• Recombinant adenovirus type 5 (rAd) has been used as a vaccine platform against many infectious diseases and has been shown to be an effective vaccine vector. (mdpi.com)
  • Phase 1 safety and immunogenicity evaluation of a multiclade HIV-1 candidate vaccine delivered by a replication-defective recombinant adenovirus vector. (emmes.com)
  • Here, we report the safety, tolerability, and immunogenicity of a replication-defective recombinant adenovirus serotype 5 (rAd5) vector HIV-1 candidate vaccine. (emmes.com)
  • Adenovirus has been associated with both sporadic and epidemic disease and, with regard to infections among military recruits, who were routinely immunized against types 4 and 7 from 1971 until the cessation of vaccine production in 1996. (medscape.com)
  • A live oral vaccine against adenovirus types 4 and 7 was approved for use in this population by the US Food and Drug Administration (FDA) in 2011, and subsequent incidence of acute respiratory disease declined. (medscape.com)
  • The iNCOVACC is a recombinant replication-deficient adenovirus vectored vaccine with a pre-fusion stabilized spike protein. (livemint.com)
  • The ChAdOx1-S [recombinant] vaccine uses a DNA adenovirus vector to elicit antibodies to the SARS-CoV-2 spike protein. (who.int)
  • One live vaccine licensed for use in the United States (oral adenovirus vaccine) is not attenuated. (cdc.gov)
  • The vaccine consisted of live recombinant human rabies foci in skunks ( 8,9 ). (cdc.gov)
  • rVSV-ZEBOV is a live-attenuated recombinant viral vaccine. (msdmanuals.com)
  • Ad26.ZEBOV is a recombinant DNA vaccine based on adenovirus type 26 (Ad26). (msdmanuals.com)
  • MVA-BN-Filo is a recombinant DNA vaccine based on modified vaccinia Ankara (MVA). (msdmanuals.com)
  • Since wild-type adenovirus is associated with a wide range of illnesses and enlists a variety of immune responses, so recombinant replication deficiency virus has been an attractive vector for gene therapy [20]. (genemedi.com)
  • To date, there have been many different generations of adenovirus vectors, differing in the extent to which the genome from wild-type adenovirus is attenuated, among them, most studies involving adenovirus vectors utilize the simple E1-deleted, first-generation adenovirus vector. (genemedi.com)
  • Due to the induction of strong innate and adaptive immune responses and limited the duration of transgene expression of first-generation adenovirus vector, researchers have further developed the second-generation adenovirus vectors by deleting the E2 or E4 coding sequences, resulting in some beneficial effects of reducing the expression of viral proteins and vector-directed immune responses. (genemedi.com)
  • YKL-1, E1B 55 kDa-deleted recombinant adenovirus vector, capable of harboring a transgene casette of up to 4.9 kb, was newly constructed by reintroducing E1A and E1B 19 kDa into E1/E3-deleted adenoviral vector with a homologous recombination in E. coll. (elsevierpure.com)
  • It is our expectation that this recombinant vector can provide protection against IBD and highly virulent MD challenge, concluded Dr Lupiani. (thepoultrysite.com)
  • Dr Reddy said it was his expectation that this recombinant vector can provide protection against IBD and Marek's disease challenge. (thepoultrysite.com)
  • Of interest is the role of adenoviruses as viral vectors in vaccination and in gene therapy.For example, in viral vector vaccines against SARS-CoV2, the vector virus is used to deliver RNA encoding SARS-CoV2 spike protein into target cells. (medscape.com)
  • Recombinant, replication-deficient chimpanzee adenovirus vector encoding the SARS-CoV-2 Spike (S) glycoprotein. (medicines.org.uk)
  • In this manuscript, we have developed a recombinant human adenovirus as an expression vector that contains the gene for porcine interferon alpha. (usda.gov)
  • Department of Health, Wildlife Services, and Ohio local consisting of a live recombinant vaccinia vector, expressing health jurisdictions used print media, television, radio, and rabies virus glycoprotein (V-RG) (Raboral V-RG) ( 2,3 ), was the Internet to raise awareness and provide guidance to the distributed in other areas of Ohio. (cdc.gov)
  • Traditionally, recombinant adenovirus vectors used in gene delivery were prepared with a plasmid containing the transgene flanked by inverted terminal repeats (ITRs), co-transfected with packaging plasmid pAd-BHGlox(delta)E1, E3. (genemedi.com)
  • Recombinant human adenoviral vectors provide an efficient delivery system and have many advantages over other viral and non-viral delivery vehicles. (illinois.edu)
  • Adenovirus (Ad) vectors are among the most commonly used viral vectors in gene therapy clinical trials because they have the following advantages. (hindawi.com)
  • On the other hand, Ad vectors also have a number of disadvantages, including their low transduction efficiency in cells lacking coxsackievirus-adenovirus receptor (CAR) and their induction of innate immune responses. (hindawi.com)
  • Little is known of the T cell repertoire these recombinant vaccines induce. (biomedcentral.com)
  • T cells primed by these recombinant adenovirus vaccines expanded after PPRV challenge and probably contributed to protection. (biomedcentral.com)
  • Recombinant adenovirus vaccines should therefore aim at replicating the naturally occurring PPRV immunity. (biomedcentral.com)
  • The generic group of ChAdOx1-S [recombinant] vaccines includes AstraZeneca/AZD1222 and SII/Covishield vaccines. (who.int)
  • WHO SAGE interim recommendations on the AstraZeneca COVID-19 vaccines refer to a generic group of ChAdOx1-S [recombinant] vaccines against COVID-19. (who.int)
  • or fractional vaccines such as recombinant vaccines, produced through genetic technologies. (cdc.gov)
  • Infection of a panel of human melanoma cell lines by AdCMVMAGE-1, a novel recombinant adenovirus which incorporates the full-length MAGE-1 cDNA, was shown to induce production of high levels of MAGE-1 protein. (illinois.edu)
  • Genomic analyses of human adenoviruses unravel novel recombinant genotypes associated with severe infections in pediatric patients. (cdc.gov)
  • In this review article, we outline key characteristics of major gene therapy viruses-adenovirus, adeno-associated virus (AAV), and lentivirus-and summarize the mechanisms regulating important steps in the virus journey from binding at peripheral nerve terminals to nuclear delivery. (frontiersin.org)
  • ViVU aims to provide the research community with lentivirus, adenovirus (Ad) and adeno-associated virus (AAV) for basic and preclinical studies. (cnic.es)
  • Moreover, the long-term systemic delivery of a recombinant protein is an expensive, painful experience for patients and is cumbersome for medical staff. (biomedcentral.com)
  • The group led by him is engaged in work concerning the determination of the role of cellular tumor antigen p53 in glioma, regulating the protein, p73 in cervical cancer patients, using Adenovirus early region 1A, a tumor suppressor gene, the analytical study of BRCA1 gene in breast cancer and the analysis and regulation of SV2A, a synaptic vesicle protein. (wikipedia.org)
  • NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. (thermofisher.com)
  • Regulation of recombinant gamma-aminobutyric acid (GABA)(A) and GABA(C) receptors by protein kinase C. (nih.gov)
  • The silkworm larvae infected with the recombinant virus produced 3·6 mg of the fusion protein per larva and the infected BM-N cells produced 0·3 mg per ml of culture. (microbiologyresearch.org)
  • IGF II was released from the fusion protein produced by BM-N cells infected with the recombinant virus by CNBr treatment, purified by extraction with guanidine-HCl, column chromatography and HPLC and the correct amino-terminal amino acid sequence confirmed. (microbiologyresearch.org)
  • Adenovirus is often cultured from the pharynx and stool of asymptomatic children, and most adults have measurable titers of anti-adenovirus antibodies, implying prior infection. (medscape.com)
  • Recovery from adenovirus infection is associated with the development of serotype-specific neutralizing antibodies. (medscape.com)
  • Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. (thermofisher.com)
  • They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. (thermofisher.com)
  • Rabbits and mice vaccinated with the live recombinant virus produced antibodies that recognized 48K, 57K and 61K mol. (microbiologyresearch.org)
  • The genome of adenovirus is well known and can be modified with relative ease to induce lysis or cytotoxicity of a specified cell line without affecting others. (medscape.com)
  • It also exhaustively introduces the concrete mechanism of invading GC cells and the viral genome composition of adenovirus and herpes simplex virus type 1 (HSV-1). (frontiersin.org)
  • The adenovirus genome is a linear, double stranded DNA of approximately 36 Kb. (cnic.es)
  • To generate recombinant adenovirus, that can be safely used as a genetic tool, its genome has two genes deleted: E1 and E3. (cnic.es)
  • Adenoviruses are immunogenic and elicit strong innate and adaptive immune responses. (medscape.com)
  • Immunization strategies against this morbillivirus using recombinant adenoviruses expressing PPRV-F or -H proteins can protect PPRV-challenged animals and permit differentiation of infected from vaccinated animals. (biomedcentral.com)
  • Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. (gentaur.com)
  • Cells infected with this recombinant virus (gG-VAC) synthesized glycosylated proteins of 48K, 57K and 61K mol. (microbiologyresearch.org)
  • It was developed in partnership with Washington University, St. Louis, which had designed and developed the recombinant adenoviral vectored construct and evaluated in preclinical studies for efficacy. (livemint.com)
  • The first is lytic infection, which occurs when an adenovirus enters human epithelial cells and continues through an entire replication cycle, which results in cytolysis, cytokine production, and induction of host inflammatory response. (medscape.com)
  • Guinea pig primary peripheral lung epithelial cells (PLECs) transfected with adenovirus E1A (E1A-PLECs) were compared to guinea pig normal lung fibroblasts (NLFs) transfected with E1A (E1A-NLFs). (ersjournals.com)
  • Case-control studies have shown that the E1A DNA from group C adenoviruses is present in greater amounts in the lungs of patients with COPD than in controls with normal lung function matched for age, sex and smoking history 5 , and that the excess inflammation in the lungs of smokers with severe emphysema is associated with increased numbers of alveolar epithelial cells expressing E1A 6 . (ersjournals.com)
  • This information would enhance the understanding of recombination of HAdV-C, and might assist with effective prevention and control of respiratory adenovirus infection in Qinghai Province. (biomedcentral.com)
  • These primary cell cultures were often noted to spontaneously degenerate over time, and adenoviruses are now known to be a common cause of asymptomatic respiratory tract infection that produces in vitro cytolysis in these tissues. (medscape.com)
  • Upon infection with adenovirus, one of three different interactions with the cells may occur. (medscape.com)
  • Adenovirus infection activates akt1 and induces cell proliferation in pancreatic islets1. (nih.gov)
  • The present report concerns the role of adenovirus infection in inducing this transformation in an animal model of chronic obstructive pulmonary disease. (ersjournals.com)
  • These human observations are supported by experiments in guinea pigs, where, in the absence of actively replicating virus but the presence of E1A DNA, adenovirus infection resulted in excess lung inflammation following a single exposure to cigarette smoke 7 , and increased parenchymal and airway wall inflammation with greater emphysematous destruction after chronic exposure 8 . (ersjournals.com)
  • However, during periods of low influenza activity and outside of epidemics situations, the infection of other respiratory viruses e.g. rhinovirus, respiratory syncytial virus, parainfluenza and adenovirus can also present as Influenza-like Illness (ILI) which makes the clinical differentiation of influenza from other pathogens difficult. (who.int)
  • We also show that recombinant adenovirus vaccination induced T cell responses to the same epitopes, and led to memory T cell differentiation. (biomedcentral.com)
  • Together, our data emphasize the utility of adenoviruses as vaccination vehicles and highlight the potential efficacy of this approach for the treatment of melanoma. (illinois.edu)
  • Human adenoviruses (HAdVs) are prevalent in patients with respiratory infections, in which recombination has important implications for viral detection and pathogenicity. (biomedcentral.com)
  • In this study, we assayed the effect of exogenous mouse recombinant GDF-5 on cell proliferation of dental pulp cells and determined the expression levels of odontoblast differentiation marker genes in the cells of isolated mouse dental pulp tissues in the presence of GDF-5. (ispub.com)
  • In vitro studies demonstrate that exogenous recombinant Gal3 is internalised by neuronal cell lines and primary neurons where it interacts with endogenous αSyn fibrils. (lu.se)
  • Gene therapy using a recombinant adenovirus (Ad) encoding secretory human endostatin (Ad-Endo) has been demonstrated to be a promising antiangiogenesis and antitumor strategy of in animal models and clinical trials. (biomedcentral.com)
  • Currently, human adenoviruses (HAdVs) are classified into seven groups (HAdV-A to HAdV-G), including 52 serotypes and 90 genotypes [ 4 ]. (biomedcentral.com)
  • An extremely hardy virus, adenovirus is ubiquitous in human and animal populations, survives long periods outside a host, and is endemic throughout the year. (medscape.com)
  • Adenovirus infections do not normally cause hepatitis in healthy children suggesting that a completely new type of adenovirus might have been introduced into the human population. (bmj.com)
  • To further investigate these observations in vivo, we take advantage of WT and Gal3KO mice subjected to intranigral injection of adenovirus overexpressing human αSyn as a PD model. (lu.se)
  • Adenovirus is known to be oncogenic in rodents but not in humans. (medscape.com)
  • The resulting plasmid and the wild-type BmNPV genomic DNA were cotransfected into BM-N cells, and a recombinant virus was isolated by the limiting dilution method. (microbiologyresearch.org)
  • Adenovirus infections might cause the acute hepatitis in children observed. (bmj.com)
  • The following product was used in this experiment: BNIP3 Recombinant Polyclonal Antibody (8HCLC) from Thermo Fisher Scientific, catalog # 710728, RRID AB_2607982. (thermofisher.com)
  • While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. (thermofisher.com)
  • The MTT assay showed that recombinant adenovirus-mediated overexpression of KRT10 and PTEN enhanced the proliferation inhibition effect of cisplatin on C13K cells. (geneticsmr.com)
  • To investigate the effects of glucose-6-phosphatase catalytic subunit (G6PC) recombinant adenovirus on proliferation and cell cycle regulation of liver cancer cells . (bvsalud.org)
  • Here, we describe the characterization of an HAdV-1 recombinant (QH-1665/2018) isolated from an infant aged one month with influenza-like illness in Qinghai Province in 2018. (biomedcentral.com)
  • Once packaged into a E1-complementing cell line, such as QBI 293A cells, recombinant viral will be easily propagated. (genemedi.com)
  • E1 gene is implicated on replication of virus and is supplied by the adenovirus packaging cell line 293A. (cnic.es)
  • QH-1665/2018 caused adenovirus-like CPE of HEp-2 cells, and the cultures underwent three passages to obtain high-titer stocks. (biomedcentral.com)
  • Recombinant adenovirus-mediated overexpression of KRT10 and PTEN also increased the cisplatin-induced apoptosis rate of C13K cells. (geneticsmr.com)
  • The Recombination Detection Program (RDP) package Beta 4.100 was used for identification of recombinant sequences in default mode. (biomedcentral.com)
  • Adenovirus, a DNA virus, was first isolated in the 1950s in adenoid tissue-derived cell cultures, hence the name. (medscape.com)
  • Swine given one inoculation of this recombinant virus and then challenged with virulent FMDV one day postinoculation were completely protected from clinical signs of disease and viremia. (usda.gov)
  • The recombinant HVT-VP2 viruses were stable and showed continuous expression of VP2 even after several passages in cell culture. (thepoultrysite.com)
  • sarilumab, adenovirus types 4 and 7 live, oral. (medscape.com)
  • The current method of the recombinant adenovirus production is based on Adeasy and Ad.MAX™ system. (genemedi.com)
  • These data validate the use of recombinant adenovirus expressing PPRV genes as DIVA strategies to control this highly contagious disease. (biomedcentral.com)
  • In contrast, control animals inoculated with an adenovirus lacking the interferon gene developed typical signs of FMD. (usda.gov)
  • Evidence for the involvement of sFlt1 in the occurrence of PE was initially provided by an animal model in which gravid rats were infected with a recombinant adenovirus encoding sFlt1 and compared to animals infected with the empty control adenovirus. (hindawi.com)
  • Therefore, we investigated the effects of adenovirus-mediated PTEN or KRT10 overexpression on the cisplatin resistance of ovarian cancer in vivo . (geneticsmr.com)