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  • galactosyl
  • The raffinose-complex reveals a conserved mode of donor substrate binding, involving minimal contacts with the raffinose galactosyl unit, which protrudes out of the active site, and specificity-determining contacts essentially restricted to the sucrosyl moiety. (nih.gov)
  • The present raffinose complex reinforces a view that donor substrate recognition in B. subtilis levansucrase rests primarily on the common sucrosyl unit, whereas the galactosyl moiety, which protrudes out of the active site, makes only a few water-mediated H-bonds, pointing three unliganded hydroxyl groups to the bulk solvent. (nih.gov)
  • mutants
  • Here, we report high-resolution structures of three levansucrase point mutants, D86A, D247A, and E342A, and that of raffinose-bound levansucrase-E342A. (nih.gov)
  • We present here the raffinose-bound complex of levansucrase-E342A in addition to the apo crystal structures of the three inactive point mutants D86A, D247A and E342A. (nih.gov)
  • family
  • This mode of binding is echoed by the raffinose-bound complex of Thermotoga maritima invertase, which belongs to GH family 32 and which, like levansucrase, mediates hydrolysis of the glycosidic bond through a double displacement reaction mechanism . (nih.gov)
  • found
  • In thylakoid membrane of chloroplasts isolated from spinach ( Spinacia oleracea ) leaves under freezing, drought, or heat stress conditions, raffinose has been found to reduce the inactivation of the electron (2,6-dichlorophenolindophenol reduction) and cyclic photophosphorylation in photosynthesis ( Santarius, 1973 ). (plantphysiol.org)
  • whole
  • Whole cell and surface proteomes were analyzed together with adhesive properties of the probiotic bacterium Lactobacillus acidophilus NCFM (NCFM) grown on the emerging prebiotic raffinose, exemplifying a synbiotic. (dtu.dk)