Rad17 is a subunit of the Rad9-Hus1-Rad1 clamp loader complex which is Dabigatran etexilate required for Chk1 activation after DNA damage. (healthdisparitiesks.org)
It is not clear whether Rad1, Rad9 and Hus1 also have distinct functional activities independent of the heterotrimeric form. (biomedcentral.com)
The protein products of several rad checkpoint genes of Schizosaccharomyces pombe (rad1 + , rad3 + , rad9 + , rad17 + , rad26 + , and hus1 + ) play crucial roles in sensing changes in DNA structure, and several function in the maintenance of telomeres. (elsevierpure.com)
The checkpoint clamp Rad9-Hus1-Rad1 (9-1-1) is loaded by the Rad17-RFC complex onto chromatin after DNA damage and plays a key role in the ATR-dependent checkpoint activation. (elsevierpure.com)
Moreover, we observed that hMYH was essential for the accumulation of hTopBP1 on damaged DNA, where hTopBP1 interacts with hRad9, a component of the Rad9-Hus1-Rad1 complex. (biomedcentral.com)
Human Rad1 is a component of a heterotrimeric PCNA like complex that also contains the Rad9 and Hus1 proteins. (biossusa.com)
The purified Rad17 and Rad9-Hus1-Rad1 (9-1-1) complexes can form a stable co-complex in the presence of ATP, using Rad17-Rad9 interactions. (reactome.org)
From computer modeling studies, the Rad17 subunit of the complex is also proposed to interact with the C-terminus of Rad1, p36 with the C-terminus of Hus1, and p38 with the C-terminus of Rad9. (reactome.org)
Subunit1
Our observations suggest that CK2 plays a crucial role in the ATR-dependent checkpoint pathway through its ability to phosphorylate Ser-341 and Ser-387 of the Rad9 subunit of the 9-1-1 complex. (elsevierpure.com)
abstract = "The protein products of several rad checkpoint genes of Schizosaccharomyces pombe (rad1+, rad3+, rad9+, rad17+, rad26+, and hus1+) play crucial roles in sensing changes in DNA structure, and several function in the maintenance of telomeres. (elsevierpure.com)
RAD13
The S. cerevisiae checkpoint protein Rad17, the orthologue of human Rad1, forms a homocomplex in response to treatment with DNA damaging agents, and the complex is required for yeast survival after exposure to genotoxic agents [ 12 ]. (biomedcentral.com)
To determine whether Rad1 functions to maintain genomic stability and prevent tumor development, we generated Mrad1 mutant mice by gene targeting. (biomedcentral.com)
This complex is believed to be involved in cellular responses to DNA damage, possibly by associating with Rad17 and several components of the PCNA-loading heteropentamer, replication factor C. Human Rad1 exhibits significant homology to Rad1 from S. pombe, and its expression in yeast rad1 mutants has been shown to partially restore radiation resistance and G2 checkpoint activity. (biossusa.com)
Pombe1
When the mammalian homologue of S. pombe Rad9 was inactivated, increases in chromosome end-to-end associations and frequency of telomere loss were observed. (elsevierpure.com)
Proteins2
Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. (wikipedia.org)
This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. (utsouthwestern.edu)
Protein encoded by this gene1
The protein encoded by this gene belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. (utsouthwestern.edu)
Entrez Gene1
Please see the Entrez Gene record to access additional publications. (avivasysbio.com)
Clamp1
The Rad17-RFC and 9-1-1 complexes are structurally similar to the RFC (replication factor C) clamp loader and PCNA sliding clamp, respectively, and similar mechanisms are thought to be used during loading of the 9-1-1 complex and PCNA. (reactome.org)
Replication2
It is constitutively associated with chromatin and is required for normal DNA replication (7 8 Rad17 protein Dabigatran etexilate was reported to be regulated by the ubiquitin-proteasome system (UPS) (9). (healthdisparitiesks.org)
During ATR signaling in response to DNA damage, Rad17 forms a complex with 9-1-1 and loads onto stalled replication forks [ 4 - 9 ]. (biomedcentral.com)
Mediates1
The anaphase-promoting complex (APC) was identified as the ubiquitin E3 ligase that mediates Rad17 degradation (9). (healthdisparitiesks.org)
Checkpoint control1
During meiosis in yeast and in mammals, RAD17 protein functions as a DNA damage sensor promoting DNA checkpoint control. (wikipedia.org)
Mammalian2
Furthermore, mammalian Rad9 interacted with Rad51, and inactivation of mammalian Rad9 also resulted in decreased homologous recombinational (HR) repair, which occurs predominantly in the S and G 2 phases of the cell cycle. (elsevierpure.com)
Together, these findings provide evidence of roles for mammalian Rad9 in telomere stability and HR repair as a mechanism for promoting cell survival after IR exposure. (elsevierpure.com)
Alternatively spliced transcript1
An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. (utsouthwestern.edu)
Tumor2
Knockdown of Rad9 in prostate tumor cells correlates with reduction of tumorigenicity in nude mice [ 16 ]. (biomedcentral.com)
It is likely that increased Rad9 expression is needed for proliferation of tumor cells by mechanisms such as getting beyond (tolerating) oncogene-induced replicative stress and enhancing DNA repair capability. (biomedcentral.com)
Vitro1
Here, we demonstrate that in vitro casein kinase 2 (CK2) specifically interacts with human 9-1-1 and phosphorylates serines 341 and 387 (Ser-341 and Ser-387) in the C-terminal tail of Rad9. (elsevierpure.com)
Heterotrimeric1
The 9-1-1 complex is a heterotrimeric ring-shaped structure that is loaded onto DNA by the Rad17-RFC complex. (reactome.org)
Mutations1
Mutations of this gene are associated with Seckel syndrome. (utsouthwestern.edu)
Yeast1
In yeast, the RAD17 protein facilitates proper assembly of the meiotic crossover recombination complex containing the RAD51 protein, thus promoting efficient repair of meiotic DNA double-strand breaks. (wikipedia.org)
Complex3
During male meiosis in maize (Zea mays), the ZmRAD17 gene is involved in repair of DNA double strand breaks, likely by promoting synaptonemal complex assembly. (wikipedia.org)
This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. (avivasysbio.com)
However, the presence of the 9-1-1 complex also alters the ability of Rad17 to become phosphorylated, perhaps suggesting that 9-1-1 may regulate the recruiment of additional ATR substrates. (reactome.org)
RefSeq1
Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. (avivasysbio.com)
This telomere instability correlated with enhanced S- and G 2 -phase- specific cell killing, delayed kinetics of γ-H2AX focus appearance and disappearance, and reduced chromosomal repair after ionizing radiation (IR) exposure, suggesting that Rad9 plays a role in cell cycle phase-specific DNA damage repair. (elsevierpure.com)
Function2
In this report we implicate a novel Dub ubiquitin-specific peptidase 20 (USP20) in the DDR by interacting with and regulating the protein level and function of Rad17. (healthdisparitiesks.org)
FUNCTION: [Summary is not available for the mouse gene. (utsouthwestern.edu)
Target1
XLS 97 kb) 12861_2017_153_MOESM8_ESM.xls (98K) GUID:?B2FE5241-0444-4D48-99D4-D5E55E4120EF Extra file 9: Desk S7: Target genes of the very best portrayed 18 miRNAs. (clinical-research-informatics.com)