Receptor cell biology clone cells. Medical search. Frequent questions

While innate immune cells are important for early tumor immune surveillance, T cells are fundamentally recognized for their crucial role in the antigen-specific recognition and elimination of malignantly transformed cells ( 2 ). (frontiersin.org)
T cell memory is antigen-specific, and can provide durable host-wide protection. (frontiersin.org)
In the early 2000's, large and persistent populations of antigen (Ag)-specific CD8 T cells in peripheral tissues were initially classified as T EM cells in recirculation from the blood ( 8 , 11 ). (frontiersin.org)
The T45-2342 monoclonal antibody recognizes the mouse F4/80 antigen which is also known as EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1). (bdbiosciences.com)
Joel saw his first image of an immune synapse, the specialized interface that T cells form at the contact with an activating antigen presenting cell. (yale.edu)
This serves as a guidepost for rationally designing new combination checkpoint therapies, downstream T cell receptor (TCR) isolation, cancer antigen identification and molecular characterization of naturally occurring tumor-reactive T cells in human cancer. (upenn.edu)
Another approach relies upon the use of chimeric antigen receptors (CARs) that directly target cancer cells or immunosuppressive cellular elements in the tumor microenvironment. (upenn.edu)
Neoadjuvant anti-OX40 (MEDI6469) therapy in patients with head and neck squamous cell carcinoma activates and expands antigen-specific tumor-infiltrating T cells. (bepress.com)
Analyses of CD8+ TIL show increases in tumor-antigen reactive, proliferating CD103+ CD39+ cells in 25% of patients with evaluable tumor tissue (N = 4/16), all of whom remain disease-free. (bepress.com)
We have examined the T cell receptor (TcR) expression of clones specific for epitopes of mycobacterial 65-kDa heat-shock protein (hsp65) in the context of two different HLA molecules, and used this system as a model to assess the selection of T cells responsive to this antigen in vivo. (ox.ac.uk)
Sequencing alpha and beta chains of representative V beta 5.2+ and V beta 5.6+ PB-derived clones revealed TcR which were identical to those utilized by the SF-derived clones, showing that the repertoire for recognition of this antigen is stable over time. (ox.ac.uk)
The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes. (biolegend.com)
GL7 high-expressing B cells show higher antibody production and antigen presenting capacity. (biolegend.com)
Then they pass to the thymus gland (sits above the heart), where the T-lymphocytes acquire their receptors specific to an antigen. (scienceaid.net)
The rapid reproduction of a specific B cell is caused by a macrophage that has an antigen in its membrane coming into contact with the B cell. (scienceaid.net)
Chimeric antigen receptor (CAR) T-cell therapy has offered a novel approach to treating hematologic cancers since 2017, but there are early signs that these cellular immunotherapies could be repurposed for B-cell mediated autoimmune diseases. (medscape.com)
B-cell targeted therapies have been around since the early 2000s with drugs like rituximab , a monoclonal antibody medication that targets CD20, an antigen expressed on the surface of B cells. (medscape.com)
The CAR T cells currently available target another surface antigen, CD19, and are a much more potent therapy. (medscape.com)
In this process, T cells are collected from patients via leukapheresis and then re-engineered to express chimeric antigen receptors. (medscape.com)
Murine monoclonal antibodies are produced by injecting a mouse with an antigen, harvesting its spleen to obtain B cells that are producing antibody specific to that antigen, fusing those cells with immortal mouse myeloma cells, growing these hybridoma cells (eg, in cell culture), and harvesting the antibody. (msdmanuals.com)
In both cases, the process usually begins as above with production of mouse hybridoma cells that make antibody to the desired antigen. (msdmanuals.com)
Chimeric monoclonal antibodies activate antigen-presenting cells (APCs) and T cells more effectively than murine monoclonal antibodies but can still induce production of human anti-chimeric antibodies. (msdmanuals.com)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
Prostate-specific antigen (PSA) is a protein produced by normal prostate cells. (medscape.com)

Recently, a fourth member of the bombesin (Bn) receptor family (fBB 4 -R) was isolated from a cDNA library from the brain of the frog, Bombina orientalis . (acs.org)
A human T cell-specific cDNA clone encodes a protein having extensive homology to immunoglobulin chains. (jax.org)
Interleukin-21 (IL-21) was first identified in 2000 through screening of a cDNA library from CD3+ human T cells [ 1 ]. (intechopen.com)
CD300d cDNA was cloned from RNA obtained from human peripheral blood mononuclear cells, and RT-PCR revealed the gene to be expressed in cells of myeloid lineage. (uab.cat)
The cloned cDNA encoded for a type I protein with a single extracellular Ig V-type domain and a predicted molecular mass of 21.5 kDa. (uab.cat)
Mouse and human cDNA clones encoding the ligand-binding IL-10 receptor (IL-10 R) have been isolated. (bio-techne.com)

KLRF1, an activating homodimeric C-type lectin-like receptor (CTLR), is expressed on nearly all natural killer (NK) cells and stimulates their cytoxicity and cytokine release (Kuttruff et al. (nih.gov)
IL-21R is a type-1 cytokine receptor and belongs to the IL-2R and IL-15R family. (intechopen.com)
IL-10 is also a pleiotropic cytokine with multiple immunostimulatory as well as immunosuppressive effects on a variety of other cell types. (bio-techne.com)
These receptors are members of the class II subgroup of the cytokine receptor superfamily. (bio-techne.com)

Having trained in the disciplines of immunology, molecular biology, and biotechnology, I have always been interested in applying state-of-the-art technologies to study human diseases and conditions. (stanford.edu)
Building on interrogations in basic T cell biology in the lab, bench-to-bedside translational immunology is being developed, with a focus on T cell-based therapy for ovarian cancer. (upenn.edu)

Combined with simple proliferative responses, the identified nucleotides are also shown to have functional consequences by inducing T cell proliferation for CD4/V β 20-1 + T cells, while failing to induce proliferation in other T cell isolates. (scirp.org)
Receptor tyrosine kinases (RTKs) play fundamental tasks in varied cell functions including proliferation differentiation survival migration and rate of metabolism (16). (exposed-skin-care.net)
Its roles include regulation of proliferation, promotion of immune system and activation of apoptosis in B cells. (intechopen.com)
In these cells, IL-21 does not promote proliferation but enhances apoptosis and chemotaxis. (intechopen.com)
Human bmMSCs were described in the late nineties as well [ 6 ] and at the same time a breakthrough study investigated the expression of typical cell surface markers and the proliferation and differentiation properties of human MSCs in more detail [ 7 ]. (hindawi.com)
Peripheral blood phenotyping data show increases in CD4+ and CD8+ T cell proliferation two weeks after anti-OX40 administration. (bepress.com)
These data provide evidence that anti-OX40 prior to surgery is safe and can increase activation and proliferation of CD4+ and CD8+ T cells in blood and tumor. (bepress.com)
This process is mediated by 2 mutually exclusive programs of gene expression: 1) an undifferentiated program supporting proliferation by stem cells within the basal layer and 2) a differentiation program instructing growth arrest and differentiation-associated programmed cell death in suprabasal layers. (stanford.edu)
First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. (hindawi.com)
ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
In response to mito- pendence between the key mecha- gens, cell proliferation is triggered by nistic characteristics. (who.int)
H - Ras cell proliferation are also linked with haematopoietic cel s. (who.int)

The flow cytometric dot plots show the correlated expression patterns of CD19 (Upper Panels) or CD14 (Lower Panels) versus Leptin Receptor (CD295) [or Ig Isotype control staining] for gated events with the forward and side light-scatter characteristics of viable mononuclear leucocytes. (bdbiosciences.com)
Expression of the platelet-derived growth factor alpha receptor (PDGF alpha R) is strictly regulated during mammalian development and tumorigenesis. (nih.gov)
fBB 4 -R expression in cells widely used for other Bn receptor subtypes was unsuccessful as was expression in two frog cell lines. (acs.org)
When genome editing induces a phenotypic alteration that is detectable by fluorescence (i.e., cell-surface expression of a target that can be non-lethally assessed with fluorescently labeled antibody), FACS provides a method of enriching edited cells 9 , narrowing the number of clones to propagate and analyze. (nature.com)
Three-color image analysis of F4/80 expression by cells in C57BL/6 mouse spleen. (bdbiosciences.com)
Moreover, the lack of a secreted form of the receptor was overcome by co-expression of hCG with the extra-cellular domain of porcine LH receptor leading to the secretion of a mixture of free as well as hCG-receptor complex [ 13 ]. (biomedcentral.com)
Additionally, the truncated Lhcgr, misrouted the full-length Lhcgr in ER, thereby decreasing the cell surface expression of the mature functional receptor. (biomedcentral.com)
instead, a specific intra-cellular role of truncated receptor in regulating the cell surface expression of a full-length ligand-binding Lhcgr has been proposed [ 15 ]. (biomedcentral.com)
The large-scale expression of many membrane proteins, including the members of the G protein-coupled receptor superfamily, in a correctly folded and fully functional form remains a formidable challenge. (essex.ac.uk)
First, we will outline the steps for establishing a tightly regulated gene expression system in human HEK293S cells. (essex.ac.uk)
Next, we describe the assembly of this expression system into HEK293S cells and a derivative cell line devoid of complex N-glycosylation. (essex.ac.uk)
The times at EMBL 'were amazing times' , making her return as a visiting scientist to work on 'the generation of new yeast expression vectors' in Gianni Cesareni's Lab after her further post-doctorial studies in Herman Bujard's Lab at Heidelberg University, where she 'engineered a new generation of cloning vectors' . (yale.edu)
It is involved in various metabolic processes, such as acting on white adipose tissue to upregulate brown adipose tissue-specific genes, enhancing PGC-1α expression to increase lipid oxidation, suppressing inflammation in skeletal muscles, inhibiting cardiometabolic risk factors, and suppressing endoplasmic reticulum stress in hepatoblastoma cells. (encyclopedia.pub)
Contrary to other activating members of the CD300 family of receptors, surface expression of CD300d in COS-7-transfected cells required the presence of an immunoreceptor tyrosine-based activating motif-bearing adaptor (FcεRγ). (uab.cat)
Our data suggest that the function of CD300d would be related to the regulation of the expression of other CD300 molecules and the composition of CD300 complexes on the cell surface. (uab.cat)
Restricted T cell receptor expression by human T cell clones specific for mycobacterial 65-kDa heat-shock protein: selective in vivo expansion of T cells bearing defined receptors. (ox.ac.uk)
Similar studies of TcR expression were carried out on hsp65-specific, DP4-restricted clones derived from the SF of a patient with rheumatoid arthritis by two independent cloning procedures. (ox.ac.uk)
In adapted cell lines exhibiting increased FGF2 and FGFR1 expression, measures of growth and signaling, but not EMT, were blocked by FGFR-specific TKIs, an FGF-ligand trap and FGFR1 silencing with RNAi. (refine.bio)
Epigenetic control of gene expression lasts through multiple cell divisions without alterations in primary DNA sequence and can occur via mechanisms that include histone modification and DNA methylation. (stanford.edu)
The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)

Resident memory (T RM ) cells are a distinct tissue-localized T cell lineage that is crucial for protective immunity in peripheral tissues. (frontiersin.org)
Following effective priming in lymph nodes, T cells traffic to tumors and other peripheral tissues. (frontiersin.org)
whereas T EM cells were shown to recirculate predominantly through blood, and peripheral tissues ( 10 ). (frontiersin.org)
Its pharmacology and cell biology are largely unknown, and no known natural cell lines or tissues possess sufficient numbers of fBB 4 -R's to allow either of these to be determined. (acs.org)
Placental hCG and pitutary LH transduce signals in target tissues through a common receptor (LHCGR). (biomedcentral.com)
NKp80 is a marker of natural killer (NK) cell maturity in secondary lymphoid tissues and supports NK cell development through a stage 4a intermediate with ILC3-associated features. (nih.gov)
Recently, MSCs isolated from bone marrow (bmMSCs) were shown to be a blend of distinct cells and MSCs isolated from different tissues show besides some common features also some significant differences. (hindawi.com)
2, 3 To test this hypothesis and account for potential nonspecific "bystander" T cells, 4 we examined the T-cell receptor (TCR) repertoire of matched human heart, tumor, and control tissues to identify T cells enriched in tumor or irMyocarditis. (bmj.com)
Across all patients, 17% (11/63) of tumor-enriched clones and 29% (11/37) of the irMyocarditis-enriched clones were enriched in both tissues. (bmj.com)
However, even though nearly 30 years have passed, in fish species, the distribution of GnRH-Rs in cells and tissues, their regulation, and their functions remain elusive. (biomedcentral.com)
C. Peterson, M. Ringne´r / Artificial Intelligence in Medicine 28 (2003) 59-74 structure and function, cellular metabolism, development of cells and tissues, and response of organisms to their environments. (lu.se)
Since genes are composed of a given alphabet (A, T, C, G) with fixed pairing properties A-T and C-G, probes can be constructed that attract gene transcripts extracted from cell tissues and cultures. (lu.se)
In mammals, physiological Wnt signaling is intimately involved with the biology of adult stem cells and self-renewing tissues (18,19). (hubrecht.eu)
These epithelial organoid cultures are genetically and phenotypically extremely stable, allowing transplantation of the cultured offspring of a single stem cell, as well as disease modeling by growing organoids directly from diseased patient tissues (32, 47, 53). (hubrecht.eu)
Skin malignancies, including epidermal squamous cell carcinoma (SCC), alone account for nearly as many cancers as all other tissues combined. (stanford.edu)
Both are effective at depleting B cells in blood, but these engineered CD19-targeted T cells can reach B cells sitting in tissues in a way that antibody therapies cannot, Konig explained. (medscape.com)
If you have a patient with myositis, for example, where autoreactive B cells are sitting in the inflamed muscle, or a patient with rheumatoid arthritis , where you have disease-relevant B cells in hard-to-reach tissues like the synovium, those cells are much harder to deplete with an antibody, compared to a T cell that evolved to surveil and effectively kill in all tissues," he explained. (medscape.com)
Another theory sug- perature, blood pressure, and noting signs gests that circulating immune complexes of liver cell failure such as pallor, jaundice, composed of HCV antigens and antibodies palmar erythema, fetor hepaticus and flap- deposit in tissues and initiate an inflamma- ping tremor. (who.int)

MIF binds to CD74, inducing its phosphorylation and the recruitment of CD44 which then activates non-receptor tyrosine kinases, leading ultimately to extracellular signal-regulated kinase phosphorylation. (wikipedia.org)
LHCGR recombinant proteins representing varying lengths of the N-terminal extracellular domain were expressed in Chinese Hamster Ovary cells in suspension culture. (biomedcentral.com)
GPCRs recognize a large variety of endogenous extracellular signaling molecules transmitting their corresponding signals inside the cell, and this process can be modulated by synthetic ligands or drug molecules. (nature.com)
Recombinant IL-10 soluble receptor, consisting of the extracellular domain of IL-10 R, binds IL-10 with high affinity in solution and is a potent IL-10 antagonist. (bio-techne.com)

The leucocytes were washed and stained with FITC Mouse Anti-Human CD14 (Cat No. 555397/557153/561712), BD Horizon™ V450 Mouse Anti-Human CD19 (Cat No. 644491) antibodies and either Alexa Fluor® 647 Mouse IgG2b, κ Isotype Control (Cat No. 557903) or Alexa Fluor® 647 Mouse Anti-Human Leptin Receptor (CD295) antibody (Cat No. 564376). (bdbiosciences.com)
Mouse anti PTEN tumour suppressor antibody, clone A2b1, is specific for PTEN, a tumour suppressor protein located on the human chromosome 10 that is often mutated in various types of advanced cancers. (bio-rad-antibodies.com)
Mouse anti Respiratory syncitial virus, major surface glycoprotein G antibody, clone 8C5 recognizes the major glycoprotein G of Respiratory Syncytial virus (RSV). (bio-rad-antibodies.com)
Here we describe the results from a phase Ib clinical trial (NCT02274155) in which 17 patients with locally advanced head and neck squamous cell carcinoma (HNSCC) received a murine anti-human OX40 agonist antibody (MEDI6469) prior to definitive surgical resection. (bepress.com)
The resultant DNA is placed in a mammalian cell culture, which then expresses the resultant gene, producing the desired antibody. (msdmanuals.com)

Engler, O.B., Strasser, I., Naisbitt, D.J., Cerny, A. and Pichler, W.J. (2004) A chemically inert drug can stimulate T cells in vitro by their T cell receptor in non-sensitised individuals. (scirp.org)
New model systems are being developed to test and optimize various immunotherapy strategies, including bispecific antibodies, checkpoint inhibitors, epigenetic modifiers and adoptive T cell transfer both in vitro and in vivo. (upenn.edu)
Soluble Axl (sAxl) is present in cell-conditioned medium of CUDC-305 (DEBIO-0932 ) tumor cells growing in vivo and in vitro and in the sera of humans mice and rats (23 50 However the identities of the sAxl-generating protease(s) and the mechanism(s) that account for this process remain unknown. (exposed-skin-care.net)
Expansion of MSCs was shown to be limited to a few passages of in vitro culture and the cells underwent replicative senescence [ 11 ]. (hindawi.com)
Changes in the differentiation potential of MSCs after in vitro expansion were noted and chondrogenic clones especially disappeared early on [ 12 ]. (hindawi.com)
Therefore, available in vitro protocols for expansion of MSCs do not yield true stem cells. (hindawi.com)
Lgr5 resides in Wnt receptor complexes and mediates signaling of the Wnt-agonistic R-spondins (31), explaining the unique dependence of Lgr5 stem cells on secreted R-spondins in vivo and in vitro. (hubrecht.eu)
The latter encompass human skin regenerated on immune deficient mice as well as organotypic constructs with epithelial and stromal cells embedded within architecturally faithful mesenchyma in vitro. (stanford.edu)
In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)

The hormonal activation occurs through specific ligand-receptor interactions on the surface of the target cells. (biomedcentral.com)

The earliest biochemical evidence on the existence of cell-free soluble LH receptor was the purification of an hCG-binding protein, relative molecular mass of 65K (M r , 65K) from porcine follicular fluid and was based on gel filtration followed by affinity chromatography [ 9 ]. (biomedcentral.com)
Therefore, how a complex LHCGR protein, embedded within the membrane lipid bilayer, could be secreted as soluble receptor remained unexplained. (biomedcentral.com)
Transient receptor potential canonical 6 (TRPC6) is a cation selective, DAG-regulated, Ca 2+ -permeable channel activated by the agonists of G q -protein-coupled heptahelical receptors. (mdpi.com)
Interferon gamma receptor 1 ( IFNGR1 ) also known as CD119 ( C luster of D ifferentiation 119 ), is a protein that in humans is encoded by the IFNGR1 gene . (wikidoc.org)
Finally, we describe methods for the growth of these cells lines in scalable suspension culture for the preparation of milligram amounts of recombinant protein. (essex.ac.uk)
Wucherpfennig, K.W. and Hafler, D.A. (1995) A review of T-cell receptors in multiple sclerosis: Clonal expansion and persistence of human T-cells specific for an immunodominant myelin basic protein peptide. (scirp.org)
Human apelin was identified and isolated in 1998 as an endogenous ligand of the G-protein-coupled receptor APJ and was named the APJ endogenous ligand. (encyclopedia.pub)
The complex pharmacology of G-protein-coupled receptors (GPCRs) is defined by their multi-state conformational dynamics. (nature.com)
One cryoEM and over 50 high-resolution X-ray crystallographic structures are available for antagonist- or agonist-bound A 2A AR and for its ternary complex with an agonist and an engineered G protein, making this receptor an excellent model system for investigating GPCR structural dynamics. (nature.com)
A common heterophilic ligand for these RTK family members is definitely Gas6 a vitamin K-dependent protein that is widely secreted by most cells including the lungs intestine Serping1 and vascular endothelium (43). (exposed-skin-care.net)
Gas6 is the product of growth arrest-specific gene 6 which was in the beginning cloned from serum-starved fibroblasts and shares about CUDC-305 (DEBIO-0932 ) 44% sequence identity and related website organization with protein S a negative regulator of blood coagulation (48). (exposed-skin-care.net)
PTEN exhibits protein phosphatase activity and can suppress the growth of glioma cells. (bio-rad-antibodies.com)
In addition to previously observed resistance mechanisms including EGFR-T790M ''gate-keeper'' mutations and MET amplification, a subset of the seven chronically adapted NSCLC cell lines including HCC4006, HCC2279 and H1650 cells exhibited marked induction of fibroblast growth factor (FGF) 2 and FGF receptor 1 (FGFR1) mRNA and protein. (refine.bio)
DeMarini, and Chapter 20, by Rice and cell death determine the size protein in several signal ing path- and Herceg). (who.int)

We demonstrate that recombinant LHCGR proteins which include the hormone-binding domain are secreted from transfected cells and that natural LHCGR is also secreted from human placental explants. (biomedcentral.com)
2013) The molecular basis for Mucosal-Associated Invariant T cell recognition of MR1 proteins. (scirp.org)
Myokines are small proteins (5-20 kDa) and proteoglycan peptides that are produced and secreted by skeletal muscle cells in response to muscle contractions. (encyclopedia.pub)
Several studies shown that Axl could mediate cell adhesion and aggregation through homotypic ectodomain associations (9 23 Both CUDC-305 (DEBIO-0932 ) murine and human being Axl proteins undergo proteolytic processing to yield a soluble form of this molecule. (exposed-skin-care.net)
Ectodomain dropping has emerged as an important posttranslational mechanism to regulate the functions of various integral membrane-bound proteins including adhesion molecules cytokines growth factors and their receptors (57 60 Both. (exposed-skin-care.net)

The circulating MIF binds to CD74 on other immune cells to trigger an acute immune response. (wikipedia.org)
Furthermore, glucocorticoids also stimulate white blood cells to release MIF and hence MIF partially counteracts the inhibitory effects that glucocorticoids have on the immune system. (wikipedia.org)
While a great deal of effort has focused on defining their role in immunity to infections, studies now reveal T RM cells as a vital component of the host immune response to cancer. (frontiersin.org)
Cancer can be considered a disease of immune dysfunction, with a failure of immune recognition leading to the outgrowth of malignant cells as tumors ( 1 ). (frontiersin.org)
Tumor development has been said to occur in three distinct steps: inefficient elimination of early transformed cells, development of a state of equilibrium between tumor cells and immune cells, and tumor escape from immune pressure ( 2 ). (frontiersin.org)
The recent success of T cell immune checkpoint inhibitor (ICI) therapies for cancer has revealed CD8 T cells as potent mediators of immunity against advanced cancers ( 4 , 5 ). (frontiersin.org)
One focus of the Powell lab is on the generation/isolation high avidity, tumor-reactive T cells from heterogenous tumor infiltrating lymphocyte populations in traditionally "non-immunogenic" cancers utilizing novel T cell capture techniques, and to deeply examine the characteristics and function of these immune cells. (upenn.edu)
One approach relies on the isolation and cloning of T cell receptors (TCRs) that confer non-reactive T cells with specific and potent immune function following gene transfer. (upenn.edu)
Other current efforts include the exploration of immunomodulation to potentiate endogenous antitumor T cell responses, use of bispecific antibodies, pharmacological sensitization of tumor cells to immune attack, tumor vasculature targeting, preclinical validations, clinical translation and trial support. (upenn.edu)
This position is an extension of her Research in molecular biology, encompassing and combining her passion to further develop the mechanisms by which the immune system regulates the balance between protective immunity and immune-driven pathologies. (mdanderson.org)
Tissue, even entire organs that are transplanted have normal cell markers, but when they aren't your own, they cause the immune system to attack the tissue - this is why people receiving transplants need to take immunosuppressants. (scienceaid.net)
and suppressor T cells that slow down the immune response about 1 week after the response. (scienceaid.net)
Overview of the Immune System The immune system distinguishes self from nonself and eliminates potentially harmful nonself molecules and cells from the body. (msdmanuals.com)

Human dNK cell function is differentially regulated by extrinsic cellular engagement and intrinsic activating receptors in first and second trimester pregnancy. (nih.gov)
Therefore, smFRET has been limited to studies of inter-receptor interactions in cellular membranes and receptors in detergent environments. (nature.com)
Using mosaic analysis and RNAi in the model organism Drosophila melanogaster, we show that Dystroglycan is required cell-autonomously for cellular polarity in two different cell types, the epithelial cells (apicobasal polarity) and the oocyte (anteroposterior polarity). (lu.se)

Indeed a wealth of studies from humans and mouse models establishes a particularly potent role for CD8 T cells in controlling the outgrowth of malignancies ( 3 ). (frontiersin.org)
2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)

Characterized by cell-surface molecules including CD103, CD69, and CD49a, T RM -like tumor-infiltrating lymphocytes (TILs) can be found in a wide range of human cancers, where they portend improved prognosis. (frontiersin.org)
Here, we performed smFRET experiments on functionally active human A 2A adenosine receptor (A 2A AR) molecules embedded in freely diffusing lipid nanodiscs to study their intramolecular conformational dynamics. (nature.com)
These three phenomena indicate that receptors are highly dynamic molecules and sample several active and inactive states stochastically (for review, see refs. (nature.com)
Cells have molecules in their wall that identifies them. (scienceaid.net)
MW) chemicals interact with host cells and molecules and In our daily life, we are confronted with a plethora of natural influence biologic processes such as signal transduction. (cdc.gov)

This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. (wikipedia.org)
This review will recount the path to discovering the importance of resident memory CD8 T cells as they pertain to cancer immunity. (frontiersin.org)
In addition to highlighting key studies that directly implicate T RM cells in anti-tumor immunity, we will highlight earlier work that implicitly suggested their importance. (frontiersin.org)
These early classifications of T cell memory were quickly brought to bear on the question of what T cell subset provides the best immunity against cancer. (frontiersin.org)
OX40, GITR, and 4-1BB), can enhance T cell immunity in tumor-bearing hosts. (bepress.com)
1 Reports of shared T-cell clones in irAE and tumor suggest that common antigens or epitopes may couple anti-tumor immunity and irAE biology. (bmj.com)
Conclusions TCRs enriched in ICI-treated tumors and irMyocarditis heart tissue are often distinct, suggesting that T-cell responses causing severe irAEs and anti-tumor immunity may not be driven by common antigens or epitopes, as has been previously suggested in other settings. (bmj.com)
Differentiating the T-cells responses driving irMyocarditis and anti-tumor immunity may be the first step towards developing clinical strategies that can maximize ICI efficacy while mitigating irAEs. (bmj.com)

Here, we establish a method to enable clonal isolation, selection, and expansion of properly edited cells, using OptoElectroPositioning technology for single-cell manipulation on a nanofluidic device. (nature.com)
Each colony is split and sequentially exported for on-target sequencing and further off-chip clonal expansion of the validated clones. (nature.com)
Long-term clonal culturing of organoids from Lgr5 stem cells. (hubrecht.eu)

Multicolor flow cytometric analysis of Leptin Receptor (CD295) on human mononuclear cells. (bdbiosciences.com)
This study describes the molecular cloning of the non-coding exon 1 and approximately 2 kb of 5' flanking region of the human PDGF alpha R gene. (nih.gov)
In addition, the morphogen retinoic acid, alone or in combination with dibutyryl cAMP, gives a 22-fold induction of PDGF alpha R gene promoter activity in human teratocarcinoma cells. (nih.gov)
NKp80 Defines a Critical Step during Human Natural Killer Cell Development. (nih.gov)
Identification of NKp80, a novel triggering molecule expressed by human NK cells. (nih.gov)
The human interferon-gamma receptor. (wikidoc.org)
Komatsu, T., Moriya, N. and Shiohara, T. (1996) T cell receptor (TCR) repertoire and function of human epidermal T cells: Restricted TCR V alpha-V beta genes are utilized by T cells residing in the lesional epidermis in fixed drug eruption. (scirp.org)
1998) Comparative sequence analysis of the human T cell receptor beta chain in juvenile rheumatoid arthritis and juvenile spondylarthropathies: Evidence for antigenic selection of T cells in the synovium. (scirp.org)
We therefore briefly discuss differences found in subsets of human bmMSCs and in MSCs isolated from some other sources and touch upon how this could be utilized for cell-based therapies. (hindawi.com)
Human and mouse IL-10 receptors are structurally related to the IFN-gamma receptor. (bio-techne.com)
The deduced amino acid sequence of human IL-10 R is approximately 60% identical to mouse IL-10 R. Although human IL-10 has cross-species activities and is active on mouse cells, mouse IL-10 is species-specific in its actions and does not bind to the human IL-10 receptor. (bio-techne.com)
Mouse anti-human CD45-FITC (Clone 2D1, Cat No. 347463), Mouse Anti-human CD34-PE [Clone 8G12 (also known as HPCA2), Cat No. 348057], Mouse anti-human CD38-PE-Cy™5 (Clone HIT2, Cat No. 555461) and appropriated isotype control antibodies were purchased from BD Biosciences (San Diego, CA, USA). (researchsquare.com)
Based on these combined insights, we have established Lgr5/R-spondin-based culture systems that allow the outgrowth of single mouse or human Lgr5 stem cells into ever-expanding organoids. (hubrecht.eu)
Our experimental focus is on the mammalian setting using multiomics, informatics, mouse genetics, human genetics, single cell studies, and new human tissue platforms. (stanford.edu)
The Viral Brain Disease Activated By Monoclonal Antibodies reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (prionics.com)
Description: A competitive ELISA for quantitative measurement of Human Platelet antibodies IgG in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. (prionics.com)
Fully human monoclonal antibodies are produced using transgenic mice that contain human immunoglobulin genes or using phage display (ie, a bacteriophage-based cloning method) of immunoglobulin genes isolated from human B cells. (msdmanuals.com)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

In this study, we focused on measuring LSCs easily in the bone marrow cells from AML patients by developing a four-color flow cytometric analysis. (researchsquare.com)

Another field of study is the de novo generation of tumor-reactive T cells through genetic engineering methods. (upenn.edu)
e.g. inflammation, genotoxicity, and This chapter focuses on issues as- ellers, growth factors, growth factor epigenetic alterations) and can have sociated with the understanding and receptors, signal transducers, and both genetic and epigenetic origins. (who.int)

They display differentiation capacities and therefore qualify as multipotent progenitor cells (Figure 1 ). (hindawi.com)
Overview on self-renewal or differentiation of stem cells in their respective stem cell niche. (hindawi.com)
In stratified epithelia proliferative basal cells adherent to the underlying basement membrane undergo cell cycle arrest then outward migration and terminal differentiation. (stanford.edu)
We are currently pursuing studies of the dominant signaling and gene regulatory networks that control this process, including the Ras/MAPK cascade, which is required for stem cell-mediated self-renewal and the p53 transcription factor family member, p63, which is required for epidermal differentiation. (stanford.edu)
The function of histone modifying epigenetic regulators and noncoding RNA as central mediators of epithelial stem cell renewal and differentiation represent major emerging areas of study in the lab. (stanford.edu)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

Today, more than 50 years after the first successful bone marrow transplantation, clinical application of hematopoietic stem cells is a routine procedure, saving the lives of many every day. (hindawi.com)
However, transplanting other than hematopoietic stem and progenitor cells is still limited to a few applications, and it mainly applies to mesenchymal stromal cells (MSCs) isolated from bone marrow. (hindawi.com)
The MSCs have been described for the first time as colony forming fibroblasts (CFU-F), a rare population of cells residing in the bone marrow of guinea-pigs or mice [ 1 , 2 ]. (hindawi.com)
Nowadays, experts agree that MSCs may generate upon appropriate stimulation quite different mature cells including osteoblasts, chondrocytes, tenocytes, adipocytes, smooth muscle cells, and stromal cells of the bone marrow [ 9 ]. (hindawi.com)
They are made from stem cells in the bone marrow (soft tissue in bones). (scienceaid.net)
B lymphocytes are also made in the bone marrow and mature in lymph nodes where they acquire membrane receptors. (scienceaid.net)
We examined the CD45 dim CD34 + CD38 - CD133 + cells on bone marrow samples of hematologic malignancies and healthy controls using four-color flow cytometry experiments. (researchsquare.com)
Interestingly, the CD45 dim CD34 + CD38 - CD133 + cells were highly expressed in bone marrow of patients with AML compared to that of healthy controls (HC). (researchsquare.com)

However, stable fBB 4 -R cell lines were obtained in CHO-K1 cells which were shown to faithfully demonstrate the correct pharmacology of the related Bn receptor, the GRP receptor, when expressed in these cells. (acs.org)

Watkins, S.L. and Pichler, W.J. (2013) Activating interactions of sulfanilamides with T cell receptors. (scirp.org)
The molecular interactions of genes and gene products underlie fundamental questions of biology. (lu.se)

Bacterial antigens stimulate white blood cells to release MIF into the blood stream. (wikipedia.org)

Second, different agonists vary in efficacy and can stimulate receptor activity to a different extent 5 . (nature.com)

A large number of cloned B cells secrete antibodies that kill the microorganism. (scienceaid.net)
The authors described seroconversion of antinuclear antibodies in two patients with the longest follow-up, "indicating that abrogation of autoimmune B-cell clones may lead to a more widespread correction of autoimmunity," the researchers write. (medscape.com)

Yes, I worked with Gail Martin on embryonic stem cells and then returned to Harvard in 1986. (medscape.com)

At the same time, by asymmetric cell division or after specific activation, stem cells must be able to generate more mature progenitor cells or differentiated effector cells (Figure 1 ). (hindawi.com)
Moreover, the CD34 + CD38 - progenitor cells expressed variable amounts of the target receptor CD33, CD133 and c-kit (CD117) [ 20 ]. (researchsquare.com)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

Such tumor-specific T RM cells are recognized as both necessary and sufficient for long-lived protection against tumors in peripheral tissue locations. (frontiersin.org)
In a growing number of cases, CD8 T cells have been shown to mediate the regression of large bulky tumors, resulting in durable long-term disease remissions ( 5 ). (frontiersin.org)
diagnostic prediction of small round blue cell tumors (SRBCT) of childhood and determining the estrogen receptor (ER) status of sporadic breast cancer. (lu.se)
Despite initial and often dramatic responses of epidermal growth factor receptor (EGFR)-addicted lung tumors to the EGFR-specific tyrosine kinase inhibitors (TKIs), gefitinib and erlotinib, nearly all develop resistance and relapse. (refine.bio)
Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)

Structurally, LHCGR is similar to thyroid and follicle stimulating hormone receptors (TSHR and FSHR respectively). (biomedcentral.com)

Genomic Deletion, purified, cloned, and sequenced PCR products on an au- tomated DNA sequencer by using T7, SP6 primers, and South Korea newly designed S gene-specific primers (primer sequences available on request). (cdc.gov)
Over the course of his 30-plus-year career, George Church has pioneered not one, but several transformative fields in medicine, including genomic sequencing, synthetic biology, and, most recently, genome engineering. (medscape.com)

DPhe 6 ,βAla 11 ,Phe 13 ,Nle 14 ]Bn(6−14) was found to have high affinity ( K i = 0.4 nM) for the fBB 4 receptor and 125 I-[DTyr 6 ,βala 11 ,Phe 13 ,Nle 14 ]Bn(6−14) to be an excellent ligand for this receptor. (acs.org)
IL-10 binds specifically and with high affinity to cell-surface receptors. (bio-techne.com)

IL-17a-producing gammadeltaT cells and NKG2D signaling mediate bacterial endotoxin-induced neonatal lung injury: implications for bronchopulmonary dysplasia. (jax.org)
2007) Structural basis of T-cell specificity and activation by the bacterial superantigen TSST-1. (scirp.org)

The control of this transition from epithelial stem cell to differentiated corneocyte, which is abnormal in epidermal cancers, is not well understood. (stanford.edu)

MSCs were also investigated for stem cell qualities in vivo. (hindawi.com)

Merging computational and simple cell assays, this work establishes a role of nucleotides in T cells found to contain this TCR subtype. (scirp.org)

Upon immobilization sAxl advertised cell migration and induced the phosphorylation of Axl and phosphatidylinositol 3-kinase. (exposed-skin-care.net)

The signaling mechanisms of IL-21 in different cell types have been identified. (intechopen.com)
However, we know less about the biological effects of IL-21 and its signaling mechanisms in leukemia cells and monocytes. (intechopen.com)
In this chapter, we will focus on IL-21's biological effects and signaling pathways as well as discuss the potential implications and applications of IL-21 in leukemia cells. (intechopen.com)
We were the first to link Wnt signaling with adult stem cell biology, when we showed that TCF4 gene disruption leads to the abolition of crypts of the small intestine (8), and that TCF1 gene knockout severely disables the stem cell compartment of the thymus (2). (hubrecht.eu)

Interleukin-21 (IL-21) is produced by activated T cells and it plays many diverse roles by regulating the functions of normal and abnormal cells. (intechopen.com)

Selection of edited cell clones currently relies on limiting dilution or fluorescence-activated cell sorting (FACS)-based single-cell sorting to isolate single cells. (nature.com)
fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)

There was a close correlation between agonist the receptor occupation and the receptor activation. (acs.org)
When stimulated by agonists of the histamine H1 receptor (H1R), TRPC6 activity decays to the baseline despite the continuous presence of the agonist. (mdpi.com)

Loss of Dystroglycan function in follicle and disc epithelia results in expansion of apical markers to the basal side of cells and overexpression results in a reduced apical localization of these same markers. (lu.se)
Dystroglycan is also required non-cell-autonomously to organize the planar polarity of basal actin in follicle cells, possibly by organizing the Laminin ECM. (lu.se)

Here, Dr. Pauken made the seminal observation that T cell exhaustion represents its own unique cell state, fundamentally different at the epigenetic level than functional effector or memory cells that develop during acute infection. (mdanderson.org)

In 1991, we reported the cloning of a T cell specific transcription factor that we termed TCF1 (1). (hubrecht.eu)

11 ] first reported the production and secretion of soluble LH receptor following transient transfection of a naturally truncated variant of the receptor in COS cells. (biomedcentral.com)

1994) T cell receptor V alpha repertoire of infiltrating T cells in labial salivary glands from patients with Sjogren's syndrome. (scirp.org)
1992) T cell receptor repertoire of infiltrating T cells in lips of Sjogren's syndrome patients. (scirp.org)

A type of T cell called killer T lymphocytes bind to the pathogen and produce cytotoxin that destroys cells. (scienceaid.net)
Cutaneous T-cell lymphoma (CTCL) (see the image below) is a heterogeneous group of lymphoproliferative disorders characterized by localization of neoplastic T lymphocytes to the skin, with no evidence of extracutaneous disease at the time of diagnosis. (medscape.com)

and biomedicine and Cancer biology, Molecular Biology small molecule drug discovery experience working in a new hybrid multiinstitutional model adopting the bench at bedside approach. (mdanderson.org)
Unexpectedly, we could not detect CD300d on the surface of cells expressing FcεRγ, suggesting the existence of unknown mechanisms regulating the trafficking of this molecule. (uab.cat)
The targeting of oncogenic 'driver' kinases with small molecule inhibitors has proven to be a highly effective therapeutic strategy in selected non-small cell lung cancer (NSCLC) patients. (refine.bio)

Such memory T cells were traditionally defined as being comprised of both central memory (T CM ) and effector memory (T EM ) subsets ( 9 ). (frontiersin.org)
rather, these inhibitors temporarily boosted effector activity of genes that were confined to the open chromatin landscape within exhausted T cells. (mdanderson.org)

Leukemia stem cells (LSCs) in acute myeloid leukemia (AML) played important roles in development of leukemia, chemotherapeutic drug resistance, and disease relapse and progression. (researchsquare.com)
Acute myeloid leukemia (AML) is generally regarded as a stem cell disease, and it originates from a hierarchy of leukemic stem cell classes that differ in self-renewal capacity [ 1 , 2 ]. (researchsquare.com)

These cells represent the epithelial stem cells of the small intestine and colon (23), the hair follicle (24), the stomach (28) and many other tissue stem cell types. (hubrecht.eu)

Recent studies in mouse tumor models have shown that T RM cells are induced by cancer vaccines delivered in peripheral tissue sites, or by the depletion of regulatory T cells. (frontiersin.org)
As the field of cancer immunotherapy advances rapidly, it is now crucial to understand how the dissemination and maintenance of tumor-specific T cells can be optimally achieved. (frontiersin.org)
In 2016, Dr. Pauken then went on to study in the lab of Dr. Arlene Sharpe at Harvard Medical School, where she focused on mechanisms of PD-1-mediated regulation of CD8 + T cells in cancer. (mdanderson.org)
Dr Datar has 18 yrs. of hands-on experience involving Molecular/Cancer biology, clinical data mgmt. (mdanderson.org)
In the Pauken lab, Dr. Datar seeks to continue her work in the field of Immunotherapy and Cancer biology. (mdanderson.org)
Results Four tumor histologies were captured: melanoma (n=2), renal cell carcinoma, cholangiocarcinoma, and prostate cancer. (bmj.com)
The Organoid group, previously Clevers group, studies the molecular mechanisms of tissue development and cancer of various organs using organoids made from adult Lgr5 stem cells. (hubrecht.eu)
In APC-deficient colon carcinoma cells, we demonstrated that ß-catenin accumulates and is constitutively complexed with the TCF family member TCF4, providing a molecular explanation for the initiation of colon cancer (5). (hubrecht.eu)
The Tcf4-driven target gene program in colorectal cancer cells is the malignant counterpart of a physiological gene program in selfrenewing crypts (13, 14). (hubrecht.eu)
Activating transcription factor-(ATF-) 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. (hindawi.com)
In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)

2,3 We are currently analyzing these data together with our unpublished single-cell RNA sequencing and TCR data from peripheral blood and heart, which encompass 25 patients and ~300,000 cells. (bmj.com)
DR3-restricted clones were raised from both the synovial fluid (SF) and peripheral blood (PB) of a patient with reactive arthritis in three separate cloning events. (ox.ac.uk)

Subtle differences in epitope specificity were detected for two clones with differing TcR. (ox.ac.uk)
Once more, T cell clones with identical alpha and beta TcR chains were obtained from the separate cloning procedures, suggesting oligoclonalty of T cells with this defined specificity in the patient's SF. (ox.ac.uk)

June and his research team led the development of CAR T-cell therapies for blood cancers. (medscape.com)

Anatomy45

Organisms27

Diseases3

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Psychiatry and Psychology5

Phenomena and Processes61

Disciplines and Occupations21

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture7

Humanities4

Information Science9

Named Groups1

Health Care2

Antigen24

CDNA6

Cytokine4

Immunology2

Proliferation12

Expression20

Tissues18

Extracellular4

Antibody5

Vitro9

Ligand-receptor1

Protein14

Proteins5

Immune13

Cellular3

Humans2

Molecules5

Immunity8

Clonal3

Human19

Flow cytometric analysis1

Genetic2

Differentiation6

Bone marrow8

Pharmacology1

Interactions2

Antigens1

Stimulate1

Antibodies2

Embryonic Stem1

Progenitor cells3

Tumors5

Structurally1

Genomic2

High-affinity2

Bacterial2

Epidermal1

Vivo1

Subtype1

Phosphorylation1

Signaling4

Interleukin1

Fluorescence-activated cell2

Agonist2

Basal2

Functional1

19911

Transient1

Repertoire2

Lymphocytes2

Molecule3

Effector2

Myeloid2

Follicle1

Cancer11

Peripheral blood2

Specificity2

Therapies1