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  • cdc42
  • Left panel) Exchange activity of immunoprecipitates from FLAG-ECT2-expressing cells (open symbols) or FLAG-expressing cells (filled symbols) on RhoA (circles), Rac1 (squares), or Cdc42 (triangles). (nih.gov)
  • In addition, using biochemical activity assays for Rho-like GTPases, we show that the expression of beta1A, beta1D, or IL2R-beta1A in GE11 or GD25 cells triggers activation of both RhoA and Rac1, but not of Cdc42. (nih.gov)
  • Downregulation of PLEKHA7 (zeige PLEKHA7 Antikörper ) in PACG may affect BAB (zeige RERE Antikörper ) integrity and aqueous humor outflow via its Rac1 / Cdc42 (zeige CDC42 Antikörper ) GAP activity, thereby contributing to disease etiology. (antikoerper-online.de)
  • Filamin C (zeige FLNC Antikörper ) promotes lymphatic invasion and lymphatic metastasis and increases cell motility by regulating Rac1 / cdc42 (zeige CDC42 Antikörper ) activites in esophageal squamous cell carcinoma. (antikoerper-online.de)
  • We found that Cdc42 associates with atypical PKCs (aPKCs) PKCζ and -λ in a GTP-dependent manner. (asm.org)
  • Most signals transmitted by Rho family members depend on the association of effector proteins with the GTP-bound form of Rac, Rho, or Cdc42. (asm.org)
  • The small GTP binding proteins Rac1 and Cdc42 are also regulators of cell spreading. (rupress.org)
  • Small Rho GTPases, such as Cdc42 and Rac1, have been implicated previously in neural stem/progenitor cell (NSPC) proliferation and neuronal maturation during embryonic development. (forskningsdatabasen.dk)
  • Here we used conditional genetic deletion and virus-based loss-of-function approaches to identify temporally distinct functions for Cdc42 and Rac1 in adult hippocampal neurogenesis. (forskningsdatabasen.dk)
  • Then, we progressed backwards up the signal transduction pathway and showed that the GTPase Rac1/Cdc42 and the protein Gα12 control the MAPK module, ATF2 phosphorylation, and AARE-dependent transcription. (pubmedcentralcanada.ca)
  • antibody
  • Smooth muscle -actin antibody (M 0851, 1:50, DAKO) binding was detected using biotin-streptavidin HRP kit (Zymed) and mounted in Immu-mount (Thermoscientific), or Alexafluor-594 goat anti-mouse (Invitrogen) and mounted as below. (cancercurehere.com)
  • Anti-striated muscle myosin antibody (MF20, 1:500 after heat retrieval, DSHB) binding was detected using Alexafluor goat anti-mouse (Invitrogen) and mounted as below. (cancercurehere.com)
  • ECT2 protein was detected with anti-ECT2 antibody in the cells as they progress through the cell cycle upon release from the drug arrest (upper left panel). (nih.gov)
  • This antibody is not suitable for use in a Reverse Phase Protein Array format as described in SOP M-105. (cancer.gov)
  • Genes
  • Many of the IEGs encode transcription factors whose expression is required for the activation of a class of delayed-response genes, which encode proteins that may contribute to the differentiation of NGF-treated PC12 cells ( 31 , 43 ). (asm.org)
  • At a molecular level, most of the results have been obtained by studying the transcriptional regulation of the activating transcription factor 3 ( ATF3 ), C/EBP homologous protein ( CHOP ), and asparagine synthetase ( ASNS ) genes. (pubmedcentralcanada.ca)
  • Pathway
  • Mechanistically, GTP-Rac1 was involved in SEMA3F mediated regulation of CRC cell stemness by targeting the Wnt/β-catenin pathway. (cancerindex.org)
  • PKCs and Rho family GTP-binding proteins both have effects on the actin cytoskeleton, the activation of p70 S6 kinase, the JNK pathway, and the fos and NF-κβ promoters ( 44 ). (asm.org)
  • Exogenous expression of phosphomimetic S177D-Pdlim5 inhibits cell migration and attenuates lamellipodia formation.Notably, S177D-Pdlim5, but not WT-Pdlim5, attenuates the association with Rac1-specific guanine nucleotide exchange factors at the cell periphery.Taken together, our findings indicate that phosphorylation of Pdlim5 on Ser177 by AMPK mediates inhibition of cell migration by suppressing the Rac1-Arp2/3 signalling pathway. (nih.gov)
  • Taken together, our findings indicate that phosphorylation of Pdlim5 on Ser177 by AMPK mediates inhibition of cell migration by suppressing the Rac1-Arp2/3 signalling pathway. (nih.gov)
  • Silencing Ras-Related C3 Botulinum Toxin Substrate 1 Inhibits Growth and Migration of Hypopharyngeal Squamous Cell Carcinoma via the P38 Mitogen-Activated Protein Kinase Signaling Pathway. (ptglab.com)
  • We identified the mitogen-activated protein kinase (MAPK) module MEKK1/MKK7/JNK2 as the pathway responsible for ATF2 phosphorylation on the threonine 69 (Thr69) and Thr71 residues. (pubmedcentralcanada.ca)
  • inhibition
  • We also show that genetic deletion or pharmacological inhibition of Rac1 in NRas(Q61K) induced melanoma suppresses tumor growth, lymph node spread, and tumor cell invasiveness, suggesting a potential value for Rac1 as a therapeutic target for activated NRas-driven tumor growth and invasiveness. (nih.gov)
  • RAC1 inhibition as a therapeutic target for gefitinib-resistant non-small-cell lung cancer. (nih.gov)
  • Finally, to investigate whether RAC1 inhibition could suppress tumor growth in vivo, we inoculated a human gefitinib-resistant NSCLC cell line, RPC-9 cells, subcutaneously into mice. (nih.gov)
  • Furthermore, selective inhibition of Rac1 activation in BLA immediately following fear conditioning impaired STM and LTM, demonstrating that fear conditioning-induced Rac1 activation in BLA plays a critical role in the formation of both STM and LTM of conditioned fear. (frontiersin.org)
  • Mutation
  • Taken together, these results indicate that DOCK1 is a critical regulator of the malignant phenotypes induced by Rac1 (P29S), and suggest that targeting DOCK1 might be an effective approach to treat cancers associated with Rac1 (P29S) mutation. (antikoerper-online.de)
  • Mutation or deletion of each PU.1 site decreased promoter activity, and the level of activity mediated by each site correlated with its binding avidity for PU.1, as determined by gel shift competition assays. (bloodjournal.org)
  • Transfection
  • 9-20 Reconstitution of the oxidase by transfection into cell lines deficient in the oxidase proteins or recombination of the components in vitro shows that efficient superoxide generation by the NADPH oxidase complex is absolutely dependent on these components. (bloodjournal.org)
  • small
  • The small GTP-binding protein Rac1, a member of the Rho family of small GTPases, has been implicated in regulation of many cellular processes including adhesion, migration and cytokinesis. (cancercurehere.com)
  • Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. (abcam.com)
  • Auf www.antikoerper-online.de finden Sie aktuell 17 Ras-Related C3 Botulinum Toxin Substrate 1 (Rho Family, Small GTP Binding Protein Rac1) (RAC1) Antikörper von 3 unterschiedlichen Herstellern. (antikoerper-online.de)
  • RalA is one of two proteins in the Ral family, which is itself a subfamily within the Ras family of small GTPases. (wikipedia.org)
  • The Rho family of small GTP-binding proteins plays a central role in regulating actin polymerization, the formation of cellular structures dependent on actin, and transformation by some oncogenes ( 21 ). (asm.org)
  • Rac1, a member of the Rho family of small GTPases, is crucial for morphological changes of the mature neuronal synapse including spine formation and activity-dependent spine enlargement, while its role in the formation of associated memories, such as conditioned fear memory, is not clear. (frontiersin.org)
  • suppresses
  • Semaphorin-3F suppresses the stemness of colorectal cancer cells by inactivating Rac1. (cancerindex.org)
  • Consistent with this observation, S177D-Pdlim5 suppresses Rac1 activity at the cell periphery and displaces the Arp2/3 complex from the leading edge. (nih.gov)
  • assay
  • Interestingly, the activity of smgGDS toward Rac1 could not be observed in a [3H]GDP/GTP exchange assay under conditions where it stimulated incorporation of GTP[gamma S] into Rac1. (biochemj.org)
  • b) F-actin binding assay of AMPK. (nih.gov)
  • Next, we performed an F-actin-binding assay, in which F-actin and its binding proteins are found in the pellet fraction, to determine whether Pdlim5 promotes the recruitment of AMPK onto actin filaments. (nih.gov)
  • mice
  • Cardiac Rac1 overexpression in mice creates a substrate for atrial arrhythmias characterized by structural remodelling. (biomedsearch.com)
  • METHODS AND RESULTS: In mice with cardiac overexpression of constitutively active Rac1 (RacET), statin-treated RacET, and wild-type controls (age 6 months), conduction in the right and left atrium (RA and LA) was mapped epicardially. (biomedsearch.com)
  • CONCLUSION: The substrate for atrial arrhythmias in mice with Rac1 overexpression is characterized by conduction disturbances and atrial fibrosis. (biomedsearch.com)
  • Mice expressing melanocyte lineage NRasQ61K retain pigmentation defects caused by Rac1 deletion. (nih.gov)
  • h) Ventral skin from P14 NRas Ctr and Rac1 f/f NRas mice with anti-dopachrome tautomerase (melanocytes). (nih.gov)
  • 2011). At P14, Tyr∷NRasQ61K Rac1 f/f Tyr∷Cre mice had similar pigmentation patterns to Rac1 f/f Tyr∷Cre mice (Figure 2a-d and Supplementary Figure S3A and B online;Li et al. (nih.gov)
  • 2011). Furthermore, the number and size of white patches on the dorsal skin between Tyr∷NRasQ61K Rac1 f/f Tyr∷Cre and Rac1 f/f Tyr∷Cre mice was similar (Figure 2a and e and Supplementary Figure S3A online). (nih.gov)
  • therapeutic
  • Our findings demonstrate the ability of SEMA3F to inhibit the stemness of human CRC cells by suppressing Rac1 activation, which suggests a novel therapeutic approach for CRC. (cancerindex.org)
  • deletion
  • Here, we report that selective deletion of Rac1 in excitatory neurons, but not in parvalbumin inhibitory neurons, impaired short- and long-term memories (STM and LTM) of fear conditioning. (frontiersin.org)
  • tyrosine
  • TrkA, like other growth factor receptors, is a tyrosine kinase that is activated upon ligand binding and receptor dimerization ( 37 , 39 ). (asm.org)
  • cancers
  • Ral proteins have been associated with the progression of several cancers, including bladder cancer and prostate cancer. (wikipedia.org)
  • phosphatase
  • Lysates of cells arrested at G1 phase by aphidicolin or at M phase by nocodazole were incubated with a protein phosphatase VHR, separated by SDS-PAGE, and analyzed for ECT2 (lower left panel). (nih.gov)
  • pathogenesis
  • CSTF2-induced shortening of the RAC1 3'UTR promotes the pathogenesis of urothelial carcinoma of the bladder. (ptglab.com)
  • Activation
  • 21-23 Moreover, p40 phox can bind p67 phox and p47 phox , and the strong binding of p40 phox to p67 phox can be disrupted by the activated guanosine triphosphate-bound form of Rac, a key intermediate in oxidase activation. (bloodjournal.org)
  • Furthermore, recent studies have indicated that activation of a number of membrane-bound receptors and intracellular signaling molecules in BLA is required for fear conditioning. (frontiersin.org)
  • heterodimer
  • Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). (abcam.com)
  • promotes
  • These findings indicate that Pdlim5 binds AMPK directly and promotes the recruitment of AMPK onto F-actin, a process mediated by α-actinin. (nih.gov)