MutagenesisMutagenesis, Site-DirectedDirected Molecular EvolutionMolecular Sequence DataMutagenesis, InsertionalAmino Acid SequenceMutationEscherichia coliAmino Acid SubstitutionModels, MolecularBase SequenceProtein EngineeringGene LibraryEnzyme StabilityLuciferases, BacterialBinding SitesBacterial ProteinsPoint MutationPlasmidsStructure-Activity RelationshipProtein Structure, TertiaryDNA Transposable ElementsKineticsRecombinant ProteinsEthylnitrosoureaSubstrate SpecificityCloning, MolecularEscherichia coli ProteinsProtein Structure, SecondaryPolymerase Chain ReactionSequence Homology, Amino AcidMutant ProteinsDNA PrimersProtein ConformationGenes, BacterialCatalysisDNA Mutational AnalysisSequence AlignmentSaccharomyces cerevisiaeSubtilisinProtein BindingPhenotypeGenetic Complementation TestGene Expression Regulation, BacterialMutation, MissenseCysteine3-Isopropylmalate DehydrogenaseSequence Analysis, DNATemperatureConserved SequenceIndustrial MicrobiologyAspartic AcidGenetic EngineeringCatalytic DomainDNA, BacterialSuppression, GeneticTransformation, GeneticPeptide LibraryGenetic TechniquesPseudomonas putidaSaccharomyces cerevisiae ProteinsCell LineSubtilisinsRecombinant Fusion ProteinsSequence DeletionValineRecombination, GeneticHot TemperatureOligodeoxyribonucleotidesFungal ProteinsCodonGenetic VectorsRestriction MappingDNA-Binding ProteinsPromoter Regions, GeneticAllelesAntibody AffinityTransfectionHydrogen-Ion ConcentrationDNALuminescent ProteinsGene DeletionTwo-Hybrid System TechniquesLeucineCarrier ProteinsArginineGenes, ReporterTranscription, GeneticAmino AcidsBacillus subtilisSelection, Geneticbeta-GalactosidaseGenes, DominantMolecular StructureCell MembraneMembrane Transport ProteinsVirulenceProtein FoldingTranscription FactorsGene Expression