SpecificityImmunogenCross reactivityDatasheetDetects endogenous levelsPurificationEndogenous levelsAntigenSecondary AntibodyHumanConjugateProteinsResiduesPhosphopeptideCloneMonoclonal antibodiesCatalogDilutionPhosphorylationCustom antibody servicesGlycerolNeurosciencePurified by affinity-chromatographyPolyclonal AntibodiesAlexa FluorMembranePrimaryMouseLanesApoptosisSequenceSmall MoleculesWestern BlotEpitopesConcentrationSer473ProductPhosphorylated at serineGlycoproteins
Specificity4
- Specificity analysis using Rabbit Anti-ALK (pTyr1507) Polyclonal Antibody (SPC-913). (2bscientific.com)
- At CST, we adhere to the Hallmarks of Antibody Validation™ , six complementary strategies for determining the specificity, sensitivity, and functionality of an antibody in any given assay. (cellsignal.jp)
- Specificity This antibody detects endogenous human Bcl-2 only when phosphorylated at serine 70.Accession no. (qedbio.com)
- Production and use of mAbs, generated in biofactories, represent vast potential as aids in immunological responses when the organism cannot produce them on their own, these convey unique specificity by recognizing and targeting specific antigen. (bvsalud.org)
Immunogen2
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. (origene.com)
- Affinity-purified using epitope specific immunogen. (biovisi.com)
Cross reactivity4
- No cross reactivity with nonacetylated lysine, and lysine with other modification. (sigmaaldrich.com)
- A BLAST analysis was used to suggest cross-reactivity with STAT2 pS734 protein from human and monkey sources based on homology with the immunizing sequence. (rockland.com)
- Due to limited amount of testing and knowledge, not every possible cross-reactivity is known. (ningbobio.com)
- This report describes generation of a recombinant Sudan virus and demonstrates its utility by quantifying antibody cross-reactivity between Ebola and Sudan virus glycoproteins after human infection or vaccination with a licensed Ebola virus vaccine. (cdc.gov)
Datasheet2
- If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase. (thermofisher.com)
- The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. (ptglab.com)
Detects endogenous levels3
- Phospho-Stat5 (Tyr694) Rabbit Monoclonal Antibody detects endogenous levels of Stat5a only when phosphorylated at Tyr694 and Stat5b when phosphorylated at Tyr699. (ebiocell.com)
- This antibody detects endogenous levels of PI3 Kinase p85/p55 protein only when phosphorylated at Tyr467/199. (arigobio.com)
- Phospho-GFAP (Ser38) Antibody detects endogenous levels of GFAP only when phosphorylated at Serine 38. (affbiotech.com)
Purification3
- This antibody is purified through a protein A column, followed by peptide affinity purification. (avivasysbio.com)
- Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns. (aveslabs.com)
- The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns. (affbiotech.com)
Endogenous levels1
- Specific for endogenous levels of the ~135 kDa KCC2 protein phosphorylated at Ser940. (aveslabs.com)
Antigen4
- This Antibody was verified by Independent antibody to ensure that the antibody binds to the antigen stated. (thermofisher.com)
- p70 S6K (phospho Thr421/Ser424) antibody [HL5] detects p70 S6K (phospho Thr421/Ser424) protein at cytoplasm by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right). (genetex.com)
- Western blot analysis of ATF2 phosphorylation expression in MDA-MB-435 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. (zfdows.com)
- In the process of engaging the antigen, antibodies complementarity-determining regions (CDR) may need to navigate interactions with or concealment by lipid molecules. (elifesciences.org)
Secondary Antibody2
- Secondary Antibody: Anti-Rabbit IgG: HRP at 1:100000.WT Peptide Sequence: N-P-T-pY-G-S-W. SPOT peptide synthesis was used to perform substitution analysis of the phospho-acceptor AA in the sequence that was used to produce the phospho-site-specific antibody. (2bscientific.com)
- Flow cytometric analysis of Jurkat cells secondary antibody only negative control (blue) untreated (red) treated with IFNα IL-4 and pervanadate (green) treated + blocked with phospho-peptide (brown) using Phospho-Stat3 (Tyr705) antibody Stat3Y705-B12 (5 ng/mL) Cat. (abwizbio.com)
Human19
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing staining of proteins containing phosphorylated 14-3-3 binding motifs, using Phospho-(Ser) 14-3-3 Binding Motif Antibody. (cellsignal.com)
- MA5-14485 targets Epidermal Growth Factor Receptor in IHC (P) applications and shows reactivity with Human samples. (thermofisher.com)
- Phospho-RhoA (Ser-188) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 188 of human RhoA. (phosphosolutions.com)
- Phospho-Crk (Tyr-221) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Tyr-221 in human Crk II. (phosphosolutions.com)
- The antibody detects a 40 kDa* protein corresponding to the molecular mass of Crk II on SDS-PAGE immunoblots of human K562 cells stimulated with pervanadate. (phosphosolutions.com)
- Phospho-VASP (Thr-278) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding threonine 278 in human VASP. (ecmbio.com)
- This MEF2C Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S387 of human MEF2C. (avivasysbio.com)
- Carrier-protein conjugated synthetic peptide surrounding phospho Thr421/Ser424 of human p70 S6K. (genetex.com)
- AFX (Phospho-Ser197) antibody was raised against a peptide sequence around phosphorylation site of serine 197 (A-A-S (p) -M-D) derived from Human AFX. (prosci-inc.com)
- Synthetic peptide corresponding to Human Histone H3 (phospho S10). (abcam.com)
- No. 425401) then stained with anti-human CD20 APC and anti-STAT5 Phospho (Tyr694) (clone A17016B.Rec) PE/Cyanine7. (biolegend.com)
- Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and nuclear localization, using Phospho-Akt (Ser473) (736E11) Rabbit mAb. (cellsignal.jp)
- Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Phospho-Akt (Ser473) (736E11) Rabbit mAb preincubated with an irrelevant peptide (left) or Phospho-Akt (Ser473) Blocking Peptide (#1140) (right). (cellsignal.jp)
- STAT2 pS734 antibody was prepared from whole rabbit serum produced by repeated immunizations with a phosphorylated synthetic peptide corresponding to serine 734 a region near the C-terminus of human STAT2 protein. (rockland.com)
- STAT2 pS734 affinity purified antibody is directed against human STAT2 protein. (rockland.com)
- This antibody is specific for phosphorylated S734 human STAT2. (rockland.com)
- Additionally, there are different types of mAbs (murine, chimeric, humanized, human, mAbs as Antibody-drug conjugates and bispecific mAbs) discussed in the present work. (bvsalud.org)
- 28740-1-AP targets Phospho-EIF2S1 (Ser51) in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples. (ptglab.com)
- Phosphospecific peptide around Tyr467/199 of Human PI3 Kinase p85/p55. (arigobio.com)
Conjugate1
- Immunoprecipitation of NIH/3T3 cell lysates, untreated or PDGF-treated, using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb (Sepharose ® Bead Conjugate) compared to supernatants collected after immunoprecipitation from the same lysate. (cellsignal.jp)
Proteins7
- Western blot analysis of extracts from 293 cells transfected with Bad or Bad Ser112 and/or Ser136 mutant fusion proteins, using Phospho-(Ser) 14-3-3 Binding Motif Antibody (top), Phospho-Bad (Ser136) Antibody #9295 (middle) or Phospho-Bad (Ser112) Antibody #9291 (bottom). (cellsignal.com)
- Abcam: antibodies, proteins, kits. (abcam.com)
- This antibody reacts to lysine-acetylated proteins. (sigmaaldrich.com)
- Antibodies can be defined as heterotetrametric glycoproteins of symmetric nature, and they participate as effector proteins in humoral responses. (bvsalud.org)
- The modeling platform developed here uncovers insights into lipid participation in antibodies' recognition of membrane proteins and highlights antibody features to prioritize in vaccine design. (elifesciences.org)
- Antibodies can target epitopes on integral membrane proteins very near to the lipid bilayer surface, even those partially embedded within the headgroup region. (elifesciences.org)
- Chemical synthesis of proteins is a valuable tool in chemical biology as it allows for the introduction of non-natural amino acids as well as residue specific incorporation of "posttranslational modifications" such as phosphorylation, glycosylation, acetylation, and even ubiquitination. (wikipedia.org)
Residues2
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser940 of rat KCC2, conjugated to keyhole limpet hemocyanin (KLH). (aveslabs.com)
- Engineered mutations to the 10E8 paratope at light-chain solvent-exposed residues that add positive charge or hydrophobic sidechains proved to increase antibody association to anionic phospholipid vesicles in vitro and also boosted neutralization potency 27 . (elifesciences.org)
Phosphopeptide4
- Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. (prosci-inc.com)
- Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. (prosci-inc.com)
- The antibody was purified using epitope specific phosphopeptide. (abcam.com)
- The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. (abcam.com)
Clone2
- This recombinant antibody clone is derived from clone A17016B. (biolegend.com)
- While polyclonal antibodies are secreted by many different B cell clones and recognize multiple antigenic epitopes, monoclonals originate from a single B cell clone and are specific for just one epitope. (cellsignal.jp)
Monoclonal antibodies6
- Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. (abcam.com)
- Traditional polyclonal and monoclonal antibodies are the product of normal B cell development and genetic recombination. (cellsignal.jp)
- In contrast, hybridoma-based systems for producing monoclonal antibodies are subject to genetic drift and instability, increasing the potential for lot-to-lot variability or loss of antibody expression. (cellsignal.jp)
- ELISA, immunofluorescence, and Western blot assay results revealed the recognition of Myo1g by these novel monoclonal antibodies in normal and transformed T and B cells. (bvsalud.org)
- Here, we report the development and application of new monoclonal antibodies against Myo1g for their potential use to detect its overexpression in acute lymphoblastic leukemia (ALL) patients. (bvsalud.org)
- After booster immunization, mouse spleen lymphocytes were fused with mouse myeloma cells, and 24 hybridoma cell lines of the monoclonal antibodies against puerarin were screened by monoclonal antibody screening technique. (bvsalud.org)
Catalog2
- The following product was used in this experiment: Phospho-EGFR (Tyr1068) Monoclonal Antibody (EP38Y) from Thermo Fisher Scientific, catalog # MA5-14485, RRID AB_10977041. (thermofisher.com)
- The following product was used in this experiment: Phospho-CaMKII alpha/beta/delta (Tyr231) Polyclonal Antibody from Thermo Fisher Scientific, catalog # PA5-17108, RRID AB_10987028. (thermofisher.com)
Dilution3
- Please refer to primary antibody product webpage for recommended antibody dilution. (cellsignal.com)
- Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 28740-1-AP (Phospho-EIF2S1 (Ser51) antibody) at dilution of 1:2000 incubated 4℃ overnight. (ptglab.com)
- Western blot: COS7 cells treated with of without H2O2 stained with ARG66216 anti-PI3 Kinase p85 / p55 phospho (Tyr467 / 199) antibody at 1:1000 dilution. (arigobio.com)
Phosphorylation3
- Immunocytochemical labeling of VASP phosphorylation in rabbit spleen fibroblasts treated with Calyculin A. The cells were labeled with rabbit polyclonal VASP (Thr-278) antibody, then detected using appropriate secondary antibodies conjugated to Cy3. (ecmbio.com)
- Changes in phosphorylation levels of Ser-98 and Ser-179 in Ldb3 were further confirmed in NRVMs and other pathological/physiological hypertrophy models, including transverse aortic constriction and swimming models, using site-specific phospho-antibodies. (molcells.org)
- However, there was immunohistochemical reactivity for phosphorylated AMP-dependent kinase (p-AMPK Thr172) and glycogen synthase kinase 3 (GSK3) phosphorylation (p-GSK3 Ser12), and nuclear expression of cyclin D1. (dundee.ac.uk)
Custom antibody services1
- Founded in 2001, we are committed to offering the highest quality antibodies, custom antibody services, and customer support in the business. (phosphosolutions.com)
Glycerol3
- Rabbit polyclonal, affinity-purified antibody is supplied in 100μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. (ecmbio.com)
- Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. (prosci-inc.com)
- Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. (ningbobio.com)
Neuroscience1
- PhosphoSolutions is your source for neuroscience and cancer Antibodies that Work™ made and tested right here in Denver, Colorado. (phosphosolutions.com)
Purified by affinity-chromatography1
- The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions. (biolegend.com)
Polyclonal Antibodies1
- Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.jp)
Alexa Fluor1
- Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. (ningbobio.com)
Membrane10
- For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween ® 20 at 4°C with gentle shaking, overnight. (cellsignal.com)
- OAAB16210) and anti-MEF2C Non Phospho-specific Pab on nitrocellulose membrane. (avivasysbio.com)
- Wild-type (WT) and p70 S6K (phospho Thr421/Ser424) knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with p70 S6K (phospho Thr421/Ser424) antibody [HL129] (GTX635621) diluted at 1:500. (genetex.com)
- The membrane was stripped and re-blotted with GAPDH antibody as loading control. (ptglab.com)
- A potent class of HIV-1 broadly neutralizing antibodies (bnAbs) targets the envelope glycoprotein's membrane proximal exposed region (MPER) through a proposed mechanism where hypervariable loops embed into lipid bilayers and engage headgroup moieties alongside the epitope. (elifesciences.org)
- All-atom simulations of 4E10, PGZL1, 10E8 and LN01 docked onto HIV-like membranes consistently form phospholipid complexes at key complementarity-determining region loop sites, solidifying that stable and specific lipid interactions anchor bnAbs to membrane surfaces. (elifesciences.org)
- This valuable study reports multi-scale molecular dynamics simulations to investigate a class of highly potent antibodies that simultaneously engage with the HIV-1 Envelope trimer and the viral membrane. (elifesciences.org)
- The work provides insights into how broadly neutralizing antibodies associate with lipids proximal to membrane-associated epitopes to drive neutralization. (elifesciences.org)
- A better understanding of how antibodies develop membrane affinity and target membrane-proximal epitopes would be impactful for antibody therapeutics, auto-immunity, and vaccine development 10 - 12 . (elifesciences.org)
- We sought to address this phenomenon for broadly neutralizing antibodies (bnAbs) 4E10, PGZL1, 10E8, and LN01 of unique lineages which all target the semi-concealed membrane-proximal epitope region (MPER) 13 - 16 of the HIV-1 envelope glycoprotein (Env). (elifesciences.org)
Primary3
- Primary Antibody: Rabbit Anti-ALK (pTyr1507) Polyclonal Antibody (SPC-913) at 1:1000. (2bscientific.com)
- The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (genetex.com)
- Our Anti-Potassium Chloride Cotransporter (KCC2) (Ser940) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. (aveslabs.com)
Mouse1
- The blot was treated with alkaline phosphatase to dephosphorylate Crk II (lanes 2 & 4), then the blot was probed with mouse monoclonal Crk II (C-terminal region) CM3321 (lanes 1 & 2) and Crk II (Tyr-221) phospho-specific CP4701 (lanes 3 & 4). (phosphosolutions.com)
Lanes2
- The latter antibody was used in the presence of no peptide (lanes 3 & 4), phospho-Rho (Ser-188) peptide (lane 5), or a non-specific phosphoserine peptide (lane 6). (phosphosolutions.com)
- antibody (lanes 1 & 2) or rabbit polyclonal VASP (Thr-278) phospho-specific antibody (lanes 3 & 4). (ecmbio.com)
Apoptosis1
- Akt, also known as Protein kinase B (PKB), is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration. (arigobio.cn)
Sequence2
- No changes or modifications were done to the antibody sequence or scaffold. (biolegend.com)
- From the 142 confirmed cases, we performed unbiased (Illumina) and SUDV-amplicon-specific (Minion) high-throughput sequencing to obtain 120 SUDV genome-and coding-complete sequences, representing 95.4% (104/109) of SVD-confirmed individuals within a sequence-able range (Ct 30) and 10 genome sequences outside of this range and 6 duplicate genome sequences. (cdc.gov)
Small Molecules1
- In contrast to research using biochemistry, genetics, or molecular biology, where mutagenesis can provide a new version of the organism, cell, or biomolecule of interest, chemical biology probes systems in vitro and in vivo with small molecules that have been designed for a specific purpose or identified on the basis of biochemical or cell-based screening (see chemical genetics). (wikipedia.org)
Western Blot2
- Western blot analysis of extracts from Jurkat cells, untreated or calyculin A-treated (0.1 µM for 30 minutes), using Phospho-(Ser) 14-3-3 Binding Motif Antibody. (cellsignal.com)
- The western blot was probed using Phospho-Akt Substrate (RXXS*/T*) (110B7E) Rabbit mAb #9614. (cellsignal.jp)
Epitopes2
- We used immunohistochemistry and antibodies against phospho-specific epitopes to examine the activity of three potential signalling pathways in tumour cells of three genetically confirmed cases of SDHB-associated renal cell carcinomas. (dundee.ac.uk)
- The aim of this study was to investigate the roles of different signalling pathways in the pathogenesis of these tumours.Methods and resultsWe used immunohistochemistry and antibodies against phospho-specific epitopes to examine the activity of three potential signalling pathways in tumour cells of three genetically confirmed cases of SDHB-associated renal cell carcinomas. (dundee.ac.uk)
Concentration2
- 1 mg/ml (Please refer to the vial label for the specific concentration. (genetex.com)
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool. (biolegend.com)
Ser4732
- Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using Phospho-Akt (Ser473) (736E11) Rabbit mAb (left) or PTEN (138G6) Rabbit mAb #9559 (right). (cellsignal.jp)
- Immunohistochemical analysis using Phospho-Akt (Ser473) (736E11) Rabbit mAb on SignalSlide (R) Phospho-Akt (Ser473) IHC Controls #8101 (paraffin-embedded LNCaP cells, untreated (left) or LY294002-treated (right). (cellsignal.jp)
Product1
- By carefully tailoring these strategies to each antibody product, we guarantee that CST antibodies will work as expected, to help you achieve results you can trust. (cellsignal.jp)
Phosphorylated at serine1
- This antibody detects endogenous level of AFX only when phosphorylated at serine 197. (prosci-inc.com)
Glycoproteins1
- Methods have been developed that include the use of antibodies, lectins to capture glycoproteins, and immobilized metal ions to capture phosphorylated peptides and enzyme substrates to capture select enzymes. (wikipedia.org)