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  • impaired NK cell function
  • With age, the incidence of infectious diseases and malignancy rises dramatically, suggesting that impaired NK cell function might contribute to disease in these individuals. (ox.ac.uk)
  • inhibition
  • The role of KLRG1 in ageing is not known, and the mechanism of KLRG1-induced inhibition of NK cell function is not fully understood. (ox.ac.uk)
  • Antigen
  • These experiments show that the activating receptor Ly-49D specifically interacts with the MHC I antigen, H-2Dd, demonstrating the existence of alloactivating receptors on murine NK cells. (nih.gov)
  • HLA-E-dependent presentation of Mtb-derived antigen to human CD8+ T cells. (nih.gov)
  • In this report, three independent methods are used to demonstrate the ability of these cells to recognize Mtb-derived antigen in the context of the monomorphic HLA-E molecule.This is the first demonstration of the ability of HLA-E to present pathogen-derived antigen.Further definition of the HLA-E specific response may aid development of an effective vaccine against tuberculosis. (nih.gov)
  • In this report, three independent methods are used to demonstrate the ability of these cells to recognize Mtb-derived antigen in the context of the monomorphic HLA-E molecule. (nih.gov)
  • We examined the effectiveness of cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) blockade, alone or in combination with a granulocyte/macrophage colony-stimulating factor (GM-CSF)-expressing tumor cell vaccine, on rejection of the highly tumorigenic, poorly immunogenic murine melanoma B16-BL6. (nih.gov)
  • The earliest contact between antigen and the innate immune system is thought to direct the subsequent antigen-specific T cell response. (nih.gov)
  • The disease enhancement was associated with augmentation of T cell proliferation and production of Th1 cytokines in response to MOG35-55.We further showed that NK cells inhibit T cell proliferation triggered by antigen or cytokine stimulation.Taken together, we conclude that NK cells are an important regulator for EAE in both induction and effector phases. (nih.gov)
  • We further showed that NK cells inhibit T cell proliferation triggered by antigen or cytokine stimulation. (nih.gov)
  • Effect of NK cell deletion on antigen-induced proliferation of T line cells. (nih.gov)
  • To obtain insights into the mechanism of NK cell-mediated regulation, we conducted in vitro experiments, assaying antigen-induced proliferation of ZB-1 cells in the presence of irradiated spleen cells as APCs. (nih.gov)
  • The experiment showed that antigen-induced ZB-1 cell proliferation is enhanced when spleen cells were derived from NK-deleted mice. (nih.gov)
  • We obtained similar results not only with spleen cells from wild-type B6 mice, but also from β2m−/− mice (Fig. 9, A and B). These indicate that irradiated, spleen NK cells would inhibit antigen-induced Th1 proliferation. (nih.gov)
  • In contrast to NKT cells, NK cells do not express T-cell antigen receptors (TCR) or pan T marker CD3 or surface immunoglobulins (Ig) B cell receptors, but they usually express the surface markers CD16 (FcγRIII) and CD56 in humans, NK1.1 or NK1.2 in C57BL/6 mice. (wikipedia.org)
  • NK cells also play a role in the adaptive immune response: numerous experiments have demonstrated their ability to readily adjust to the immediate environment and formulate antigen-specific immunological memory, fundamental for responding to secondary infections with the same antigen. (wikipedia.org)
  • tumor
  • Defective NK cell activity was shown to increase susceptibility for viral infections and reduce tumor immune-surveillance. (ox.ac.uk)
  • Notably, heterogeneous expression of NKG2DLs was found in melanoma and CRC cells, with a decrease of these molecules along with tumor progression. (mendeley.com)
  • Tumor rejection was dependent on CD8(+) and NK1.1(+) cells but occurred irrespective of the presence of CD4(+) T cells. (nih.gov)
  • This study shows that CTLA-4 blockade provides a powerful tool to enhance T cell activation and memory against a poorly immunogenic spontaneous murine tumor and that this may involve recruitment of autoreactive T cells. (nih.gov)
  • RAE-1 is overexpressed in tumor cell lines and its expression is induced after viral infection and genotoxic stress. (nih.gov)
  • We attempted previously to characterize several variables that may affect the relationship between tumor and host immune cells but, taken one at the time, none yielded a convincing explanation. (biomedcentral.com)
  • In addition, the transcriptional profile of metastatic melanoma was compared with that of primary renal cell cancers (RCC) identifying several genes co-coordinately expressed by the two tumor types. (biomedcentral.com)
  • NK and gammadelta T cells can eliminate tumor cells in many experimental models, but their effect on the development of tumors caused by virus infections in vivo is not known. (umassmed.edu)
  • Polyomavirus (PyV) induces tumors in neonatally infected mice of susceptible strains and in adult mice with certain immune deficiencies, and CD8+ alphabeta T cells are regarded as the main effectors in anti-tumor immunity. (umassmed.edu)
  • Here we report that adult TCRbeta knockout (KO) mice that lack alphabeta but have gammadelta T cells remain tumor-free after PyV infection, whereas TCRbeta x delta KO mice that lack all T cells develop tumors. (umassmed.edu)
  • The protein kinase C-θ (PKCθ), which is essential for T cell function and survival, is also required for efficient anti-tumor immune surveillance. (frontiersin.org)
  • Natural killer (NK) cells, which express PKCθ, play a prominent role in this process, mainly by elimination of tumor cells with reduced or absent major histocompatibility complex class-I (MHC-I) expression. (frontiersin.org)
  • This justifies the increased interest of the use of activated NK cells in anti-tumor immunotherapy in the clinic. (frontiersin.org)
  • Recent data offer some clues on the mechanism that could explain the important role of PKCθ in NK cell-mediated anti-tumor immune surveillance: some studies show that PKCθ is implicated in signal transduction and anti-tumoral activity of NK cells elicited by interleukin (IL)-12 or IL-15, while others show that it is implicated in NK cell functional activation mediated by certain killer-activating receptors. (frontiersin.org)
  • The 14 amino acid sequence (aa 450-463 ) TKDNNLLGRFELSG (TKD) of heat shock protein 70 (Hsp70) was identified as a tumor-selective recognition structure for natural killer (NK) cells. (aacrjournals.org)
  • H-60 and Rae1 are structurally similar to MHC glycoproteins class I and their expression is increased in tumor cells. (wikipedia.org)
  • Natural killer (NK) cells are a type of lymphocyte cell involved in the innate immune system's response to viral infection and tumor transformation of host cells. (wikipedia.org)
  • Because natural killer cells target virally infected host cells and tumor cells, inhibitory KIR receptors are important in facilitating self-tolerance. (wikipedia.org)
  • NK cells provide rapid responses to viral-infected cells, acting at around 3 days after infection, and respond to tumor formation. (wikipedia.org)
  • Mice
  • Natural killer cells determine development of allergen-induced eosinophilic airway inflammation in mice. (nih.gov)
  • Moreover, systemic allergen-specific immunoglobulin (Ig)E and IgG2a levels and the number of IL-4 and interferon gamma-producing splenic cells were diminished in mice depleted of NK1.1(+) cells before the priming regime. (nih.gov)
  • CD1d1 mutant mice, deficient in NKT cells but with normal NK cells, developed lung tissue eosinophilia and allergen-specific IgE levels not different from those observed in wild-type mice. (nih.gov)
  • Mice deficient in gamma/delta T cells showed a mild attenuation of lung tissue eosinophilia in this model. (nih.gov)
  • Corresponding mice depleted of NK1.1+ cells exhibited a few scattered eosinophilic infiltrates only (b), or a complete absence of pulmonary inflammation (not shown). (nih.gov)
  • In contrast, corresponding mice depleted of NK1.1+ cells (groups 8 and 10) showed a clearly inhibited eosinophilia in lung tissue (Fig. 1, a and b). (nih.gov)
  • H) Number of BrdU positive cells in basal and uppermost two granular layers per cm epidermis of mice treated three times with TPA and injected with BrdU 24 hr before harvesting. (nih.gov)
  • When mice were deprived of NK cells by antibody treatment before immunization, they developed a more serious form of EAE associated with relapse. (nih.gov)
  • Aggravation of EAE by NK cell deletion was also seen in beta 2-microglobulin-/- (beta 2m-/-) mice, indicating that NK cells can play a regulatory role in a manner independent of CD8+ T cells or NK1.1+ T cells (NK-T cells). (nih.gov)
  • ZB-1 T line cells (4 × 104 cells/well) were stimulated with MOG35-55 in the presence of x irradiated spleen cells (8 × 105 cells/well) from wild-type B6 (A) or β2m−/− mice (B). In each experiment, spleen cells from mice pretreated with control mAb (control) and those pretreated with anti-NK1.1 mAb (anti-NK1.1) were compared in their accessory function. (nih.gov)
  • First, spleen cells from NK cell-deleted mice were compared with those from control mice as accessory cells for ZB-1 line cells. (nih.gov)
  • In addition, E26 mice, which lack NK and T cells, develop the tumors earlier than TCRbeta x delta KO mice. (umassmed.edu)
  • In mice, it is expressed by NK cells, NK1.1+ T cells, γδ T cells, activated CD8+ αβ T cells and activated macrophages. (wikipedia.org)
  • NK1.1
  • We hypothesized that cells of the innate immune system, such as natural killer (NK) cells, NK1.1(+) T cells (NKT cells), and gamma/delta T cells, may regulate the development of allergic airway disease. (nih.gov)
  • We demonstrate here that depletion of NK1.1(+) cells (NK cells and NKT cells) before immunization inhibits pulmonary eosinophil and CD3(+) T cell infiltration as well as increased levels of interleukin (IL)-4, IL-5, and IL-12 in bronchoalveolar lavage fluid in a murine model of allergic asthma. (nih.gov)
  • Depletion of NK1.1(+) cells during the challenge period only did not influence pulmonary eosinophilic inflammation. (nih.gov)
  • Effect of depleting NK1.1+ cells before immunization on the development of eosinophilic pulmonary inflammation. (nih.gov)
  • Numbers in the upper right hand corner correspond to percentage of NK1.1+ cells that are tetramer positive. (nih.gov)
  • lymphocyte
  • To obtain an NK cell-enriched lymphocyte population, PBMCs were depleted of plastic-adherent cells and subsequently incubated with anti-CD3 (JT3A) mAb for 30 min at 4°C followed by treatment with goat anti-mouse-coated Dynabeads (Dynal, Oslo, Norway) for 30 min at 4°C. The resulting CD3− lymphocyte populations contained ∼1% CD3+ cells, 20% HLA-DR+ cells, and 80% CD56+ cells. (nih.gov)
  • This role is especially important because harmful cells that are missing MHC I markers cannot be detected and destroyed by other immune cells, such as T lymphocyte cells. (wikipedia.org)
  • NK Cells are not a subset of the T lymphocyte family. (wikipedia.org)
  • C57BL
  • We then analyzed all Rae-1 transcripts possibly generated by these promoters in C57BL/6 cell lines and tissues using RT-PCR and agarose gel analysis. (nih.gov)
  • novel
  • BAB281 identified a novel NK cell-specific surface molecule of 46 kD (p46) that is expressed by all resting or activated NK cells. (nih.gov)
  • binds
  • As previously reported 23, tetramers generated from Qa-1b folded with Qdm peptide bind to 40-50% of splenic NK cells (Fig. 5 A). Staining with tetramers containing the Dk leader sequence, which contains Val instead of Ala at P3, was somewhat reduced compared with Qdm, yet a substantial fraction of NK cells clearly binds this reagent. (nih.gov)
  • peripheral
  • p46-mediated triggering of cytolytic activity in fresh peripheral blood NK cells. (nih.gov)
  • Analysis of IL-17 and IL-6 serum concentrations and peripheral CD4+ T cell phenotype.IL-17 (panel A) and IL-6 (panel B) serum levels were measured in 10 active CAPS patients, 20 active SoJIA patients and 20 healthy controls by ELISA. (nih.gov)
  • dendritic
  • T helper 17 cells (T(H)-17) represent a lineage of effector T cells critical in host defence and autoimmunity.Secretion of IL-1β, IL-23 and IL-6 by monocyte derived dendritic cells (MoDCs), were quantified by ELISA assay.These findings further support the central role of IL-1β in the differentiation of T(H)17 in human inflammatory conditions. (nih.gov)
  • Secretion of IL-1β, IL-23 and IL-6 by monocyte derived dendritic cells (MoDCs), were quantified by ELISA assay. (nih.gov)
  • CLEC7A is expressed by macrophages, neutrophils and dendritic cells. (wikipedia.org)