• Multiple alternatively spliced transcript variants have been identified for this gene which encode different protein isoforms. (abnova.com)
  • Rabs cycle between a GTP-bound "on" state and a GDP-bound "off" state, which are structurally distinguished by the conformations of two flexible regions known as switch I and switch II. (pnas.org)
  • The C-terminal prenylated GDP-bound Rabs are extracted from the membrane into the cytosol by GDP-dissociation inhibitors (GDIs). (pnas.org)
  • Analysis of DENND1-Rab35, Rabex-Rab5, TRAPP-Rab1 and DrrA-Rab1 suggests Rabs have the potential for activation by distinct GDP-release pathways. (ox.ac.uk)
  • Like other GTPases, Rabs switch between two conformations, an inactive form bound to GDP (guanosine diphosphate), and an active form bound to GTP (guanosine triphosphate). (wikipedia.org)
  • The loss of the GDI1 gene has been shown through brain extracts of the mutant mice to lead to the accumulation of the Rab4 and Rab5 proteins, thus inhibiting their function. (wikipedia.org)
  • AT 1 interacts with multiple heterotrimetric G proteins, including G q/11 , G i , G 12 , and G 13 , and produces second messengers, such as inositol triphosphate, diacylglycerol, and reactive oxygen species (ROS). (ahajournals.org)
  • Unlike the host GDF PRA1, a polytopic membrane protein ( 13 ) that binds to both Rab and GDI ( 14 , 15 ), SidM interacts only with Rab1 ( 16 ) ( Fig. S1 ), suggesting a distinct mechanism underlying SidM-catalyzed GDI displacement. (pnas.org)
  • Here we report for the first time that the rab1b protein interacts specificially with GM130 in a GTP‐dependent manner and therefore needs the hypervariable regions of the N‐ and C‐termini. (embopress.org)
  • Using the yeast two‐hybrid approach we surprisingly identified GM130 as a protein that interacts directly and in a GTP‐dependent manner with rab1b. (embopress.org)
  • REPs carry only the GDP-bound form of Rab, and Rab effectors, proteins with which Rab interacts and through which it functions, only bind the GTP-bound form of Rab. (wikipedia.org)
  • Glutamate transporter associated protein 3-18 (GTRAP3-18) is an endoplasmic reticulum (ER)-localized protein belonging to the prenylated rab-acceptor-family interacting with small Rab GTPases, which regulate intracellular trafficking events. (nih.gov)
  • A GDP dissociation inhibitor (GDI) binds the prenyl groups of the inactive, GDP-bound form of Rab, inhibits the exchange of GDP for GTP (which would reactivate the Rab) and delivers Rab to its original membrane. (wikipedia.org)
  • They can be detected in an inactive or an active conformation depending on the nucleotidebound status, and are considered as switches, which cycle between an active, membrane‐associated and an inactive cytosolic status ( Olkkonen and Stenmark, 1997 ). (embopress.org)
  • The specific binding of the effector to the Rab protein allows the Rab protein to be effective, and conversely, the conformation shift of the Rab protein to the inactive state leads to effector dissociation from the Rab protein. (wikipedia.org)
  • Two of them encode large protein fragments highly homologous to rat GM130 and to human Golgin95. (embopress.org)
  • Also, other proteins which involved in the same pathway with Rab1 were listed below. (creativebiomart.net)
  • Furthermore, Rab1 switch II glutamine mutants refractory to activation by DrrA can be activated by TRAPP, showing that a single Rab can be activated by more than one mechanistically distinct GDP-release pathway. (ox.ac.uk)
  • Rab1 is a small GTPase regulating vesicular traffic between early compartments of the secretory pathway. (rupress.org)
  • In the yeast Saccharomyces cerevisiae, many of the ATG proteins needed for macroautophagy are shared with the biosynthetic cytoplasm to the vacuole-targeting (CVT) pathway that transports certain hydrolases into the vacuole. (wikipedia.org)
  • In the macroautophagy pathway, Rab1 mediates the recruitment of Atg1 to the PAS. (wikipedia.org)
  • It is noted that the social and behavioral patterns exhibited in mice that are carriers of the GDI1 protein are similar to those observed in humans with the same deletion. (wikipedia.org)
  • Coatomer subunit beta is a protein that in humans is encoded by the COPB1 gene. (wikipedia.org)
  • We mapped the rab1b binding site of GM130 and provide evidence that it is different to the previously described p115 and Grasp65 binding sites of the GM130 protein. (embopress.org)
  • Purified SidM specifically and directly bound to PtdIns(4)P, whereas the SidM-interacting Icm/Dot substrate LidA preferentially bound PtdIns(3)P but also PtdIns(4)P, and the L. pneumophila Arf1 GEF RalF did not bind to any PIs. (nih.gov)
  • The PtdIns(4)P-binding domain of SidM was mapped to the 12-kDa C-terminal sequence, termed "P4M" (PtdIns4P binding of SidM/DrrA). (nih.gov)
  • Identification of SidM as a PI-binding Protein-The signaling lipid PtdIns(4)P was recently discovered to be specifically recognized by the Icm/Dot substrate SidC on LCVs (32, 33). (nih.gov)
  • SidM is the only known protein that catalyzes both the exchange of GDP for GTP and GDI displacement from small GTPase Rab1. (pnas.org)
  • We determined the crystal structures of SidM alone (residues 317-647) and SidM (residues 193-550) in complex with nucleotide-free WT Rab1. (pnas.org)
  • The SidM structure contains an N-terminal helical domain with a potential new function, a Rab1-activation domain, and a C-terminal phosphatidylinositol 4-phosphate-binding P4M domain. (pnas.org)
  • The Rab1-activation domain has extensive strong interactions mainly with Rab1 switch I and II regions that undergo substantial conformational changes on SidM binding. (pnas.org)
  • LidA specifically binds to Rab-GTP and collaborates with SidM to recruit early secretory vesicles ( 9 ). (pnas.org)
  • Implicit in the presence of a putative MAPKKK in the transduction sequence is that protein phosphorylation via a mitogen-activated protein kinase (MAPK) cascade(s) is involved in mediating responses to ethylene. (plantphysiol.org)
  • We map LepB GAP domain to residues 313-618 and show that the GAP domain is Rab1 specific with a catalytic activity higher than the canonical eukaryotic TBC GAP and the newly identified VirA/EspG family of bacterial RabGAP effectors. (nih.gov)
  • Both effectors are colocalized with Rab1 on LCVs. (pnas.org)
  • The mutant protein required posttranslational isoprenylation for inhibition and behaved as a competitive inhibitor of wild-type rab1 function. (rupress.org)
  • Glu449, structurally equivalent to TBC RabGAP glutamine finger in apo-LepB, undergoes a drastic movement upon Rab1 binding, which induces Rab1 Gln70 side-chain flipping towards GDP-AlF3 through a strong ionic interaction. (nih.gov)
  • Our studies have demonstrated that adenovirus-driven expression of Rab1 promotes cell growth of primary cultures of neonatal cardiomyocytes in vitro and that transgenic expression of Rab1 in the myocardium induces cardiac hypertrophy in mouse hearts in vivo. (elsevier.com)
  • Ras mediates its effect on cell proliferation mainly by activation of its effector Raf to initiate the mitogen-activated protein kinase (MAPK/extracellular signal regulated kinase [ERK]) cascade. (ahajournals.org)
  • TBC1D20 is the ER-localized GAP for Rab1, and is the only GAP blocking the delivery of secretory cargo from the ER to the cell surface. (ox.ac.uk)
  • LepB GAP domain is also structurally distinct from the VirA/EspG family of bacterial RabGAPs that adopt an α/β fold despite that they are also of prokaryotic origin and highly specific for Rab1. (nih.gov)
  • In this review, we describe detailed mechanisms of signal transduction pathways of Ang II involving small G proteins in VSMCs together with their functional significances in mediating vascular remodeling. (ahajournals.org)
  • As distinct from animal cells comprising 40 subfamilies of Rab proteins, which are the key regulators of intracellular vesicular transport, numerous Rab proteins in Arabidopsis and other plant species could be grouped in only eight subfamilies on the basis of their functional properties. (deepdyve.com)
  • Using a set of GM130 deletion mutants we also demonstrate that the rab1b binding is independent of the N‐ and C‐termini of GM130. (embopress.org)