• In the early 90s, several groups identified in murine brain extracts, a protein of a molecular weight between 100-160 kDa, named Ras-GRF (Ras-Guanine nucleotide Releasing Factor) and Cdc25Mm, based on its ability to induce GDP release in p21ras and on its high homology with the Sacharomyces cerevisiae gene CDC25, whose deficiency it could rescue. (atlasgeneticsoncology.org)
  • Recently, expression and post-translational modification of Ras guanine nucleotide releasing factor 1 (RasGRF1) was found to contribute to spontaneous MMP production in melanoma cancer cells. (biomedcentral.com)
  • These results validate LCM as a reliable technique for in vivo genomic studies in the brain hippocampus, where contamination by surrounding areas (not expressing Ras-GRF1) increases background noise and impairs identification of differentially expressed genes. (omicsdi.org)
  • Comparison between wild type and knockout LCM hippocampus samples revealed that Ras-GRF1 elimination caused significant gene expression changes, mostly affecting signal transduction and related neural processes. (omicsdi.org)
  • Keywords: knockout analysis Overall design: 6 samples of tissue sections of the hippocampus area of wild type (WT) and Rasgrf1 knockout (KO) mice brains, 3 biological replicates of each. (omicsdi.org)
  • The RAS proteins in turn signal through six alternative effector families to affect many downstream targets including TP53, RB1, and MTOR. (cancer.gov)
  • He also included RGL, RGL2 and RGL3 with RALGDS as another effector that links RAS with RAL. (cancer.gov)
  • The RasGrf proteins exhibit modular structures composed by multiple domains including CDC25H and DHPH motifs responsible for promoting GDP/GTP exchange, respectively, on Ras or Rho GTPase targets. (nih.gov)
  • At the top of the diagram are the receptors and messengers to the three RAS proteins. (cancer.gov)
  • Gaddy Getz and Matt Myerson and colleagues at the Broad Institute along with Bob Stephens, Jim Hartley and colleagues at the Frederick National Laboratory for Cancer Research are utilizing this diagram to develop a RAS-centric version of The Cancer Genome Atlas (TCGA). (cancer.gov)
  • Fibroblast-like synoviocytes (FLS) from rheumatoid arthritis (RA) patients share many similarities with transformed cancer cells, including spontaneous production of matrix metalloproteinases (MMPs). (biomedcentral.com)
  • Upon neurotrophin binding, Trk associates in neurons with a number of signaling proteins including the Shc, SNT/FRS-2, and APS/SH2-B adapter proteins and regulators of Ras function, the calcium and PKC regulator phospholipase C-γ1, and the phosphotyrosine phosphatase SHP-1. (sigmaaldrich.com)
  • These proteins link Trk to the Ras/Raf/MEK/MAP kinase, Rap1/Braf, PI3-kinase/Akt/GSK3-β/ILK, ΔNp73 (p53 family member) and PKC signaling pathways. (sigmaaldrich.com)
  • Preferentially activates HRAS in vivo compared to R-RAS based on their different types of prenylation. (uniprot.org)