Radiation hybrid mapping. Medical search. FAQ

Radiation hybrid (RH) mapping is based on radiation-induced chromosome breakage and analysis of chromosome segment retention or loss using molecular markers. (genetics.org)
The analysis of 87 individuals of this population with 39 molecular markers mapped on chromosome 1D revealed 88 radiation-induced breaks in this chromosome. (genetics.org)
The analysis of molecular marker retention in our radiation hybrid mapping panel allowed the localization of scs ae and 8 linked markers on the long arm of chromosome 1D. (genetics.org)
We describe here the integration of an expanded canine radiation hybrid map, comprised of 600 markers, with the latest linkage map of 341 markers, to generate a map of 724 markers-the densest map of the canine genome described to date. (nih.gov)
Through the inclusion of 217 markers on both the linkage and RH maps, the 77 RH groups are reduced to 44 syntenic groups, thus providing comprehensive coverage of most of the canine genome. (nih.gov)
The boxed markers on the deletion map refer to sequences assigned to a specific region on chromosome 1D. (genetics.org)
The RH map has an estimated total length of 11,737 cR 35,000 covered by 378 AFLP (marked in blue), EST, RFLP, and SSR (all marked in red) markers. (genetics.org)
Markers in this region cover the majority of the long arm recombination-based linkage map (left, centimorgan distances). (genetics.org)
Markers are organized according to their position on the RH map and critical breaks (shaded boxes) are clearly evident. (genetics.org)
Molecular markers used in developing wheat consensus genetic maps were used on the DWRH-1D panel and the distances between them (in centimorgans) were calculated on the basis of genetic maps. (genetics.org)
The physical distances between markers (in megabases) were calculated on the basis of estimated resolution of the comprehensive RH map. (genetics.org)
This significantly hampers ordering of markers, contigs of physical maps and sequence scaffolds and impedes obtaining of high-quality reference genome sequence. (k-state.edu)
A total of 2316 and 2695 markers were successfully mapped to the 4A RH and deletion maps, respectively. (k-state.edu)
Markers included in both this RH map and the bovine cytogenetic map were in a consistent order. (deepdyve.com)
Effective utilization of the domestic cat as an animal model for hereditary and infectious disease requires the development and implementation of high quality gene maps incorporating microsatellites and conserved coding gene markers. (semanticscholar.org)
In an effort to develop maps for the microchromosomes absent from the chicken genome assembly, we developed radiation hybrid (RH) and genetic maps with markers isolated from sequence currently assigned to 'chromosome Unknown' (chrUn). (le.ac.uk)
Results:In total, 139 markers were analysed with the chickRH6 panel, of which 120 were effectively assigned to the E26C13 linkage group, the remainder mapping elsewhere in the genome. (le.ac.uk)
The final RH map is composed of 22 framework markers extending over a 245.6 cR distance. (le.ac.uk)
Because the average fragment length is known, these maps determine relative distance between two markers. (brainkart.com)
A total of 92 simple sequence repeat markers were genotyped onto the mapping panel for G. barbadense. (tamu.edu)
Data from 64 of the 92 markers were deemed robust and combined with pre-existing data to develop an expanded framework map, which provides partial coverage of 7 chromosomes and three unidentified linkage groups. (tamu.edu)
Placement of a group of 235 unbiased markers on the RH map suggests that the map generated for the LN54 panel, at present, covers 88% of the zebrafish genome. (elsevier.com)
A total of 57 markers were developed based on synteny or literature and genotyped to produce a RH map spanning 205.2 cR. (cgiar.org)
Moreover, the hybrids are easier to analyze with markers from multigenic families due to their reduced complexity. (uthscsa.edu)
Moreover, once framework RH maps of a genome have been constructed, a quick location of markers by simple PCR on the RH panel is possible. (core.ac.uk)
To confirm the validity of the map and to provide valuable comparative mapping information, both markers from the genetic map and a high number of ESTs (Expressed Sequence Tags) were used. (core.ac.uk)
Conclusion - The high resolution framework map obtained in this study has markers covering the entire chicken chromosome 5 and reveals the existence of a high number of rearrangements when compared to the human genome. (core.ac.uk)
Microsatellite markers from the genetic map were used to check the validity of the RH map and additional markers were developed from chicken EST data to yield comparative mapping data. (inra.fr)
Currently, the canine map is composed of about 400 such markers, which, in principal, provides "addresses" for about 75 percent of the genome. (akcchf.org)
This proposal aims to identify numerous additional markers and place them on the canine genome map. (akcchf.org)
These markers have been mapped using either genetic mapping (Genethon, Marshfield, and deCODE maps), radiation hybridization mapping (Stanford, Whitehead RH, and GeneMap99 maps) or YAC mapping (the Whitehead YAC map) techniques. (ucsc.edu)
radiation hybrid map markers are shown in black. (ucsc.edu)
Sequence and primer information for the markers were obtained from the primary sites for each of the maps, and from NCBI UniSTS (now part of NCBI Probe ). (ucsc.edu)
en] The callipyge (CLPG) gene was fine-mapped by linkage analysis to a 4.6-cM chromosome interval on distal ovine OAR18q, flanked by microsatellite markers IDVGA30 and OY3. (ac.be)
o To develop and map SSR markers and to use them to anchor BACs. (bio.net)
At the whole-genome level, 26 299 SNP markers were mapped on the RH panel and provided an average mapping resolution of approximately 248 Kb/cR₁₅₀₀ with a total map length of 6866 cR₁₅₀₀. (oregonstate.edu)
They blasted the genome into fragments, typed 5,255 markers among the pieces, and then assembled them, creating a physical map. (the-scientist.com)
A reference map of the wheat genome integrating 2740 gene-associated single-nucleotide polymorphisms from the wheat iSelect assay, 1351 diversity array technology, 118 simple sequence repeat/sequence-tagged sites, and 416,856 genotyping-by-sequencing markers was developed. (g3journal.org)
Differential introgression of uniparentally inherited markers in bison populations with hybrid ancestries. (tamu.edu)

This constitutes the first report of using RH mapping to localize a gene in wheat and illustrates that this approach is feasible in a species with a large complex genome. (genetics.org)
For a fifteenth gene, Ppm1b, which had previously been assigned to RNO10, the map position was corrected to RNO6q12-q13. (deepdyve.com)
The gene-anchored high-resolution RH map (1 locus/300 kb) for the targeted region of BTA6 presented here will provide a valuable platform to guide high-quality assembling and annotation of the currently existing bovine genome sequence draft to establish the final architecture of BTA6. (biomedcentral.com)
However, the successful use of genome information from sequence-ready maps relies upon the precise identification and characterization of segments of conserved synteny, gene order, and evolutionary breakpoints in the bovine genome. (biomedcentral.com)
Using this method, we were able to map the human gene that complemented a recessive mutation in a CHO line to a region spanning less than 1 million bases (Mb) or 0.1% of the genome. (biomedcentral.com)
This cytogenetic map shows where a gene or marker lies relative to the stained bands (Fig. 8.8). (brainkart.com)
Cytogenetic maps are very low resolution compared with the other mapping techniques, yet they are useful to compare gene locations on a large scale. (brainkart.com)
In an effort to provide a rapid and robust tool for zebrafish gene mapping, a panel of radiation hybrids (RH) was produced by fusion of irradiated zebrafish AB9 cells with mouse B78 cells. (elsevier.com)
Mathematical, statistical, and computational principles relevant to this task are developed hand in hand with applications to gene mapping, risk prediction, and the testing of epidemiological hypotheses. (springer.com)
Comparative genetic mapping of the Gpr83 gene showed that it maps to regions of conserved synteny on mouse chromosome 9 (A2-3 region) and human chromosome 11 (q21 region). (nih.gov)
Genetic maps are a very important tool in evolutionary genomics and numerous practical applications like breeding, medical genetics, and gene cloning. (springer.com)
However, the fine mapping challenge, especially if the target is a gene of weak or moderate effect, requires large sample sizes and dense maps. (springer.com)
A gene-based radiation hybrid map of chicken microchromosome 14: comparison to human and alignment to the assembled chicken sequence. (inra.fr)
We present a gene-based RH map of the chicken microchromosome GGA14, known to have synteny conservations with human chromosomal regions HSA16p13.3 and HSA17p11.2. (inra.fr)
This gene is mapped to 9p13-p12. (wikipedia.org)
The human melanopsin gene consists of 10 exons and is mapped to chromosome 10q22. (jneurosci.org)
Morton NE and Collins A (1997) The future of gene mapping. (els.net)
Here, we applied the next-generation sequencing of a wheat doubled-haploid mapping population for high-resolution gene mapping and tested its utility for ordering shotgun sequence contigs of a flow-sorted wheat chromosome. (g3journal.org)
We demonstrate that the high-density reference map presented here is a useful resource for gene mapping and linking physical and genetic maps of the wheat genome. (g3journal.org)
The second generation of the International Equine Gene Mapping Workshop half-sibling linkage map. (tamu.edu)
cTnI gene locus maps to 19q13.4. (upmc.edu)
Altered gene dosage relationships are believed to contribute to hybrid performance. (plantcell.org)
We integrated these mapping data with genome-wide messenger RNA expression profiles as provided by SAGE (serial analysis of gene expression). (sciencemag.org)
The resulting Human Transcriptome Map generates gene expression profiles for any chromosomal region in 12 normal and pathologic tissue types. (sciencemag.org)
Isolation and characterization of interspecific heat‐resistant hybrids between a temperature sensitive Chinese hamster cell asparaginyl‐tRNA synthetase mutant and normal human leukocytes: Assignment of the human asnS gene to chromosome 18. (currentprotocols.com)
Assignment of the human mars gene, encoding methionyl‐tRNA synthetase, to chromosome 12 using human x Chinese hamster cell hybrids. (currentprotocols.com)
Based upon mapping of deletion breakpoints, putative tumour suppressor gene loci have been assigned to the distal part of the short arm of chromosome 1. (cancerindex.org)
Radiation hybrid mapping localized the hKv9.1 gene to 20q12 and the hKv9.3 gene to 2p24. (tcdb.org)

Mapmerge: merge genomic maps. (rwth-aachen.de)
Genomic location specified by transcript mapping, radiation hybrid mapping, genetic mapping or cytogenetic mapping. (nih.gov)
Radiation hybrids, derived through gamma irradiation of monosomic addition lines, have less than a complete maize chromosome in an oat genomic background. (springer.com)
Nonmodel systems can have biologic features-enforced outbreeding, absent or sparse linkage maps, complex genome structures, large numbers of repetitive elements, etc.-that directly hamper de novo assembly and therefore the general availability of genomic tools. (g3journal.org)

The E26C13 group was assigned to GGA25 (Gallus gallus chromosome 25) by FISH (fluorescence in situ hybridisation) mapping. (le.ac.uk)
Amarillo FIE, and Bass HW (2007) A transgenomic cytogenetic sorghum ( Sorghum propinquum ) bacterial artificial chromosome fluorescence in situ hybridization map of maize ( .Zea mays L.) pachytene chromosome 9, evidence for regions of genome hyperexpansion. (springer.com)
This sequence was localized on chromosome 10q21 by radiation hybrid mapping and fluorescence in situ hybridization. (biomedcentral.com)

2014) A comprehensive whole-genome integrated cytogenetic map for the alpaca (Lama pacos). (tamu.edu)

These stocks have been employed to map 8241 expressed sequence tags (ESTs) in wheat ( http://wheat.pw.usda.gov/NSF ). (genetics.org)
The ability to order previously deletion-binned but unordered ESTs by RH mapping approach. (genetics.org)
Two ESTs indicated by asterisks were placed in bins other than 1DL2-0.41-1.00, but were placed within this linkage group in the RH analysis and represent possible multiple-copy ESTs or simply deletion mapping errors. (genetics.org)
Additionally, with this small set of lines, almost all the tested ESTs could be mapped. (biomedcentral.com)
Radiation hybrid mapping overcomes these limitations by examining STSs or ESTs on original chromosomal fragments (Fig. 8.7). (brainkart.com)
o To map expressed sequence tags (ESTs) to BACs. (bio.net)

NIGMS human/rodent somatic cell hybrid mapping panels 1 and 2. (currentprotocols.com)
Characterization of NIGMS human/rodent somatic cell hybrid mapping panel 2 by PCR. (currentprotocols.com)

Construction of a 5000(rad) whole-genome radiation hybrid panel in the horse and generation of a comprehensive and comparative map for ECA11. (tamu.edu)

The results indicate that our physical mapping method based on radiation hybrids and array-CGH should significantly enhance the speed and efficiency of positional cloning in somatic cell genetics. (biomedcentral.com)
Recent advances in genome sequencing have facilitated the assembly of physical maps of candidate regions, but fine mapping and narrowing of candidate regions is still often tedious and remains a major obstacle in somatic cell genetics. (biomedcentral.com)
One of the major tools developed at early stages of genetics was genetic mapping. (springer.com)

o To play a central role in assembly of comparative physical maps of maize, sorghum, rice, and other grasses, by hybridization of mapped DNA probes to BAC grids. (bio.net)

Mapping of human chromosome 5 microsatellite polymorphisms. (currentprotocols.com)

abstract = "This chapter describes the use of radiation hybrids for constructing high-resolution maps of the human genome. (uthscsa.edu)

Comprehensive human genetic maps: individual and sex-specific variation in recombination. (nih.gov)
In this chapter we consider methods for building genetic maps (Section 6.1), various versions of "multiple" approach for QTL mapping (Section 6.2), and a new cost-effective method for genetic mapping based on selective DNA pooling (Section 6.3). (springer.com)
Relationship between the male (mcM) and female (fcM) genetic maps for chromosome 22. (els.net)
The 7296 unique mapping bins provided a five- to eight-fold higher resolution than genetic maps used in similar studies. (oregonstate.edu)
Polyploidy and abundance of repetitive DNA complicate the analysis of genetic variation and the development of high-density genetic maps of the wheat genome. (g3journal.org)

Radiation hybrids have recently been implemented for mouse mapping and have been found to have a higher level of resolution compared to that of interspecies meiotic mapping panels. (uthscsa.edu)
The chapter presents the advantages and disadvantages of the two different types of radiation hybrid panels. (uthscsa.edu)
As radiation hybrid mapping has become more popular, numerous laboratories have expressed an interest in gaining access to hybrid panels. (uthscsa.edu)
There is a renewed interest in total genome hybrid panels because they can be used without a piori knowledge of a marker's location. (uthscsa.edu)
Using radiation hybrid panels, the CAG/CTG repeat was mapped to chromosome 17q. (biomedsearch.com)
We realized that we could ask a different question from the one that radiation panels had previously explored," says Desmond J. Smith, M.D., Ph.D., professor of molecular and medical pharmacology at University of California, Los Angeles. (genengnews.com)
This is not the case for radiation mapping hybrid panels, where X-rays fragment the DNA and the resolution is very high," says Dr. Smith. (genengnews.com)
This unit provides an introduction to the various types of hybrid panels in use and reviews the status of commercially available panels. (currentprotocols.com)

and analyzing high-density genetic mapping data, preferably in a high-throughput environment. (bio.net)
Requires experience with tissue culture (plant or animal), and collection and analysis of mapping data, preferably as applied to radiation hybrids in a high-throughput environment. (bio.net)
The team used a high-throughput radiation hybrid mapping system that James' lab had developed for part of the world consortium Human Genome Project. (the-scientist.com)

Radiation hybrid maps remain useful for connectivity and orientation of sequenced contigs. (els.net)

Map-based positional cloning strategies can be used in somatic cell hybrids to analyze mutants with recessive phenotypes. (biomedcentral.com)
To achieve this goal, ING's Customer Journey Experts (CJEs) constantly map and analyze the way customers use ING services in a Customer Journey Map. (tudelft.nl)
The long-term goals of the Project are: 1) to create a physical map of maize anchored to the genetic map by screening and assembling bacterial artificial chromosome (BAC) clones, and 2) to design and augment informatics and database capabilities to collect, analyze, and disseminate the data publicly. (bio.net)

The book includes many topics currently accessible only in journal articles, including pedigree analysis algorithms, Markov chain Monte Carlo methods, reconstruction of evolutionary trees, radiation hybrid mapping, and models of recombination. (springer.com)

1998) Allelic association under map error and recombinational heterogeneity: a tale of two sites. (els.net)

Polymorphisms are not required to map a maize DNA fragment with this system. (springer.com)

Background - The resolution of radiation hybrid (RH) maps is intermediate between that of the genetic and BAC (Bacterial Artificial Chromosome) contig maps. (core.ac.uk)

Perhaps the first paper to give credible genome map lengths on different map scales. (els.net)
Yet the recent publication of a preliminary genome map of Rattus norvegicus and meetings at the National Institutes of Health have refocused attention on the rat. (the-scientist.com)

The RH procedure was used to map 14 DNA probes from a region of human chromosome 21 spanning 20 megabase pairs. (wikipedia.org)

Furthermore, the high-resolution sequence-referenced BTA6 map will enable precise identification of multi-species conserved chromosome segments and evolutionary breakpoints in mammalian phylogenetic studies. (biomedcentral.com)
Dr. Smith and colleagues proposed that radiation hybrid mapping data from mammalian cells could be used to delineate genetic survival networks for proliferation. (genengnews.com)

A) Comparison of maps generated by the recombination-based linkage, chromosome deletion bin, and radiation hybrid approach. (genetics.org)
Here we report on the construction of high-density and high-resolution radiation hybrid (RH) map of chromosome 4A supported by high-density chromosome deletion map. (k-state.edu)

Finally, this RH map was used for testing the accuracy of the chicken genome assembly for chromosome 5. (core.ac.uk)

Significantly higher mapping resolution in the centromeric region was observed as compared to recombination maps. (k-state.edu)

Mapper: an intelligent restriction mapping tool. (rwth-aachen.de)

The mutant rodent cells are fused with wild-type human fibroblasts and the resulting hybrids are irradiated and selected for absence (that is, complementation) of the mutant phenotype. (biomedcentral.com)

1990) Program description - Centre D'Etude Polymorphisme Humain (CEPH) - collaborative genetic mapping of the human genome. (els.net)

Ananiev E V, Riera-Lizarazu O, Rines HW, Phillips RL (1997) Oat-maize chromosome addition lines: A new system for mapping the maize genome. (springer.com)

o To construct positional cloning and sequence ready physical maps. (bio.net)

Wheat chromosome 1D RH map (right) as compared with the genetic (left) and the deletion map (middle). (genetics.org)
The surviving plants after irradiation were crossed to durum wheat (AABB), to produce pentaploid RH 1 s (AABBD), which allows the simultaneous mapping of the whole D-genome. (biomedcentral.com)
The results provided insight into various aspects of RH mapping in plants, including the genetically effective cell number for wheat (for the first time) and the potential implementation of this technique in other plant species. (biomedcentral.com)
A bioinformatical pipeline was developed for reliable variant analysis of sequence data generated for polyploid wheat mapping populations. (g3journal.org)
The results of variant mapping were consistent with the results obtained using the wheat 9000 SNP iSelect assay. (g3journal.org)

Ennis S, Maniatis N and Collins A (2001) Allelic association and disease mapping. (els.net)
2001) A high resolution radiation hybrid map of the human genome draft sequence. (els.net)

A high-resolution recombination map of the human genome. (nih.gov)

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