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  • target genes
  • Then, 5 potential target genes with tumor-promoting function (AMFR, NOTCH1, HNRNPF, TOP1, and LAPTM4B) were selected from the 31 genes and their 3′UTRs containing the complementary binding sites of miR-139-5p were cloned into a luciferase reporter vector to evaluate the influence of miR-139-5p on the expression of a reporter gene using a luciferase assay (Fig. 3A). (nih.gov)
  • Notch1 and its target genes were downregulated in all treated groups. (omicsonline.org)
  • expression
  • Transient transfection of mutagenized NOTCH1 missense constructs also revealed significant reduction in gene expression. (qmul.ac.uk)
  • We found that miR-139-5p was significantly downregulated in 73.8% CRC samples compared with adjacent noncancerous tissues (NCTs), and decreased miR-139-5p was associated with poor prognosis.Furthermore, the protein levels of the two genes were upregulated in CRC samples compared with NCTs, and inversely correlated with the miR-139-5p expression.Increased NOTCH1 protein expression was correlated with poor prognosis of CRC patients. (nih.gov)
  • Increased NOTCH1 protein expression was correlated with poor prognosis of CRC patients. (nih.gov)
  • We reasoned that the potential for OPN to affect stem cell function in the niche was great given its abundance in the proper geographic location, its receptor expression on stem cells, and the evidence for it affecting processes in other cells that might be relevant for stem cell physiology. (rupress.org)
  • The expression of Notch1 in vivo was enhanced in response to injury. (jneurosci.org)
  • vitro
  • In vitro T cell development of Pax5-/- progenitors strictly depends on Notch1 function and progresses through normal developmental stages by expressing T cell markers and rearranging TCRbeta, gamma, and delta loci in the correct temporal sequence. (epfl.ch)
  • Activation
  • We showed that activation of the osteoblast by parathyroid hormone (PTH)-R activation could increase stem cell numbers mediated by Notch1. (rupress.org)
  • findings
  • CONCLUSIONS: These findings highlight a key role for NOTCH1 across a range of developmental anomalies that include cardiac defects and implicate NOTCH1 haploinsufficiency as a likely molecular mechanism for this group of disorders. (qmul.ac.uk)
  • amino
  • A synthetic peptide corresponding to residues surrounding amino acids 1760 of mouse Notch1. (abnova.com)
  • stem cells
  • Stem cells are known to express CD44 and α4 integrin, both of which are receptors capable of interacting with OPN ( 12 , 13 ). (rupress.org)
  • functional
  • Five downregulated genes (AMFR, NOTCH1, HNRNPF, TOP1, and LAPTM4B) were selected from the 31 genes in the initial screening based on the functional analysis of these genes, and their 3′UTRs were assessed using the luciferase reporter assay. (nih.gov)
  • however, silencing by RNA interference demonstrated that all receptors are functional. (ahajournals.org)
  • Mutation
  • A mutation was generated in the site complementary to the miR-139-5p seed region of the 3′UTR of AMFR or NOTCH1, as indicated. (nih.gov)
  • data suggest
  • These data suggest that Notch1 acts upstream of GATA3 and Tcf1 in early T cell development and regulates Vbeta-DJbeta rearrangements by controlling the chromatin accessibility of Vbeta genes at the TCRbeta locus. (epfl.ch)
  • family
  • Congenital heart anomalies were noted in 47% (8/17) of NOTCH1-positive probands and affected family members. (qmul.ac.uk)