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  • genotoxic stress
  • We also show that cdk2 is required for Spy1-enhanced cell viability and that Spy1 activates cdk2 under conditions of genotoxic stress. (aacrjournals.org)
  • A primary research interest of the Weiss lab centers on how mammalian cells maintain genomic stability under normal growth conditions and following genotoxic stress, and how loss of genomic integrity contributes to cancer and other diseases. (cornell.edu)
  • mammalian
  • Here we demonstrate that overexpression of human Spy1 enhances mammalian cell viability during cellular responses to DNA damage induced by genotoxic agents such as camptothecin, cisplatin, and hydroxyurea. (aacrjournals.org)
  • In this report we demonstrate that Spy1 expression in mammalian cells enhances survival under conditions of genotoxic damage, including treatments of CPT, cisplatin, and HU. (aacrjournals.org)
  • He lectures in the core veterinary curriculum, teaches a graduate course on genome maintenance mechanisms, and leads a research program aimed at dissecting how mammalian cells maintain genomic stability, using mouse models and an array of genetic, molecular, cell biological, and genomic technologies. (cornell.edu)
  • damage
  • Clonogenic survival assays and comet assays also show that Spy1 expression enhances cell survival after DNA damage. (aacrjournals.org)
  • We found that Spy1 can activate cdk2 during the DNA damage response and that expression of a dominant-negative form of cdk2 overrides Spy1 function in damaged cells. (aacrjournals.org)
  • Lastly, ablation of endogenous Spy1 expression using small interference RNA results in hypersensitization of cells to DNA damage. (aacrjournals.org)
  • DNA damage-inducible cell cycle checkpoints are complex signal transduction networks composed of cellular responses to genotoxic stresses. (aacrjournals.org)
  • Mutator phenotype of Caenorhabditis elegans DNA damage checkpoint mutants. (ebi.ac.uk)
  • As expected, deletions of these genes were sensitive to a broad range of DNA damage reagents. (phosphorylaseinhibitors.com)
  • Notably, deletion of trk1, a gene encoding the potassium ion transporter, caused strong sensitivity to almost all the DNA damage reagents used MLN2238 in our assay. (phosphorylaseinhibitors.com)
  • A major focus is on a trimeric DNA damage checkpoint complex called the 911 complex. (cornell.edu)
  • A related research endeavor addresses the regulation of DNA damage response mechanisms in germ cells and their impact on the origins of testicular germ cell tumors (TGCTs), which are unusual cancers in being highly sensitive to DNA damaging chemotherapeutics even at advanced stages. (cornell.edu)
  • Essential for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. (nih.gov)
  • During S phase the cell is more vulnerable to DNA damage than any other part of the cell cycle. (wikipedia.org)
  • In the presence of DNA damage, the cell can either repair the damage or induce cell death if the damage is beyond repair. (wikipedia.org)
  • These protein kinases send damage signals to the cell cycle control system to delay the progression of the cell cycle. (wikipedia.org)
  • apoptosis
  • Previously, differential proteomic and transcriptomic profiling in colorectal cancer cell lines and healthy colonic mucosa of patients with hereditary nonpolyposis colon cancer, respectively, have shown celecoxib-mediated expression changes in diverse cellular functions, including cell proliferation, apoptosis, immune function, and cell signaling ( 18 , 19 ). (aacrjournals.org)
  • genome
  • Human CLSPN genome location and CLSPN gene details page in the UCSC Genome Browser. (wikipedia.org)
  • In eukaryotes, the vast majority of DNA synthesis occurs during S phase of the cell cycle, and the entire genome must be unwound and duplicated to form two daughter copies. (wikipedia.org)
  • mutants
  • On the other hand, another 20 genes were found to be linked with DDR for the first time in this study, and the identities of corresponding mutants have been double checked. (phosphorylaseinhibitors.com)
  • overexpression
  • Furthermore, overexpression of HHUS1B in human 293 cells causes significant loss of clonogenicity, whereas similar studies with HHUS1 reveal no such effect. (aacrjournals.org)
  • control
  • The abundance of HRAD9B RNA, as judged by quantitative reverse transcription-PCR, is very low in most testicular tumors, particularly those of germ cell origin, i.e. , seminomas, relative to normal testis control, nonseminomas, or Leydig tumor cells. (aacrjournals.org)
  • These findings suggested that HHUS1 and HHUS1B have related but not identical functions in cell cycle checkpoint control and perhaps other as of yet undefined cellular processes. (aacrjournals.org)
  • molecular
  • In an attempt to better understand COX-2-independent molecular mechanisms underlying the chemopreventive activity of celecoxib, we did global transcription profiling of celecoxib-treated COX-2-positive and COX-2-deficient colorectal cancer cell lines. (aacrjournals.org)
  • In the future, in-depth work on selected genes will determine if these genes may serve as potential molecular targets for more effective chemopreventive strategies. (aacrjournals.org)
  • The use of COX-2 expresser and nonexpresser cell lines provides an opportunity to delineate the anticarcinogenic activities of celecoxib that are independent of COX-2 inhibition and may be instructive in identifying molecular profiles relevant for celecoxib-mediated adenoma regression. (aacrjournals.org)