Loading...
  • Pathways
  • These results suggest that pathways involving Eph-ephrin signalling may be important in the progression of colon cancer and that therapies that target this receptor may find application in anti-cancer treatments. (biomedcentral.com)
  • Here we introduce phospho-dependent proteolysis targeting chimeras (phosphoPROTACs), a method to couple the conditional degradation of targeted proteins to the activation state of particular kinase-signaling pathways. (nih.gov)
  • We demonstrate the ability of these phosphoPROTACs to suppress the short- and long-term effects of their respective activating receptor tyrosine kinase pathways both in vitro and in vivo. (nih.gov)
  • signal transduction
  • which has a distinct structural character, that is the presence of the second src homology region 2 (SH2) instead of SH3, from other members of this group of PTK.Our efforts have in large part focused on the relationship between Syk in the context of surface receptor-initiated signal transduction in B cell and platelet because of their abundant localization. (nii.ac.jp)
  • By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. (biomol.com)
  • transmembrane proteins
  • Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. (biomol.com)
  • epithelial
  • Using immunohistochemistry and Western analysis techniques with an EphB4-specific antibody, we also show that this receptor is expressed in the epithelial cells of the tumour tissue and either not at all, or in only low levels, in the normal tissue. (biomedcentral.com)
  • binds
  • It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. (biomol.com)
  • Antibodies
  • Check out links to articles that cite our custom service antibodies, peptides, and proteins in major peer-reviewed journals, organized by research category. (abgent.com)
  • similarity
  • The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. (biomol.com)
  • stimulation
  • Abnormal expression of HER2 protein is thought to result in excess growth stimulation, converting HER2 into an oncogene . (ganfyd.org)
  • Furthermore, stimulation of unrelated growth factor receptors does not induce target protein knockdown. (nih.gov)
  • UniProt
  • p>When browsing through different UniProt proteins, you can use the 'basket' to save them, so that you can back to find or analyse them later. (uniprot.org)
  • sequence
  • Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed. (uniprot.org)
  • By varying the autophosphorylation sequence of a phosphoPROTAC, it is conceivable that other receptor tyrosine kinase/effector pairings could be similarly exploited to achieve other biological effects. (nih.gov)
  • target protein
  • A and B ) Schematic diagram of conditional posttranslational target protein knockdown by a phosphoPROTAC. (nih.gov)
  • A ) In the absence of an activated RTK, the phosphoPROTAC is not phosphorylated and fails to bind to the target protein, sparing it from degradation. (nih.gov)
  • Creating chemical diversity to target protein kinases. (ebi.ac.uk)
  • domain
  • B ) Upon activation of the RTK, the phosphoPROTAC is phosphorylated, creating a binding site for the SH2- or PTB-domain-containing effector protein and its subsequent recruitment for ubiquitination by VHL and proteasomal degradation. (nih.gov)
  • This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain. (biomol.com)
  • antibody
  • HER2 can be blocked using a synthetic humanised monoclonal antibody called trastuzumab , although the mechanisms of action may be more complex than just receptor antagonism. (ganfyd.org)
  • roles
  • To dissect the functional roles of these two types of kinase in BCR signaling, Syk-deficient cell and Lyn-deficient cell lines were established. (nii.ac.jp)
  • Similarly
  • Similarly, protein phosphotyrosine phosphatase obliterated the ability of EGF, but not isoproterenol, to stimulate adenylyl cyclase. (amrita.edu)
  • molecular biology
  • With state-of-the art molecular biology and protein biochemistry labs, we work with our clients to rapidly evaluate in parallel to identify the optimal expression system for candidate proteins. (abgent.com)