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  • BBDR
  • BB diabetes-resistant (BBDR) rats develop T1D after immunological perturbations that include regulatory T cell (Treg) depletion plus administration of low doses of a TLR ligand, polyinosinic-polycytidylic acid. (umassmed.edu)
  • In BBDR and control Wistar Furth rats, CD25+ T cells comprised 5-8% of CD4+ T cells. (umassmed.edu)
  • We conclude that CD4+CD25+ cells in the BBDR rat act in vitro and in vivo as natural Tregs. (umassmed.edu)
  • We observed that cells recovered from adult diabetes-resistant BB (BBDR) rat thymi cultured for up to 14 days can adoptively transfer disease to athymic WAG-rnu/rnu rats treated with polyinosinic: polycytidylic acid and a monoclonal antibody to preclude development of ART2a+ regulatory T-cells. (umassmed.edu)
  • cells
  • In vitro, rat CD4+CD25+ T cells were hyporesponsive and suppressed T cell proliferation in the absence of TGF-beta and IL-10, suggesting that they are natural Tregs. (umassmed.edu)
  • It is also known that the development of BB rat T-cells is recapitulated in adult thymus organ cultures (ATOCs). (umassmed.edu)
  • BB rat T-cell lines activated in vitro with antigen-presenting cells (APC) and BB islet cell crude membranes (CM), but not islet cell cytosol, adoptively transfer diabetes into young DP recipients. (umassmed.edu)
  • Ellerman, Karen E. and Like, Arthur A., "Islet cell membrane antigens activate diabetogenic CD4+ T-cells in the BB/Wor rat" (1999). (umassmed.edu)
  • The data suggest that organ-specific autoreactive cells may be present to various degrees but suppressed to a variable extent in many rat strains. (umassmed.edu)
  • In this dissertation, I report a new method for the isolation of rat IELs that is based on the selective removal of intestinal epithelial cells under conditions that leave the basement membrane undisturbed. (umassmed.edu)
  • I also report that rat intraepithelial NK (IENK) and peripheral NK cells are similar in morphology, in their ability to lyse NK-sensitive targets, and in their ability to suppress a one-way mixed lymphocyte culture. (umassmed.edu)
  • I conclude that rat IELs harbor a large population of NKR-P1A + CD3 - cells that function as NK cells but display an activated phenotype and unusual cytokine profile that clearly distinguish them from splenic NK cells. (umassmed.edu)
  • Impaired post-thymic development of regulatory CD4+25+ T cells contributes to diabetes pathogenesis in BB rats. (wikipedia.org)
  • intestinal
  • The rats developed high serum IgE and made IgG autoantibodies that detected a nonleukocytic cell present in the intestinal wall of all rats (including germ free). (ox.ac.uk)
  • vitro
  • T-cell lines activated in vitro with APC and 0.5 mol/l KCl, or pH 11 (0.2 mol/l Na2CO3)-treated islet cell CM, transferred diabetes into young DP rats. (umassmed.edu)
  • conclude
  • We conclude that a major CD4+ T-cell target autoantigen in BB diabetes is a membrane-associated beta-cell molecule with the characteristics of an integral beta-cell membrane protein. (umassmed.edu)
  • disease
  • All rats developed disease, and the median age at humane killing was approximately 36 weeks. (ox.ac.uk)