• For example, receptor functional assays, which measure GPCR downstream signaling effectors, 3 are inadequate for identifying allosteric or biased signaling modulators and often generate hits unsuitable for subsequent optimization. (rsc.org)
  • Further pharmacological experiments in a [35S]GTPγS binding assay using hA3-CHO-cells indicated that PSB-10 acts as an inverse agonist (IC50 = 4 nM). (wikipedia.org)
  • Thus reading of an assay may be quite noisy and may involve greater difficulties in interpretation than an accurate chemical titration. (wikipedia.org)
  • One of the more powerful methods for this purpose has been the radioligand binding assay (Yamamura et al, 1978). (springer.com)
  • The thermofluor assay was the first high-throughput thermal shift assay and its utility and limitations has spurred the invention of a plethora of alternate methods. (wikipedia.org)
  • Whether the chelate dissociated and resulted in 64Cu binding to the protein superoxide dismutase (SOD) in rat liver was investigated by utilizing a gel electrophoresis assay for the detection of SOD. (wikipedia.org)
  • Or else, since I think the drug could be binding to a site distinct from the agonist binding site, could it be that the drug alters the receptor conformation which also changes the configuration of the agonist binding site and so prevents radioligand binding? (scientistsolutions.com)