To investigate the Ns genome donor of Leymus and to elucidate the origin of Xm genome of Leymus , Random Amplified Polymorphic DNA (RAPD) markers were used to analyze 13 Leymus accessions, together with 14 taxa from related Triticeae genera. (degruyter.com)
A total of 156 clear and reproducible DNA fragments were amplified by 24 RAPD primers, among which 154 fragments (98.72%) were found to be polymorphic. (degruyter.com)
To identify DNA-based genetic polymorphism for constructing a genetic linkage map of catfish, we tested 100 random amplification of polymorphic DNA (RAPD) primers for their utility in identifying genetic polymorphism in catfish. (syr.edu)
and 25 produced poorly reproducible RAPD profiles or non-polymorphic RAPD profiles. (syr.edu)
have been studied by RAPD (random amplified polymorphic DNA) and statistical cluster analysis. (jipb.net)
One approach to genetic diversity uses molecular markers, namely Random Amplified Polymorphic DNA (RAPD) markers, which are universal and more economical markers using primers to amplify random DNA sequences to detect the presence of a DNA polymorphism. (rroij.com)
Thus, the Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technique was used to examine the genetic variability among individuals of S . olivacea from Setiu Wetlands, Terengganu. (mabjournal.com)
Twenty reproducible RAPD fragments generated by OPA 08 and OPA 10 with 17 fragments were polymorphic. (mabjournal.com)
Application of Random Amplified Polymorphic DNA (RAPD) in Molecular Ecology. (mabjournal.com)
Stevens, J. & Wall, R. (1995) The use of random amplified polymorphic DNA (RAPD) analysis for studies of genetic variation in populations of the blowfly Lucilia sericata in southern England. (exeter.ac.uk)
Six Random Amplified Polymorphic DNA (RAPD) markers linked to high sugars were found, five dominant and one co-dominant. (usda.gov)
The diversity within and between the two hemp cultivars "Kompolti" and "Fasamo" was examined with 18 RAPD (Random Amplified Polymorphic DNA) primers. (gwdg.de)
Isolates (50) of E. coli obtained from liquid manure (20 bovine, 20 porcine) were genotyped using random amplified polymorphic DNA (RAPD). (iwaponline.com)
A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. (scielo.br)
Random Amplified Polymorphic DNA (RAPD) analysis was carried out to study genomic changes using 10 different random primers. (cdc.gov)
The genetic diversity of 10 diploid and tetraploid wheat species was estimated using random amplified polymorphic DNA (RAPD) markers. (tubitak.gov.tr)
Random amplified polymorphic DNA (RAPD) is one of the easiest and most commonly used molecular techniques for genetic variability analysis. (tubitak.gov.tr)
Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) dominant markers were used to assess genetic variability in the aphid Sitobion avenae (Fabricius) in relation to hydroxamic acid levels in their host-plants. (cambridge.org)
The genetic variability of the isolates was first assessed using Random Amplified Polymorphic DNA (RAPD) markers. (bvsalud.org)
RAPD (Random Amplified Polymorphic DNA) markers have been most widely used for this purpose 8-10 . (bvsalud.org)
Markers2
Therefore, we tested the transferability of 12 simple sequence repeats from expressed sequence tags (EST-SSRs) from Catharanthus roseus to H. speciosa and used 10 random amplified polymorphic DNA markers to evaluate the genetic variability among botanical varieties of H. speciosa. (unesp.br)
Amplified fragment length polymorphism (AFLP) markers were used to detect the relationships among 12 wild accessions and 62 groundcover chrysanthemum cultivars. (ashs.org)
Primers3
The 75 high and medium quality primers generated 462 polymorphic bands, an average of 6.1 bands per primer. (syr.edu)
A total of 112 DNA fragments were generated by the 15 random primers, with an average of about 7.4 bands per primer. (geneticsmr.com)
Twenty two random primers with an average of 71.2% polymorphism produced 151 polymorphic bands. (scirp.org)
DNAs2
Inhibitation of Random Amplified Polymorphic DNAs (RAPDs) by Plant Polysaccharides. (forda-mof.org)
Das Ziel der vorliegenden Arbeit war einerseits die Untersuchung der genetischen Diversität bei der diözischen Sorte "Kompolti" und der monözischen Sorte "Fasamo" mittels Random Amplified Polymorphic DNAs (RAPDs) und andererseits die Etablierung der Fasergehaltsanalyse sowie die Bestimmung und Auswertung der Fasergehalte von Hanfformen. (gwdg.de)
The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. (scielo.br)
Sequences1
random amplified polymorphic DNA analysis and mitochondrial DNA sequences. (exeter.ac.uk)
Detect1
Failure to detect an allele within a sample or failure to amplify an allele during PCR. (ojp.gov)
Strains1
Effect of Development Stage on the Artemisinin Content and the Sequence Characterized Amplified Region (SCAR) Marker of High-Artemisinin Yielding Strains of Artemisia annua L. [J]. J Integr Plant Biol. (jipb.net)
Primer1
Nineteen EcoR I/ Mse I primer combinations revealed 452 informative polymorphic bands with a mean of 23.8 bands and 71.5% polymorphic rate per primer pair. (ashs.org)
Bands1
The percentage of polymorphic bands was 85% indicates that the population of S. olivacea has high level of polymorphism due to low inbreeding factor within a population. (mabjournal.com)
Randomly amplified3
Plasmid profiles and randomly amplified polymorphic DNA analysis of Salmonella enterica serotype Enteritidis strains from outbreaks and sporadic cases in Turkey. (nih.gov)
20. FluoMEP: a new genotyping method combining the advantages of randomly amplified polymorphic DNA and amplified fragment length polymorphism. (nih.gov)
Using the 14 primers, a total of 389 DNA sections were randomly amplified, indicating an average of 27.7 DNA sections amplified by each primer. (ncl.edu.tw)
Polymorphism1
Recently random primers have been used to detect DNA polymorphism. (genelink.com)
Fragment3
1990) and amplified fragment length polymorphisms (AFLPs) (Vos et al . (scialert.net)
4. Genetic alterations of hepatocellular carcinoma by random amplified polymorphic DNA analysis and cloning sequencing of tumor differential DNA fragment. (nih.gov)
A D-loop fragment of approximately 330 bp was amplified by PCR. (geneticsmr.com)
Polymerase chain re2
The characterization and comparison of Staphylococcus aureus by antibiotic susceptibility testing, enterobacterial repetitive intergenic consensus-polymerase chain reaction, and random amplified polymorphic DNA-polymerase chain reaction. (nih.gov)
Diagnosis by random amplified polymorphic DNA polymerase chain reaction of four cryptic species related to Anopheles (Nyssorhynchus) albitarsis (Diptera: Culicidae) from Paraguay, Argentina, and Brazil. (psu.edu)
ISSR1
The genetic variations accompanied with in vitro conditions of callus induction was evaluated by four primers of inter-simple sequence repeat (ISSR) that amplified 36 bands. (springeropen.com)
Genome1
Certain properties are desirable for a molecular marker such as highly polymorphic behavior, codominant inheritance, frequent occurrence in the genome, even distribution throughout the genome, selectively neutral behavior, easy access, easy and fast assay and high reproducibility (Weining and Henry, 1995). (scialert.net)
Genetic3
These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. (genelink.com)
The degree of genetic divergence was estimated in seven wheat genotypes, six exotic genotypes and one local variety, through random amplified polymorphic DNA methodology. (geneticsmr.com)
Ten maize accessions (NC-9, A50-2, M-14, B-42, NC-3, T-7, N-48-1, B-34, USSR, and WFTMS) were studied to estimate the genetic distance on molecular level by random amplified polymorphic DNA. (geneticsmr.com)
Genomic3
9. Application of random amplified polymorphic DNA PCR for genomic analysis of HIV-1-infected individuals. (nih.gov)
14. Detection of amplified genomic sequences in human small-cell lung carcinoma cells by arbitrarily primed-PCR genomic fingerprinting. (nih.gov)
Detection of genomic instability in lung cancer tissues by random amplified polymorphic DNA analysis. (cdc.gov)
Fragments were generated1
A total of 248 fragments were generated and 139 bands were polymorphic at the inter-specific level. (scialert.net)
Analysis3
random amplified polymorphic DNA analysis and mitochondrial DNA sequences. (exeter.ac.uk)
Tachyzoite harvests obtained during continuous cell culture were retrieved from liquid nitrogen and cultured in HeLa cells, providing mRNA that was extracted and used to study gene expression using random amplified polymorphic DNA analysis at different stages of lineage adaptation to continuous culture. (bmj.com)
The present invention provides a method of arbitrary sequence oligonucleotide fingerprinting (ASOF), a novel technology which eliminates gel electrophoresis as a step in polymorphic marker analysis, species identification and transcriptional profiling. (justia.com)
Gene1
16. The novel human MOST-1 (C8orf17) gene exhibits tissue specific expression, maps to chromosome 8q24.2, and is overexpressed/amplified in high grade cancers of the breast and prostate. (nih.gov)
Studies1
Random prime labeling yields high specific activity labeled DNA probe which can be used for all southern, northern and in situ hybridization studies. (genelink.com)