Clear supernatantPelletDiscardCentrifugePolymeraseAssayCentrifugationSpectrophotometricallyAntibodiesRoom temperatureDiluteAliquotTubesChromatinStoolAntibodyBufferCarefullyAqueousAmmoniumEnzymeProteinReagentCellsNucleophilicLyseConcentrationPrepareRemoveYieldMethodsPotassiumAdjustSampleStopGentlyReverseMaterialWaterImportantMethodColdActivityEasyAdditionConditionsCellPresenceStoreQuickPatientsPlatesAnalysis
Clear supernatant1
- If turbidity is observed, centrifuge sample and use clear supernatant for assay. (gentaur.nl)
Pellet7
- Dissolve the pellet by adding an appropriate amount of reaction buffer. (sigmaaldrich.com)
- Assay the supernatant, and the solution from the pellet, for protein content and/or enzyme activity. (sigmaaldrich.com)
- Air dry the pellet and resuspend the mRNA in 50 μl of 0.1 mM EDTA or a suitable RNA storage solution. (neb.com)
- Discard supernatant and resuspend cell pellet in 1 mL of PBS. (mdanderson.org)
- g Pour off the liquid (supernatant) into a boiling tube being careful not to lose the pellet. (practicalbiology.org)
- Aspirate supernatant without disturbing pellet. (biolegend.com)
- C and centrifuged at 12 500 30 The supernatant was vacuum-dried and the pellet was resuspended in 200?μL water-saturated butanol. (bioerc-iend.org)
Discard4
- Aspirate the supernatant and discard. (mdanderson.org)
- Carefully remove and discard the supernatant, resuspend and repeat 2 times. (neb.com)
- To wash, add immobilization buffer to the resin and agitate for one minute, centrifuge (1 minute at 11,000 x g at 4°C), carefully remove and discard the supernatant, and repeat. (neb.com)
- 6. Spin the cells (350 x g) and discard the supernatant. (biolegend.com)
Centrifuge1
- She puts it in a centrifuge that spins the sealed buckets to separate the solid material from the liquid supernatant. (cdc.gov)
Polymerase6
- The mRNA expression of MMP-2 and MMP-9 was determined by reverse transcription-polymerase chain reaction, and the protein expression of MMP-2 and MMP-9 by gelatin zymography analysis and qualified by the gel electrophoresis image analysis in different HA concentrations. (molvis.org)
- We investigated the effects of different level of HA concentrations on mRNA and protein expressions of MMP-2 and MMP-9 in cultured trabecular meshwork cells using reverse transcription-polymerase chain reaction (RT-PCR) method and gelatin zymography analysis. (molvis.org)
- Determination Tanespimycin price of the patients' IL28B genotype (SNP rs12979860) was performed on human genomic DNA by a real-time polymerase chain reaction on the ABI 7900HT platform. (cftrpathway.com)
- ABSTRACT This study aimed to establish an accurate and sensitive polymerase chain reaction (PCR) technique for the diagnosis of active human brucellosis in Egypt. (who.int)
- A CDC scientist adds chemical reagents to tubes to prepare them for molecular testing using polymerase chain reaction (PCR). (cdc.gov)
- more recently polymerase chain reaction, PCR (Medeiros et al. (bvsalud.org)
Assay4
- BioAssay Systems' EnzyChromTM Creatine Kinase Assay Kit is based on enzyme coupled reactions in which creatine phosphate and ADP is converted to creatine and ATP by CK, the generated ATP is used to phosphorylate glucose by hexokinase to generate glucose-6-phosphate, which is then oxidized by NADP in the presence of glucose-6-phosphate dehydrogenase. (gentaur.nl)
- For each reaction well, mix 10 µL Substrate Solution, 100 µL Assay Buffer and 1 µL Enzyme Mix. (gentaur.nl)
- This assay is based on an enzyme-catalyzed kinetic reaction. (gentaur.nl)
- Endopep-MS currently uses an antibody-affinity concentration/purification step before the enzymatic reaction with the substrate, and the choice of antibody is critical for the success of this assay [ 5 ]. (cdc.gov)
Centrifugation1
- After cytolysis the TDA is released and is found to be in the supernatant after centrifugation. (bmglabtech.com)
Spectrophotometrically2
- The absorbance of the supernatant was measured spectrophotometrically at 280 nm. (cftrpathway.com)
- The cyanide is measured spectrophotometrically after a colorimetric reaction involving the cyanide ion and chloramines-T plus pyridine-pyrazolone, p -benzoquinone, or p -phenylene diamine (see Table 7-1). (cdc.gov)
Antibodies2
- Where polyclonal antibodies are purified directly from the serum of the immunized host, and monoclonals are purified from either hybridoma-derived tissue culture supernatant or ascites, recombinant antibodies are instead purified from the tissue culture supernatants of transfected host cell lines. (cellsignal.com)
- During the first incubation, adenovirus antigens present in the stool supernatant are captured by antibodies attached to the wells. (rapidtest.com)
Room temperature5
- Sucrose 34.23 g (0.4 M) KCl 0.19 g (0.01 M). Dissolve in phosphate buffer solution (pH 7.0) at room temperature and make up to 250 cm 3 with the buffer solution. (practicalbiology.org)
- 3 Potassium chloride 0.05 M. Dissolve 0.93 g in phosphate buffer solution at room temperature and make up to 250 cm 3 . (practicalbiology.org)
- Warm the 1X solution to room temperature prior to use. (biolegend.com)
- Incubate at room temperature or 37°C. CK is fully activated within 20 min by glutathione provided in the Substrate Solution. (gentaur.nl)
- The solution was gently inverted ten times, and kept for 45 minutes at room temperature to precipitate erythrocytes. (bvsalud.org)
Dilute1
- If you plan to do both qPCR and microarray hybridization then first dilute DNA pellets in 12ml of pure H2O transfer 4ml of DNA solution to a new eppendorf tube and add 46ml of pure H2O. (chip-atlas.org)
Aliquot1
- Add 5ml of 100mM PMSF solution in isopropanol to each 500ml aliquot of precleared chromatin. (chip-atlas.org)
Tubes4
- Immediately fill tubes up to 50 mL with cold 1X lysing solution. (mdanderson.org)
- Decant supernatant and allow tubes to drain briefly. (mdanderson.org)
- Transfer supernatants to new tubes. (chip-atlas.org)
- Microfilariae enriched supernatant was washed with a saline solution twice and the sediment was transferred to Eppendorff® tubes and fixed with 1 mL of a mixture of glacial acetic acid (Sigma Chem. (bvsalud.org)
Chromatin1
- Lysates were centrifuged at full speed for 5 minutes at 4°C, and the supernatant containing the sonicated chromatin was transferred to a new tube. (nih.gov)
Stool2
- It is a double antibody (sandwich) ELISA using a polyclonal anti-adenovirus antibody to capture the antigen from the stool supernatant. (rapidtest.com)
- To mix the stool and the chemicals, she puts the stool in with the cell culture media and glass beads, suspending the stool to get it into solution. (cdc.gov)
Antibody2
- Blocked antibody-conjugated beads were then placed on a magnet, supernatant was removed, and the sonicated lysate was added to the beads followed by incubation for 3-4 hours at 4°C on a rotator. (nih.gov)
- 3. Add appropriate amount of antibody to each reaction. (chip-atlas.org)
Buffer9
- Add that portion directly to the reaction buffer. (sigmaaldrich.com)
- 0.05 M phosphate buffer solution, pH 7.0: Store in a refrigerator at 0-4 °C ( Note 1 ). (practicalbiology.org)
- 1 0.05 M phosphate buffer solution, pH 7.0. (practicalbiology.org)
- 4 DCPIP solution DCPIP 0.007-0.01 g, made up to 100 cm 3 with phosphate buffer. (practicalbiology.org)
- Prepare a protein solution containing up to 1 mg/ml SNAP-tag fusion protein to be immobilized in an appropriate buffer containing at least 1 mM DTT. (neb.com)
- RBC Lysis Buffer is supplied as a 10X solution containing ammonium chloride, potassium carbonate, and EDTA, and should be diluted in deionized water prior to use. (biolegend.com)
- This red blood cell (RBC) lysis buffer is supplied as a 10X solution and should be diluted to 1X in deionized water. (biolegend.com)
- 5. Stop the reaction by diluting the Lysis Buffer with 20-30 ml of 1X PBS. (biolegend.com)
- Incubated with freshly made 1.8% formaldehyde in X-linking buffer in the presence of of n-heptane.After incubation, embryos were re-suspended in Stop solution Washed at +40C in ChIP wash A solution and then at +40C in ChIP wash B. After sedimentation of the embryos ChIP wash B solution was removed and the embryos were used in an experiment or frozen in liquid N2. (chip-atlas.org)
Carefully1
- Remove the supernatant carefully. (neb.com)
Aqueous3
- Their industrial-scale preparation has been designed and uses sodium hydroxide as the base in a very concentrated aqueous CD solution [3] . (beilstein-journals.org)
- The remaining aqueous solution was made acidic to pH 2.0 with HCl 6 N and then extracted with ethyl acetate (5x150 mL). (scialert.net)
- Combine aqueous phases (you will get 120ml + 150ml=270ml of solution). (chip-atlas.org)
Ammonium3
- It is not necessary to remove absolutely all of the ammonium sulfate solution. (sigmaaldrich.com)
- It is likely that only negligible amounts of enzyme will be in the ammonium sulfate solution. (sigmaaldrich.com)
- 55 It was found that the solventfree reaction of ETFAA 1a, benzaldehyde, and ammonium acetate at 100 C proceeded with the loss of the water molecule to aff ord partially dehydrated 2-hydroxy-2,6-bis(trifl uoromethyl)-1,2,3,4-tetrahydropyridine-3,5-dicarboxylate 6. (researchgate.net)
Enzyme3
- The PPI dendrimer encapsulated Cu ions in the internal nanovoids to form adjacent Cu species, which exhibited significantly high catalytic activity for the regioselective coupling reaction of DMP compared to previously reported enzyme and metal complex catalysts. (mdpi.com)
- We also offer solutions for automation, site-directed mutagenesis, as well as your favorite restriction enzyme, ligase or competent cell products. (neb.com)
- Keep solutions and apparatus cold during the extraction procedure, steps 1-8, to preserve enzyme activity. (practicalbiology.org)
Protein3
- Add this protein solution to the SNAP-Capture Pull Down Resin in a 1.5 ml microcentrifuge tube (20 µl bed-volume resin) prepared as above. (neb.com)
- The washing step removes non-specifically bound protein after the immobilization reaction. (neb.com)
- This assembly results in a spontaneous, essentially traceless splicing reaction that covalently links the two flanking protein segments through native chemical ligation 9 . (biorxiv.org)
Reagent1
- Briefly, 1.0 mL Folin-Ciocalteu s reagent (50%) and 0.8 mL 7.5% (w/v) Na 2 CO 3 were added to 0.2 mL (500 ppm) of methanolic solution of EtOAc fraction. (scialert.net)
Cells3
- Cells were collected at 500 x g for 10 minutes at 4°C (subsequent work was performed on ice and used cool buffers and solutions unless otherwise specified) and washed twice with up to 0.5 ml ice-cold PBS supplemented with 1 μM PMSF. (nih.gov)
- When local integration of the cells occurred, they were washed twice again with Hank's solution. (molvis.org)
- Washed red blood cells are incubated in an isotonic sucrose solution containing normal ABO compatible serum. (medscape.com)
Nucleophilic2
- We synthesise these materials using the efficient nucleophilic thiol-yne reaction between a dipropiolamide and dithiol to yield an α,β − unsaturated carbonyl moiety along the polymer backbone. (nature.com)
- The review is summarized and systematized the published data on the multicomponent reactions of ethyl trifluoroacetoacetate, carbonyl compounds (aldehydes and ketones), and nucleophilic reagents. (researchgate.net)
Lyse1
- 100 ml of 10X concentrate will yield a quantity of 1X solution that is sufficient to lyse 500 samples. (biolegend.com)
Concentration2
- Use the stock with concentration of 0.5% of input DNA per ml of solution (see: ?Isolation of ChIP Input DNA? (chip-atlas.org)
- In order to overcome this, the biofilm growth in SSFs is modelled using a system of non-linear balance laws of convection-diffusion-reaction type with discontinuous flux for the concentration of species in the biofilm matrix and the flowing liquid suspension. (lu.se)
Prepare1
- From supernatant, prepare washed cell 50% solution. (medscape.com)
Remove1
- The extracts were collected, filtered and the volume was reduced under vacuum at 40°C. After the pH of the solution adjusted to 11.0 by NaOH 4 N, it was extracted with chloroform (3x150 mL) to remove basic material that mainly contains alkaloids. (scialert.net)
Yield1
- The present approach shows the significant outcomes such as yield up to 94%, catalyst‐free reaction, easy workup procedure and shorter period of reaction time. (researchgate.net)
Methods1
- The family of HDG methods is a type of finite element methods that use discontinuous basis functions constructed from local finite element spaces on each element, introducing an approximation of the trace of the solution on each inter-element boundary, and the prescription of a numerical flux across these. (lu.se)
Potassium1
- Note that Potassium chloride is a cofactor for the Hill reaction. (practicalbiology.org)
Sample1
Stop2
- The stop solution ends the reaction and turns the blue color to yellow. (rapidtest.com)
- Glycine was added to stop the reaction. (nih.gov)
Gently1
- 3. Gently vortex each tube immediately after adding the lysing solution. (biolegend.com)
Reverse1
- CK catalyzes the conversion of creatine to phosphocreatine, consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP) and the reverse reaction. (gentaur.nl)
Material1
- By rationally exploiting reaction conditions, the alkene stereochemistry is modulated between 35-82% cis content and the stereochemistry dictates the bulk material properties such as tensile strength, modulus, and glass transition. (nature.com)
Water2
Important2
- One of the most important reactions of CDs occurs with oxiranes (epoxides) under basic conditions to provide (2-hydroxy)propyl-CDs (HPCDs) that are commonly used in pharmaceutical formulations and household products. (beilstein-journals.org)
- UDP-glucuronosyltransferase (UGT)-mediated metabolism is possibly the most important conjugation reaction for marketed drugs. (aspetjournals.org)
Method1
- The main aim of the first project is partly to obtain a comprehensive mathematical-ecological model formulated as a system of PDEs with a spatially discontinuous velocity, and partly to create a numerical method to approximate the solution. (lu.se)
Cold1
- Embryos not older than 16h were collected, bleached in 2.5-3.0% Na-hypochlorite solution, washed two times in cold EWB. (chip-atlas.org)
Activity1
- As expected, addition of starch induced the expression of amyE encoding amylase and increased amylase activity in fermentation solution. (hindawi.com)
Easy1
- It is not quite so easy to demonstrate the other reactions in photosynthesis. (practicalbiology.org)
Addition1
- The control experiment was carried out using the casein solution without the addition of serine proteinases. (cftrpathway.com)
Conditions1
- Under the optimal conditions of pH 0 of 3, current density of 5 mA/cm 2 , Fe 0 dosage of 3 g/L, and H 2 O 2 dosage of 0.059 M, the electro-Fe 0 /H 2 O 2 reaction showed the removal efficiencies of COD and chromaticity for coagulated effluent were 76.68% and 74%, respectively. (mdpi.com)
Cell1
- In the cell, NADP is the electron acceptor which is reduced in the light-dependent reactions, and which provides electrons and hydrogen for the light-independent reactions. (practicalbiology.org)
Presence1
- In the presence of a solution containing Eu 3+ , the released TDA forms a highly fluorescent and stable chelate (EuTDA), whose levels are measured. (bmglabtech.com)
Store1
- Store DNA solutions at -200C. (chip-atlas.org)
Quick1
- The kit includes LiCl solution for quick recovery of the synthesized mRNA. (neb.com)
Patients1
- La positivité de la PCR augmentait de façon significa- tive avec l'augmentation des taux de séropositivité d'après le test classique d'agglutination en tube et s'élevait à 100 % chez les patients dont les hémocultures étaient positives. (who.int)
Plates1
- The plates go in and the machine analyzes the reactions. (cdc.gov)
Analysis1
- This solution was used for elemental Cr analysis, performed with an ICP-MS device equipped with a concentric nebulizer, a quartz torch with a quartz injector tube, and a cyclonic spray chamber. (cdc.gov)