complex is rapidly transported through the bloodstream and, within the ovary, the vitellogenin ligand present in the protein binds to the receptors at the surface of the oocytes. (biologists.com)
The encoded protein is secreted and binds hydroxyapatite with high affinity. (origene.com)
A hyperphosphorylated, ubiquitinated, and cleaved form of TDP43 known as pathologic TDP43 is the major disease protein in ubiquitin-positive, tau-, and alpha-synuclein-negative frontotemporal dementia (FLTD-U). TDP43 is not related to TRBP1, and RNA binding protein that binds HIV-1 TAR RNA sequences. (thermofisher.com)
Thus this paper describes, for the first time, an MT gene identified from macroalgae which is induced by copper exposure and whose encoded protein product binds cadmium and copper. (portlandpress.com)

The guide RNA forms a complex with Cas9 endonuclease to guide targeted cleavage of genomic DNA. (idtdna.com)

this information needs to be taken with caution but that nevertheless seems likely as the rumors have run in recent months of work of Chinese research on the modification of human genes by the tool Gentaur CRISPR cas9 . (genprice.com)
Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. (novusbio.com)
Recombinant Human IL18RAP protein(Phe 20 - Arg 356), fused with His tag and Avi tag, was expressed in HEK293. (creativebiomart.net)
We have developed a high-quality Laminin 521 product and Human Wnt Surrogate-Fc Fusion Protein that can be applied to scientific research and preclinical development. (lifetein.com)
Antigen seize enzyme-linked immunosorbent assay (ELISA) for quantitation of the p24 gag protein of human immunodeficiency virus type-1 (HIV-1) is presently the commonest technique used to reveal virus replication each in vivo and in vitro. (ncbcs.org)

Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). (novusbio.com)

It is a fantastic recognition of the work that people in our lab have been doing for many years: first on protein synthesis, antibiotics targeting it and antibiotic resistance mechanisms that counter the antibiotics - and more recently, on bacterial viruses, bacteriophages. (lu.se)

CRISPR/Cas9 targeting of these proteins showed a reduction in cell-surface chondroitin molecular weight, which reduced the binding to recombinant VAR2, or rVAR2. (asbmb.org)

Unlocking the Mysteries of the Immune System: new Roles of complement proteins in Diabetes and Cancer. (lu.se)

In this research, by using aptamer-conjugated gold nanoparticles (aptamer-AuNPs) and a modified glassy carbon electrode (GCE) with reduced graphene oxide (rGO) and Acropora-like gold (ALG) nanostructure, a sandwich-like system provided for sensitive detection of heat shock protein 70 kDa (HSP70), which applied as a functional biomarker in diagnosis/prognosis of COVID-19. (bvsalud.org)
The projects feature state-of-the-art methods for the investigation of cell biology, such as flow cytometry and confocal microscopy, protein interaction analyses using the proximity-ligation assay and Biacore, and genetic manipulation employing the Cas9/CRISPR system. (lu.se)

In collaboration with Australian BioResources, the lab pioneered the production of genetically modified mice using CRISPR/Cas9 technology. (garvan.org.au)

Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. (novusbio.com)
Gene therapy displacement of endogenous MCUb with a dominant-negative MCUb transgene (MCUb W246R/V251E ) in vivo rescued T2D cardiomyocytes from metabolic inflexibility and stimulated cardiac contractile function and adrenergic responsiveness by enhancing phospholamban phosphorylation via protein kinase A. We conclude that MCUb represents one newly discovered molecular effector at the interface of metabolism and cardiac function, and its repression improves the outcome of the chronically stressed diabetic heart. (diabetesjournals.org)
Molecular modeling studies were used to predict the effects of LDLR missense variants on protein structure. (bvsalud.org)
An S-peptide Fucus MT fusion protein expressed in Escherichia coli exhibited a relative molecular mass of ∼ 14 kDa. (portlandpress.com)

Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. (novusbio.com)
The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. (origene.com)
In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. (antibodies-online.com)
Knockout cell lysate achieved by CRISPR/Cas9. (abcam.com)
If you require a native form of the protein please use the live cell version - found here . (abcam.com)
The lab's research on childhood onset neurodegenerative diseases is focused on experiments to better understand the biochemistry and cell biology of proteins deficient in these disorders, and to develop small molecule- or gene therapy-based strategies for therapy. (chop.edu)
The truncated form is degraded within the cell almost immediately, but the small percentage of SMN2 mRNAs which are successfully translated into full-length SMN protein can lessen the severity of SMA (caused by the absence of a functional SMN1 gene). (scienceboard.net)

Indeed, CRISPR Cas9 is an extremely easy to use genetic manipulation tool. (genprice.com)

Only about 10% to 20% of SMN2 gene transcripts are spliced into mature mRNAs coding for full-length SMN, while the remainder result in the deletion of exon 7 to encode a truncated version of the protein called SMNΔ7. (scienceboard.net)

ELISA is a well-established method and still considered as the gold-standard technique when it comes to protein quantification, but there are tricks to improve your results. (thermofisher.com)

In conclusion, the p.C184Y, p.G373D, p.G549D and p.R814Q variants alter protein stability and intramolecular interactions, while p.V797L has a minimal impact on protein stability, and p.V827I has no significant intramolecular interactions. (bvsalud.org)

Many researchers have just lately used the prediction of protein secondary construction (native conformational states of amino acid residues) to check advances in predictive and machine studying know-how equivalent to Neural Web Deep Studying. (biomol-informatics.com)
A gene was identified (1229 bp) with a putative open reading frame (204 bp) encoding a 67-amino-acid protein exhibiting several characteristic features of MT proteins, including 16 cysteine residues (24%) and only one aromatic residue. (portlandpress.com)

LifeTein produced a series of immune checkpoint proteins with multiple labels like biotin, FITC and PE. (lifetein.com)

CoVs have four main structural proteins: spike(S), membrane (M), envelope (E), and nucleocapsid (N) proteins. (medchemexpress.com)

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7. (abcam.com)

CrispR/Cas9 approaches) in vivo to improve disease phenotypes in relevant animal models. (chop.edu)

Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. (antibodies-online.com)

Anatomy17

Organisms26

Diseases3

Chemicals and Drugs90

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes72

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care5

Nuclease13

Antibodies5

Plasmids2

CRISPR-Cas9 System4

Genome14

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Enzymes1

MRNA5

CrRNA and tracrRNA2

Chondroitin polymerization2

Streptococcus1

Sequence4

Cells14

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Spike Protein2

Nuclear2

RNAs2

RNAi1

Encodes1

Exons1

Ribonucleoprotein2

Products1

Expression6

Binds5

Genomic1

Human5

Receptors1

Bacterial1

RVAR21

Immune system1

System2

Mice1

Molecular4

Cell7

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Encode1

ELISA1

Stability1

Residues2

Biotin1

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Purification1