• Determination of an adequate perfusion pressure for continuous dual vessel hypothermic machine perfusion of the rat liver. (ox.ac.uk)
  • Hypothermic machine perfusion (HMP) provides better protection against ischemic damage of the kidney compared to cold-storage. (ox.ac.uk)
  • Here, we describe an improved supercooling protocol that averts freezing of human livers by minimizing favorable sites of ice nucleation and homogeneous preconditioning with protective agents during machine perfusion. (nature.com)
  • We show that human livers can be stored at -4 °C with supercooling followed by subnormothermic machine perfusion, effectively extending the ex vivo life of the organ by 27 h. (nature.com)
  • Subnormothermic machine perfusion for ex vivo preservation and recovery of the human liver for transplantation: subnormothermic machine perfusion of human livers. (nature.com)
  • To improve the quality of liver grafts from extended-criteria donors donated after circulatory death (DCD), this study explored whether bone marrow mesenchymal stem cells (BMMSCs) combined with normothermic machine perfusion (NMP) have protective effects on DCD donor livers and the effects of ferroptosis in this procedure. (medscimonit.com)
  • Subnormothermic machine perfusion for non-heart-beating donor liver grafts preservation in a Swine model: a new strategy to increase the donor pool? (unipv.it)
  • We show that viability of livers before and after supercooling is unchanged, and that after supercooling livers can withstand the stress of simulated transplantation by ex vivo normothermic reperfusion with blood. (nature.com)
  • Supercooling enables long-term transplantation survival following 4 days of liver preservation. (nature.com)
  • Supercooling preservation and transplantation of the rat liver. (nature.com)
  • Combined Liver, Pancreas-Duodenum, and Kidney Transplantation for Patients with Hepatitis B Cirrhosis, Urem. (annalsoftransplantation.com)
  • HLSC-001 is being evaluated in these patients initially as a bridging therapy prior to liver transplantation with the option and ultimate goal to provide a curative therapy. (unicyte.ch)
  • Intra-portal islet transplantation was performed in diabetic rats as a control. (bvsalud.org)
  • Follow-up after transplantation showed that diabetes was cured in 2/12 rats in the submandibular group in comparison to 4/6 in the control group. (bvsalud.org)
  • However, islet transplantation into rat submandibular glands did not demonstrate a clear advantage over conventional intra-portal transplantation. (bvsalud.org)
  • The purpose of this study is to test the effects of end-ischemic NMP versus end-ischemic HOPE technique in a multicentre prospective randomized controlled clinical trial (RCT) on ECD liver grafts in DBD liver-transplantation (HOPE-NMP). (experimental-surgery.de)
  • In conclusion, our ex vivo model quantifies substrate compartmental distribution and transport across hepatocyte membranes and provides an additional understanding of substrate distribution in the liver. (aspetjournals.org)
  • Electron microscopy showed that the cells in primary culture had a fine structure identical to liver parenchymal cells in vivo, including the observation of desmosomes and bile canaliculi in intercellular space. (nih.gov)
  • Ex vivo modified hepatocytes theoretically may be ready for reinfusion within 100 minutes of liver resection. (elsevierpure.com)
  • In vivo, Y27pPBHSA-treated rats exhibited lower portal pressure, hepatic vascular resistance without effect on systemic vascular resistance, but a tendency towards lower cardiac output compared to non-treated cirrhotic rats. (ibecbarcelona.eu)
  • Extracellular vesicles from human liver stem cells inhibit renal cancer stem cell-derived tumor growth in vitro and in vivo. (unicyte.ch)
  • measurements reflected in vivo situation at each time of liver excision. (biomedcentral.com)
  • In-vivo results in kidney and liver demonstrate we can visualize dynamic changes in gas-induced blood volume changes, as confirmed on laser Doppler perfusion. (ismrm.org)
  • Liver ischemia-reperfusion injury is ameliorated by treatment with various free radical scavengers, as shown in investigations of allopurinol, superoxide dismutase, catalase, and other antioxidant compounds. (ectrx.org)
  • Concordant xenotransplantation of microencapsulated rat islets significantly prolonged mean time of restored normoglycemia (46:t 15 days) in streptozotocin-diabetic BALB/c mice recipients comparing to uncoated grafts (7:t 2 days). (annalsoftransplantation.com)
  • Two-hundred-thirteen (n = 213) human whole organ liver grafts will be submitted to either NMP (n = 85) or HOPE (n = 85) directly before implantation and going to be compared to a control-group of patients (n = 43) transplanted with static cold storage preserved ECD-allografts. (experimental-surgery.de)
  • This study confirmed that sMP was able to resuscitate liver grafts from large NHBD animals. (unipv.it)
  • Adenoviral delivery of superoxide dismutase has been shown to produce beneficial effects in warm ischemia reperfusion (18), alcohol-induced liver injury (19), and reduced-size liver grafts (20). (ectrx.org)
  • Vasuri F, Riefolo M, Ravaioli M, Cescon M, Pasquinelli G, Germinario G, D'Errico A. Predictive value of portal fibrosis and inflammation in transplanted liver grafts treated with hypothermic oxygenated perfusion. (researchprotocols.org)
  • Influence of hepatic arterial blockage on blood perfusion and VEGF, MMP-1 expression of implanted liver cancer in rats. (wjgnet.com)
  • In the laboratory of Gustav Schonfeld, Patsch used rat liver perfusions and primary rat hepatocyte cultures to determine the effects of diets and hormones such as insulin and thyroid hormone on hepatic lipoprotein biosynthesis. (wikipedia.org)
  • To directly assess the effects of the biguanide, metformin, on hepatic gluconeogenesis, it was added at high therapeutic levels (90 pg/ml) to the medium perfusing an isolated rat liver. (diabetesjournals.org)
  • Our results suggest that although pharmacokinetic parameters vary as a function of hepatic cell type, significant intracellular delivery can be readily achieved in the liver after systemic administration. (aspetjournals.org)
  • The disposition of digoxin and the influence of the organic anion transporting polypeptide (Oatp)2 inhibitor rifampicin and the P-glycoprotein (P-gp) inhibitor quinidine on its hepatic disposition were examined in the isolated perfused rat liver. (aspetjournals.org)
  • The effects of hepatic uptake and efflux transporters on metabolism of digoxin were examined in isolated rat hepatocytes versus microsomes. (aspetjournals.org)
  • Rho-kinase (ROCK) activation in hepatic stellate cells (HSC) is a key mechanism promoting liver fibrosis and portal hypertension (PTH). (ibecbarcelona.eu)
  • The main site for butyrate metabolism is the liver since hepatic removal close to 100% has been evidenced in Wistar rats adapted to a high-fiber diet [ 4 ]. (biomedcentral.com)
  • Objective To explore the ability of texture analysis of gadoxetic acid-enhanced magnetic resonance imaging (MRI) T1 mapping images, as well as T1-weighted (T1W), T2-weighted (T2W) and apparent diffusion coefficient (ADC) maps for distinguishing between varying degrees of hepatic fibrosis in an experimental rat model. (cams.cn)
  • Rat livers were preserved in University of Wisconsin organ preservation solution enriched with acridine orange (AO) to stain viable cells and propidium iodide (PI) to detect dead cells. (ox.ac.uk)
  • Our group previously showed that supercooled ice-free storage at -6 °C can extend viable preservation of rat livers 4 , 5 However, scaling supercooling preservation to human organs is intrinsically limited because of volume-dependent stochastic ice formation. (nature.com)
  • Effects of Preservation of Donor Liver Gastroduodenal Artery on Post-Transplant Biliary Complications in 18. (annalsoftransplantation.com)
  • 20°C) is able to improve the preservation of livers obtained from non-heart-beating donors (NHBDs) in rats. (unipv.it)
  • Lembach Jahnsen H, Mergental H, Perera M, Mirza D. Ex-situ liver preservation with machine preservation. (researchprotocols.org)
  • Metabolism of diethylhexyl phthalate by rats: Isolation and characterization of the urinary metabolites. (cdc.gov)
  • The TCPy model includes fat, rapidly perfused, and slowly perfused compartments and compartments from which metabolism of CPF or CPF-oxon leads to TCPy production (brain, diaphragm, liver, and blood). (cdc.gov)
  • Liver microsome incubations are routinely carried out to survey the metabolism of potential drugs by pharmaceutical companies. (aspetjournals.org)
  • This indicated that the liver is highly involved in butyrate metabolism. (biomedcentral.com)
  • In animals, açai supplementation and exercise led to benefits in exercise tolerance and improvements in several hemodynamic parameters, as well as significant improvements in liver markers and glucose metabolism. (bvsalud.org)
  • To investigate whether the major kavalactone kavain imposes adverse effects on the liver ultrastructure and function by affecting vascular and microvascular architecture and altering hepatocellular morphology. (wjgnet.com)
  • BMMSCs showed significant protective effects on the ultrastructure of DCD donor livers and ROS-induced injury to IAR-20 cells under electron microscopy. (medscimonit.com)
  • 1987. Apparent induction of microsomal carboxylesterase activities in tissues of clofibrate-fed mice and rats. (cdc.gov)
  • The autoclavable rodent abdominal organ perfusion chamber is part of our complete systems for the perfusion of rodent isolated organs such as kidney, liver or mesenteric bed from mice, rats or guinea pigs. (harvardapparatus.com)
  • Like the standard Moist Chamber , the Autoclavable Moist Chambe r is intended for perfusion of isolated organs of small animals such as kidney, liver or mesenteric bed of mice, rats or guinea pigs. (harvardapparatus.com)
  • Human liver stem cells express UGT1A1 and improve phenotype of immunocompromised Crigler Najjar syndrome type I mice. (unicyte.ch)
  • For some indications, like colorectal liver metastases, the amount of liver tissue left behind after liver resection has become the main limiting factor of resectability of large or multiple liver tumors. (jove.com)
  • A minimal amount of functional tissue is required to avoid the severe complication of post-hepatectomy liver failure, which has high morbidity and mortality. (jove.com)
  • A 20-minute isolated perfusion using 5 × 10 6 viral particles resulted in expression of the β-galactosidase gene in the rodent livers 72 hours later without histological signs of tissue injury. (elsevierpure.com)
  • Magnetic resonance elastography quantification of the solid-to-fluid transition of liver tissue due to decellularization " was published in the latest issue of the Journal of the Mechanical Behavior of Biomedical Materials . (experimental-surgery.de)
  • Tissue structures in native and decellularized livers were characterized by collagen and elastin quantification, histological analysis, and scanning electron microscopy. (experimental-surgery.de)
  • Our results suggest that the rigidity of liver tissue is largely determined by cellular components, which are replaced by fluid-filled spaces when cells are removed. (experimental-surgery.de)
  • After perfusion fixation, sections were reacted with peroxidase-labeled antibodies to the antireceptor F(ab')2. (nih.gov)
  • Intravital fluorescence microscopy was used to assess liver perfusion by measuring the percentage of AO staining. (ox.ac.uk)
  • This study indicates that fine-tuning of the perfusion pressure is crucial to balance (in)complete perfusion and endothelial injury. (ox.ac.uk)
  • LM, SEM, and TEM examinations indicated kavain-treated rat livers ( n = 4) displayed severe vascular and endothelial damage compared to control livers ( n = 4). (wjgnet.com)
  • Since circulating cells in the liver are in contact predominantly with sinusoidal lining cells (Kupffer cells and endothelial cells), this postulate requires the presence of asialoglycoprotein receptors on the luminal surface of the sinusoidal lining cells. (nih.gov)
  • Constitutive endothelial nitric oxide synthase and inducible nitric oxide synthase are expressed in liver cells. (ectrx.org)
  • Livers from groups of rats were perfused in a recirculatory manner after a bolus dose of digoxin (10 μg), a dual substrate for Oatp2 and P-gp as well as CYP3A. (aspetjournals.org)
  • Perfusions of digoxin were also examined in groups of rats in the presence of the inhibitors: rifampicin (100 μM) or quinidine (10 μM). (aspetjournals.org)
  • Digoxin and its primary metabolite were determined in perfusate and liver by liquid chromatography/mass spectrometry. (aspetjournals.org)
  • Value of Texture Analysis on Gadoxetic Acid-enhanced MR for Detecting Liver Fibrosis in a Rat Model[J].Chinese Medical Sciences Journal, 2019, 34(1): 24-32. (cams.cn)
  • Livers were preperfused with gadopentetate dimeglumine to measure extracellular concentrations. (aspetjournals.org)
  • The hepatocyte-to-extracellular concentration ratio measured at this time point was much higher during MEB perfusion. (aspetjournals.org)
  • 50 transplant centers were included: 318 (66%) kidney or kidney/pancreas, 73 (15.1%) liver, 57 (11.8%) heart, and 30 (6.2%) lung. (tts.org)
  • Since in cirrhosis, platelet-derived growth factor receptor beta (PDGFRβ) is upregulated in the liver as well as the kidney, this study coupled Y27 to human serum albumin (HSA) substituted with PDGFRβ-recognizing peptides (pPB), and investigated its effect on PTH in cirrhotic rats. (ibecbarcelona.eu)
  • Phosphorylation of moesin and myosin light chain (MLC) assessed ROCK activity in liver, femoral muscle, mesenteric artery, kidney and heart. (ibecbarcelona.eu)
  • Such compounds have a potential to accumulate in the blood and can cause toxicity both at the portal of entry and in remote sites such as the liver and kidney (Cruzan et al. (cdc.gov)
  • Subchronic and chronic inhalation studies have shown that these in- haled compounds elicit similar dose-response for toxicity in systemic organs such as the liver and kidney. (cdc.gov)
  • Ischemia-reperfusion injury, which involves complex inflammatory pathways, is a significant cause of liver damage and an important factor in a variety of diseases, injuries, and procedures that include (but not limited to) transplant and trauma. (ectrx.org)
  • Investigations involving animal models and human trials are reviewed, a summary of significant studies in human subjects is presented ( Table 1 ), and the pathways and mediators important in liver ischemia-reperfusion injury are described ( Figure 1 ). (ectrx.org)
  • Designed to perfuse such isolated organs under optimized sterile and physiological conditions, making this chamber suitable for long term perfusion studies over days and weeks. (harvardapparatus.com)
  • Inhaled vapors that are metabolized locally in the respiratory-tract tissues and systemically in the liver and other organs have different dose-response relationships at the portal of entry compared to systemic target organs. (cdc.gov)
  • HSV vectors are potentially powerful tools for gene therapy of human liver disease, because they are efficient and rapid vehicles for gene transfer. (elsevierpure.com)
  • Human Liver Stem Cells: A Liver-Derived Mesenchymal Stromal Cell-Like Population With Pro-regenerative Properties. (unicyte.ch)
  • Intrahepatic Administration of Human Liver Stem Cells in Infants with Inherited Neonatal-Onset Hyperammonemia: A Phase I Study. (unicyte.ch)
  • In this article, the authors found texture feature Entropy of gadoxetic acid-enhanced magnetic resonance imaging T1 mapping images to be a useful biomarker for the diagnosis of liver fibrosis in an experimental rat model. (cams.cn)
  • Methods Liver fibrosis in rats was induced by carbon tetrachloride intraperitoneal injection for 4-12 weeks ( n =30). (cams.cn)
  • METAVIR score was used to grade liver fibrosis as normal (F0), mild fibrosis (F1-2), and advanced fibrosis (F3-4). (cams.cn)
  • Receiver operating characteristic (ROC) curves were obtained to assess the diagnosing accuracy of the parameters for differentiating no liver fibrosis from liver fibrosis and rats with liver fibrosis grading F0-2 from those with grading F3-4. (cams.cn)
  • The pathologic staging of these 20 rats was no fibrosis (F0, n =6), mild fibrosis (F1-2, n =5) and advanced fibrosis (F3-4, n =9). (cams.cn)
  • Entropy and Mean showed a moderate diagnostic performance in most SSF settings of T1 mapping pre-contrast images for differentiation of normal liver from liver fibrosis. (cams.cn)
  • Conclusion Certain texture features of gadoxetic acid-enhanced MR images, especially the Entropy of non-contrast T1 mapping image, was found to be a useful biomarker for the diagnosis of liver fibrosis. (cams.cn)
  • Texture analysis in a rat with mild liver fibrosis (F=1). (cams.cn)
  • It has been postulated that the selective accumulation of circulating desialylated cells in the mammalian liver results from the binding of desialylated glycoproteins on surfaces of the cells to asialoglycoprotein receptors in the liver. (nih.gov)
  • We conclude that it is unlikely that circulating desialylated cells bind to the asialoglycoprotein receptor in the liver unless a breach in the continuity of sinusoidal lining cells exists. (nih.gov)
  • The brown fur was generated from rat cells. (the-scientist.com)
  • Adult rat liver parenchymal cells were isolated by collagenase perfusion and cultured as a primary monolayer in HI/WO/BA, a serum free, completely defined, synthetic culture medium. (nih.gov)
  • BMMSCs also significantly improved the expression level of microtubule-associated protein 1 light chain 3 (LC3)-II in both DCD donor livers and ROS-induced injured IAR-20 cells, including upregulating the expression of ferritin. (medscimonit.com)
  • ECFC were cultured alone or in coculture with rat or human mesenchymal stromal cells (rMSC/hMSC). (experimental-surgery.de)
  • Twenty-four male rat DCD donor livers were randomly and averagely divided into normal, static cold storage (SCS), NMP, and NMP combined with BMMSCs groups. (medscimonit.com)
  • After addition of 25 mg of paracetamol, recovery of sulphate from antegrade perfusions was greater than from retrograde perfusions indicating periportal predominance of this metabolic pathway. (johnshopkins.edu)
  • We conclude that the interplay of transporters and enzymes must be considered in defining the intrinsic metabolic clearance of the liver and in evaluating potential drug-drug interactions. (aspetjournals.org)
  • A major portion of preclinical development involves characterizing the liver enzymes affecting drug disposition and elimination. (aspetjournals.org)
  • Investigation of interleukin-2-mediated changes in blood pressure, fetal growth restriction, and innate immune activation in normal pregnant rats and in a preclinical rat model of preeclampsia. (irapa.org)
  • The effect of therapeutic hypothermia on brain homeostasis in a cardiac arrest rat model. (umaryland.edu)
  • We have compared sMP and standard cold storage (CS) to preserve pig livers after 60 minutes of cardiac arrest. (unipv.it)
  • We used compact magnetic resonance elastography (MRE) with 400, 500, and 600 Hz driving frequency to investigate rat liver specimens for quantification of viscoelastic property changes resulting from decellularization. (experimental-surgery.de)
  • It was used in a series of studies involving perfusions as part of an effort to characterize the effect of a commercial drug candidate on vascular development. (scarletimaging.com)
  • 0.93 ER) in a model of perfused rat liver. (aspetjournals.org)
  • This protocol presents a rat model that allows mechanistic exploration of accelerated regeneration. (jove.com)
  • In this regard, a TCPy PBPK model was developed to link with a validated CPF model to determine the relationship between blood and salivary TCPy in both rats and humans. (cdc.gov)
  • The pharmacokinetics of TCP in blood and urine were previously determined in rats following CPF oral doses of 1, 10 and 50 mg/kg, and the results used to calibrate the TCPy model. (cdc.gov)
  • These findings suggest that the TCPy PBPK model simulates blood and urinary TCPy concentrations following oral exposure to known doses of CPF in both rats and humans. (cdc.gov)
  • The Reduced Uterine Perfusion Pressure (RUPP) rat model of preeclampsia exhibits impaired systolic function and global longitudinal strain during pregnancy. (irapa.org)
  • We then use this model to study the difference in exposure-dose rela- tionship between the respiratory-tract tissues and the liver. (cdc.gov)
  • Liver function, bile secretion, and pathological features of DCD donor livers were detected to evaluate the protective effects of NMP and BMMSCs on DCD donor livers. (medscimonit.com)
  • Perfusion of 1 μM ZnPP abolished detectable levels of CO in the venous perfusate and increased bile acid-dependent bile output accompanying an increased secretion of bile salts. (elsevierpure.com)
  • 1978. Short-term oral toxicity study of diethyl phthalate in the rat. (cdc.gov)
  • Identification of the metabolites of simple phthalate diesters in rat urine. (cdc.gov)
  • Then, we transplanted 2600 islet equivalents into the submandibular glands of diabetic Lewis rats. (bvsalud.org)
  • Y27pPBHSA reduced intrahepatic resistance by reduction of phosphorylation of moesin and MLC in Y27pPBHSA-treated cirrhotic rats. (ibecbarcelona.eu)
  • Butyrate, end-product of intestinal fermentation, is known to impair oxidative phosphorylation in rat liver and could disturb glycogen synthesis depending on the ATP supplied by mitochondrial oxidative phosphorylation and cytosolic glycolysis. (biomedcentral.com)
  • More recently, we demonstrated in isolated rat liver that a butyrate perfusion of the organ decreases the oxidative phosphorylation yield in the whole organ by decreasing the mitochondrial synthesis flux of ATP [ 9 ]. (biomedcentral.com)
  • Recent evidence has shown that in cases of generalized ischemia after hemorrhagic shock, the effectiveness of melatonin in improving liver function depends on the melatonin receptor (40). (ectrx.org)
  • 7 A novel procedure inducing liver regeneration at a similar pace as after partial hepactectomy was developed by a group of surgeons who discovered that adding a transection between the occluded and the non-occluded part of the liver induces liver hypertrophy at the same growth rate as after liver resection, but prior to resection. (jove.com)
  • Inducing rapid liver hypertrophy using Associating Liver Partition and Portal vein ligation for a Staged hepatectomy (ALPPS) has been proposed for resection of borderline resectable liver tumors. (jove.com)
  • Inducing liver growth of the prospective remnant prior to resection has become more established in liver surgery, either in the form of portal vein embolization by interventional radiologists or in the form of portal vein ligation several weeks prior to resection. (jove.com)
  • Recently, it was shown that liver regeneration is more extensive and rapid, when the parenchymal transection is added to portal vein ligation in a first stage and then, after only one week of waiting, resection performed in a second stage (Associating Liver Partition and Portal vein ligation for Staged hepatectomy = ALPPS). (jove.com)
  • 2 This post-hepatectomy liver failure is the most devastating complication after liver resection. (jove.com)
  • The time necessary for the liver to grow is weeks to months even though the liver can regenerate at a much faster rate after resection. (jove.com)
  • 9 The procedure initiates rapid hypertrophy of 80% within a week in the future liver remnant, which allows the resection of extensive, primarily unresectable, liver tumors within a week. (jove.com)
  • Chan LK and Ng IO: Joining the dots for better liver cancer treatment. (spandidos-publications.com)
  • Livers treated with NMP combined with BMMSCs showed better liver function, relieved histopathological damage, reduced oxidative stress injury and ferroptosis, and the mechanism of reduction was associated with downregulation of intracellular reactive oxygen species (ROS) and free Fe²⁺ levels. (medscimonit.com)
  • We previously showed that the direct perfusion of isolated and perfused rat liver with short and medium chain FA (butyrate, octanoate) decreased the ATP content [ 8 ]. (biomedcentral.com)
  • Decellularization did not affect the integrity of microanatomy and structural composition of liver ECM but was found to be associated with increases in the relative amounts of collagen by 83-fold (37.4 ± 17.5 vs. 0.5 ± 0.01 μg/mg, p = 0.0002) and elastin by approx. (experimental-surgery.de)
  • 6- 3 H]glucose was added at the beginning of the perfusion in studies where [ 3 H]lactate was not infused. (diabetesjournals.org)
  • This was corroborated by measurement of labeled lactate extraction as well as glucose, CO 2 , and lactate production across the liver. (diabetesjournals.org)
  • Mitochondrial ATP turnover, calculated after specific inhibition of glycolysis, was stable (~0.70 ± 0.25 μmol.min -1 .g -1 liver ww) during the first two hr whatever the force-feeding, and increased transiently about two-fold at the 3rd hr in glucose. (biomedcentral.com)
  • In liver from 48 hr-starved rats, the energy need for net glycogen synthesis from exogenous glucose corresponds to ~50% of basal mitochondrial ATP turnover. (biomedcentral.com)
  • The liver is the main site of glycogen contribution to the regulation of blood glucose. (biomedcentral.com)
  • Glycogen content of the liver fell by 60% relative to control and was greater for the gluconeogenic pathway. (diabetesjournals.org)
  • A net linear flux of glycogen synthesis (~11.10 ± 0.60 μmol glucosyl units.h -1 .g -1 liver wet weight) occurred until the 6th hr post-feeding in both groups, whereas butyrate delayed it until the 8th hr. (biomedcentral.com)
  • For this reason, we examined the intracellular fate of ISIS 1082, a 21-base heterosequence phosphorothioate oligodeoxynucleotide, isolated from parenchymal and nonparenchymal cell types after systemic dosing using established perfusion and separation techniques followed by CGE. (aspetjournals.org)