Receptor platelet activation. Medical search. Frequent questions

Platelets play a primary role in this process, interacting with subendothelium-bound von Willebrand factor (vWf) via the membrane glycoprotein (GP) Ib complex. (medscape.com)
The process of thrombus formation is considered to be initiated by von Willebrand factor (VWF) binding to exposed collagen or laminin in the damaged vessel wall, followed by shear-dependent platelet binding to VWF through the glycoprotein (GP)Ib-V-IX complex ( 1 , 3 , 4 ). (frontiersin.org)
The flow-dependent adhesion of platelets to those and other extracellular matrix components is stabilized by a panel of integrins, including integrin α 2 β 1 , α IIb β 3 , and α 6 β 1 (adhesive platelet population), while the initial activation of platelets is achieved by signaling via the collagen/laminin receptor, glycoprotein VI (GPVI). (frontiersin.org)
Platelet Glycoprotein GPIIb-IIIa Complex" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
Platelet membrane glycoprotein complex important for platelet adhesion and aggregation. (harvard.edu)
This graph shows the total number of publications written about "Platelet Glycoprotein GPIIb-IIIa Complex" by people in Harvard Catalyst Profiles by year, and whether "Platelet Glycoprotein GPIIb-IIIa Complex" was a major or minor topic of these publication. (harvard.edu)
Below are the most recent publications written about "Platelet Glycoprotein GPIIb-IIIa Complex" by people in Profiles. (harvard.edu)
ii) Determination of the platelet receptor glycoprotein Ib extracellular domain and von Willebrand factor A1 domain structures (iii) determination of the coagulation factor xi structure. (nottingham.ac.uk)
Structural basis of the leukocyte integrin Mac-1 I-domain interactions with the platelet glycoprotein Ib. (nottingham.ac.uk)
Steve's undertakes a multi- and inter-disciplinary to investigate the molecular mechanisms underlying platelet activation by tyrosine kinase linked glycoprotein receptors. (birmingham.ac.uk)
CD44 is an 80-95 kDa type I transmembrane glycoprotein, also known as Phagocytic glycoprotein-1 (Pgp-1), or Extracellular matrix receptor type III (ECMR-III). (bdbiosciences.com)
Glycoprotein IIb/IIIa receptor antagonists block a receptor on the platelet for fibrinogen and von Willebrand factor. (wikidoc.org)
In conclusion, EMP-PDI promotes early platelet activation through glycoprotein (GP) IIb/IIIa receptors present on platelet surface in the diabetic state. (aging-us.com)
However, the ultimate common pathway of platelet aggregation is the activation of glycoprotein (GP) IIb/IIIa receptors present on platelet surface [ 7 - 9 ]. (aging-us.com)
TF is a transmembrane glycoprotein receptor found in extravascular tissues, including organ capsules and the adventitia of blood vessel walls. (merckvetmanual.com)
Human atheromatous plaques stimulate thrombus formation by activating platelet glycoprotein VI. (thieme-connect.com)
VWF binds to receptors on the platelet surface membrane (glycoprotein Ib/IX). (msdmanuals.com)
Platelet receptors for ADP include the P2Y12 receptor, which sends signals to suppress adenylate cyclase, decreases cyclic adenosine monophosphate (cAMP) levels, and promotes activation of the glycoprotein IIb/IIIa receptor (assembled on the activated platelet surface membrane from glycoproteins IIb and IIIa). (msdmanuals.com)
Fibrinogen binds to the glycoprotein IIb/IIIa complexes of adjacent platelets, connecting them into aggregates. (msdmanuals.com)

Platelets are small, a nucleate blood cells, the major role of which is in hemostasis and thrombosis owing to their capacity to adhere to damaged blood vessels and to accumulate at sites of injury ( 1 ). (spandidos-publications.com)
It has been demonstrated clinically that the use of antiplatelet agents to prevent and/or reverse platelet aggregation is a successful strategy for the prevention of thrombosis ( 7 , 8 ). (spandidos-publications.com)
Under physiological circumstances, the resistance of the endothelial cell lining to interactions with platelets and coagulation factors prevents thrombosis. (medscape.com)
According to a common concept of thrombosis and hemostasis, damage or injury of a vessel wall and ensuing exposure of extracellular matrix components to the blood stream triggers platelets from the circulation to become adherent and to assemble into a thrombus, thus limiting the extravasation of blood ( 1 , 2 ). (frontiersin.org)
Dematin Regulates Calcium Mobilization, Thrombosis, and Early Akt Activation in Platelets. (harvard.edu)
If VITT is suspected , perform immediate CBC with platelet count and imaging for thrombosis based on symptoms. (hematology.org)
Alter the platelet activation at the site of vascular damage crucial to the development of arterial thrombosis. (wikidoc.org)
GP IIb/IIIa receptor activation plays an important role in thrombosis. (aging-us.com)
In thrombosis, platelet activation serves as the initiation factor. (aging-us.com)
However, following conformational changes, these receptors are transformed into a high-affinity state wherein they can bind to fibrinogen and promote thrombosis. (aging-us.com)
Lysyl oxidase is associated with increased thrombosis and platelet reactivity. (umassmed.edu)
Platelet PlA2 Polymorphism and the risk for thrombosis in heparin-induced thrombocytopenia. (cdc.gov)

However, while prednisolone (1-10 μM) reduced adenosine diphosphate (ADP, 4 μM) and thromboxane A 2 receptor agonist U46619 induced platelet aggregation (up to 75%), dexamethasone had no effect. (ashpublications.org)
The protease-activated thrombin receptor-1 (PAR-1) can be activated by both the tethered ligand exposed by thrombin cleavage and a synthetic peptide having the tethered ligand sequence (thrombin receptor agonist peptide or TRAP). (nih.gov)
We conducted a mutational analysis of extracellular residues of the receptor potentially involved in interaction with both the tethered ligand and the soluble peptide agonist. (nih.gov)
Agonist-stimulated calcium efflux in X. laevis oocytes or inositol phosphate accumulation in COS-7 cells was used to assess receptor activation. (nih.gov)
Second-generation orally administered thrombopoietin receptor agonist (TPO-RA). (medscape.com)
Oral thrombopoietin (TPO) receptor agonist. (medscape.com)
Here, we observed that HDL isolated from healthy individuals inhibited the production of inflammatory cytokines by peripheral monocytes stimulated with a Toll-like receptor 2 agonist. (lww.com)
Therefore, an inverse agonist of the S1P1 receptor is predicted to reduce signal transduction from PDGFβ receptor tyrosine kinase by blocking the constitutive activity of the G-protein coupled receptor. (strath.ac.uk)
SB649146 is a novel inverse agonist of the S1P1 receptor. (strath.ac.uk)
First, SB649146 displaced the S1P1 receptor agonist dihydrosphingosine 1-phosphate from membranes expressing the recombinant S1P1 receptor. (strath.ac.uk)
These findings are the first to report that a GPCR inverse-agonist reduces growth factor-induced receptor tyrosine kinase signaling, fundamentally broadening their mechanism of action. (strath.ac.uk)

Adherent, activated platelets recruit additional platelets to the growing thrombus ( 5 , 6 ). (spandidos-publications.com)
Once activated, platelets have two major mechanisms to recruit additional platelets to the growing hemostatic plug. (medscape.com)
ADP, thromboxane A2, and other mediators induce activation and aggregation of additional platelets on the injured endothelium. (msdmanuals.com)

Human peripheral blood platelets were stained with CD61 (clone VI-PL2) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram). (biolegend.com)
PRP samples and peripheral blood platelets were then manually counted using a Neubauer chamber. (bvsalud.org)

On resting platelets, GP IIb/IIIa is unable to bind fibrinogen or vWf. (medscape.com)
The alpha granules contain hemostatic proteins such as fibrinogen, vWf, and growth factors (eg, platelet-derived growth factor and transforming growth factors). (medscape.com)
This provokes the release of thromboxane A 2 and the secretion of granular contents including ADP and, via these autocrine agents, the subsequent recruitment of additional flowing platelets, which assemble into a growing thrombus via α IIb β 3 -fibrinogen interactions (aggregating platelet population) ( 1 ). (frontiersin.org)
CD41/CD61 is expressed on platelets and megakaryocytes, and plays a role in platelet activation and aggregation through interaction with fibrinogen, fibronectin, vWF, and other RGD-containing adhesion molecules. (biolegend.com)
Under physiological conditions, the GP IIb/IIIa receptors are present in a low-affinity state and cannot bind to fibrinogen. (aging-us.com)
We investigated, whether GPVI-Fc added in vitro on top of acetylsalicylic acid (ASA), the P2Y 12 antagonist ticagrelor, and the fibrinogen receptor antagonist abciximab alone or in combination would increase inhibition of platelet activation by atherosclerotic plaque. (thieme-connect.com)
GPVI-Fc added on top of abciximab, a clinically used anti-fibrinogen receptor antibody which blocks platelet aggregation, strongly inhibited total (81%) and stable (89%) platelet adhesion. (thieme-connect.com)
Thrombin converts fibrinogen into fibrin monomers, and the fibrin monomers polymerize into fibrin polymers that bind aggregated platelets into platelet-fibrin hemostatic plugs. (msdmanuals.com)
Coagulation factors interact on platelet and endothelial cell surfaces to produce thrombin , which converts fibrinogen to fibrin. (msdmanuals.com)
Platelet FcgammaRIIA His131Arg polymorphism and platelet function: antibodies to platelet-bound fibrinogen induce platelet activation. (cdc.gov)

Data from a radiolabeled ligand‑binding assay indicated that LGP exhibited apparent competing effects on thromboxane receptor (TP) and P2Y12 receptors. (spandidos-publications.com)
These inhibitory effects may be associated with its dual‑receptor inhibition on P2Y12 and TP receptors. (spandidos-publications.com)
Clopidogrel exerts its antiplatelet effect by inhibiting the binding of adenosine diphosphate (ADP) to its receptor (P2Y12) and consequent ADP-mediated platelet activation [ 3 ]. (hindawi.com)
In combination with aspirin, inhibition of the platelet P2Y12 ADP receptor with clopidogrel has become a cornerstone in the antiplatelet in acute coronary syndromes. (avhandlingar.se)
We also investigate the effects of a novel P2Y12 receptor antagonist, prasugrel, in comparison to clopidogrel. (avhandlingar.se)
In a subsequent study we could correlate decreased response to clopidogrel to increased protein expression of the P2Y12 receptor in patients with coronary artery disease. (avhandlingar.se)

Using a platelet‑aggregation assay, it was revealed that LGP significantly inhibited platelet aggregation induced by ADP, U46619 and arachidonic acid. (spandidos-publications.com)

Upon vascular injury, platelets are exposed to the subendothelium, and several agonists, including adenosine diphosphate (ADP) and thrombin, are generated at the injury site, which can stimulate platelet adhesion, activation and aggregation. (spandidos-publications.com)
During activation, platelets release mediators of aggregation, including adenosine diphosphate (ADP) from storage granules. (msdmanuals.com)

Ramatroban is an orally bioavailable, potent, dual antagonist of the thromboxane A 2 (TPr) and PGD 2 (DPr2) receptors. (researchsquare.com)

They pass through the cell membrane seven times in the form of six loops (three extracellular loops interacting with ligand molecules, three intracellular loops interacting with G proteins, an N-terminal extracellular region and a C-terminal intracellular region) of amino acid residues, which is why they are sometimes referred to as seven-transmembrane receptors. (wikipedia.org)
Ligands can bind either to the extracellular N-terminus and loops (e.g. glutamate receptors) or to the binding site within transmembrane helices (rhodopsin-like family). (wikipedia.org)
Seven years later, the crystallization of β2-adrenergic receptor (β2AR) with a diffusible ligand brought surprising results because it revealed quite a different shape of the receptor extracellular side than that of rhodopsin. (wikipedia.org)
Several point mutations and deletion of extracellular domains (DeltaEC3, DeltaNH3) drastically altered the ability of mutant receptors to respond to TRAP, but not to thrombin, and did not affect the affinity for the radiolabeled TRAP by these mutant receptors. (nih.gov)
Only mutations that disrupted the putative disulfide bond or substitution of multiple acidic residues in the second extracellular loop by alanine had a significant effect on both ligand binding and thrombin activation. (nih.gov)
CD51/CD61 is expressed on platelets, osteoclasts, fibroblasts, macrophages, and some tumor cells involved in tumor metastasis, and in adenovirus infection through binding to RGD motif in extracellular matrix proteins. (biolegend.com)

The crucial role of platelets in maintaining primary hemostais has been evident for a long time and there is now also mounting evidence for their involvement in inflammation. (avhandlingar.se)
Heparin-induced thrombocytopenia: the role of platelets genetic polymorphisms. (cdc.gov)

The hemostatic system consists of platelets, coagulation factors, and the endothelial cells lining the blood vessels. (medscape.com)
Initial platelet adhesion is to long strings of von Willebrand factor (VWF) that have been previously secreted by, and anchored to, stimulated endothelial cells. (msdmanuals.com)
They are found primarily on PLATELETS and on ENDOTHELIAL CELLS. (bvsalud.org)

Platelet activation allows binding of these proteins, which bridges adjacent platelets. (medscape.com)
G protein-coupled receptors (GPCRs), also known as seven-(pass)-transmembrane domain receptors, 7TM receptors, heptahelical receptors, serpentine receptors, and G protein-linked receptors (GPLR), form a large group of evolutionarily related proteins that are cell surface receptors that detect molecules outside the cell and activate cellular responses. (wikipedia.org)
The ligands that bind and activate these receptors include light-sensitive compounds, odors, pheromones, hormones, and neurotransmitters, and vary in size from small molecules to peptides to large proteins. (wikipedia.org)
We describe how these are activated, which are their target proteins, and how their proteolytic activity modulates platelet functions. (frontiersin.org)
These proteins are vital to normal platelet adhesion and hemostatic function. (nottingham.ac.uk)
Hematopoietic cells contain more than a dozen different GPI-anchored proteins including adhesion molecules, enzymes, and receptors. (aacc.org)
Prothrombinase assembly on platelets is not strictly a function of phospholipid composition, but is likely coordinated by one or more specific binding proteins. (medscape.com)
Investigators have demonstrated other substrates for factor XIIIa, including proteins such as osteopontin, factor V, thrombospondin, vinculin, and endothelial cell receptors α v β 3 integrin and VEGFR-2. (medscape.com)

The platelets arise from the fragmentation of the cytoplasm of megakaryocytes in the bone marrow and circulate in blood as disc-shaped anucleate particles for 7-10 days. (medscape.com)
Megakaryocytes (MKs) are the cellular source of platelets. (elifesciences.org)
CD42b is expressed on platelets and megakaryocytes. (biolegend.com)
Stimulates proliferation and differentiation of megakaryocytes from bone marrow progenitor cells, resulting in an increased production of platelets. (medscape.com)

Steve's research interests focus on mechanisms of platelet activation by tyrosine kinase-linked receptors (notably CLEC-2, FcgammaRIIA, GPVI and PEAR1) and in developing novel antiplatelet agents that target these receptors in thrombi-inflammatory disease. (birmingham.ac.uk)

In contrast, thrombin receptor-activating peptides derived from the human PAR 1 receptor sequence (e.g. (aspetjournals.org)
Characterization of a functional thrombin receptor. (jci.org)
A cryptic peptide ligand contained within the N-terminal of the thrombin receptor. (bvsalud.org)

The uncontrolled progression of these processes through a series of self-sustaining amplification loops can initiate unrestrained platelet activation and aggregation, and eventually lead to thromboembolic events ( 7 , 8 ). (spandidos-publications.com)

To investigate whether the administration of renin-angiotensin system (RAS) inhibitors and statins could alleviate atrial fibrosis via platelet-derived growth factor (PDGF)/Rac1 /nuclear factor-kappa B (NF-κB) axis. (medsci.org)
In addition, other studies demonstrated that AngII activated platelet-derived growth factor-A (PDGF-A), a novel contributor to cardiac remodelling, to result in atrial fibrosis in pressure-overloaded mouse model [ 13 ] . (medsci.org)
PDGFR-α is a high-affinity receptor for each of the three PDGF forms. (peprotech.com)
We have previously identified a novel complex between the platelet-derived growth factor (PDGF)β receptor and the sphingosine 1-phosphate receptor-1 (S1P1). (strath.ac.uk)
The complex permits the utilization of active G-protein subunits (made available by constitutively active S1P1 receptor) by the PDGFβ receptor kinase to transmit signals to p42/p44 MAPK in response to PDGF. (strath.ac.uk)
We now report that inverse agonism of the S1P1 receptor with SB649146 reduced the endocytosis of the PDGFβ receptor-S1P1 receptor complex and the stimulation of p42/p44 MAPK and cell migration in response to PDGF. (strath.ac.uk)

5 Since changes in de novo protein synthesis are the basis for glucocorticoid effectiveness, the lack of a modulatory effect on platelet functions is not surprising given that these cells have been regarded as incapable of synthetic activity. (ashpublications.org)
We describe how these are activated, their targets, their effect on platelet functions, and the consequences for platelet population formation. (frontiersin.org)

In the other receptors crystallized shortly afterwards the binding side was even more easily accessible to the ligand. (wikipedia.org)
Ligand-binding studies revealed the presence of a high- and low-affinity binding site for [ 3 H]-dexamethasone in platelets. (ashpublications.org)
RU486), suggesting the functional importance of the ligand-receptor complex. (ashpublications.org)
This process relies on the destruction of disulfide bonds present on these receptors, such as Cys5-Cys435, Cys663-Cys687, and a third Cys-rich repetitive zone, to be transformed into the ligand-binding, high-affinity state and evoke conformational changes in GP IIb/IIIa receptors [ 10 ]. (aging-us.com)
Activation of thrombin receptors occurs through the proteolytic action of THROMBIN, which cleaves the N-terminal peptide from the receptor to reveal a new N-terminal peptide that is a cryptic ligand for the receptor. (bvsalud.org)

It was also found that LGP exhibited marked inhibitory effects on the activation of αIIbβ3 integrin, the secretion of serotonin from granules, and the synthesis of thromboxane A2. (spandidos-publications.com)
Thromboxane A 2 also increases vascular permeability, contracts bronchial smooth muscle, triggers and amplifies platelet activation, and promotes a prothrombotic state. (researchsquare.com)
Aspirin irreversibly inhibits the enzyme COX, resulting in reduced platelet production of TXA 2 (thromboxane - powerful vasoconstrictor which lowers cyclic AMP and initiates the platelet release reaction). (wikidoc.org)

Therefore, understanding the molecular mechanisms of platelet activation and identifying novel techniques for platelet inhibition remain critically important. (spandidos-publications.com)
New structures complemented with biochemical investigations uncovered mechanisms of action of molecular switches which modulate the structure of the receptor leading to activation states for agonists or to complete or partial inactivation states for inverse agonists. (wikipedia.org)
The activities of adhesion and signaling receptors in platelets are controlled by several mechanisms. (frontiersin.org)
Platelets and serum were treated with different inhibitors to determine mechanisms involved in platelet aggregation and activation. (lu.se)
To the best of our knowledge, this is the first study that reports on platelet interactions of SBSEC-isolates other than Streptococcus gallolyticus subspecies gallolyticus as well as the first study to report of mechanisms of platelet interaction of SBSEC-isolates. (lu.se)
Many recent studies in both animal models and humans have revealed that renin-angiotensin system (RAS) activation is one of the most vital mechanisms involved in atrial fibrosis [ 1 , 4 ] . (medsci.org)
Steve uses advanced microscopy (FCS and single molecule), molecular modelling (ODE and agent-based), crystallography, cell line studies and protein phosphorylation to study the mechanisms of receptor activation. (birmingham.ac.uk)
In the last paper we investigate the mechanisms behind platelet-endothelial cell interactions in a mouse model of arterial inflammation. (avhandlingar.se)
Various mechanisms, including endothelial cell nitric oxide and prostacyclin, promote blood fluidity by preventing platelet aggregation and dilating intact blood vessels. (msdmanuals.com)

[ 8 ] Although any membrane surface that expresses anionic phospholipid can support prothrombinase complex assembly, the activated platelet surface is especially well suited for this purpose. (medscape.com)

The platelet GP IIb/IIIa complex mediates platelet-to-platelet interactions (platelet aggregation). (medscape.com)
SBSEC mediates platelet aggregation in an IgG and IgG-Fc-receptor dependent manner. (lu.se)

G protein-coupled receptors are found only in eukaryotes, including yeast, and choanoflagellates. (wikipedia.org)
G protein-coupled receptors are involved in many diseases. (wikipedia.org)
There are two principal signal transduction pathways involving the G protein-coupled receptors: the cAMP signal pathway and the phosphatidylinositol signal pathway. (wikipedia.org)
With the determination of the first structure of the complex between a G-protein coupled receptor (GPCR) and a G-protein trimer (Gαβγ) in 2011 a new chapter of GPCR research was opened for structural investigations of global switches with more than one protein being investigated. (wikipedia.org)
These observations presented a spatial and temporal resolution of the sequence of events underlying receptor-evoked involvement of tubulin in G-protein-mediated signaling. (jneurosci.org)
It is suggested that G-protein-coupled receptors might modulate cytoskeletal dynamics, intracellular traffic, and cellular architecture. (jneurosci.org)
When blood vessels are damaged by a cut or by other types of vascular injury, VWF in blood plasma binds to the site of damage and unravels to form long protein strings that specifically capture specialised blood cells (platelets) to the site of injury. (nottingham.ac.uk)
Long-term activation of protein kinase c causes chronic Na/H antiporter stimulation in cultured proximal tubule cells. (jci.org)
Short-term activation of protein kinase C by 5 min exposure to PMA caused an acute increase in Na/H antiporter activity that was not prevented by cycloheximide or actinomycin D and did not persist 24 h later. (jci.org)
Long-term activation of protein kinase C by 2 h exposure to PMA caused a dose-dependent increase in Na/H antiporter activity 24 h later. (jci.org)
This latter effect was due to protein kinase C activation in that it was inhibited by sphingosine and was not seen with 4 alpha-PMA, an inactive analogue. (jci.org)
In conclusion, short-term activation of protein kinase C leads to a transient increase in Na/H antiporter activity that is independent of transcription and translation, whereas long-term activation of protein kinase C causes a persistent increase in antiporter activity that is dependent on transcription and translation and is associated with increased mRNANa/H abundance. (jci.org)
An Fc-peptide fusion protein (peptibody) that increases platelet production through binding and activation of the thrombopoietin (TPO) receptor, a mechanism similar to endogenous TPO. (medscape.com)
The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. (avivasysbio.com)
We used real-time RT-PCR and quantitative western blots to study mRNA and protein expression levels in human platelets. (avhandlingar.se)
Qin R , Zhu H , Wang F , Song M , Lin P , Xing Y , Zhang W , Zhong M , Wang Z , . Platelet activation in diabetic mice models: the role of vascular endothelial cell-derived protein disulfide isomerase-mediated GP IIb/IIIa receptor activation. (aging-us.com)
The purpose of this study was to investigate the release of Endothelial microparticle (EMP)-associated protein disulfide isomerase (PDI) after endothelial cell injury induced in diabetes and the changes in platelet activation. (aging-us.com)
GPVI-Fc, a recombinant dimeric fusion protein binding to plaque collagen and concealing binding sites for platelet GPVI, acts as a lesion-focused antiplatelet drug, and does not increase bleeding in vivo. (thieme-connect.com)
In its active form, clopidogrel prevents (inhibits) the function of a receptor protein known as P2RY12 that is found on the surface of platelets. (medlineplus.gov)
the P2RY12 receptor protein helps platelets cluster together to form a clot to seal off damaged blood vessels and prevent blood loss. (medlineplus.gov)
BACKGROUND: The protein kinase B (AKT)/mammalian target of rapamycin (mTOR) signaling pathway regulates early follicular activation and follicular pool maintenance in female germline cells. (bvsalud.org)
Polymorphisms of protein tyrosine phosphatase CD148 influence Fc?RIIA-dependent platelet activation and the risk of heparin-induced thrombocytopenia. (cdc.gov)
Platelet-dependent neutrophil function is dysregulated by M protein from Streptococcus pyogenes. (lu.se)

The mechanism of early activation of GP IIb/IIIa receptors in diabetic conditions remains unknown. (aging-us.com)
The EMP-PDI content and GP IIb/IIIa expression of mice platelets were determined. (aging-us.com)
Compared with the normal diet group, the diabetic group had significantly increased plasma EMP-PDI content and accelerated platelet activation by increased GP IIb/IIIa expression. (aging-us.com)
In contrast, the strong inhibition of platelet adhesion by GPVI-Fc in combination with GPIIb/IIIa inhibitors could be harmful. (thieme-connect.com)
The antigenic target in most patients appears to be the platelet GP IIb/IIIa complex. (medscape.com)

Bacterial activation of platelets through this pathway is common for many bacteria causing IE and could be a potential therapeutic target for the prevention and treatment of this infection. (lu.se)
The Fc-IgG-receptor pathway was shown essential for platelet activation and aggregation. (lu.se)
This was studied in SK-N-SH neuroblastoma cells, which possess a muscarinic receptor-regulated PLCβ 1 -signaling pathway. (jneurosci.org)
The alternate complement pathway is under continuous state of activation. (aacc.org)
It is also due to disrupted fibrinolysis and tissue factor inhibitor pathway because of the lack of GPI-anchored receptors and cofactors. (aacc.org)

We could conclude that prasugrel inhibits several markers of platelet activation and the formation of platelet-leukocyte aggregates more efficiently than clopidogrel. (avhandlingar.se)
Potently inhibits ADP-induced platelet aggregation and P-selectin expression in vitro (pIC 50 values are 8.26 and 8.56, respectively). (tocris.com)

Peripheral versus central venous blood sampling does not influence the assessment of platelet activation in cirrhosis. (harvard.edu)
The primary endpoint was assessment of platelet aggregation after stimulation with adenosine diphosphate 20 μM at 12-month follow-up. (revespcardiol.org)

Neither affinity-purified Ab1 nor Ab2 binds to platelets. (jci.org)
However, the combination of Ab1 and Ab2 (preincubated for 2 h at 22 degrees C) binds to platelets in a saturation-dependent manner at an optimum ratio range of 10-20:1. (jci.org)
FIXa on platelets and TF-bearing cells binds with FVIIIa, assembling the FIXa-FVIIIa complex (intrinsic tenase). (merckvetmanual.com)

The abilities of the PAR 1 -targeted agents to desensitize or block PAR 1 in the HEK cell assay were compared with their activities in a human platelet aggregation assay. (aspetjournals.org)

In addition, when platelets are activated, negatively charged phospholipids move from the inner to the outer leaflet of the membrane bilayer. (medscape.com)
We provide a comprehensive overview of the proteases acting on the platelet membrane. (frontiersin.org)
Upon prolonged high intracellular rises in Ca 2+ , platelets develop a procoagulant phenotype that is characterized by the surface membrane exposure of phosphatidylserine and by the inactivation of integrin α IIb β 3 (procoagulant platelet population) ( 5 ). (frontiersin.org)
Phosphatidylserine-exposing platelets, usually located around a thrombus, provide a negatively charged membrane surface, which supports coagulation factor binding and the formation of tenase and prothrombinase complexes ( 6 , 7 ). (frontiersin.org)
Here, we review current knowledge how proteases act on platelet receptors and the platelet membrane surface. (frontiersin.org)
Intracytoplasmic neutrophils develop membrane contiguity with the demarcation membrane system, thereby transferring membrane to the megakaryocyte and to daughter platelets. (elifesciences.org)
This phenomenon occurs in otherwise unmanipulated murine marrow in vivo, resulting in circulating platelets that bear membrane from non-megakaryocytic hematopoietic donors. (elifesciences.org)
These findings identify emperipolesis as a new cell-in-cell interaction that enables neutrophils and potentially other cells passing through the megakaryocyte cytoplasm to modulate the production and membrane content of platelets. (elifesciences.org)

We developed a calcium signaling-based assay, using cultured human embryonic kidney cells (HEK), that evaluates simultaneously, the activation/desensitization or blockade of the proteinase-activated receptors, PAR 1 and PAR 2 . (aspetjournals.org)
A family of proteinase-activated receptors that are specific for THROMBIN. (bvsalud.org)

This initial interaction (platelet adhesion) sets the stage for other adhesive reactions that allow the platelets to interact with other agonists in the vicinity of vessel injury, such as adenosine 5'-diphosphate (ADP), subendothelial collagen, and thrombin. (medscape.com)

Atherosclerosis starts with dysfunctional changes in the endothelium induced by disturbed shear stress which can lead to endothelial and platelet activation, adhesion of monocytes on the activated endothelium, and differentiation into proinflammatory macrophages, which increase the uptake of oxidized LDL (oxLDL) and turn into foam cells, exacerbating the inflammatory signalling. (hindawi.com)
Therefore, with the aim to summarize the current knowledge on the initiation of the atherosclerotic process, in this paper, we review the early markers of atherosclerosis and we address the main therapeutic targets for preventing atheroma formation at its very initial stages focusing on inflammation, oxidative stress, endothelial dysfunction, and the interaction between platelets and endothelium. (hindawi.com)
TM is a cell surface receptor for thrombin, mainly expressed on undamaged endothelium. (merckvetmanual.com)

The body's reaction to vessel wall injury is rapid adhesion of platelets to the subendothelium. (medscape.com)

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections, Westerm blotting, and inhibition of platelet aggregation 2 . (biolegend.com)

This initial interaction (platelet adhesion) sets the stage for other adhesive reactions that allow the platelets to interact with each other to form an aggregate (see image below). (medscape.com)

However, diabetic patients have significantly higher platelet aggregation and activation than non-diabetic patients, including those undergoing dual antiplatelet therapy [ 4 - 6 ]. (aging-us.com)
The identification of the key molecules that accurately regulate the activation of these receptors may provide a novel approach for antiplatelet therapy. (aging-us.com)
Clopidogrel (also known as Plavix) is an antiplatelet drug, which means that it prevents blood cells called platelets from sticking together (aggregating) and forming blood clots . (medlineplus.gov)

Platelet activation and subsequent accumulation at sites of vascular injury are central to thrombus formation, which is considered to be a trigger of several cardiovascular diseases. (spandidos-publications.com)
We also describe how the proteolytic activities are determined by different platelet populations in a thrombus and conversely how proteolysis contributes to the formation of such populations. (frontiersin.org)
Within a thrombus, however, distinct types of activated platelets can be recognized, exhibiting different functions, although partial overlap between the populations exists ( 3 ). (frontiersin.org)
Thrombin, which is generated at these phosphatidylserine sites, triggers the formation of fibrin fibers, which consolidate the platelet thrombus into a stable clot sealing the breach in a vessel wall ( 2 ). (frontiersin.org)

Platelet Activation and Reactivity in a Large Cohort of Patients with Gaucher Disease. (harvard.edu)
Intensive glucose control with insulin in patients with an acute coronary syndrome reduces platelet reactivity during hospitalization, compared to conventional control. (revespcardiol.org)
However, the effect of strict, long-term glucose control on platelet reactivity in these patients remains uncertain. (revespcardiol.org)
180 mg/dL), on platelet reactivity after hospital discharge in patients with an acute coronary syndrome and hyperglycemia. (revespcardiol.org)

Without active clopidogrel to interfere, the P2RY12 receptor continues to promote platelet aggregation and blood clot formation, which can lead to heart attacks, strokes, and thromboses in individuals with a history of these conditions. (medlineplus.gov)
platelets and fibrin combine to form a clot. (msdmanuals.com)
As you know, I think the most famous part about platelet function is the ability of your blood to clot. (cdc.gov)

Platelet activation pathways. (medscape.com)
The Streptococcus bovis/Streptococcus equinus complex (SBSEC) contains known IE-pathogens, but there is limited research on the different subspecies ability to interact with platelets and what signaling pathways are involved. (lu.se)

CD44 is expressed on leucocytes, erythrocytes, epithelial cells and weakly on platelets. (bdbiosciences.com)

Thus, the release reaction and prostaglandin synthesis act to consolidate the initial hemostatic plug by promoting the participation of other platelets in the growing hemostatic plug. (medscape.com)
The initial hemostatic plug, composed primarily of platelets, is stabilized further by a fibrin mesh generated in secondary hemostasis. (medscape.com)
Shear-Mediated Platelet Microparticles Demonstrate Phenotypic Heterogeneity as to Morphology, Receptor Distribution, and Hemostatic Function. (harvard.edu)

SLAM is also expressed by hematopoietic stem cells, dendritic cells and platelets and is a T cell measles virus receptor. (rndsystems.com)

It is due to platelet activation through nitric oxide depletion and complement mediated activation through loss of CD59. (aacc.org)
Nanoparticle inhalation modulates arteriolar sympathetic constriction: role of nitric oxide, prostanoids, and alpha-adrenergic receptors. (cdc.gov)

Emperipolesis is amplified in models of murine inflammation associated with platelet overproduction, contributing to platelet production in vitro and in vivo. (elifesciences.org)

It is a member of the integrin family, associating with platelet gpIIb (CD41) to form CD41/CD61 complex and with integrin aV (CD51) to form aV/ß3 (CD51/CD61) integrin. (biolegend.com)

The apparently anomalous binding data were clarified by the fact that washed platelets (1) contained mineralocorticoid receptor and that (2) it was associated with GR. Taken together, our data suggest that platelet GR forms a heterodimeric complex with the mineralocorticoid receptor that is susceptible to differential activation by specific receptor ligands. (ashpublications.org)
We suggest that the anti-HIV-1gp120 antiidiotype-like complex contributes to the markedly elevated platelet Ig and C3 level of HIV-1-ITP patients and propose that this may contribute to their thrombocytopenia. (jci.org)
CD42b/c heterodimer forms a complex with CD42a and d and acts as the receptor for von Willibrand factor and thrombin. (biolegend.com)
Upon activation by thrombin, FVIIIa dissociates from the FVIII-vWF complex to interact with FIXa. (merckvetmanual.com)

It is constitutively expressed on fibroblasts and, on cellular activation, on vascular smooth muscle cells, monocytes, and neutrophils. (merckvetmanual.com)
A 2 dimers are present in circulating platelets and monocytes. (medscape.com)

A new role for the A2b adenosine receptor in regulating platelet function. (umassmed.edu)

Under static conditions, GPVI-Fc inhibited plaque-induced platelet aggregation by 53%, and increased platelet inhibition by ASA (51%) and ticagrelor (64%) to 66% and 80%, respectively. (thieme-connect.com)
Under arterial flow, GPVI-Fc inhibited plaque-induced platelet aggregation by 57%, and significantly increased platelet inhibition by ASA (28%) and ticagrelor (47%) to about 81% each. (thieme-connect.com)
GPVI-Fc alone or in combination with ASA or ticagrelor did not increase closure time measured by the platelet function analyzer (PFA)-200. (thieme-connect.com)

These interactions further activate platelets. (medscape.com)
For IE-pathogens, the capacity to activate and aggregate platelets is believed to be an important virulence mechanism. (lu.se)
IMPORTANCE The capacity of bacteria to activate and aggregate platelets is believed to contribute to the pathogenesis of IE. (lu.se)
It adds SBSEC to a group of bacteria that activate and aggregate platelets via the platelet Fc-receptor. (lu.se)
Small synthetic peptides that contain the unmasked N-terminal peptide sequence can also activate the receptor in the absence of proteolytic activity. (bvsalud.org)

Under these conditions, platelets adhere to the damaged intima and form aggregates. (msdmanuals.com)

Cardiovascular (CV) risk factors such as hypercholesterolemia, hyperglycaemia, obesity, hypertension, smoking, and aging promote vascular inflammation and endothelial activation [ 7 - 9 ]. (hindawi.com)

Blockade of the platelet Fc-receptor or enzymatic cleavage of IgG abolished platelet activation and aggregation. (lu.se)

We previously demonstrated that human platelets activated with SFLLRN release PAR-1 activation peptide, PAR-1-(1-41), even in the presence of hirudin. (mcmaster.ca)

While the interactions between bacteria and platelets have been described in detail for many Gram-positive pathogens, little research has been carried out with SBSEC in this respect. (lu.se)
Twenty-six isolates of the four most common species and subspecies of SBSEC identified in bacteremia were collected, and interactions with platelets were investigated in platelet rich plasma (PRP) from three donors. (lu.se)
To study platelet interactions in vivo in mice we used fluorescent intravital microscopy. (avhandlingar.se)
Furthermore, in a moue model of arterial inflammation we found that P-selectin is responsible for a significant part of the platelet-leukocyte interactions. (avhandlingar.se)

Few studies have addressed the effects of classical anti-inflammatory glucocorticoids on platelet function. (ashpublications.org)
Despite their extensive use in cardiovascular and immune pathologies, glucocorticoids have been reported to be largely inactive on platelet function. (ashpublications.org)
4 Similarly, conventional clinical doses of prednisone do not impair platelet function or enhance primary hemostasis in healthy subjects, as measured by the bleeding time. (ashpublications.org)
An important way of regulation is provided by proteolytic cleavage of several of these receptors, leading to either a gain or a loss of platelet function. (frontiersin.org)
Although these platelet activation processes are relatively well-studied as a function of the platelet environment, only since recently it is becoming clear that a multitude of proteases present in plasma or produced by platelets themselves are important for the distinct properties of platelet populations, often by cleaving specific receptors. (frontiersin.org)
Trpc6 gain-of-function disease mutation enhances phosphatidylserine exposure in murine platelets. (harvard.edu)
There were no differences between groups in baseline characteristics or platelet function. (revespcardiol.org)
To study platelet function we mainly used flow cytometry based techniques. (avhandlingar.se)
Platelets are also very important in maintaining this barrier function. (cdc.gov)
Well, platelets have that function as well as many other functions to maintain that blood vessel system. (cdc.gov)

Interacts with transmembrane domain of human TPO receptor and induces megakaryocyte proliferation and differentiation from bone marrow progenitor cells. (medscape.com)
regulate megakaryocyte maturation, platelet activation, and transcript profile during inflammation in mice and humans. (umassmed.edu)
Platelet and Megakaryocyte Roles in Innate and Adaptive Immunity. (umassmed.edu)

CD150) was the first identified of a family of type I transmembrane (TM) lymphocyte activating receptors. (rndsystems.com)

The capacity for bacteria-induced platelet aggregation was also shown to be transferable between donors through serum. (lu.se)
Upon cellular activation, the bacteria then adhere to chemokine receptors, particularly the platelet-activating factor receptor. (stjude.org)

Aggregation was studied using light-transmission aggregometry and platelet activation using flow cytometry detecting. (lu.se)
Aggregation was studied using light-transmission aggregometry and platelet activation using flow cytometry detecting surface upregulation of CD62P. (lu.se)

Anatomy14

Organisms7

Diseases18

Chemicals and Drugs130

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes31

Disciplines and Occupations1

Information Science2

Health Care1

Glycoprotein19

Thrombosis12

Agonist11

Additional platelets3

Blood Platelets2

Fibrinogen10

P2Y126

Inhibited platelet aggregation1

Including adenosine diphosphate2

Antagonist1

Extracellular6

Role of platelets2

Endothelial cells3

Proteins8

Megakaryocytes4

Tyrosine kinase1

Thrombin receptor3

Unrestrained platelet activation and aggregation1

PDGF6

Effect on platelet functions2

Ligand5

Thromboxane3

Mechanisms9

Activated platelet surface1

Mediates2

Protein23

IIIa5

Pathway5

Inhibits2

Assessment of platelet2

Binds3

Human platelet1

Membrane8

Proteinase-activated receptors2

Collagen1

Endothelium3

Adhesion of platelets1

Inhibition of platelet1

Interaction1

Antiplatelet3

Thrombus4

Reactivity4

Clot3

Pathways2

Epithelial1

Hemostatic3

Hematopoietic stem1

Nitric oxide2

Vitro1

Integrin1

Complex4

Monocytes2

Adenosine receptor1

GPVI3

Activate5

Aggregates1

Vascular1

Blockade1

Peptide1

Interactions4

Function10

Megakaryocyte3

Transmembrane1

Bacteria2

Flow cytometry2