• Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA). (wikipedia.org)
  • Thirty-eight samples were subtyped for d and y determinants by radioimmunoprecipitation assay (RIPA) and the polymerase chain reaction (PCR). (ox.ac.uk)
  • If results were positive, a confirmatory radioimmunoprecipitation assay (RIPA) was performed. (cdc.gov)
  • For these reasons, the Public Health Service has emphasized that an an individual be consideredto have serologic evidence of HIV infection only after an enzyme immunoassay (EIA) screening test is repeatedly reactive* and another test such as Western blot (WB) or immunofluorescence assay has been performed to validate the results (1). (cdc.gov)
  • Some laboratories also use radioimmunoprecipitation assays and indirect immunofluorescence assays. (cdc.gov)
  • We used histologic, immunofluorescence, RNA sequencing, and metabolic assays to characterize kidneys from these mice and controls during development and up to postnatal day 21. (lww.com)
  • HUVECs were administrated with TMZ or TMZ and lentivirus, the expression of EndMT and related proteins was observed by wound healing assay, immunoblotting, and immunofluorescence. (dovepress.com)
  • The miRNA-mRNA interactions were verified by dual-luciferase reporter gene assays and experiments using mimics miRNA or their inhibitors. (frontiersin.org)
  • Typically autoantibodies have already been discovered by radioimmunoprecipitation assays (RIA) or in some instances by enzyme-linked immunosorbent assays (ELISA) or fluorescence immunoprecipitation assays (FIPA). (hwupdate.org)
  • Molecular mechanisms and the influences of different regulation the expression of AKT1 on HCC cell growth, proliferation were determined by western blotting, MTT and colony formation assays, cell cycle and apoptosis were investigated by flow cytometry. (spandidos-publications.com)
  • Cell viability assays, colony formation assays, wound healing assays, and Transwell assays demonstrated that ebastine elicited antitumor effects in osteosarcoma cells. (ijbs.com)
  • Cell migration and invasion was monitored by transwell chambers assay. (ijpsonline.com)
  • In addition, after the overexpression or knockdown of PAK3 in cells, the proliferation, migration and invasion abilities of these cells were assessed by Cell Counting Kit-8 (CCK-8), wound healing and Transwell assays, and the results were confirmed in vivo experiments in mice. (jcancer.org)
  • The binding sites of miR-128-3p and TGFBR2 were predicted by Targetscan online software and confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. (degruyter.com)
  • These assays could be delicate and particular extremely, but usually do not detect one of the most pathogenic antibodies always. (hwupdate.org)
  • Lately cell-based assays (CBAs) have already been established to be able to search for antibodies that bind towards the extracellular domains of protein that are normally expressed over the cell surface area. (hwupdate.org)
  • Serum examples Serum examples that were examined had been either archived SNMG who had been negative on a typical radioimmunoprecipitation assays for recognition of autoantibodies to either AChR and MuSK or from sufferers with a scientific diagnoses of MG delivered to Oxford to become analysed by CBAs for clustered AChR or MuSK antibodies (n = 415). (hwupdate.org)
  • A cell-based assay for the recognition of anti-COLQ antibodies To identify anti-COLQ antibodies, HEK293 cells transfected using the build encoding COLQ-CASPR2TM had been incubated with serum examples and Alexa Fluor 568 anti-human IgG supplementary antibodies. (hwupdate.org)
  • The assay was utilized to display screen 415 MG sera for the current presence of antibodies to COLQ. (hwupdate.org)
  • The full total results of these assays weren't known during testing for COLQ antibodies. (hwupdate.org)
  • 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay was executed for determination of effects of piperine on breast cancer cell viability. (ijpsonline.com)
  • T. cruzi screening was performed by indirect hemagglutination assay. (cdc.gov)
  • Apoptosis was checked by acridine orange/ethidium bromine staining assay and by execution of Western blotting analysis. (ijpsonline.com)
  • Acridine orange/ethidium bromine staining assay revealed that piperine induced apoptosis in MCF-7 cells which was further investigated by Western blotting. (ijpsonline.com)
  • Western blotting assay was implemented to check the levels of phosphatidylinositol 3-kinase and protein kinase B. Results revealed that piperine induced dose reliant cytotoxicity in MCF-7 human breast cancer cells. (ijpsonline.com)
  • Since licensure of the first EIA test kits in 1985, the manufacturers have worked to improve the sensitivity, specificity, and reproducibility of their assays. (cdc.gov)
  • Clonogenic assay was implemented for checking the effect of piperine on clonogenic potency of breast cancer cells. (ijpsonline.com)
  • Children confirmed as positive by serum assays underwent endoscopy with duodenal biopsies and, at the diagnosis of CD, were suggested to start GFD. (foodsmatter.com)
  • Der f 1) and cat (Fel d 1) allergens measured by isotype (IgG allergen do not adequately reflect exposure of the and IgG4) specific radioimmunoprecipitation assays were respiratory tract. (cdc.gov)
  • Finally, Western blotting assay predicted constant expression of phosphatidylinositol 3-kinase and protein kinase B and reduced expression of phosphorylated phosphatidylinositol 3-kinase. (ijpsonline.com)
  • Four thousand forty-eight saliva samples were tested for anti-tissue transglutaminase (tTG) immunoglobulin (Ig)A using a fluid-phase radioimmunoprecipitation method. (foodsmatter.com)
  • In vitro antifungal assays showed that rosmarinic acid had a favorable inhibitory effect on fungi. (biomedcentral.com)
  • The inhibitory effects of bufalin on cell proliferation were determined by MTT (Methyl thiazolyltetrazolium) assay. (biomedcentral.com)
  • both chromatin immunoprecipitation and electrophoretic mobility shift assay showed predominant Smad3 binding in the +51 to +78 bp region of NRP2′s 5′ untranslated region. (ahajournals.org)
  • The antigen-antibody complex was captured on protein G beads, washed four times with radioimmunoprecipitation assay buffer, and then washed with phosphate-buffered saline. (chip-atlas.org)
  • Sarpogrelate hydrochloride MEF3T3-275 and MEF3T3-275-3-2 cells were washed twice with ice-cold PBS and incubated with radioimmunoprecipitation assay buffer (Merck) for 10 min on ice and centrifuged for 5 min at 9000test in GraphPad Prism 6 (GraphPad Software). (epf2013.org)
  • dual-luciferase reporter assay was used to detect the targeting relationship between circ_0000419 and microRNA-224. (ijpsonline.com)
  • Luciferase reporter assay exhibited that miR-146b-3p inhibited the luciferase activity of PTPN12 markedly. (degruyter.com)
  • Only 2 scFvs recognizing PA (W1 and W2) neutralized the cytotoxicity of lethal toxin in a macrophage lysis assay. (nih.gov)
  • Several independent studies compared the original immunohistochemistry assay with newly developed recombinant assays, and consistently found a higher sensitivity with the recombinant tests. (medscape.com)
  • [ 90 ] Their results were, however, based on the least sensitive assay (immunohistochemistry), and a nonstandard starting serum dilution was used. (medscape.com)
  • miR-146b-3p inhibitor and mimic were transfected, followed by qRT-PCR and WB assays to detect the expression of PTPN12. (degruyter.com)
  • Antigens in preparations of simian sarcoma virus/simian sarcoma-associated virus reacting with human sera in radioimmunoprecipitation assays. (nih.gov)
  • Moreover, there was little cross-reactivity when assayed against 246 sera from non-chagasic individuals, including 105 samples from individuals infected with unrelated trypanosomatids. (ajtmh.org)
  • The assays employ various different immunological techniques and their sensitivity varies broadly, but all confirm a high specificity of the antibody for NMO. (medscape.com)
  • Given the low sensitivity of some assays, repeat testing in patients with an initially negative test result seems justified, especially if the initial sample was taken during remission or under immunosuppressive treatment. (medscape.com)
  • Since licensure of the first EIA test kits in 1985, the manufacturers have worked to improve the sensitivity, specificity, and reproducibility of their assays. (cdc.gov)
  • Cell activity was detected by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay. (ijpsonline.com)
  • MTT assay and Oil Red O staining revealed that RSG ameliorated the PA-induced decrease in TM4 cell viability, which was accompanied by an alleviation of PA-induced lipid accumulation in cells. (biomedcentral.com)
  • OB cells viability was detected by cell viability assay. (biomedcentral.com)
  • In addition, one case reported in 1991 was accepted based only on a positive radioimmunoprecipitation assay (a now obsolete test). (cdc.gov)
  • We utilized a sphere culture model, a soft agar assay, and soft and rigid hydrogel surface culture models to investigate the function of rigidity sensing in the tumorigenicity of OS cells. (nature.com)