• Atacicept is a recombinant fusion protein combined with the extracellular ligand binding portion of TACI. (drugbank.com)
  • Mammalian cells proteolytically release (shed) the extracellular domains of many cell-surface proteins. (embl.de)
  • C07K2319/10 - Fusion polypeptide containing a localisation/targetting motif containing a tag for extracellular membrane crossing, e.g. (google.com)
  • We designed a medium throughput approach to systematically test for interactions between these pneumococcal surface proteins and host proteins (extracellular matrix proteins, circulating proteins or immunity related proteins). (biomedcentral.com)
  • Prothrombin (coagulation factor II) is proteolytically cleaved to form thrombin in the clotting process. (wikipedia.org)
  • Thrombin in turn acts as a serine protease that converts soluble fibrinogen into insoluble strands of fibrin, as well as catalyzing many other coagulation-related reactions. (wikipedia.org)
  • Thrombin is produced by the enzymatic cleavage of two sites on prothrombin by activated Factor X (Xa). (wikipedia.org)
  • In the blood coagulation pathway, thrombin acts to convert factor XI to XIa, VIII to VIIIa, V to Va, fibrinogen to fibrin, and XIII to XIIIa. (wikipedia.org)
  • As part of its activity in the coagulation cascade, thrombin also promotes platelet activation and aggregation via activation of protease-activated receptors on the cell membrane of the platelet. (wikipedia.org)
  • Thrombin bound to thrombomodulin activates protein C, an inhibitor of the coagulation cascade. (wikipedia.org)
  • The activation of protein C is greatly enhanced following the binding of thrombin to thrombomodulin, an integral membrane protein expressed by endothelial cells. (wikipedia.org)
  • This increase in the thrombin burst occurs after direct rFVIIa activation of factors IX and X on the surface of activated platelets (even in the absence of factor VIII or IX). (medscape.com)
  • rFVIIa is able to activate factor X on phospholipid vesicles, activated platelets, or monocytes independent of TF, although the TF-independent generation of thrombin is much less efficient than the TF-dependent thrombin generation by rFVIIa. (medscape.com)
  • They found that the coagulation factor thrombin greatly amplifies the prothrombotic and proinflammatory function of endothelial cells, and they identified part of the molecular mechanism that is responsible for this effect. (versiti.org)
  • The blood coagulation and Protein C pathway. (wikidoc.org)
  • Far more not long ago, it was proven that Autodisplay, which can be defined since the surface show of the recombinant protein through the autotransporter secretion pathway, relies on a set of periplasmic chaperones in cluding a complicated of proteins which corresponds for the so called Bam machinery in E. coli. (pkainhibitors.com)
  • Functional analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis resulted in the differentially expressed proteins in the FM and OA groups are mostly involved in the coagulation and complement system-related pathways, serotonin pathways, dopamine pathways, glutamate pathways and acute phase reactions. (ncl.edu.tw)
  • ARGX-119 is a monoclonal antibody that targets and activates muscle-specific kinase (MuSK). (drugbank.com)
  • Matched Description: … ARGX-119 is a monoclonal antibody that targets and activates muscle-specific kinase (MuSK). (drugbank.com)
  • MM-111 is a novel bispecific antibody fusion protein that co-targets the the epidermal growth factor family receptor tyrosine kinases, ERBB2 and ERBB3. (drugbank.com)
  • S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. (assaygenie.com)
  • Is there a difference in inhibitor incidence with recombinant products? (postersessiononline.eu)
  • The scientists discovered that T cells, which play a vital role in the immune system and help trigger factor VIII inhibitor formation, rely on a specific set of other cells to move coagulation factor VIII to the regions of the spleen where T cells reside. (cdc.gov)
  • This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. (cusabio.com)
  • These benefits are based on the fusion of special Factor VIII sequences with four albumin-binding peptides shielding the active substance from degradation. (pharmiweb.com)
  • Biotest develops and markets immunoglobulins, coagulation factors and albumin based on human blood plasma. (pharmiweb.com)
  • The first report in 2007, on SAA-stimulated human coronary artery endothelial cells (HCAEC), exhibited a substantial and significantly higher induction of released IL-6 protein and mRNA levels as compared to HUVEC [ 17 ], as well as increased responsiveness to IL-1 β [ 18 ]. (hindawi.com)
  • Among the scientific community, it is now a well-recognized fact that activation of the blood coagulation system makes an important contribution to the body's defenses in a variety of conditions and diseases, in large part by regulating the functions of immune cells and of endothelial cells lining the inner surface of our blood vessels. (versiti.org)
  • Dr. Weiler's group investigates how activated blood coagulation factors alter the function of infected endothelial cells. (versiti.org)
  • Current work examines how activated protein C protects blood-forming stem cells in the bone marrow, how radiation alters the function of blood vessel endothelial cells, and how natural anticoagulant mechanisms alter this response. (versiti.org)
  • Disintegrins contain an RGD (Arg-Gly-Asp) or KGD (Lys-Gly-Asp) sequence motif that binds specifically to integrin IIb-IIIa receptors on the platelet surface, thereby blocking the binding of fibrinogen to the receptor-glycoprotein complex of activated platelets. (embl.de)
  • The rFVIIa seems to work in a TF-independent manner directly on factors IX and X on the phospholipid surface of activated platelets. (medscape.com)
  • conclude that residues 88 to 109 of the second EGF domain mediate binding to platelets and assembly of the factor X activating complex. (wikidoc.org)
  • computer simulations of proteolysis of marburg and ebola-zaire filovirus coded proteins to generate nonapeptides with motifs of known hla class i haplotypes and detection of antigenic domains in the viral glycoproteins. (liverpool.ac.uk)
  • Besides their function in the virus replication cycle, the viral glycoprotein, nucleoprotein, minor matrix protein and polymerase cofactor are viral determinants of pathogenicity, with evasion of the host innate and adaptive immune responses as the main mechanism. (biomedcentral.com)
  • Mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. (assaygenie.com)
  • During viral and target cell membrane fusion, the coiled coil regions (heptad repeats) assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain. (assaygenie.com)
  • The formation of this structure appears to drive apposition and subsequent fusion of viral and target cell membranes. (assaygenie.com)
  • Acts as a viral fusion peptide which is unmasked following S2 cleavage occurring upon virus endocytosis. (assaygenie.com)
  • Two (the Moderna vaccine and the Pfizer/BioNTech vaccine) are based on mRNA technology, whereas the other two (produced by Johnson & Johnson and AstraZeneca) are based on a double-stranded DNA recombinant viral vector. (theepochtimes.com)
  • The mRNA vaccines contain only the code for the SARS-CoV-2 envelope spike protein, whereas the DNA-based vaccines both contain an adenovirus viral vector that has been augmented with DNA that codes for the SARS-CoV-2 spike protein. (theepochtimes.com)
  • A recombinant vesicular stomatitis vaccine vector protected non-human primates in pre- and post-exposure challenge studies. (biomedcentral.com)
  • ZMapp), anti-sense phosphorodiamidate morpholino oligomers (PMO AVI-6002), lipid nanoparticle small interfering RNA (LNP-siRNA: TKM-Ebola), and an EBOV glycoprotein-based vaccine using live-attenuated recombinant vesicular stomatitis virus (rVSV-EBOGP) or a chimpanzee adenovirus (rChAd-EBOGP)-based vector. (biomedcentral.com)
  • A combination of DNA vaccination and a recombinant adenovirus serotype 5 boost resulted in cross-protective immunity in non-human primates. (biomedcentral.com)
  • But a disadvantage is that those who have been exposed to natural forms of the adenovirus have antibodies to the virus that will likely block the synthesis of the spike protein, and therefore not afford protection against SARS-CoV-2. (theepochtimes.com)
  • optimal dilutions of specific immunoglobulin (1:500, corresponding to protein concentration of 50 micrograms/ml) and conjugate were found. (liverpool.ac.uk)
  • This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. (cancerindex.org)
  • What does this gene/protein do? (cancerindex.org)
  • What pathways are this gene/protein implicaed in? (cancerindex.org)
  • CCDC88C-FLT3 gene fusion in CD34-positive haematopoietic stem and multilineage cells in myeloid/lymphoid neoplasm with eosinophilia. (u-tokyo-hemat.com)
  • The human ALK gene encodes a protein 1,620 amino acids long with a molecular weight of 180 kDa. (alksignaling.com)
  • The gene construct applied in Autodisplay Cabozantinib encodes a fusion protein comprised of an N terminal signal peptide derived from cholera toxin B subunit, a variable passenger domain along with the C terminal AIDA I autotransporter such as a linker to allow full surface accessibility of your passenger domain. (pkainhibitors.com)
  • Interactions were tested in a solid phase assay, which led to the identification of 23 protein-protein interactions among which 20 are new. (biomedcentral.com)
  • Include a diagnostic workup for hemorrhage, but never delay indicated coagulation correction pending diagnostic testing. (medscape.com)
  • After initiating coagulation therapy, perform early and aggressive imaging, even when there is a low suspicion for hemorrhage. (medscape.com)
  • This has become the case for treatment of hemorrhage with recombinant factor VIIa (rFVIIa). (medscape.com)
  • Although this division is useful for understanding in vitro laboratory coagulation tests, no such division occurs in vivo because the tissue factor VIIa complex is a potent activator of factor IX and factor X. (medscape.com)
  • Results from further animal models support a 4-fold extension of the half-life in blood circulation while maintaining the wild-type characteristics of activated Factor VIII with full in vivo functionality. (pharmiweb.com)
  • Specific enzymatic cleavages in vivo yield mature proteins. (assaygenie.com)
  • Therapeutic options for major bleeds include recombinant activated factor VIIa (rFVIIa), plasma-derived factor VII, fresh frozen plasma, and prothrombin complex concentrates. (medscape.com)
  • Recombinant factor VIIa (rFVIIa) has become available for treating people with hemophilia with inhibitors who experience bleeding or require surgery. (medscape.com)
  • The theoretical mechanism for the coagulation and hemostatic effects of rFVIIa are discussed. (medscape.com)
  • [ 13 ] rFVIIa has become available as a recombinant preparation and also has been used in nonhemophiliac conditions. (medscape.com)
  • In most developed countries with access to recombinant product, prophylaxis is primary (ie, therapy is started in patients as young as 1 y and continues into adolescence). (medscape.com)
  • Members of the discoidin (DS) domain family, which includes the C1 and C2 repeats of blood coagulation factors V and VIII, occur in a great variety of eukaryotic proteins, most of which have been implicated in cell-adhesion or developmental processes. (embl-heidelberg.de)
  • Type 1 repeats in thrombospondin-1 bind and activate TGF-beta. (embl.de)
  • The subgroup A proteins TSP-1 and -2 contain an N-terminal domain, a VWFC domain, three TSP1 repeats, three EGF-like domains, TSP3 repeats and a C-terminal domain. (embl.de)
  • Characterization of mutant forms of recombinant human properdin lacking single thrombospondin type I repeats. (embl.de)
  • Annexin A7 is a Ca 2+ - and phospholipid-binding protein, which was isolated as the agent that mediated aggregation of chromaffin granules and fusion of phospholipid membranes in the presence of Ca 2+ . (biomedcentral.com)
  • AnxA5 2D-network mediates E-cadherin mobility in the plasmalemma that triggers human trophoblasts aggregation and thereby cell fusion. (cusabio.com)
  • Preferential activation of microsomal diacylglycerol/protein kinase C signaling during glucose treatment (De Novo phospholipid synthesis) of rat adipocytes. (jci.org)
  • Glucose has been reported to increase the de novo synthesis of diacylglycerol (DAG) and translocate and activate protein kinase C (PKC) in rat adipocytes. (jci.org)
  • Interaction of the SARS-CoV-2 spike protein with angiotensin-converting enzyme 2 (ACE2) receptors on target the cell surface mediates SARS-CoV-2 entry into cells that is facilitated by transmembrane protease serine 2 (TMPRSS2) and lysosomal proteases. (springer.com)
  • Viperin is a type-I and -II interferon-inducible intracytoplasmic protein that mediates antiviral activity against several viruses. (alksignaling.com)
  • Coagulation has traditionally been considered to occur via extrinsic and intrinsic pathways. (medscape.com)
  • Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. (creativebiomart.net)
  • Several years ago, Dr. Weiler's group began collaborating with researchers from three different institutions to show that the key effector molecule of the natural protein C anticoagulant pathways, activated protein C, can protect mice against the injury caused by exposure to high doses of  radiation. (versiti.org)
  • Deficiency of vitamin K or administration of the anticoagulant warfarin inhibits the production of gamma-carboxyglutamic acid residues, slowing the activation of the coagulation cascade. (wikipedia.org)
  • Several structures of 'super active' mutants have been solved, [10] which reveal the nature of factor IX activation by other proteins in the clotting cascade. (wikidoc.org)
  • Inherited deficiency of factor VII (FVII), the crucial enzyme triggering blood coagulation, is the most common of the rare coagulation disorders transmitted in an autosomal recessive manner. (medscape.com)
  • In 1951, Alexander and colleagues identified factor VII as the key initiator of coagulation when they reported the first case of factor VII deficiency in a child and called it serum prothrombin conversion accelerator deficiency. (medscape.com)
  • Hemophilia B, or Christmas disease, is an inherited, recessive disorder that involves deficiency of functional coagulation factor IX (FIX) in plasma. (medscape.com)
  • Deficiency of this protein causes haemophilia B . It was discovered in 1952 after a young boy named Stephen Christmas was found to be lacking this exact factor, leading to haemophilia . (wikidoc.org)
  • Human recombinant apo-SAA was used to stimulate HCAEC at a final 1000 nM concentration for 24 hours. (hindawi.com)
  • Recombinant Human ANXA3 Protein 1-323 aa (full-length) with a 6xHis tag was expressed in E.coli. (creativebiomart.net)
  • We felt that this drug, recombinant human activated protein C, was used in an incorrect manner," he said. (versiti.org)
  • Blood coagulation affects a variety of diseases, including how the immune system responds to viruses, bacteria and injury. (versiti.org)
  • Dr. Weiler is also researching how the blood coagulation system, as part of the immune system, affects patients who develop sepsis, or blood poisoning. (versiti.org)
  • Factor VII is a vitamin K-dependent serine protease glycoprotein (also known as stable factor or proconvertin) with a pivotal role in hemostasis and coagulation. (medscape.com)
  • The activity of factor Xa is greatly enhanced by binding to activated Factor V (Va), termed the prothrombinase complex. (wikipedia.org)
  • Allergic reactions may occur with coagulation factor IX . (rxwiki.com)
  • At the site of injury, tissue factor (TF) and factor VIIa activate factors X and IX. (medscape.com)
  • [3] [4] When activated into factor IXa , in the presence of Ca 2+ , membrane phospholipids, and a Factor VIII cofactor, it hydrolyses one arginine - isoleucine bond in factor X to form factor Xa. (wikidoc.org)
  • [13] Recombinant factor IX is used to treat Christmas disease. (wikidoc.org)
  • It has been shown to interact with other proteins involved in the inflammatory response and can modulate the production of pro-inflammatory molecules. (creativebiomart.net)
  • In the Dictyostelium discoideum (Slime mold) cell adhesion protein discoidin, a related domain, named discoidin I-like domain, DLD, or DS, has been found which shares a common C-terminal region of about 110 amino acids with the FA58C domain, but whose N-terminal 40 amino acids are much less conserved. (embl-heidelberg.de)
  • The mRNA in these vaccines codes for the spike protein normally synthesized by the SARS-CoV-2 virus. (theepochtimes.com)
  • However, both the mRNA and the protein it produces have been changed from the original version in the virus with the intent to increase rate of production of the protein in an infected cell and the durability of both the mRNA and the spike protein it codes for. (theepochtimes.com)
  • Activation of blood coagulation and endothelial inflammation are hallmarks of respiratory infections with RNA viruses that contribute significantly to the morbidity and mortality of patients with severe disease, such as COVID-19. (versiti.org)
  • Disintegrin-like proteins are found in various species ranging from slime mold to humans. (embl.de)
  • the primary amino acid sequences of the proteins coded by marburg and ebola-zaire filoviruses were studied by computer programs to search for putative proteolytic cleavages which yield nonapeptides with motifs of binding to known hla class i haplotypes. (liverpool.ac.uk)
  • a few nonapeptides with motifs hla class i a24, b8, b27 and b35 were predicted in marburg virus proteins. (liverpool.ac.uk)
  • Availability of genome sequences facilitated the identification of pneumococcal surface proteins bearing characteristic motifs such as choline-binding proteins (Cbp) and peptidoglycan binding (LPXTG) proteins. (biomedcentral.com)
  • Pneumococcal surface proteins represent challenging candidates for the development of new therapeutic targets against the bacteria. (biomedcentral.com)
  • Blood coagulation is a series of reactions in which plasma zymogens are converted into active enzymes. (medscape.com)
  • were able to show the LipH chaperone of P. aeruginosa in Inhibitors,Modulators,Libraries an active state within the surface of E. coli through the use of the P. aeruginosa autotransporter protein EstA. (pkainhibitors.com)
  • described the show of ac tive P. aeruginosa and B. cepacia lipases about the surface of E. coli through co expression of lipase as well as the Lif protein within a single fusion protein. (pkainhibitors.com)
  • Autodisplay is based on AIDA I, the adhesin concerned in diffuse adherence in enteropathogenic E. coli, a naturally occurring autotransporter protein in E. coli. (pkainhibitors.com)
  • Most almost certainly, the linker along with the B barrel are accountable for that translocation with the passenger protein across the E. coli outer membrane. (pkainhibitors.com)
  • This enables the self driven dimerization or multimerization of subunits to lively or functional en zymes about the surface of E. coli, even in case they were expressed as monomers. (pkainhibitors.com)
  • Examples for this self driven dimerization sellckchem or multimerization of passsenger proteins on the cell surface of E. coli will be the active display of dimeric adrenodoxin, dimeric sorbit dehydrogenase, mul timeric nitrilase and dimeric prenyl transferase. (pkainhibitors.com)
  • The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. (assaygenie.com)
  • Just as in the case of antibiotic resistance, new strains evolve within an infected immune-compromised person's body that produce a version of the spike protein that no longer binds with the acquired antibodies. (theepochtimes.com)
  • Furthermore, through co-immunoprecipitation and laser confocal microscopic analysis, viperin was found to interact with the host protein hVAP-33. (alksignaling.com)
  • We conclude that whether peptidoglycan binding proteins do not appear to be major adhesins, most of the choline-binding proteins interact with host proteins (elastin and C reactive proteins are the major Cbp partners). (biomedcentral.com)
  • Blood coagulation factors V and VIII contain a C-terminal, twice repeated, domain of about 150 amino acids, which is called F5/8 type C, FA58C, or C1/C2- like domain. (embl-heidelberg.de)
  • We also identified CapG (a macrophage-capping protein) as a possible molecule that binds langerin by immunoprecipitation combined with a mass spectrometry analysis. (bvsalud.org)
  • in a mouse model, a molecularly engineered FIX molecule maintained protection against bleeding approximately three times longer, compared with recombinant FIX. (medscape.com)
  • We observed marked influence of fluvastatin on lowering cytokine production in SAA-activated HCAEC. (hindawi.com)
  • The coagulation of blood is maintained by cellular components and soluble plasma proteins. (medscape.com)
  • 32142651 ). Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes. (assaygenie.com)
  • A polypeptide substance comprising about one third of the total protein in mammalian organisms. (lookformedical.com)
  • It now appears clearly that cell-surface proteins participate in many stages of the colonization process and/or the disease transition. (biomedcentral.com)
  • Its principal role is to act as a messenger carrying instructions from DNA for controlling the synthesis of proteins. (rodefshalom613.org)
  • This process requires a transient intermediary called messenger RNA that carries the genetic information to the cell machinery responsible for protein synthesis. (rodefshalom613.org)
  • Throughout his career at Versiti Blood Research Institute (BRI), Senior Investigator Hartmut Weiler, PhD , has studied how the blood coagulation system and natural anticoagulant mechanisms affect the body's stress responses to challenges like pregnancy, injury and infections. (versiti.org)
  • He and his collaborators discovered a way to use a slightly altered version of the same drug that only activated protective mechanisms but lacked harmful side effects. (versiti.org)
  • This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. (creativebiomart.net)