Recognition base sequence. Medical search. Frequent questions

One could say the primary limitation to crop improvement is no longer, "can we make a precise sequence change in a precise location in eukaryotic genomic DNA? (frontiersin.org)
As the relationship between sequence variation and disease management becomes better understood, genomic medicine-the use of information from an individual's genome in the diagnosis and management of their condition-will become increasingly relevant to a broad range of health practitioners. (nature.com)
NEW YORK - Irvine, California-based Genomic Testing Cooperative (GTC) said on Tuesday it is collaborating with C2i Genomics to develop liquid biopsy tests for solid tumor staging and monitoring. (genomeweb.com)
We believe that broad whole-genome pattern recognition of genomic abnormalities is a reliable approach for cancer patient staging and monitoring," GTC CEO Maher Albitar added. (genomeweb.com)

Such sense--antisense peptide recognition has been observed in many systems, most often by detecting binding between immobilized and soluble interaction partners. (nih.gov)
Data obtained so far on sequence and solvent dependence of interaction support a hydrophobic-hydrophilic (amphipathic) model of peptide recognition. (nih.gov)
Understanding the mechanism of sense--antisense peptide recognition also may provide insights into mechanisms of native (sense) peptide and protein interactions and protein folding. (nih.gov)
Peptide-polyethylene oxide (PEO) conjugates, consisting of a previously identified specifically binding peptide sequence and polymer-block, are incorporated in the polymer composite material, composed of MgF2 nanoparticles particles and PEO. (hu-berlin.de)
Concept of bioinspired compatibilizer was expanded to the implementation of different peptide sequences. (hu-berlin.de)
Evaluation of secondary interactions revealed that the smaller the affinity for peptide-peptide interaction, the higher the availability of peptide sequences for an inorganic surface. (hu-berlin.de)
Efforts to mimic nature typically utilize amino acids and amino acid derivatives to form peptidomimetics having amide bonds.Presented here is a new class of synthetic sequence-defined polymers which do not use peptide bonds. (pnnl.gov)
This sequential reactivity provides advancement over prior methods used to form conventional sequence-defined peptide polymers because it provides completely new polymeric architectures, as compared to conventional peptide, peptoid, or nucleic acid polymers. (pnnl.gov)
MMP-13 was unable to bind to a linear peptide of the same sequence as II-44. (cam.ac.uk)

In bilaterian animals, the specificity of miRNA targeting is determined by sequence complementarity involving the seed. (iric.ca)
Improvement of the specificity of the molecular beacons was also achieved by the incorporation within their recognition sequence of a one-base mismatch. (diva-portal.org)
The recognition of collagen and triple-helical toolkit peptides by MMP-13: sequence specificity for binding and cleavage. (cam.ac.uk)
To determine the actual sequence specificity to SRP, a collection of mutants was constructed. (nature.com)

Here, using reporter assays, we deciphered the role of the base pairs formed between the non-seed region and target mRNA. (iric.ca)
Previous studies in flies, frogs and fish found pathways that act through specific mRNA sequences to recruit the cellular machinery and promote or inhibit mRNA decay. (harvard.edu)
Therefore, we do not know the rules that decode mRNA sequences into decay patterns. (harvard.edu)
To uncover the sequence-based rules of mRNA degradation, the authors developed UTR-Seq. (harvard.edu)
UTR-Seq tests thousands of sequences in parallel for their effect on mRNA stability and then builds computational models to decode the sequence-based rules of mRNA decay. (harvard.edu)
The resulting computational models established sequence-based rules that were able to predict the kinetics of mRNA decay during early zebrafish embryogenesis. (harvard.edu)

GTC will adapt and distribute New York-based C2i's tools, which uses AI-based pattern recognition of whole-genome sequencing of cell-free DNA, to its Co-op members after the partners complete technical and clinical validation. (genomeweb.com)
Whole genome sequencing helped detect this Listeria outbreak in a food source that would likely never have been identified without AMD methods. (cdc.gov)
AMD helped to support the infrastructure at CDC and in state and local health departments to move from a 20-year-old technology (PFGE) to state-of-the-art whole-genome sequencing. (cdc.gov)
In this study, we screened the whole genome of Aedes aegypti , the primary vector of several pathogenic viruses , and identified the proteins containing RNA recognition motif (RRM), the most abundant protein domain in eukaryotes . (bvsalud.org)

We present a discriminative part-based approach for human action recognition from video sequences using motion features. (sciweavers.org)

In many crops, genome engineering techniques have enabled researchers and breeders to take advantage of a vast knowledge base of plant physiology, pathology, and genetics. (frontiersin.org)
1 The ability to sequence an entire human exome or genome provides vastly more information of potential relevance to a person's current and future health than was possible previously. (nature.com)
When a HEG is integrated into its recognition sequence in the genome, its protein product acts to cleave its cognate site on the homologous chromosome and gene conversion or homologous recombination can result in a new copy of the HEG being inserted. (plos.org)
Rapid advances in human genome sequencing technologies have accelerated the integration of genomics into clinical practice. (cdc.gov)

By curating TA proteins with experimentally determined localizations and assessing hypotheses for recognition, we bioinformatically and experimentally verify that a hydrophobic face is the most accurate singular metric for separating ER and mitochondria-destined yeast TA proteins. (caltech.edu)
In contrast to the signal sequences of exported proteins, the bacterial SRP signal sequences are more hydrophobic and are mostly "uncleaved signal sequences" present in membrane proteins that remain in the final protein-chain as transmembrane anchor sequences. (nature.com)
The increase of the hydrophobicity in the signal sequences of E. coli presecretory proteins makes it possible to re-route the SecA dependent preproteins into the SRP targeting cycle 17 . (nature.com)
In silico identification, characterization, and expression analysis of RNA recognition motif (RRM) containing RNA-binding proteins in Aedes aegypti. (bvsalud.org)
The proteins were characterized based on their available annotations and the sequence similarity with Drosophila melanogaster . (bvsalud.org)

Large scale sequencing initiatives and discovery-based research projects address gaps in understanding the clinical significance of genetic variation. (nature.com)
However, there was a broad recognition that agricultural genetic resources - which cross international boundaries - required different solutions. (merid.org)
While genetic resources are not patentable, the inventions based on them may be. (merid.org)
Since these agreements were drafted, however, technology has drastically changed: today, genetic material can be digitally sequenced, stored in databases, and shared with the click of a button. (merid.org)
The work of the Section wide genotyping studies, as well as ants may be conferring genetic suscep- combines large population-based studies extensive sequencing work. (who.int)

Overall, our discovery improves the design of miRNA-guide sequences used to simultaneously downregulate the expression of multiple predetermined target genes. (iric.ca)
Sequencing of a 2.5-kb chromosomal Sma I- Pst I fragment containing the structural gene revealed an open reading frame encoding 537 amino acids, homologous to known pckA genes. (asm.org)
Burt proposed that such methods could be applied to engineer HEGs that recognise and cleave sequences within coding sequences of genes in insect genomes, with the subsequent invasion of these HEGs into a population leading to the inactivation of target genes and the subsequent decline in fitness of the targeted population [2] . (plos.org)
In a Novartis-sponsored study in the New England Journal of Medicine, researchers found that a CRISPR-Cas9-based treatment targeting promoters of genes encoding fetal hemoglobin could reduce disease symptoms. (genomeweb.com)
Expression analysis of the genes encoding RBPs using publicly available RNA sequencing data for different developmental time points of the mosquito life cycle starting from the ovary and eggs up to the adults revealed stage-specific expression with several genes preferentially expressed in early embryonic stages and blood -fed female ovaries . (bvsalud.org)

However, the specific residues upon which collagen recognition depends within and surrounding this locus have not been systematically mapped. (cam.ac.uk)
Within this sequence a Walker A motif is present at residues 30-38. (nature.com)
Furthermore, it is suggested that the binding of SRP to the signal sequence is promoted by the presence of basic amino acid residues through electrostatic interactions 18 . (nature.com)

Deep-sequencing allows for an in-depth characterization of sequence variation in complex populations. (biomedcentral.com)
Modern massive parallel sequencing (MPS) technologies, on the contrary, have the opportunity to allow in-depth characterization of sequence variation in more complex populations, including low-frequency viral strains. (biomedcentral.com)

9 Increasingly, there is recognition of the importance of implementation research to identify strategies which assist the adoption of genomics by clinicians. (nature.com)

Sequences with low solubility due to aggregation are less efficient in surface recognition. (hu-berlin.de)

On the one hand, we present two methods based on Recurrent Neural Networks, in particular, the Long Short-Term Memory Neural Network. (uab.es)

A virus variant model-based clustering method, ViVaMBC, is presented that explores quality scores and second best base calls for identifying and quantifying viral variants. (biomedcentral.com)
Standard genotyping such as direct PCR sequencing methods, however, only provides information on the most abundant sequence variants. (biomedcentral.com)
We gathered a series of 49 individuals from 33 families with RAD21 alterations [24 different intragenic sequence variants (2 recurrent), 7 unique microdeletions], including 24 hitherto unpublished cases. (springer.com)

Their application led to the successful design of artificial mRNAs with predetermined decay dynamics in early embryos: mutating or introducing specific sequences generated mRNAs with pre-determined decay profiles. (harvard.edu)
The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA. (hindawi.com)
miRNAs act as adaptors that employ a silencing complex to target mRNAs by selective base pairing, primarily in the 3′-untranslated region (3′-UTR). (hindawi.com)

They provided a slight increase in sensitivity, which however does not warrant the additional computational cost of running the offline base caller. (biomedcentral.com)

Once bound, the enzyme slides along the DNA, and upon encountering a second complex, cleaves downstream of the recognition site, typically 25-28 bases away. (neb.com)
Biochemical assay for enzyme activity and gene sequencing is the method of definitive diagnosis. (medscape.com)

Mismatch bases can often be corrected by the second best base, i.e. the base with the second highest intensity in the quadruplet. (biomedcentral.com)

Nonetheless, the mechanistic understanding of this type of molecular recognition remains incomplete. (nih.gov)
Nature uses sequence-defined polymers such as polypeptides to create biomaterials, encode information, perform biocatalysis, participate in molecular recognition, and shuttle species across membranes. (pnnl.gov)

Like individual Velcro hooks, each nucleotide can base pair with one identical copy of an amplified template, increasing the overall strength of the interaction. (acs.org)
Fortunately, base calls are complemented with quality scores which are derived from a quadruplet of intensities, one channel for each nucleotide type for Illumina sequencing. (biomedcentral.com)

These sequences differ only in their leader sequence and therefore lead to the same mature protein. (beckman.com)
During its biogenesis KdpD binds to the signal recognition particle (SRP) of Escherichia coli that consists of a 48-kDa protein Ffh and a 4.5S RNA. (nature.com)

AMD-funded DNA sequencing tools helped public health laboratory and disease detectives solve a 2016 E. coli outbreak linked to flour . (cdc.gov)
While this was not the first outbreak suspected to be related to E. coli contamination in flour, DNA sequencing in concert with epidemiologic investigation, allowed us to make the link for the first time. (cdc.gov)

Nigéria, en décembre 2022 et publie depuis lors des rapports mensuels. (who.int)

It achieves an 89\% recognition rate in a set of 333 sequences from the Cohn-Kanade data base. (upm.es)
T1 , T2 , and proton density were quantified directly from the undersampled data by the pattern recognition algorithm. (nih.gov)
Although the use of second best base calls appeared very promising in our data exploration phase, their utility was limited. (biomedcentral.com)
Moreover, the incorporation of quality scores is most appropriate for Illumina sequencing data. (biomedcentral.com)
Here we present such an approach based on multivariate pattern analysis and apply it to simulated and experimental single-molecule charge transport data. (imperial.ac.uk)
GTC will sequence samples and help with technical and clinical validation, while C2I will be responsible for artificial intelligence-based analysis of data, recruitment of samples, and various clinical trial activities. (genomeweb.com)
GHOST is a cloud-based system that integrates NGS data with bioinformatics and information technologies. (cdc.gov)
This data can be translated into evidence-based information for policy-makers that can. (who.int)

The current concept is based on the sequence specific recognition of the inorganic surface by peptides. (hu-berlin.de)

citation needed] While a postdoctoral fellow with Hamilton O. Smith at the Johns Hopkins University School of Medicine during 1969-70, Kelly determined the DNA sequences recognized by type II restriction enzymes, which subsequently became major tools in recombinant DNA research. (wikipedia.org)

Because they bind so tightly, Element says avidites work at 100-fold lower concentrations than typical NGS detection bases. (acs.org)
Successful commercial systems based on visual recognition range from entertainment to serious scientific research: face detection and recognition on personal devices, social media. (ualberta.ca)
However, one of the challenges in the detection of low-frequency viral strains concerns the errors introduced during the sequencing process. (biomedcentral.com)
Besides the image descriptors, ‎utilizing the object-based information provides robust ‎and reliable detection and recognition. (actapress.com)
The overall ‎acquisition system and proposed technique overcome the ‎conventional illumination, disorientation and scaling ‎problems in detection and recognition process. (actapress.com)

This unique lymphocyte population restricted by the CD1d molecule recognition has been identified as the natural killer T (NKT) cells. (beckman.com)

In this dissertation, we have investigated different methods for recognizing a single staff section (e.g. scores for violin, flute, etc.), much in the same way as most text recognition research focuses on recognizing words appearing in a given line image. (uab.es)
These methods are based in two different methodologies. (uab.es)
We have explored three different methods: (a) a graphic tree-structure representation, Dendrograms, that joins, at each level, its primitives following a set of rules, (b) the incorporation of Language Models to model the probability of a sequence of tokens, and (c) graph neural networks to analyze the music scores to avoid meaningless relationships between music primitives. (uab.es)
Based on the image sequences methods for recognition, reconstruction and motion analyses are developed to be applied in for instance IT, medical technology, robotics, meteorology. (lu.se)
Comparison of Regression and Machine Learning Methods in Depression Forecasting Among Home-Based Elderly Chinese: A Community Based Study. (cdc.gov)

We used molecular modeling to reveal that this mechanism corresponds to the formation of base pairs mediated by ordered motions of the miRNA-induced silencing complex. (iric.ca)
Homing endonucleases differ functionally from the more well-known restriction endonucleases in that they possess longer recognition sequences of 18-22 base pairs in length. (plos.org)

In this "SRP pathway", an open question is how a signal sequence is recognized since no consensus motif exists and is relevant for SRP binding. (nature.com)

Through AMD funding, networks of regional and state laboratories are applying next-generation sequencing to protect Americans from many infectious disease threats including Antibiotic Resistance (AR), foodborne disease (PulseNet), tuberculosis (TB), and influenza (flu). (cdc.gov)

Three signals explained late-onset degradation: miR-430 binding sites, AU-rich sequences and recognition sites for members of the Pumilio family. (harvard.edu)
With cross-linking studies, it could be shown that the hydrophobicity of the signal sequence is crucial for SRP binding, since lowering the hydrophobicity resulted in less efficient cross-linking 15 , 16 . (nature.com)

Type III restriction enzymes are a group of endonucleases that recognize a non-pallindromic sequence, comprising two inversely oriented sites. (neb.com)

We use an efficient appearance-based face tracker to locate the face in the image sequence and estimate the deformation of its non-rigid components. (upm.es)
We introduce a probabilistic procedure based on a nearest-neighbour approach to combine the information provided by the incoming image sequence with the prior information stored in the expression manifold in order to compute a posterior probability associated to a facial expression. (upm.es)
Over the last several years, a new area of research called deep learning has taken the machine learning community by storm, delivering very promising results in all areas of speech and image recognition. (getfreeebooks.com)
Similar to hCRF for object recognition, we model a human action by a flexible constellation of parts conditioned on image observations. (sciweavers.org)
Document analysis is the subject that deals with the extraction and processing of documents through image and pattern recognition. (uab.es)

We introduce a system that processes a sequence of images of a front-facing human face and recognises a set of facial expressions. (upm.es)
We adopt a model-based approach for facial expression recognition. (upm.es)
Predicting neuropsychiatric symptoms of persons with dementia in a day care center using a facial expression recognition system. (cdc.gov)

Type III enzymes recognize a 5-6 base, non-palindromic sequence, and require 2 inversely oriented recognition sites for cleavage. (neb.com)

The ease of fabrication, favourable wetting properties and low capacitance are some of the inherent advantages, for example compared to more conventional, silicon-based nanopore chips. (imperial.ac.uk)

Our experimental results show that our model is comparable to other state-of-the-art approaches in action recognition. (sciweavers.org)
Because currently deployed approaches appear to have been ineffective in controlling some arthropods, genetically-based approaches have been increasingly investigated as an alternative route to the control or eradication of arthropod threats [1] . (plos.org)

A better understanding of the type and geographic distribution of Borrelia species infecting humans will improve clinical recognition of disease, laboratory diagnosis, and prevention. (cdc.gov)

This work provides context for previous observations and opens the door for more detailed mechanistic experiments to determine the molecular factors driving this recognition. (caltech.edu)

This knowledge base is far from complete for all useful traits in all crops, with the majority of studies and commercial applications focusing on model species or widely grown, annual crops. (frontiersin.org)

The diagnosis may be suspected based on the patient history, physical examination, and laboratory findings. (medscape.com)

Dosimetric impact of deep learning-based CT auto-segmentation on radiation therapy treatment planning for prostate cancer. (cdc.gov)
Fully automated radiomic screening pipeline for osteoporosis and abnormal bone density with a deep learning-based segmentation using a short lumbar mDixon sequence. (cdc.gov)

The deterministic aspect of the grammar allowed us to predict precisely which points in the sequence should be subject to anticipation. (philpapers.org)

The sequence features that define efficient and specific hAGO2-dependent miRNA silencing guides. (iric.ca)
Based on miRBase version 21 released in June 2014 ( http://www.mirbase.org/ ), there are 1,881 miRNA precursors and 2,588 mature miRNAs in humans. (hindawi.com)

The strength of the approach lies in modeling the error probabilities based on the quality scores. (biomedcentral.com)
We also observed that the fraction of targets undergoing homing was temperature-sensitive, falling nearly four-fold when the temperature was lowered to 18°C. Taken together, this study demonstrates how a few simple measures can lead to substantial improvements in the HEG-based gene drive strategy and reinforce the idea that the HEG approach may be widely applicable to a variety of insect control programs. (plos.org)
Through AMD investments, CDC hepatitis experts developed a custom NGS approach for sequencing hepatitis C virus (HCV) and launched the Global Hepatitis Outbreak and Surveillance Technology (GHOST). (cdc.gov)

Primer extension analysis identified a transcriptional start site 72 bp upstream of the pckA initiation codon and an upstream sequence similar to ς 70 promoters. (asm.org)

In recognition of these differences, a separate set of guidelines will address pediatric-specific issues related to antiretroviral therapy. (cdc.gov)

Anatomy10

Organisms8

Diseases3

Chemicals and Drugs96

Analytical, Diagnostic and Therapeutic Techniques and Equipment37

Psychiatry and Psychology17

Phenomena and Processes85

Disciplines and Occupations3

Information Science13

Health Care3

Genomic4

Peptide9

Specificity4

MRNA6

Whole-genome4

Video sequences1

Genome4

Proteins5

Genetic5

Genes5

Residues3

Characterization2

Genomics1

Solubility1

Recurrent Neural N1

Variants3

MRNAs3

Computational1

Enzyme2

Mismatch1

Molecular recognition2

Nucleotide2

Protein2

Coli2

20221

Data8

Peptides1

Citation1

Detection5

Molecule1

Methods5

Pairs2

Motif1

Influenza1

Binding2

Restriction1

Image5

Facial3

Cleavage1

Inherent1

Approaches2

Clinical1

Mechanistic1

Species1

Laboratory1

Segmentation2

Predict1

MiRNA2

Approach3

Promoters1

Specific1