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MutagenesisMutagenesis, Site-DirectedIndicators and ReagentsMutagenesis, InsertionalMolecular Sequence DataSulfhydryl ReagentsAmino Acid SequenceMutationBase SequenceBinding SitesEscherichia coliModels, MolecularKineticsCysteineCross-Linking ReagentsStructure-Activity RelationshipRecombinant ProteinsAmino Acid SubstitutionSequence Homology, Amino AcidPlasmidsProtein Structure, TertiaryProtein BindingProtein ConformationBacterial ProteinsCloning, MolecularDNA Transposable ElementsReagent StripsCatalysisSubstrate SpecificityProtein Structure, SecondaryCell LineSequence AlignmentGenes, BacterialPoint MutationDNA PrimersMutagensAlanineEthyl MethanesulfonateHistidineReagent Kits, DiagnosticEthylnitrosoureaTransfectionMesylatesEthylmaleimideEscherichia coli ProteinsHydrogen-Ion ConcentrationConserved SequenceLysineCrystallography, X-RayCatalytic DomainSulfhydryl CompoundsDNA Mutational AnalysisDNARecombinant Fusion ProteinsSOS Response (Genetics)Protein EngineeringOligodeoxyribonucleotidesGenetic Complementation TestDithionitrobenzoic AcidRestriction MappingElectrophoresis, Polyacrylamide GelDNA, BacterialAspartic AcidCricetinaeEnzyme StabilityPolymerase Chain ReactionPhenotypeDirected Molecular EvolutionSequence DeletionAmino AcidsSaccharomyces cerevisiaeUltraviolet RaysMolecular StructureAmino Acid MotifsPhenylglyoxalPromoter Regions, GeneticMutant ProteinsGenetic VectorsTranscription, GeneticDisulfidesArginineSuccinimidesOxidation-ReductionLigandsAffinity LabelsSerineCOS CellsDNA-Binding ProteinsTemperatureAsparagine4-ChloromercuribenzenesulfonateCell MembraneMembrane ProteinsGene Expression Regulation, BacterialDiethyl PyrocarbonateCircular DichroismAntibodies, MonoclonalCHO CellsDNA-Directed DNA PolymeraseSequence Analysis, DNA
ProteinsPurificationVitroVivoMutantsMutationResiduesProteinCatalogMotifReplicatePhosphorylation sitesGeneRecombinationTranscriptionGeneratePolymeraseProductsBiologicalInhibitionQualitySubstitutionSupportCellsResultsMethodsLife ScienceToolsPerformanceCurrentTechnologyTargetPrimaryActiveAnalysisProtocolContinue
Proteins6
  • The ProQ/FinO family of RNA binding proteins mediate sRNA-directed gene regulation throughout gram-negative bacteria. (nature.com)
  • In proteins where cysteine is not within the active site, activity can be modulated by changing conformation or by influencing its regulatory role, for example, iron sulfur complexes (ISCs) in aconitase possess cysteines required for its activity [ 7 ]. (hindawi.com)
  • Allosterically regulated proteins that require an activator are sometimes based on a thiol exchange interaction involving cysteines, for example, pyruvate kinase uses fructose bisphosphate (FBPs) as a heterotrophic activator and it contains a cysteine in its FBP binding site [ 8 ]. (hindawi.com)
  • Redox proteomics has taken advantage of the thiol specificity of ICAT reagents not only to identify targets of ROS but also to quantify oxidative thiol modifications in individual proteins. (hindawi.com)
  • The first applications of this technology involved exposing purified proteins to either OS or normal condition before labeling with either heavy or light ICAT reagents, respectively. (hindawi.com)
  • Following insulin binding, the insulin receptor (IR) autophosphorylates itself and creates docking sites for IRS proteins. (scielo.br)
Purification1
  • Based on the knowledge and tools developed while identifying and characterizing new viruses and comparisons to previously-characterized viruses, new virus-specific and broad spectrum polyclonal and/or monoclonal antibody reagents, purification protocols, nucleic acid hybridization probes, PCR primers, isothermal amplification methods, and improved associated protocols will be developed. (usda.gov)
Vitro1
  • We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
Vivo2
  • Two of these methods, signature-tagged mutagenesis and in vivo expression technology, do not directly measure gene expression and do not allow true genomic-scale analysis, but they have been devised to identify genes necessary for pathogens during real infections. (cdc.gov)
  • A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)
Mutants2
  • Codon technology vastly expands the possibilities by providing thousands of site-directed mutants of each protein of interest at a fraction of the effort and cost of manual site-directed mutagenesis. (codondevices.com)
  • We used comparative protein-structure modelling to predict residues that interact with two amino acids known to be critical for hRFVT-2 function (the clinical mutants L123 and L339), site-directed mutagenesis, and truncation approach in the human-derived brain U87 cell model. (biomedcentral.com)
Mutation2
  • The second generation of our QuikChange method that provides improved fidelity over our original kit, while maintaining greater than 80% mutation efficiency for single site mutagenesis. (selectscience.net)
  • Through deletion and mutation analysis of CPT1A's promoter combined with electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) experiments, the CPT1A promoter region (-50 to -5 nt) was determined to contain two putative Sp1 binding sites, namely Sp1a and Sp1b, which might act as the GBE regulation response DNA element. (biomedcentral.com)
Residues3
  • Structure-guided mutagenesis reveals key RNA contact residues that are critical for RocC/RocR to repress the uptake of environmental DNA in L. pneumophila . (nature.com)
  • Using the reported mutant A85G/I86A as template, double-code saturation mutagenesis (DCSM) was applied at selected residues lining the substrate binding pocket with a 2-membered reduced amino acid alphabet. (springeropen.com)
  • In particular, recent studies have demonstrated that mutations at residues A85 and I86 can influence the motion of the active site loop, thereby increasing the plasticity of the binding pocket and thus raising the ability to accept non-natural substrates (Li et al. (springeropen.com)
Protein6
  • Cysteines often form part of active sites, allowing for the protein to be switched on or off depending on redox state. (hindawi.com)
  • Typically, site-directed and random mutagenesis are used to elucidate biological processes, determine protein structure, and develop screening assays, research reagents, and protein drugs. (codondevices.com)
  • Methanesulfinate (CH3SO2−) is the leaving group: RSO2S-nitroxide + protein-SH → protein-S-S-nitroxide + RSO2H The heterodisulfide bond to the cysteine residue is robust, enabling site-directed spin labelling. (wikipedia.org)
  • The cysteine can be introduced using site-directed mutagenesis, and hence most positions in a protein can be labelled. (wikipedia.org)
  • We always try to provide solutions to every protein analysis problem with the best quality reagents at the most competitive prices. (genscript.com)
  • Taxonomic classification of those E. ictaluri specific phages must rely on a synthesis of morphological and genomic details, taking into consideration that phage evolution continues to be profoundly directed by lateral gene transfer, and that a rational hierarchical technique of phage classification really should be based within the degree of DNA and protein sequence identity for several genetic loci. (hivprotease-signal.com)
Catalog1
  • No matter what methods or technology you use, Avantor offers a comprehensive catalog of the equipment, reagents, chemicals, consumables, tools, and other products you need every day. (vwr.com)
Motif1
  • Despite this inter- and intra-person heterogeneity, the mutations that most reduce antibody binding usually occur at just a few sites in the RBD's receptor binding motif. (biorxiv.org)
Replicate1
  • Bacterial pathogens must be able to gain access to, persist in, and replicate in normally privileged sites within a host. (cdc.gov)
Phosphorylation sites3
Gene2
  • The CRISPR/Cas9 is a highly effective gene editing reagent that can generate site-directed double-stranded breaks (DSB) resulting in frame-shift mutations. (usda.gov)
  • However, we now have a number of methods that allow identification of genes critical for survival in a host as well as methods that allow direct measurement of gene expression during interaction with a host. (cdc.gov)
Recombination1
  • Mutations in the analogous region of XPF also compromised XPF interactions with Rpa1 and Slx4, and are proficient in NER but deficient in ICLR and direct repeat recombination. (nih.gov)
Transcription1
  • Furthermore, NGF exposure determines a translocation of SP1 in to the nucleus where it binds to a particular region of the ncx1 promoter amongst 200 and 79 bp upstream from the transcription start out web site (15, 17). (lckinhibitor.com)
Generate1
  • alternatively, APP molecules that are subject to endocytosis are proteolyzed by BACE1 at a site further N-terminal to the membrane domain to generate a 99 aa membrane-tethered stub, termed β-CTF. (jneurosci.org)
Polymerase1
  • The enhanced efficiency of PCRBIO HiFi Polymerase minimises PCR inhibition, from impure samples such as colony PCR and direct PCR. (witec.ch)
Products2
  • We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
  • Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site. (neb.com)
Biological1
Inhibition3
  • A large number of compounds have been reported as anti-microtubule or anti-actin agents, but only a few compounds have been clarified as to their binding sites on target molecules and their inhibition mechanisms. (go.jp)
  • Furthermore, some compounds show new inhibition mechanisms by binding on novel sites in target molecules. (go.jp)
  • A novel reversible thiol-specific spin label: papain active site labeling and inhibition. (wikipedia.org)
Quality2
  • Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
  • We achieved high quality mutagenesis results and were able to complete the substitutions in our sequence. (selectscience.net)
Substitution2
  • The HiScribe T7 mRNA Kit with CleanCap Reagent AG is formatted with individual vials of NTPs and CleanCap Reagent AG to allow for partial or complete substitution of modified NTPs, with a total kit yield of 1.8 mg of mRNA. (neb.com)
  • The kinase-negative mutant kind of Akt (Akt D ) was obtained using the substitution of lysine 179 with methionine by implies of site-directed mutagenesis (Agilent Life Science, Milan, Italy) and cloned inside the pEGFP-N1-NLS expressing vector. (lckinhibitor.com)
Support3
  • In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript. (nature.com)
  • Complete kit provides all reagents necessary to support mutagenesis (at single sites), for large constructs, and for use with electroporation competent cells. (selectscience.net)
  • Find support for a specific problem in the support section of our website. (mdpi.com)
Cells1
  • Conclusions: Therefore, we identified novel phosphorylation site of IκBα by AURK, and its site was related to apoptosis and necroptosis pathway in breast cancer cells. (researchsquare.com)
Results2
Methods1
  • This a methods chapter for the alfalfa research community that guides the reader in constructing reagents for targeted mutagenesis of alfalfa genes. (usda.gov)
Life Science1
  • Epoch Life Science, Inc., a biotech company headquartered in the Nation's fastest-growing county, Fort Bend County, is a direct spin-off from the Texas Medical Center, the largest medical center in US. (epochlifescience.com)
Tools1
  • The overall approach is to develop knowledge, tools, and reagents to aid U.S. floricultural producers and diagnosticians to establish and apply effective virus testing protocols to improve clean stock production for vegetatively-propagated annuals and perennials. (usda.gov)
Performance1
  • Improvement of such traits for maximum performance typically involves mutagenesis and breeding, both of which are uncertain and require significant time. (codondevices.com)
Current2
Technology1
  • The HiScribe T7 mRNA Kit with CleanCap Reagent AG utilizes an optimized RNA synthesis formulation and trinucleotide cap analog technology for co-transcriptionally capping mRNAs that contain a natural Cap-1 structure in a single simplified reaction without compromising RNA yield. (neb.com)
Target1
  • In this chapter, we describe the construction of a CRISPR/Cas9 reagent that target the Pho2 genes. (usda.gov)
Primary1
  • Remarkably, they increase lipid uptake and M2 inflammatory macrophage infiltration in the primary tumors and metastasis to distant sites. (aacrjournals.org)
Active2
  • Such reservoir was recently named as the active HIV reservoir [ 24 ], but direct evidence behind the postulate is still lacking. (biomedcentral.com)
  • Increased flexibility of the active site loop appears to be beneficial in the directed evolution of TbSADH towards difficult-to-reduce ketones. (springeropen.com)
Analysis1
  • We propose application of iTRAQ reagents in combination with a previous thiol selection method to relatively quantify the redox state of cysteines both within and between samples in a single analysis. (hindawi.com)
Protocol1
  • IVT with CleanCap Reagent AG contained 5 mM GTP and 4 mM CleanCap Reagent AG and was performed according to recommended protocol (Standard mRNA Synthesis, HiScribe T7 mRNA Kit with CleanCap Reagent AG). (neb.com)
Continue1
  • If you continue we assume that you consent to receive all cookies on this website. (witec.ch)