• Streptavidin-HRP and chemiluminescent detection reagents are applied, and a signal is produced at each capture spot corresponding to the amount of protein bound. (rndsystems.com)
  • Add 5 × SDS Loading Buffer ( Cat# E-BC-R288 ) with the ratio of the protein sample: 5 × SDS Loading Buffer = 4:1 and boil the mixture for 10 min. (elabscience.com)
  • 1. According to the molecular weight of the target protein, prepare 0% separation gel. (elabscience.com)
  • It should be noted that studies of lymphatic vessel growth utilizing protein reagents or transgenic technology alone, in a tissue/cell culture environment, during tumor metastasis, in a lymphatic disease paradigm, or during tissue repair, have shown that various growth factors, such as platelet-derived growth factor BB (1), hepatocyte growth factor (2), fibroblast growth factor (3), and VEGF-A (4) appear to play a role. (arizona.edu)
  • It is not meant to be a discussion of protein reagents or of specific biological situations that exhibit lymphangiogenesis (see reviews 5-7). (arizona.edu)
  • Estrogen Activation of G protein-coupled Estrogen Receptor 1 Regulates Phosphoinositide 3-kinase and mTOR Signaling to Promote Liver Growth in Zebrafish and Proliferation of Human Hepatocytes. (stembook.org)
  • Whole cell extracts (30 µg lysate) of SiHa (Lane 1), K-562 (Lane 2), HEK-293 (Lane 3), MCF7 (Lane 4), MDA-MB-231 (Lane 5), HEL 92.1.7 (Lane 6), Hep G2 (Lane 7), Mouse Stomach (Lane 8), Mouse Cerebellum (Lane 9), Mouse Heart (Lane 10) were electrophoresed using NuPAGE™ 3-8% Tris-Acetate Protein Gel (Product # EA0378BOX). (thermofisher.com)
  • If using a LI-COR, additional reagents and protocol modifications are required. (rndsystems.com)
  • Weigh and smash the tissue, add an appropriate ratio of RIPA Lysis Buffer ( Cat# E-BC-R327 )(add 10 μL PMSF and 10 μL Na 3 VO 4 to each 1 mL RIPA Lysis) and homogenizely lyse the tissue. (elabscience.com)
  • For example, add 1 mL RIPA Lysis Buffer to 0.3 g tissue sample, the specific volume can be adjusted according to experimental requirements. (elabscience.com)
  • Add an appropriate ratio of RIPA Lysate Buffer (10 μL PMSF and 10 μL Na 3 VO 4 in each 1 mL RIPA Lysis) and lyse on the ice for 30 min. (elabscience.com)
  • Simple Plex Human Angiopoietin-1/ANG-1 assay kit for use on Ella instrument. (bio-techne.com)
  • We previously identified loss-of-function mutations in the angiopoietin (ANGPT) receptor TEK in families with PCG and showed that ANGPT/TEK signaling is essential for SC development. (betalifesci.com)
  • The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. (thearabidopsisbook.org)
  • Angiopoietin-like proteins stimulate HSPC development through interaction with Notch receptor signaling. (stembook.org)
  • Human IgG antibody Laboratories manufactures the tissue tek cryomold biopsy sakura reagents distributed by Genprice. (thearabidopsisbook.org)
  • The Tissue Tek Cryomold Biopsy Sakura reagent is RUO (Research Use Only) to test human serum or cell culture lab samples. (thearabidopsisbook.org)
  • Enzyme-linked immunosorbent assay (ELISA) was employed to measure levels of five serum proteins previously demonstrated to be up-regulated in papillary thyroid cancer (PTC): angiopoietin-1 (Ang-1), cytokeratin 19 (CK-19), tissue inhibitor of metalloproteinase-1 (TIMP-1), chitinase 3 like-1 (YKL-40), and galectin-3 (GAL-3). (biomedcentral.com)
  • This antibody was produced from a hybridoma (mouse myeloma fused with spleen cells from a rat immunized with purified mouse recombinant Angiopoietin-3. (reliatech.de)
  • Angiopoietin-1 (Ang-1) is a secreted glycoprotein that plays a critical role in the development and maintenance of the vascular system. (bio-techne.com)
  • Mouse Angiopoietin3 (ANG3), is a secreted glycoprotein belonging to the angiopoietin family. (reliatech.de)
  • As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. (oncotarget.com)
  • To determine whether Ang-1 and Ang-2 modulate gene expression via the Tie2/Akt pathway, we assessed the effect of Ang-1 and Ang-2 on the expression of the FOXO1 target genes Ang-2 and ESM-1 (20). (careersfromscience.org)
  • In settings where Akt activity is high, for example, in response to strong Ang-1 or Ang-2 signaling, Ang-2 expression would be shut off to prevent overstimulation of the pathway (Fig. 5that are inconsistent with Ang-2 acting as a Tie2 agonist. (careersfromscience.org)
  • The angiopoietin (ANGPT)-TIE2/TEK signaling pathway is essential for blood and lymphatic vascular homeostasis. (betalifesci.com)
  • Human Angiogenesis Panel 1 Detection Antibodies (Cat. (biolegend.com)
  • Angiopoietin-1 can protect against pulmonary arterial hypertension, reduce the extent of fibrosis and remodeling in infarcted diabetic myocardium, and enhance tumor progression and metastasis. (bio-techne.com)
  • GTPCH interacted with Ang-1 in stromal fibroblasts and enhanced Ang-1 expression and function, which in turn phosphorylated tumor Tie2 and induced cell proliferation. (oncotarget.com)
  • In coimplantation xenografts, GTPCH in fibroblasts enhanced tumor growth, upregulating Ang-1 and alpha-smooth muscle actin mainly in fibroblast-like cells. (oncotarget.com)
  • GTPCH/Ang-1 interaction in stromal fibroblasts and activation of Tie2 on breast tumor cells could play an important role in supporting breast cancer growth. (oncotarget.com)
  • The MEC13.3 antibody specifically recognizes CD31, also known as PECAM-1 (Platelet Endothelial Cell Adhesion Molecule-1). (bdbiosciences.com)
  • Angiopoietin-1 induces the development and branching of blood vessels, promotes vascular endothelial cell motility during angiogenesis, and enhances vascular integrity. (bio-techne.com)
  • The development of carcinoma requires a complex surrounding microenvironment, which is composed of extracellular matrix (ECM), soluble growth factors, stromal cells and blood vessels [ 1 ]. (oncotarget.com)
  • Nevertheless, real-time PCR evaluation exposed that Ang-1 and Ang-4 are indicated at suprisingly low or undetectable amounts in HUVECs and so are not really induced by FOXO1 (data not really shown). (careersfromscience.org)
  • ECRs visited Dundee for 1-11 months each and performed collaborative research which is leading to longer-term future interactions. (ukri.org)
  • From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the "Elabscience Publication Reward Application Form" carefully and send it to [email protected] , we will get back to you with the reward after we confirm it ASAP! (elabscience.com)
  • Western blot analysis of extracts of various cell lines using Angiopoietin 2 Polyclonal Antibody at dilution of 1:3000. (elabscience.com)
  • Immunofluorescence analysis of HeLa cells using Angiopoietin 2 Polyclonal Antibody at dilution of 1:100 (40x lens).Blue: DAPI for nuclear staining. (elabscience.com)
  • To test whether Tie2/Akt signaling induced by autocrine Ang-2 has an inhibitory effect on FOXO1 transcriptional activity similar to the effect of Ang-1, we examined the effect of Ang-2-blocking antibody on the expression of the FOXO1 target genes Ang-2 and decorin, genes that we have shown to be induced by FOXO1 and repressed by Ang-1 (20). (careersfromscience.org)
  • As shown in Fig. 5data confirms that Ang-2, like Ang-1, can activate Tie2/Akt signaling, inhibit the expression of FOXO1 target genes, and inhibit vascular leak. (careersfromscience.org)
  • The Ang-1- and Ang-2-mediated changes in expression of both target genes were statistically significant at 0.01. (careersfromscience.org)
  • Based on a review of these and other published studies using gene expression analysis, we concluded that YKL-40, Gal-3, cytokeratin 19 (CK19), TIMP-1 and Ang-1 were consistently found to be upregulated. (biomedcentral.com)
  • Calcium-inducible MAPK/AP-1 signaling drives semaphorin 3A expression in regular human epidermal keratinocytes. (primary-antibodiescom.com)
  • If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received. (elabscience.com)
  • Store all reagents at 2-8 degree C. (doronscientific.com)
  • Since applications vary, each investigator should titrate the reagent to obtain optimal results. (bdbiosciences.com)
  • Incidence has increased by 6.8% per year in males and 8.8% per year in females since 1998 in Canada with similar results noted in Europe and the United States [ 1 ]. (biomedcentral.com)