Loading...
  • Rad9
  • This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. (nih.gov)
  • Previous efforts of ours have focused on fission yeast S. pombe rad9, a gene that promotes gamma-ray resistance, UV-resistance, resistance to the DNA replication inhibitor hydroxyurea, and regulates the associated cell cycle checkpoints. (grantome.com)
  • Interestingly, we identified structurally and functionally similar paralogues of Rad9, which we call HRAD9B (human) and Mrad9B (mouse), indicating that Rad9 is part of a gene family. (grantome.com)
  • These data suggest that v- Src (show SRC Proteins ) attenuates ATR (show ANTXR1 Proteins )- Chk1 (show CHEK1 Proteins ) signaling through the inhibition of Rad17 - Rad9 (show RAD9A Proteins ) interaction. (antibodies-online.com)
  • RAD51
  • The results obtained regarding the study done with isolated lymphocytes exposed to GA (10 μM) for a 2 h period showed that GA up-regulated 1 gene for each donor, RAD51 for donor 1 and UNG for donor 2. (aacrjournals.org)
  • isoform
  • Isoform 3 and isoform 4 lack the C-terminal ligand-binding domain and may therefore constitutively activate thetranscription of a specific set of genes independently of steroidhormones. (uth.edu)
  • Proteins
  • Authors show that BRCA1 and RAD17 genes, whose derived proteins play a pivotal role in DNA damage repair, are transcriptional targets of gain-of-function mutant p53 (show TP53 Proteins ) proteins. (antibodies-online.com)
  • We found constitutively decreased levels of RAD9A and several other DNA repair proteins in two-cancer patients, compared to one-cancer patients. (uni-wuerzburg.de)
  • Conclusions/Significance: Collectively, our results support the idea that modulation of RAD9A and other cell cycle arrest and DNA repair proteins contribute to the risk of developing a second malignancy in childhood cancer patients. (uni-wuerzburg.de)
  • We found constitutively decreased levels of RAD9A and several other DNA repair proteins in two-cancer patients, compared to onecancer patients. (uni-wuerzburg.de)
  • Assay
  • Real-time PCR probe assay designed for gene expression analysis. (bio-rad.com)
  • Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay. (bio-rad.com)
  • pathway
  • In view of this, we studied gene expression in non-malignant mammary cell line (MCF10A) and in isolated human lymphocytes using a high-throughput technique, the RT 2 Profiler PCR arrays: Human DNA Damage Signaling Pathway. (aacrjournals.org)
  • The goal of this first pathway is to provide an overview of the most important gene products, processes and changes in cell condition elicited by the DNA damage response while keeping it clear and understandable. (wikipathways.org)
  • genomic
  • Specifically, we will make use of Mrad9 knockout cells and mice we constructed to address focused hypotheses designed to elucidate the mechanisms by which the gene maintains genomic stability, promotes resistance to DNA damage, and regulates spermatogenesis. (grantome.com)
  • Tumor
  • Tumor heterogeneity makes identification of genes most relevant to this process difficult. (aacrjournals.org)
  • Tumor cells were extracted from FFPE slides by laser capture microdissection and subjected to a qPCR array consisting of 84 genes participating in DNA damage response and repair (SA Biosystems, Qiagen). (aacrjournals.org)
  • Frequent amplification of this gene has been found in human tumors, which may be important for neoplastic transformation and tumor progression. (cancerindex.org)
  • mRNA
  • See reference mRNA sequence for the RAD9A gene (NM_004584.2). (nih.gov)
  • The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. (bioon.com.cn)
  • Quantification of mRNA expression by real-time RT PCR revealed lower RAD9A mRNA levels in both untreated and 1 Gy gamma-irradiated cells of two-cancer patients. (uni-wuerzburg.de)
  • ABL1
  • The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. (bioon.com.cn)
  • lipid peroxidation
  • This gene encodes a member of the aldehyde dehydrogenase family, a group of isozymes that may play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. (genecards.org)
  • qPCR
  • Gene expression was compared between the two samples, and select genes identified as significantly different were validated with qPCR. (aacrjournals.org)
  • ScienCell's GeneQuery™ Human DNA Damage Sensing qPCR Array Kit (GQH-DDS) is designed to facilitate gene expression profiling of 40 key genes involved in DNA damage sensing. (zqxzbio.com)
  • expression
  • Abstract 82: Gene expression induced by acrylamide and glycidamide in mammalian cells. (aacrjournals.org)
  • Presently there is a lot of information concerning the mechanisms of genotoxicity of AA and GA in mammalian cells, however this is not the case for the role of AA and GA in gene expression. (aacrjournals.org)
  • A value of p ≤0.05, and a fold change greater than 1.1 and lower than -1.1, were considered to be significant in gene expression dysregulation. (aacrjournals.org)
  • Concerning MCF10A cell line, the results obtained in 24h exposure showed that neither AA nor GA influenced the expression of the genes considered when compared to respective non-treated controls. (aacrjournals.org)
  • However, when individuals were pooled, no statistical significance results were observed, what may be related to the importance of inter-individual variability in gene expression. (aacrjournals.org)
  • Expression of genes of interest was also examined in 3 pairs of matched chemosensitive/chemoresistant ovarian cancer cell lines: A2780/A2780cp55, HeyA8/HeyA8MDR, and SKOV3ip1/SKOV3TRip2 both at baseline, and with 24-hour exposure to carboplatin. (aacrjournals.org)
  • Levels of gene expression were compared with the Wilcoxon signed-rank sum test for non-parametric data, and the student's t-test for normally distributed data. (aacrjournals.org)
  • Steroid hormone receptors are ligand-activatedtranscription factors that regulate eukaryotic gene expression andaffect cellular proliferation and differentiation in targettissues. (uth.edu)
  • Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis. (bio-rad.com)
  • In addition, we performed a genome-wide microarray analysis of both sisters and found several copy number variations, i.e., heterozygous deletion and reduced expression of the RSPO3 gene in the affected twin. (uni-wuerzburg.de)
  • cell
  • In the future, Western blots should be performed in order to analyze the protein levels associated with representative genes identified and further studies should be conducted in human lymphocytes concerning other exposure periods, to account for variations in reaction between cell lines and isolated human lymphocytes. (aacrjournals.org)
  • mRAD9
  • We constructed Mrad9 and Mrad9B knockout cells and mice and found that both genes are essential for embryogenesis. (grantome.com)