• Application of various small molecules and recombinant proteins to mouse embryonic stem cells at specific time points in vitro has enabled recapitulation of developmental cues with subsequent formation of inner ear organoids. (springer.com)
  • These results show that human brain organoids can be applied as an in vitro model for CNS drug screening to evaluate structural, cellular, and molecular changes in the normal brains or brains of patients with neuropsychiatric disorders after drug treatments. (frontiersin.org)
  • The cerebral organoids, which can mimic the endogenous development of human brain, have been applied to different studies as in vitro models for a wide range of brain diseases ( 16 , 21 , 22 ). (frontiersin.org)
  • Intestinal organoids are three-dimensional (3D) in vitro tissue cultures that model the in vivo intestine. (stemcell.com)
  • To find out, Stachowiak and colleague and spouse, Ewa Stachowiak, assistant professor of pathology and anatomical sciences, adapted mini-brain technology, growing in vitro miniature brain structures called cerebral organoids. (scienceblog.com)
  • The requirements for the successful culture of organoids in vitro differ significantly from those of traditional monolayer cell cultures. (mdpi.com)
  • The use of 3-dimensional (3D) in vitro models to more faithfully recapitulate in vivo tumour biology within the laboratory is accelerating rapidly as a result of advances in 3D bioprinting, tissue organoids, microfluidic and automated high content confocal screening technologies. (ed.ac.uk)
  • This failure of the preclinical pipeline highlights the need for improved, physiologically-relevant in vitro models that can better serve as reliable drug-screening and disease modeling tools. (aracaribio.com)
  • Since models are limited to study the mechanism of the neurotoxicity of tranylcypromine, here we utilized an in vitro brain organoid culture system which mimics neurogenesis experimentally to investigate the mechanisms by studying the effect of brain development of tranylcypromine and monitoring the brain damage of different concentrations. (frontiersin.org)
  • This 2-hit hypothesis is supported by our in vitro models using isolated human endometrial cells, which following TCDD exposure, exhibit an enhanced response to an inflammatory challenge related to infection (e.g. (vanderbilt.edu)
  • Some of these techniques include novel tools for genetic manipulation, 4,5 approaches for in vitro disease modelling 6-9 and innovative co-culture system with autologous cell types 10,11 or bacteria, 12-14 as well as viral infection models. (stemcell.com)
  • His work utilizes a systems biology, molecular virology, and comparative pathogenesis approaches which utilize in vitro and in vivo models which accurately recapitulate human disease. (utmb.edu)
  • Usually cancer is studied either using in vitro static models or in vivo animal models. (youris.com)
  • It is difficult to establish adequate in vitro models for pathological analysis because of the heterogeneity of these disorders. (nih.gov)
  • In the present study, to recapitulate the pathologies of these disorders in vitro , we established in vitro models by differentiating mature neurons from human induced pluripotent stem cells (hiPSCs) derived from BP and SCZ patient with contributive copy number variations, as follows: two BP patients with PCDH15 deletion and one SCZ patient with RELN deletion. (nih.gov)
  • These results suggest that the observed phenotypes are general phenotypes of psychiatric disorders, and our in vitro models using hiPSC-based technology may be suitable for analysis of the pathologies of psychiatric disorders. (nih.gov)
  • More complex cancer in vitro models have been developed, but they still lack organ-level structures, fluid flows, and mechanobiological cues that cells experience in vivo. (cancer.gov)
  • METHODS: To study the complexity of the mechanisms involved in the resistance to the selective estrogen receptor degrader (SERD) fulvestrant, we performed comprehensive biomarker analyses using several in vitro models that recapitulate the heterogeneity of developed resistance. (lu.se)
  • RESULTS: We found that different in vitro models of fulvestrant resistance show variable stability in their phenotypes, which corresponded with distinct genomic alterations. (lu.se)
  • The models recapitulate many of the features of bone marrow metastatic disease in neuroblastoma patients. (lu.se)
  • We are continuously evaluating new treatments in our clinically relevant, patient-derived models in vitro and in vivo . (lu.se)
  • Implement an in vitro, microscaled, living, instrumented fetal membrane-on-a-chip (IFMOC) that recapitulates the physiological properties of the human tissue. (vanderbilt.edu)
  • Accurate biological screening based on real human intestinal tissue, with a standardized workflow for reliable and reproducible data, RepliGut ® represents the next generation for in vitro testing with faster actionable data. (drugdiscoveryonline.com)
  • An organoid is a 3D organization of cells that can recapitulate some of the structure and function of native tissue. (mdpi.com)
  • Indeed, due to the endogenous nature of the stroma, the model proposed could represent a valuable tool to in vitro study tissue status at both cellular and extracellular level after a physical damage. (unina.it)
  • When human serum was used for building 3D engineered bone, the tissue recapitulated bone homeostasis and response to bisphosphonates observed in native bone. (ibecbarcelona.eu)
  • Here, we investigated the role of the n-3 PUFA docosahexaenoic acid (DHA) as a component of n-6 to n-3 PUFA mixtures used to recapitulate physiologically relevant dietary ratios in the resolution of inflammatory injury caused by exposure to EVs carried by agricultur al organic dust in vitro. (cdc.gov)
  • This chapter highlights the process of in vivo inner ear development, the rationale and process behind inner ear organoid formation, and potential applications and limitations of this in vitro model system. (springer.com)
  • A brain organoid is a cell aggregate formed by recapitulating the development processes of the fetal brain in vitro. (fujipress.jp)
  • Thermal stability assays show that ERKi do not destabilise ERK2 (or ERK1) in vitro, suggesting that ERK2 turnover is a cellular consequence of ERKi binding. (babraham.ac.uk)
  • These results underscore the importance of n-3 PUFA as anti-inflammatory compounds during recovery from EV-laden environmental dust exposure in the context of cellular responses in vitro, warranting future translational studies. (cdc.gov)
  • More striking, the pancreas in these mice appeared relatively normal, with well-demarcated islets, suggesting that Pax4 misexpression was inducing an orderly and spatially correct program of cellular conversion that probably recapitulated the normal cellular differentiation process. (medscape.com)
  • A patterned neural tube and somites in an embryonic context are generated in vitro. (nature.com)
  • Embryonic blood cell development occurs via well-defined developmental stages which are recapitulated in vitro by differentiation of embryonic stem cells. (nih.gov)
  • Defective remyelination can be recapitulated in vivo in mice receiving systemic administration of pharmacological HDAC inhibitors during cuprizone treatment and is consistent with in vitro results showing defective differentiation of oligodendrocyte progenitors after silencing specific HDAC isoforms. (nih.gov)
  • Culture media containing human serum enhanced osteogenesis and osteoclasts differentiation, and provided a more realistic in vitro mimic of human cancer cell proliferation. (ibecbarcelona.eu)
  • By stimulating hair cells with sinusoidal voltage commands that mimic pure sound tones, we recapitulated the phase-locking of hair cell exocytosis with an in vitro approach. (jneurosci.org)
  • We showed that these effects were recapitulated in vitro by increasing endogenous UCP2 expression using saRNA. (aacrjournals.org)
  • This antibody blocks RAGE signaling in vitro and in vivo, recapitulating the phenotype of Ager AHA/AHA mice. (elifesciences.org)
  • Targeting the conditional Rlim cKO specifically to the spermatogenic cell lineage largely recapitulates this phenotype. (umass.edu)
  • However, both approaches fail to recapitulate the metastatic process for different reasons. (youris.com)
  • 2D monolayer cultures fail to recapitulate the totality of the tumor microenvironments. (cancer.gov)
  • Additionally, she developed an in vitro model of early human neurulation events. (wikipedia.org)
  • The vascularized micro-tumor (VMT) is a novel three-dimensional model system (tumor-on-a-chip) that recapitulates the complex human tumor microenvironment, including perfused vasculature, within a transparent microfluidic device, allowing real-time study of drug responses and tumor-stromal interactions. (aracaribio.com)
  • Compared with conventional mice model, this model can recapitulate the human brain development in vitro ( 20 ). (frontiersin.org)
  • We have recently recapitulated the segmentation clock, oscillatory gene expression during early development, from human and mouse pluripotent stem cells. (irbbarcelona.org)
  • Here, we report the development and applicability of a functional neurovascular unit on a microfluidic chip as a microphysiological model of ischaemic stroke that recapitulates the function of the blood-brain barrier as well as interactions between therapeutic stem cells and host cells (human brain microvascular endothelial cells, pericytes, astrocytes, microglia and neurons). (stanford.edu)
  • To recapitulate in vitro the features of human bone and bone cancer, we investigated the effects of human serum and human platelet lysate on modeling osteogenesis, osteoclastogenesis, and bone cancer in two-dimensional (2D) and three-dimensional (3D) settings. (ibecbarcelona.eu)
  • Here, we have developed a human in vitro model that recapitulates key aspects of dopaminergic innervation of the striatum and cortex. (lu.se)
  • Our findings were subsequently tested by in vitro experiments, where putative early and later-stage DN1 progenitors from the thymus were purified and their pro- gression into DN2 was measured. (lu.se)
  • This information will be used to recapitulate similar conditions in vitro and induce the growth of hematopoietic stem cells outside the body from adult endothelial cells. (ca.gov)
  • The laboratory mice, in terms of metastasis, do not always recapitulate what we see in the patients. (youris.com)
  • In vitro fertilization is a commonly used technique with a variety of model organisms to maintain lab populations and produce synchronized embryos for downstream applications. (jove.com)
  • Finally, we demonstrate how to produce viable offspring using in vitro fertilization and discuss signs of successful fertilization. (jove.com)
  • Systemic deletion of the Rlim gene results in lower numbers of mature sperm that contains excess cytoplasm, leading to decreased sperm motility and in vitro fertilization rates. (umass.edu)
  • The UB approach modifies a recently developed protocol for developing early brain structures in vitro. (scienceblog.com)
  • To gain insights into the mechanisms driving astrocyte maturation, we have characterised acutely isolated murine astrocytes at both postnatal and adult stages, as well as in vitro differentiated astrocytes. (nature.com)
  • The goal was to, in a sense, recapitulate important stages in brain formation that take place in the womb," Stachowiak says. (scienceblog.com)
  • In order to understand how these processes are regulated and coordinated by FERARI, we aim to biochemically elucidate and recapitulate FERARI function in vitro. (unibas.ch)
  • If successful, the B2B device will be an in vitro alternative that features three new complementary technologies. (youris.com)
  • The investigators showed that a class of antimalarial drugs known as artemisinins, typified by the FDA-approved drug artemether , were capable of inhibiting ARX levels, reducing glucagon, and increasing insulin in an in vitro alpha-cell model. (medscape.com)
  • Investigating astrocyte maturation in a cell culture model revealed that in vitro-differentiated astrocytes lack expression of many mature astrocyte-specific genes, including genes for the transcription factors Rorb, Dbx2, Lhx2 and Fezf2. (nature.com)
  • This model was composed by a cell-synthesized and responsive extracellular matrix that resembles the in vivo dermis, and it was used as living platform to study in vitro skin alteration and diseases. (unina.it)
  • An integrated approach of in vitro, ex vivo, in silico and in vivo studies is key to enable acceleration of availability of pharmacology data in pregnancy and lactation. (springer.com)
  • An integrated approach utilizing multiple novel methodologies (in vitro, ex vivo, in silico and in vivo) is needed to accelerate the availability of pharmacology data in pregnancy and lactation. (springer.com)
  • To study differences in time between species, my group uses in vitro systems. (irbbarcelona.org)
  • Forced expression of these factors in vitro induces distinct sets of mature astrocyte-specific transcripts. (nature.com)